Mutagenetix > Incidental Mutation

Incidental Mutation 'R7724:Or8s5'

595419

Institutional Source

Beutler Lab

Gene Symbol

Or8s5

Ensembl Gene

ENSMUSG00000051793

Gene Name

olfactory receptor family 8 subfamily S member 5

Synonyms

Olfr284, MOR160-4, GA_x6K02T2NBG7-5395976-5396893

MMRRC Submission

045780-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R7724 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

98237903-98238820 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 98238775 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 32 (S32T)

Ref Sequence

ENSEMBL: ENSMUSP00000145864 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000063289] [ENSMUST00000206647]

AlphaFold

A0A0U1RP76

Predicted Effect

possibly damaging
Transcript: ENSMUST00000063289
AA Change: S16T

PolyPhen 2 Score 0.894 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000065626
Gene: ENSMUSG00000051793
AA Change: S16T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	304	5.3e-54	PFAM
Pfam:7TM_GPCR_Srsx	33	259	1.3e-5	PFAM
Pfam:7tm_1	39	286	5e-21	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000206647
AA Change: S32T

PolyPhen 2 Score 0.894 (Sensitivity: 0.82; Specificity: 0.94)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130401M01Rik	A	C	15: 57,885,857 (GRCm39)	S342A	probably benign	Het
Adarb2	C	A	13: 8,620,292 (GRCm39)	H259Q	probably benign	Het
Ak7	T	A	12: 105,682,289 (GRCm39)	I150N	probably damaging	Het
Arhgef12	A	T	9: 42,938,567 (GRCm39)	I75N	probably damaging	Het
Arhgef17	C	T	7: 100,529,816 (GRCm39)	G480S	probably damaging	Het
Atic	T	C	1: 71,604,060 (GRCm39)	Y208H	probably damaging	Het
Atp8b4	T	A	2: 126,164,813 (GRCm39)	N1168Y	possibly damaging	Het
Ccdc202	A	G	14: 96,119,686 (GRCm39)	N148D	possibly damaging	Het
Cdh15	G	T	8: 123,593,700 (GRCm39)	D765Y	probably damaging	Het
Celf4	C	T	18: 25,619,850 (GRCm39)		probably null	Het
Cep57l1	G	A	10: 41,621,838 (GRCm39)	T26M	possibly damaging	Het
Ces2g	T	A	8: 105,693,484 (GRCm39)	D388E	probably benign	Het
Cir1	T	C	2: 73,137,234 (GRCm39)	N69S	possibly damaging	Het
Ctc1	C	A	11: 68,917,170 (GRCm39)	H355N	probably benign	Het
Ebf2	A	T	14: 67,661,489 (GRCm39)	K539M	probably damaging	Het
Ehbp1	G	A	11: 22,039,572 (GRCm39)	H843Y	probably null	Het
Fat2	C	T	11: 55,175,622 (GRCm39)	G1697E	probably damaging	Het
Fga	A	G	3: 82,936,432 (GRCm39)	D76G	probably damaging	Het
Fn1	A	T	1: 71,642,894 (GRCm39)	S1786T	probably benign	Het
Frmpd1	A	T	4: 45,229,888 (GRCm39)	E18D	probably damaging	Het
Gbp8	A	T	5: 105,179,160 (GRCm39)	I113N	probably damaging	Het
Gm10549	A	T	18: 33,603,912 (GRCm39)	R132*	probably null	Het
Gmps	T	A	3: 63,893,074 (GRCm39)	D126E	possibly damaging	Het
Gria4	A	G	9: 4,472,074 (GRCm39)	Y472H	probably damaging	Het
Helz2	T	C	2: 180,873,789 (GRCm39)	Y2235C	probably damaging	Het
Hrnr	A	G	3: 93,230,323 (GRCm39)	N187S	unknown	Het
Ifit1bl1	T	C	19: 34,571,405 (GRCm39)	K351E	probably benign	Het
Klhl23	A	G	2: 69,655,056 (GRCm39)	T309A	probably benign	Het
Ldhal6b	A	G	17: 5,468,877 (GRCm39)	V19A	probably benign	Het
Lrrc59	C	T	11: 94,534,170 (GRCm39)	R243C	probably damaging	Het
Magi3	A	C	3: 103,923,243 (GRCm39)	I1158R	probably benign	Het
Mical2	T	A	7: 111,922,833 (GRCm39)	Y613N	probably damaging	Het
Myoc	T	A	1: 162,467,396 (GRCm39)		probably null	Het
Npas3	C	A	12: 54,115,124 (GRCm39)	S682Y	possibly damaging	Het
Ogdh	T	C	11: 6,274,887 (GRCm39)	V172A	probably benign	Het
Or2t46	T	A	11: 58,472,208 (GRCm39)	H179Q	probably benign	Het
Or52r1c	T	A	7: 102,735,470 (GRCm39)	C243*	probably null	Het
Or56a42-ps1	A	G	7: 104,777,459 (GRCm39)	Y52H	probably damaging	Het
Or8k30	A	G	2: 86,338,949 (GRCm39)	T49A	probably damaging	Het
P2rx7	A	T	5: 122,811,436 (GRCm39)	I331F	probably benign	Het
Pcdhb14	A	T	18: 37,581,937 (GRCm39)	T348S	possibly damaging	Het
Prdx6b	A	G	2: 80,123,746 (GRCm39)	E185G	probably benign	Het
Rdh1	A	G	10: 127,600,576 (GRCm39)	K200E	possibly damaging	Het
Sema6c	A	T	3: 95,080,510 (GRCm39)	R935W	probably damaging	Het
Serpinb5	A	T	1: 106,802,872 (GRCm39)	I136F	probably damaging	Het
Skint10	A	T	4: 112,622,289 (GRCm39)	Y31*	probably null	Het
St6galnac1	C	T	11: 116,656,898 (GRCm39)		probably null	Het
Synj1	G	A	16: 90,758,387 (GRCm39)	T819M	probably damaging	Het
Tacr3	T	A	3: 134,635,669 (GRCm39)	V291E	probably damaging	Het
Tdo2	A	G	3: 81,875,390 (GRCm39)		probably null	Het
Tex15	T	A	8: 34,036,291 (GRCm39)	D72E	possibly damaging	Het
Tph2	T	C	10: 114,915,727 (GRCm39)	T438A	probably benign	Het
Uaca	A	G	9: 60,777,187 (GRCm39)	T525A	probably benign	Het
Ulk1	A	T	5: 110,940,270 (GRCm39)	C384S	probably benign	Het
Uvrag	T	C	7: 98,641,170 (GRCm39)	D290G	probably benign	Het
Zfp541	T	C	7: 15,805,919 (GRCm39)	L18P	probably damaging	Het

