Mutagenetix > Incidental Mutation

Incidental Mutation 'R9606:Mov10'

723858

Institutional Source

Beutler Lab

Gene Symbol

Mov10

Ensembl Gene

ENSMUSG00000002227

Gene Name

Moloney leukemia virus 10

Synonyms

Mov-10

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.381) question?

Stock #

R9606 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

104794836-104818563 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 104800348 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 570 (V570D)

Ref Sequence

ENSEMBL: ENSMUSP00000128246 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000002297] [ENSMUST00000106774] [ENSMUST00000106775] [ENSMUST00000136148] [ENSMUST00000166979] [ENSMUST00000168015]

AlphaFold

P23249

Predicted Effect

probably benign
Transcript: ENSMUST00000002297
AA Change: V570D

PolyPhen 2 Score 0.096 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000002297
Gene: ENSMUSG00000002227
AA Change: V570D

Domain	Start	End	E-Value	Type
low complexity region	297	312	N/A	INTRINSIC
low complexity region	338	353	N/A	INTRINSIC
AAA	517	699	5.72e-3	SMART
low complexity region	953	970	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106774

SMART Domains

Protein: ENSMUSP00000102386
Gene: ENSMUSG00000002227

Domain	Start	End	E-Value	Type
low complexity region	297	312	N/A	INTRINSIC
low complexity region	338	353	N/A	INTRINSIC
AAA	517	699	5.72e-3	SMART
low complexity region	953	970	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106775
AA Change: V643D

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000102387
Gene: ENSMUSG00000002227
AA Change: V643D

Domain	Start	End	E-Value	Type
low complexity region	297	312	N/A	INTRINSIC
low complexity region	338	353	N/A	INTRINSIC
AAA	517	699	5.72e-3	SMART
low complexity region	953	970	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000136148

Predicted Effect

probably benign
Transcript: ENSMUST00000166979
AA Change: V643D

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000126897
Gene: ENSMUSG00000002227
AA Change: V643D

Domain	Start	End	E-Value	Type
low complexity region	61	75	N/A	INTRINSIC
low complexity region	370	385	N/A	INTRINSIC
low complexity region	411	426	N/A	INTRINSIC
AAA	590	772	5.72e-3	SMART
low complexity region	1026	1043	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000168015
AA Change: V570D

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000128246
Gene: ENSMUSG00000002227
AA Change: V570D

