Incidental Mutation 'R3894:Morf4l1'
ID310453
Institutional Source Beutler Lab
Gene Symbol Morf4l1
Ensembl Gene ENSMUSG00000062270
Gene Namemortality factor 4 like 1
SynonymsMRG15, Tex189, MORFRG15, TEG-189
Accession Numbers
Is this an essential gene? Essential (E-score: 1.000) question?
Stock #R3894 (G1)
Quality Score153
Status Not validated
Chromosome9
Chromosomal Location90091665-90114774 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 90094448 bp
ZygosityHeterozygous
Amino Acid Change Phenylalanine to Isoleucine at position 276 (F276I)
Ref Sequence ENSEMBL: ENSMUSP00000082346 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000085248] [ENSMUST00000169860] [ENSMUST00000190345] [ENSMUST00000191189] [ENSMUST00000191353]
Predicted Effect possibly damaging
Transcript: ENSMUST00000085248
AA Change: F276I

PolyPhen 2 Score 0.905 (Sensitivity: 0.82; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000082346
Gene: ENSMUSG00000062270
AA Change: F276I

DomainStartEndE-ValueType
Pfam:Tudor-knot 11 53 8.9e-11 PFAM
Blast:CHROMO 83 117 4e-6 BLAST
Pfam:MRG 174 348 3.5e-57 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000169860
AA Change: F237I

PolyPhen 2 Score 0.124 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000132020
Gene: ENSMUSG00000062270
AA Change: F237I

DomainStartEndE-ValueType
CHROMO 10 78 1.8e-9 SMART
Pfam:MRG 127 311 2.8e-59 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000181611
Predicted Effect noncoding transcript
Transcript: ENSMUST00000187771
Predicted Effect noncoding transcript
Transcript: ENSMUST00000188905
Predicted Effect noncoding transcript
Transcript: ENSMUST00000189089
Predicted Effect noncoding transcript
Transcript: ENSMUST00000189420
Predicted Effect probably benign
Transcript: ENSMUST00000190345
Predicted Effect noncoding transcript
Transcript: ENSMUST00000190377
Predicted Effect probably benign
Transcript: ENSMUST00000191189
AA Change: F210I

PolyPhen 2 Score 0.035 (Sensitivity: 0.94; Specificity: 0.82)
SMART Domains Protein: ENSMUSP00000140118
Gene: ENSMUSG00000062270
AA Change: F210I

DomainStartEndE-ValueType
CHROMO 10 78 1.1e-11 SMART
Pfam:MRG 100 284 1.1e-56 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000191353
SMART Domains Protein: ENSMUSP00000140023
Gene: ENSMUSG00000062270

