Mutagenetix > Incidental Mutation

Incidental Mutation 'R5029:Dact2'

391587

Institutional Source

Beutler Lab

Gene Symbol

Dact2

Ensembl Gene

ENSMUSG00000048826

Gene Name

dishevelled-binding antagonist of beta-catenin 2

Synonyms

dapper2, Dpr2, Frd2, 2900084M21Rik

MMRRC Submission

042620-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5029 (G1)

Quality Score

178

Status

Validated

Chromosome

Chromosomal Location

14195231-14203831 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 14195852 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Arginine at position 695 (S695R)

Ref Sequence

ENSEMBL: ENSMUSP00000051638 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053218]

AlphaFold

Q7TN08

Predicted Effect

probably benign
Transcript: ENSMUST00000053218
AA Change: S695R

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000051638
Gene: ENSMUSG00000048826
AA Change: S695R

Domain	Start	End	E-Value	Type
Pfam:Dapper	19	757	1.8e-231	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.6%
20x: 93.1%

Validation Efficiency

97% (61/63)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele develop normally but display enhanced keratinocyte migration and accelerated re-epithelialization during cutaneous wound healing. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actbl2	T	A	13: 111,255,593	M154K	probably benign	Het
Ank	T	C	15: 27,590,353	V341A	probably benign	Het
Bnip3	T	A	7: 138,898,119		probably benign	Het
C1galt1	A	G	6: 7,863,931	K4E	possibly damaging	Het
Cbs	T	A	17: 31,615,482	Q511L	possibly damaging	Het
Ccdc117	T	C	11: 5,534,897	H109R	possibly damaging	Het
Cdh4	T	A	2: 179,881,949	V440D	possibly damaging	Het
Cnot4	T	C	6: 35,078,027	N109S	probably damaging	Het
Defa21	A	T	8: 21,026,365	E75V	probably damaging	Het
Dimt1	A	G	13: 106,957,122	D274G	probably null	Het
Dstyk	G	T	1: 132,449,324	V223L	probably benign	Het
Dync1h1	T	A	12: 110,618,010	S616T	possibly damaging	Het
Fer1l6	A	T	15: 58,643,920	I1619F	probably benign	Het
Gm4744	T	A	6: 40,949,407	Y83F	probably null	Het
Gm4787	G	C	12: 81,377,830	T518S	probably benign	Het
Hpx	T	G	7: 105,591,764	Y434S	probably damaging	Het
Ints4	A	G	7: 97,509,774	D451G	probably benign	Het
Itgb4	A	T	11: 115,988,591		probably benign	Het
Jph1	T	A	1: 17,091,391	N349I	possibly damaging	Het
Klrg1	A	G	6: 122,282,735	L26P	probably benign	Het
M1ap	A	C	6: 83,003,832	D233A	probably damaging	Het
Mfng	C	T	15: 78,764,388	R163H	probably benign	Het
Mycbp2	A	G	14: 103,156,510	S2762P	probably benign	Het
Myh11	T	A	16: 14,205,625	Q1556L	probably damaging	Het
N4bp2	A	G	5: 65,814,780	D1428G	probably damaging	Het
Ndc80	A	C	17: 71,508,765	C342G	probably benign	Het
Nxn	A	C	11: 76,274,530	Y199*	probably null	Het
Olfr1057	A	G	2: 86,374,890	I174T	probably damaging	Het
Olfr371	C	T	8: 85,231,206	A237V	probably benign	Het
Olfr776	T	A	10: 129,261,838	N292K	probably benign	Het
Pacs1	A	G	19: 5,142,271	F691L	probably benign	Het
Pcdhga12	T	C	18: 37,766,811	V232A	probably benign	Het
Pik3cb	A	T	9: 99,054,060	M773K	probably damaging	Het
Plxnb1	T	C	9: 109,114,655	Y1937H	probably damaging	Het
Ppfia2	C	A	10: 106,857,443	T603N	probably benign	Het
Psg22	A	G	7: 18,719,737	Y119C	probably damaging	Het
Saraf	G	T	8: 34,161,258	R86L	probably damaging	Het
Sh3bgrl2	T	C	9: 83,548,489	S11P	possibly damaging	Het
Slc39a4	C	T	15: 76,614,083	D385N	probably damaging	Het
St13	G	C	15: 81,399,585	R4G	probably benign	Het
Stpg3	T	C	2: 25,214,564	N13S	probably damaging	Het
Taf1a	T	A	1: 183,395,935	D50E	possibly damaging	Het
Tlr1	T	A	5: 64,925,681	N518Y	probably damaging	Het
Tnik	G	A	3: 28,665,844		probably null	Het
Tomm70a	T	A	16: 57,122,151	L97Q	probably benign	Het
Vmn2r97	T	A	17: 18,947,911	V809E	probably damaging	Het
Vps13d	T	C	4: 145,156,282	I914V	probably benign	Het
Zfyve26	A	T	12: 79,286,323	H291Q	probably damaging	Het
Zmynd15	G	T	11: 70,462,561	C334F	probably damaging	Het

Other mutations in Dact2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01867:Dact2	APN	17	14195670	missense	probably damaging	0.98
R0597:Dact2	UTSW	17	14197041	missense	probably benign
R1657:Dact2	UTSW	17	14197990	missense	probably benign	0.03
R1717:Dact2	UTSW	17	14197913	missense	probably benign	0.11
R1734:Dact2	UTSW	17	14196639	missense	probably benign	0.00
R1883:Dact2	UTSW	17	14197823	missense	possibly damaging	0.81
R2238:Dact2	UTSW	17	14197050	missense	probably damaging	0.99
R2878:Dact2	UTSW	17	14195914	missense	probably damaging	1.00
R4290:Dact2	UTSW	17	14196571	missense	probably benign	0.00
R4291:Dact2	UTSW	17	14196571	missense	probably benign	0.00
R4902:Dact2	UTSW	17	14196729	missense	possibly damaging	0.91
R5084:Dact2	UTSW	17	14197952	missense	possibly damaging	0.94
R5436:Dact2	UTSW	17	14195748	missense	probably damaging	1.00
R5837:Dact2	UTSW	17	14196253	missense	probably damaging	1.00
R5847:Dact2	UTSW	17	14199188	missense	probably damaging	0.97
R6048:Dact2	UTSW	17	14197305	missense	probably damaging	1.00
R6377:Dact2	UTSW	17	14199188	missense	probably damaging	0.97
R7013:Dact2	UTSW	17	14203534	missense	probably benign
R7268:Dact2	UTSW	17	14196535	missense	probably benign	0.01
R7425:Dact2	UTSW	17	14196331	missense	probably damaging	1.00
R8725:Dact2	UTSW	17	14196884	nonsense	probably null
R8727:Dact2	UTSW	17	14196884	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GCCATCACACCATGGTCATG -3'
(R):5'- ATGGAGATCTCAGCCAAAGCC -3'

Sequencing Primer
(F):5'- TCATGACCTTCAGGGCTGCTG -3'
(R):5'- AAAGCCGGTTCAGTCAGC -3'

Posted On

2016-06-06