Domain	Start	End	E-Value	Type
low complexity region	297	312	N/A	INTRINSIC
low complexity region	338	353	N/A	INTRINSIC
AAA	517	699	5.72e-3	SMART
low complexity region	953	970	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous knockout is embryonic lethal. Heterozygous knockout leads to reduced dendritic branching of neurons, which affects anxiety- and/or activity-related behavior. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6430548M08Rik	A	G	8: 120,153,967	Y286C	probably damaging	Het
A530032D15Rik	G	C	1: 85,097,623		probably null	Het
Cacna1c	T	C	6: 118,610,494	T1657A		Het
Ccs	A	T	19: 4,832,869	D151E	probably benign	Het
Cfap74	A	G	4: 155,424,676	T295A		Het
Chmp4c	A	T	3: 10,367,162	E44V	probably damaging	Het
Crybg2	A	T	4: 134,074,072	K539*	probably null	Het
Dgkg	T	C	16: 22,622,261	M1V	probably null	Het
E2f2	T	A	4: 136,184,432	Y251*	probably null	Het
Fat2	T	C	11: 55,289,267	Y1416C	probably damaging	Het
Fgl2	T	C	5: 21,372,993	S93P	possibly damaging	Het
Hivep3	C	T	4: 120,132,589	T2079I	probably damaging	Het
Igfl3	A	C	7: 18,179,995	K90T	possibly damaging	Het
Lsamp	A	G	16: 41,888,929	D74G	probably benign	Het
Mmrn2	C	A	14: 34,397,697	T211K	possibly damaging	Het
Mrc1	A	T	2: 14,308,706	Y916F	probably benign	Het
Myo10	CAGGTATAAAG	CAG	15: 25,776,315		probably null	Het
Ndc1	T	A	4: 107,389,489	C370S	probably damaging	Het
Nln	TGGTCCAGGTAAAACTGCCCCAGCCAATCAGGTACCTTGGATAGAGGTCCAGGTAAAACTGCCCCAGCCAATCAGGTACCTTGGATAGAGGTCCAGGTAGAACTGCCCCAGC	TGGTCCAGGTAAAACTGCCCCAGCCAATCAGGTACCTTGGATAGAGGTCCAGGTAGAACTGCCCCAGC	13: 104,050,416		probably null	Het
Nrcam	A	T	12: 44,562,457	N455I	probably damaging	Het
Nrp1	G	A	8: 128,502,548	E836K	probably benign	Het
Olfr1477	A	T	19: 13,502,579	K79*	probably null	Het
Olfr329-ps	C	T	11: 58,542,927	C196Y	probably damaging	Het
Olfr812	A	T	10: 129,842,756	C95*	probably null	Het
Olfr915	A	G	9: 38,647,324	S67P	probably damaging	Het
Pcca	T	C	14: 122,664,305	S287P	probably damaging	Het
Pitpnm3	T	A	11: 72,064,243	T482S	probably benign	Het
Pld2	C	T	11: 70,555,067	R676*	probably null	Het
Ppm1k	T	C	6: 57,514,072	Y286C	possibly damaging	Het
Rb1	T	C	14: 73,280,133	N278D	probably damaging	Het
Sema3g	T	C	14: 31,221,826	L221P	probably damaging	Het
Skint2	C	T	4: 112,625,950	T184I	probably benign	Het
Slc38a2	A	G	15: 96,693,291	F232S	probably damaging	Het
Snx6	T	C	12: 54,768,026	N170S	probably benign	Het
Sox9	T	A	11: 112,782,590	N2K	probably damaging	Het
Srpk2	T	C	5: 23,524,606	E345G	probably benign	Het
Tbc1d21	T	C	9: 58,361,204	M291V	possibly damaging	Het
Tekt2	T	C	4: 126,324,900	N20S	probably benign	Het
Tnxb	T	G	17: 34,695,604	I1888S	possibly damaging	Het
Tsnaxip1	G	A	8: 105,840,053	E174K	probably damaging	Het
Ttc28	G	A	5: 111,285,274	R2058Q	probably benign	Het
Ttn	C	T	2: 76,885,013	E7912K	unknown	Het
Ttyh2	T	A	11: 114,710,841	D430E	probably benign	Het
Ubr2	C	T	17: 46,934,094	G1669R	probably damaging	Het
Vcan	A	T	13: 89,705,372	S490T	probably damaging	Het
Vmn1r86	T	A	7: 13,102,814	D45V	probably damaging	Het
Wdr12	A	G	1: 60,088,067	V143A	possibly damaging	Het
Zbtb18	T	A	1: 177,447,423	Y116*	probably null	Het
Zdbf2	C	T	1: 63,303,377	T305I	possibly damaging	Het
Zfp384	T	A	6: 125,030,839	M244K	possibly damaging	Het
Zfp423	A	T	8: 87,687,967	V1241D	probably damaging	Het
Zfp574	A	G	7: 25,081,215	Y554C	probably damaging	Het
Zfp616	T	C	11: 74,085,394	S830P	probably damaging	Het
Zfp839	A	G	12: 110,868,342	Y677C	probably benign	Het

