Mutagenetix > Incidental Mutation

Incidental Mutation 'R0153:Amn1'

22834

Institutional Source

Beutler Lab

Gene Symbol

Amn1

Ensembl Gene

ENSMUSG00000068250

Gene Name

antagonist of mitotic exit network 1

Synonyms

MMRRC Submission

038436-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.117) question?

Stock #

R0153 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

149157147-149188712 bp(-) (GRCm38)

Type of Mutation

utr 5 prime

DNA Base Change (assembly)

G to T at 149188593 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000116060 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095319] [ENSMUST00000111535] [ENSMUST00000141346]

AlphaFold

B8JKV0

Predicted Effect

probably benign
Transcript: ENSMUST00000095319

SMART Domains

Protein: ENSMUSP00000092957
Gene: ENSMUSG00000068250

Domain	Start	End	E-Value	Type
LRR	17	41	2.82e0	SMART
LRR	42	70	1.52e2	SMART
LRR	71	96	1.25e-1	SMART
LRR	97	122	3.89e-3	SMART
LRR	123	147	1.44e1	SMART
LRR	150	175	1.28e1	SMART
Blast:LRR	176	204	3e-10	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000111535

SMART Domains

Protein: ENSMUSP00000107160
Gene: ENSMUSG00000068250

Domain	Start	End	E-Value	Type
LRR	60	84	2.82e0	SMART
LRR	85	113	1.52e2	SMART
LRR	114	139	1.25e-1	SMART
LRR	140	165	3.89e-3	SMART
LRR	166	190	1.44e1	SMART
LRR	193	218	1.28e1	SMART
Blast:LRR	219	247	4e-10	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000141346

SMART Domains

Protein: ENSMUSP00000116060
Gene: ENSMUSG00000068250

Domain	Start	End	E-Value	Type
LRR	17	41	2.82e0	SMART
LRR	42	70	1.52e2	SMART
LRR	71	96	1.25e-1	SMART
LRR	97	121	1.44e1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149149

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150610

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152062

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203043

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000204681

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 98.9%
3x: 98.0%
10x: 95.5%
20x: 90.1%

Validation Efficiency

97% (99/102)

