|Institutional Source||Beutler Lab|
|Gene Name||eukaryotic translation initiation factor 5B|
|Is this an essential gene?||Essential (E-score: 1.000)|
|Stock #||R6877 (G1)|
|Chromosomal Location||37998010-38055579 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 38050239 bp|
|Amino Acid Change||Asparagine to Tyrosine at position 1099 (N1099Y)|
|Ref Sequence||ENSEMBL: ENSMUSP00000027252 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000027251] [ENSMUST00000027252]|
|Predicted Effect||probably benign
|Predicted Effect||probably damaging
AA Change: N1099Y
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: N1099Y
|Meta Mutation Damage Score||0.6616|
|Coding Region Coverage||
|Validation Efficiency||94% (47/50)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Eif5b||
(F):5'- TGGGGCATGAACATGTTCCC -3'
(R):5'- TTAGGAATGCAGACGGACATTAC -3'
(F):5'- GCATGAACATGTTCCCTGATGTAGC -3'
(R):5'- CATTACTGAAGTGATACCACCTAGGG -3'