Other mutations in Mov10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00498:Mov10	APN	3	104800947	splice site	probably benign
IGL01111:Mov10	APN	3	104801405	missense	possibly damaging	0.71
IGL01315:Mov10	APN	3	104795945	missense	probably damaging	0.98
IGL01463:Mov10	APN	3	104800324	missense	probably damaging	1.00
IGL02114:Mov10	APN	3	104795318	unclassified	probably benign
IGL02354:Mov10	APN	3	104804121	splice site	probably benign
IGL02361:Mov10	APN	3	104804121	splice site	probably benign
IGL02692:Mov10	APN	3	104800803	nonsense	probably null
IGL03104:Mov10	APN	3	104797307	missense	probably damaging	1.00
IGL03121:Mov10	APN	3	104801002	missense	probably benign
P0040:Mov10	UTSW	3	104804679	missense	probably damaging	1.00
R0025:Mov10	UTSW	3	104804603	missense	probably damaging	1.00
R0270:Mov10	UTSW	3	104795405	missense	probably benign	0.09
R0747:Mov10	UTSW	3	104802496	missense	probably benign	0.41
R1434:Mov10	UTSW	3	104795174	missense	probably damaging	1.00
R1482:Mov10	UTSW	3	104804546	missense	probably damaging	0.98
R1594:Mov10	UTSW	3	104795411	missense	probably damaging	1.00
R1656:Mov10	UTSW	3	104799596	missense	probably benign	0.03
R1739:Mov10	UTSW	3	104800282	missense	probably damaging	0.98
R1785:Mov10	UTSW	3	104818116	missense	possibly damaging	0.73
R1786:Mov10	UTSW	3	104818116	missense	possibly damaging	0.73
R1911:Mov10	UTSW	3	104801560	splice site	probably benign
R1962:Mov10	UTSW	3	104796977	missense	probably damaging	1.00
R1993:Mov10	UTSW	3	104799419	missense	probably damaging	1.00
R2095:Mov10	UTSW	3	104801531	missense	probably damaging	1.00
R2138:Mov10	UTSW	3	104804242	missense	probably benign	0.00
R3107:Mov10	UTSW	3	104799724	missense	probably damaging	1.00
R4241:Mov10	UTSW	3	104797276	missense	probably benign	0.45
R4280:Mov10	UTSW	3	104799779	missense	probably damaging	0.98
R4474:Mov10	UTSW	3	104818465	missense	probably damaging	1.00
R5227:Mov10	UTSW	3	104802578	missense	probably benign
R5391:Mov10	UTSW	3	104802533	missense	probably benign	0.12
R5704:Mov10	UTSW	3	104799596	missense	probably benign	0.03
R5819:Mov10	UTSW	3	104801512	missense	probably damaging	1.00
R5842:Mov10	UTSW	3	104799379	splice site	probably benign
R6059:Mov10	UTSW	3	104817950	utr 3 prime	probably benign
R6692:Mov10	UTSW	3	104818044	missense	probably damaging	0.97
R7226:Mov10	UTSW	3	104801012	missense	probably damaging	1.00
R7426:Mov10	UTSW	3	104800052	splice site	probably null
R7633:Mov10	UTSW	3	104797065	missense	possibly damaging	0.93
R7637:Mov10	UTSW	3	104795885	missense	probably benign	0.26
R7869:Mov10	UTSW	3	104804678	missense	probably damaging	1.00
R8684:Mov10	UTSW	3	104804374	missense	probably benign
R9008:Mov10	UTSW	3	104800016	missense	probably benign	0.09
R9127:Mov10	UTSW	3	104804343	nonsense	probably null
R9559:Mov10	UTSW	3	104800961	missense
R9587:Mov10	UTSW	3	104804583	missense	probably benign	0.11
R9602:Mov10	UTSW	3	104800968	missense	probably benign	0.18
R9708:Mov10	UTSW	3	104797297	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TCCCCTTGGCTATGAACCAC -3'
(R):5'- GACTAGCAGACATCTGGAAGGC -3'

Sequencing Primer
(F):5'- CTCCTTTTGCAGACAAGGAAACTG -3'
(R):5'- AGGCAAACGCAGTCTGAGTCC -3'

Posted On

2022-09-12