DomainStartEndE-ValueType
Pfam:Tudor-knot 11 53 3.1e-8 PFAM
Blast:CHROMO 82 116 2e-6 BLAST
Meta Mutation Damage Score 0.5531 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.0%
Validation Efficiency
MGI Phenotype PHENOTYPE: Homozygous null mice display perinatal lethality, cardiac hypertrophy, reduced alveolar space, decreased cell proliferation, congestion of the liver, lung, and spleen, skin edema, and thin skin. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 45 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Akt1s1 A G 7: 44,853,939 D180G probably damaging Het
Aldh1a7 A T 19: 20,696,398 Y457* probably null Het
Alpk3 C T 7: 81,078,390 P423S possibly damaging Het
Aox2 A T 1: 58,334,678 probably null Het
Crebbp T C 16: 4,096,102 T1316A probably benign Het
Cul3 C A 1: 80,283,690 V273F probably damaging Het
Dnah1 G T 14: 31,307,028 R582S probably benign Het
Fbxl2 T C 9: 114,003,193 N51S probably damaging Het
Gapvd1 T C 2: 34,728,476 D295G probably benign Het
Gdf7 A G 12: 8,298,845 S151P unknown Het
Gm10277 T C 11: 77,786,001 probably benign Het
Hdac4 T C 1: 91,970,968 E688G possibly damaging Het
Htr1d A G 4: 136,443,237 E259G probably benign Het
Ifi204 T C 1: 173,749,208 H609R possibly damaging Het
Ift80 T C 3: 68,917,999 D541G probably damaging Het
Il18r1 A T 1: 40,474,874 H80L possibly damaging Het
Lrp4 G A 2: 91,473,949 G158S probably damaging Het
Mesd T A 7: 83,897,785 L152H probably damaging Het
Mslnl G A 17: 25,742,934 V128M probably damaging Het
Mut G A 17: 40,955,139 C531Y probably damaging Het
Myo15 A G 11: 60,504,319 T2480A probably benign Het
Olfr1284 C A 2: 111,379,637 F212L probably benign Het
Olfr1317 T C 2: 112,142,014 I23T probably benign Het
Olfr1387 G A 11: 49,459,939 G87R possibly damaging Het
Olfr180 A G 16: 58,916,339 F101L probably benign Het
Olfr437 A T 6: 43,167,258 I67F probably benign Het
Ovgp1 T C 3: 105,986,567 probably benign Het
Ovgp1 A G 3: 105,986,596 probably benign Het
Pcbp4 A T 9: 106,461,371 Q59L possibly damaging Het
Prg4 G C 1: 150,454,759 probably benign Het
Rad50 T A 11: 53,678,870 I905L probably benign Het
Rp1l1 T C 14: 64,029,307 S781P probably benign Het
Rps3 C T 7: 99,479,896 R173H probably benign Het
Rtn3 T A 19: 7,435,085 T86S probably damaging Het
Sdha A T 13: 74,334,391 S268T probably benign Het
Sgo2b A T 8: 63,928,733 V355E possibly damaging Het
Sh3glb2 T C 2: 30,355,288 T60A probably damaging Het
Slc26a3 A C 12: 31,464,720 Y513S probably damaging Het
Slc35b2 T C 17: 45,566,442 V165A probably benign Het
Slco3a1 A G 7: 74,284,613 W604R probably damaging Het
Tet2 A G 3: 133,469,477 S1370P possibly damaging Het
Tmtc4 A T 14: 122,921,319 probably null Het
Tsga13 A G 6: 30,912,263 V18A probably benign Het
Ugt2a3 T C 5: 87,329,590 T317A probably benign Het
Zcchc4 A T 5: 52,784,100 D79V probably damaging Het
Other mutations in Morf4l1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02145:Morf4l1 APN 9 90093795 missense probably benign 0.16
IGL03309:Morf4l1 APN 9 90103745 missense probably benign
R0848:Morf4l1 UTSW 9 90100449 missense probably benign 0.24
R0893:Morf4l1 UTSW 9 90102350 missense probably damaging 1.00
R1155:Morf4l1 UTSW 9 90094504 missense probably benign 0.11
R1765:Morf4l1 UTSW 9 90102348 missense possibly damaging 0.60
R1972:Morf4l1 UTSW 9 90095214 unclassified probably benign
R3805:Morf4l1 UTSW 9 90095143 missense probably benign 0.10
R3806:Morf4l1 UTSW 9 90095143 missense probably benign 0.10
R3895:Morf4l1 UTSW 9 90094448 missense possibly damaging 0.90
R5460:Morf4l1 UTSW 9 90095130 missense probably benign 0.10
R6884:Morf4l1 UTSW 9 90094479 missense probably damaging 1.00
R7088:Morf4l1 UTSW 9 90097380 missense possibly damaging 0.59
R7869:Morf4l1 UTSW 9 90093791 missense probably damaging 1.00
R7876:Morf4l1 UTSW 9 90093806 missense possibly damaging 0.65
R7952:Morf4l1 UTSW 9 90093791 missense probably damaging 1.00
R7959:Morf4l1 UTSW 9 90093806 missense possibly damaging 0.65
Predicted Primers
Posted On2015-04-17