Other mutations in Or8s5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Or8s5	APN	15	98,238,054 (GRCm39)	missense	possibly damaging	0.47
IGL02904:Or8s5	APN	15	98,238,729 (GRCm39)	missense	probably null	0.88
PIT4378001:Or8s5	UTSW	15	98,238,153 (GRCm39)	missense	possibly damaging	0.95
R0485:Or8s5	UTSW	15	98,238,810 (GRCm39)	missense	probably benign	0.01
R1435:Or8s5	UTSW	15	98,238,209 (GRCm39)	missense	possibly damaging	0.69
R4706:Or8s5	UTSW	15	98,238,659 (GRCm39)	missense	possibly damaging	0.62
R4707:Or8s5	UTSW	15	98,238,659 (GRCm39)	missense	possibly damaging	0.62
R5272:Or8s5	UTSW	15	98,238,246 (GRCm39)	missense	probably benign	0.00
R5314:Or8s5	UTSW	15	98,238,246 (GRCm39)	missense	probably benign	0.00
R5315:Or8s5	UTSW	15	98,238,246 (GRCm39)	missense	probably benign	0.00
R5316:Or8s5	UTSW	15	98,238,246 (GRCm39)	missense	probably benign	0.00
R5317:Or8s5	UTSW	15	98,238,246 (GRCm39)	missense	probably benign	0.00
R5456:Or8s5	UTSW	15	98,238,246 (GRCm39)	missense	probably benign	0.00
R5458:Or8s5	UTSW	15	98,238,246 (GRCm39)	missense	probably benign	0.00
R5548:Or8s5	UTSW	15	98,238,253 (GRCm39)	missense	probably benign	0.21
R5717:Or8s5	UTSW	15	98,238,246 (GRCm39)	missense	probably benign	0.00
R5921:Or8s5	UTSW	15	98,238,310 (GRCm39)	missense	probably benign	0.10
R6519:Or8s5	UTSW	15	98,237,929 (GRCm39)	missense	probably benign	0.00
R6636:Or8s5	UTSW	15	98,238,831 (GRCm39)	missense	probably benign	0.23
R7112:Or8s5	UTSW	15	98,238,421 (GRCm39)	missense	possibly damaging	0.81
R7289:Or8s5	UTSW	15	98,237,943 (GRCm39)	missense	probably damaging	1.00
R7392:Or8s5	UTSW	15	98,238,192 (GRCm39)	missense	probably benign	0.03
R7403:Or8s5	UTSW	15	98,238,000 (GRCm39)	missense	probably damaging	1.00
R7633:Or8s5	UTSW	15	98,237,967 (GRCm39)	missense	probably damaging	1.00
R9451:Or8s5	UTSW	15	98,238,144 (GRCm39)	missense	possibly damaging	0.61
R9707:Or8s5	UTSW	15	98,238,154 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GATTCTCGAGCATCTTGGGC -3'
(R):5'- TAATGGTCTTAGTCCCGGCCAG -3'

Sequencing Primer
(F):5'- ATCTTGGGCGCCGTGACAG -3'
(R):5'- CCAGGGAGTTCATTTTTAAGTAGC -3'

Posted On

2019-11-12