Allele List at MGI

Other mutations in this stock

Total: 85 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1190002N15Rik	G	T	9: 94,524,480	D291E	probably benign	Het
2010300C02Rik	A	G	1: 37,624,639	V726A	probably benign	Het
Abcb9	T	C	5: 124,080,056	M406V	probably benign	Het
Adar	T	C	3: 89,730,814	S2P	probably benign	Het
Adgre1	T	A	17: 57,443,939	S538T	possibly damaging	Het
Alms1	T	A	6: 85,641,381	I2803N	possibly damaging	Het
Arid1b	G	A	17: 5,342,932	A2246T	probably damaging	Het
BC024139	T	C	15: 76,121,747	E418G	probably damaging	Het
Bok	A	G	1: 93,686,517	D24G	probably damaging	Het
Cabp2	T	C	19: 4,084,913		probably benign	Het
Ccdc141	C	A	2: 77,165,238		probably benign	Het
Ccdc178	T	C	18: 22,150,435	T13A	probably benign	Het
Ccdc42	G	T	11: 68,587,650	V33F	possibly damaging	Het
Clcn7	G	A	17: 25,149,202		probably benign	Het
Cluh	A	G	11: 74,657,350		probably benign	Het
Cr1l	A	T	1: 195,114,856		probably benign	Het
Csnk1g3	T	A	18: 53,918,789		probably benign	Het
Depdc5	T	C	5: 32,933,937		probably benign	Het
Dgkh	A	C	14: 78,570,129	Y1149*	probably null	Het
Dnaic1	A	G	4: 41,635,162		probably benign	Het
Efcab2	A	G	1: 178,474,886	E65G	possibly damaging	Het
Eno1b	T	C	18: 48,047,739	I328T	probably benign	Het
Fam71e2	A	T	7: 4,770,287	L177Q	probably damaging	Het
Fgfr4	A	G	13: 55,161,385		probably benign	Het
Gm10720	A	C	9: 3,015,787	S44R	probably null	Het
Gm17535	T	A	9: 3,035,786	L218H	probably benign	Het
Gm6471	A	T	7: 142,831,631		noncoding transcript	Het
Gm8994	A	T	6: 136,328,844	D101V	probably damaging	Het
Hnrnpm	C	T	17: 33,646,515	R724Q	probably damaging	Het
Homer1	C	T	13: 93,391,746	T117I	possibly damaging	Het
Hoxd4	A	T	2: 74,727,457	Q60L	probably damaging	Het
Ift172	T	C	5: 31,260,624	R1274G	probably benign	Het
Ino80d	A	G	1: 63,058,318	S806P	probably damaging	Het
Itga10	T	C	3: 96,653,700	V627A	probably benign	Het
Itgb2l	A	G	16: 96,437,369	Y77H	possibly damaging	Het
Kel	A	T	6: 41,701,943	H195Q	probably benign	Het
Klhdc7a	A	G	4: 139,967,271	S122P	possibly damaging	Het
Krt71	T	A	15: 101,734,706	I456F	possibly damaging	Het
Lats1	T	A	10: 7,691,575	S37T	probably damaging	Het
Lrp1b	T	A	2: 41,123,019	H1858L	possibly damaging	Het
Matk	A	T	10: 81,262,842	T461S	probably benign	Het
Meikin	A	G	11: 54,409,642		probably benign	Het
Muc6	T	C	7: 141,634,116	Q2832R	possibly damaging	Het
Myo10	T	C	15: 25,781,238	F194L	possibly damaging	Het
Nbas	G	A	12: 13,273,876		probably benign	Het
Nme4	A	G	17: 26,093,857		probably null	Het
Olfr1136	A	G	2: 87,693,604	S93P	probably benign	Het
Olfr1217	T	A	2: 89,023,196	N269I	probably benign	Het
Olfr1340	A	T	4: 118,726,333	I29F	possibly damaging	Het
Olfr851	T	A	9: 19,496,937	L63H	probably damaging	Het
Olfr954	T	C	9: 39,461,671	V80A	probably damaging	Het
Pacsin2	T	C	15: 83,377,661	Q473R	probably benign	Het
Patz1	A	G	11: 3,293,288	H427R	probably damaging	Het
Pkp3	A	G	7: 141,083,343	Y367C	probably damaging	Het
Prdm2	G	A	4: 143,133,768	P984L	possibly damaging	Het
Rev3l	T	A	10: 39,874,128	C3091*	probably null	Het
Rims4	C	T	2: 163,863,929	V262M	possibly damaging	Het
Rpl5	T	C	5: 107,904,757	F140L	probably benign	Het
Sec24a	A	C	11: 51,700,826	I1014M	probably benign	Het
Serpinb11	A	G	1: 107,372,203	H93R	probably benign	Het
Shank2	C	A	7: 144,070,135	H286N	probably benign	Het
Sipa1l2	G	T	8: 125,421,898	Q1651K	probably damaging	Het
Slc17a3	C	T	13: 23,855,858	S293F	probably damaging	Het
Slc30a5	A	T	13: 100,826,494	F75L	possibly damaging	Het
Slco1a1	G	T	6: 141,910,701		probably benign	Het
Smg5	C	T	3: 88,353,872		probably benign	Het
Snapc1	C	T	12: 73,975,032	R81C	probably damaging	Het
Taf8	A	T	17: 47,498,252		probably benign	Het
Tarsl2	A	G	7: 65,684,081	D617G	probably damaging	Het
Tbc1d5	A	T	17: 50,984,687		probably benign	Het
Tfcp2	C	G	15: 100,514,827	E315Q	probably damaging	Het
Tmf1	A	T	6: 97,170,384	S540R	probably damaging	Het
Tmprss4	T	C	9: 45,184,336	Q70R	probably benign	Het
Trip13	G	T	13: 73,920,064	A266E	possibly damaging	Het
Ttc24	T	A	3: 88,074,927		probably benign	Het
Ttll5	T	A	12: 85,831,966	I49N	probably damaging	Het
Ube2ql1	A	T	13: 69,738,592	M250K	possibly damaging	Het
Vmn1r87	A	T	7: 13,132,284	D25E	probably damaging	Het
Vmn2r84	A	G	10: 130,392,008	Y120H	probably benign	Het
Wdr6	G	A	9: 108,575,242	R481C	probably damaging	Het
Wdr60	A	G	12: 116,232,636	V497A	probably benign	Het
Zcchc6	G	A	13: 59,782,336	R962*	probably null	Het
Zdhhc17	A	T	10: 110,955,094	Y371*	probably null	Het
Zfp292	T	C	4: 34,811,185	N620D	probably benign	Het
Zfp932	T	A	5: 110,006,968	Y11N	probably benign	Het

Other mutations in Amn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02391:Amn1	APN	6	149169446	critical splice donor site	probably null
PIT4453001:Amn1	UTSW	6	149170859	missense	probably benign	0.00
R0494:Amn1	UTSW	6	149185136	unclassified	probably benign
R0557:Amn1	UTSW	6	149171005	missense	possibly damaging	0.50
R0717:Amn1	UTSW	6	149183472	missense	possibly damaging	0.88
R0736:Amn1	UTSW	6	149183472	missense	possibly damaging	0.88
R3420:Amn1	UTSW	6	149169452	nonsense	probably null
R3421:Amn1	UTSW	6	149169452	nonsense	probably null
R4466:Amn1	UTSW	6	149166845	splice site	probably null
R4760:Amn1	UTSW	6	149185113	missense	probably benign
R5294:Amn1	UTSW	6	149185124	unclassified	probably benign
R5356:Amn1	UTSW	6	149166894	missense	possibly damaging	0.80
R5561:Amn1	UTSW	6	149185024	missense	probably damaging	0.98
R7501:Amn1	UTSW	6	149185031	missense	probably benign	0.19
R7564:Amn1	UTSW	6	149185031	missense	probably benign	0.19
R7643:Amn1	UTSW	6	149185031	missense	probably benign	0.19
R7645:Amn1	UTSW	6	149185031	missense	probably benign	0.19
R8097:Amn1	UTSW	6	149169355	unclassified	probably benign
R9390:Amn1	UTSW	6	149183485	missense	probably damaging	1.00
R9565:Amn1	UTSW	6	149188605	start gained	probably benign
X0064:Amn1	UTSW	6	149171035	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGCTGAAGAGCCTTGAACTTCAAC -3'
(R):5'- TGGATGCCTCTACATTTTGTGCCAG -3'

Sequencing Primer
(F):5'- gaactgccacgccctac -3'
(R):5'- CCAGTTAGTTCTGAGCAGCC -3'

Posted On

2013-04-16