|Institutional Source||Beutler Lab|
|Gene Name||keratin 15|
|Is this an essential gene?||Probably non essential (E-score: 0.086)|
|Stock #||R7389 (G1)|
|Chromosomal Location||100131758-100135928 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 100135560 bp|
|Amino Acid Change||Valine to Glutamic Acid at position 100 (V100E)|
|Ref Sequence||ENSEMBL: ENSMUSP00000103034 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000107411]|
|Predicted Effect||possibly damaging
AA Change: V100E
PolyPhen 2 Score 0.792 (Sensitivity: 0.85; Specificity: 0.93)
AA Change: V100E
|Meta Mutation Damage Score||0.1795|
|Coding Region Coverage||
|Validation Efficiency||98% (49/50)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit impaired esophageal epithelial regeneration with thicker epithelia due to enhanced basal cell proliferation. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Krt15||
(F):5'- TGTACTGAACACGAGCCACC -3'
(R):5'- GAAGCCGAAGTATCTCTGCTTCTTC -3'
(F):5'- GACAGACTTACTTTGTCCCGG -3'
(R):5'- GAAGTATCTCTGCTTCTTCTGCTAGG -3'