Mutagenetix > Incidental Mutation

Incidental Mutation 'R7850:Tas2r135'

606879

Institutional Source

Beutler Lab

Gene Symbol

Tas2r135

Ensembl Gene

ENSMUSG00000056203

Gene Name

taste receptor, type 2, member 135

Synonyms

mt2r38, Tas2r35

MMRRC Submission

045903-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.051) question?

Stock #

R7850 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

42382463-42383428 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 42383072 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 204 (F204I)

Ref Sequence

ENSEMBL: ENSMUSP00000070247 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000070178]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000070178
AA Change: F204I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000070247
Gene: ENSMUSG00000056203
AA Change: F204I

Domain	Start	End	E-Value	Type
Pfam:TAS2R	22	320	1.3e-63	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (43/43)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A630089N07Rik	A	T	16: 97,869,328 (GRCm39)	D2E	probably damaging	Het
Abhd16b	G	A	2: 181,135,518 (GRCm39)	R140H	not run	Het
Acad11	G	T	9: 103,991,728 (GRCm39)	G588C	probably damaging	Het
Baz2b	C	A	2: 59,767,060 (GRCm39)	E107D	probably damaging	Het
Bcas1	A	G	2: 170,190,023 (GRCm39)	S625P	probably damaging	Het
Bdp1	T	A	13: 100,228,832 (GRCm39)	L265F	probably damaging	Het
Cacna1s	A	G	1: 135,998,786 (GRCm39)	Y227C	probably damaging	Het
Cacna2d2	G	A	9: 107,402,575 (GRCm39)	A848T	probably benign	Het
Cep135	T	C	5: 76,739,720 (GRCm39)		probably null	Het
Cyp2b9	G	T	7: 25,886,111 (GRCm39)	E93*	probably null	Het
Dcp2	C	A	18: 44,533,415 (GRCm39)	S122*	probably null	Het
Ecel1	A	G	1: 87,079,745 (GRCm39)	L456P	probably damaging	Het
Entpd8	T	C	2: 24,975,028 (GRCm39)	M453T	probably damaging	Het
Gcc2	T	A	10: 58,114,703 (GRCm39)	V1084D	probably damaging	Het
Grem1	A	G	2: 113,580,178 (GRCm39)	C108R	probably damaging	Het
Gtf2ird1	T	C	5: 134,392,069 (GRCm39)	E880G	probably benign	Het
Hacd2	T	A	16: 34,922,477 (GRCm39)	M222K	probably damaging	Het
Htr1b	G	T	9: 81,514,652 (GRCm39)		probably null	Het
Htt	T	G	5: 35,009,631 (GRCm39)		probably null	Het
Ifnab	T	G	4: 88,609,133 (GRCm39)	D111A	probably benign	Het
Igsf10	A	T	3: 59,227,053 (GRCm39)	L2207I	probably benign	Het
Itln1	T	C	1: 171,358,166 (GRCm39)	Y194C	probably damaging	Het
Kmt5b	T	A	19: 3,865,043 (GRCm39)	D702E	probably damaging	Het
Knop1	T	C	7: 118,449,860 (GRCm39)	E382G	unknown	Het
Lrit3	A	C	3: 129,594,452 (GRCm39)	L42V	probably damaging	Het
Mug2	A	G	6: 122,052,170 (GRCm39)	E976G	probably damaging	Het
Or5an10	T	C	19: 12,275,996 (GRCm39)	M167V	probably benign	Het
Or8g28	A	T	9: 39,169,518 (GRCm39)	I150N	possibly damaging	Het
Or8s8	G	A	15: 98,354,949 (GRCm39)	G253R	probably damaging	Het
Papln	A	T	12: 83,827,436 (GRCm39)	E758V	probably damaging	Het
Pcnx3	A	C	19: 5,728,960 (GRCm39)	F967V	possibly damaging	Het
Pcnx4	G	T	12: 72,602,842 (GRCm39)	C368F	probably benign	Het
Pi15	T	A	1: 17,673,105 (GRCm39)	Y101*	probably null	Het
Ppp1r9a	G	A	6: 4,905,894 (GRCm39)	V150M	possibly damaging	Het
Prrt4	A	T	6: 29,176,900 (GRCm39)		probably null	Het
Ptgdr	A	G	14: 45,090,828 (GRCm39)	V310A	probably benign	Het
Ric1	G	A	19: 29,572,293 (GRCm39)	S696N	probably benign	Het
Setx	T	A	2: 29,037,430 (GRCm39)	V1305D	probably damaging	Het
Slk	G	A	19: 47,610,796 (GRCm39)	R822H	probably damaging	Het
Tcaf3	A	T	6: 42,571,140 (GRCm39)		probably null	Het
Tmtc2	A	G	10: 105,409,568 (GRCm39)	Y15H	probably benign	Het
Treml2	T	C	17: 48,615,168 (GRCm39)	S218P	probably benign	Het
Tubb2a	T	C	13: 34,258,554 (GRCm39)	E412G	probably damaging	Het
Usp48	T	G	4: 137,333,060 (GRCm39)		probably null	Het
Vmn2r25	A	T	6: 123,805,431 (GRCm39)	N475K	probably damaging	Het
Zscan4-ps1	G	A	7: 10,799,735 (GRCm39)	H385Y	probably benign	Het

Other mutations in Tas2r135

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01357:Tas2r135	APN	6	42,383,078 (GRCm39)	missense	probably benign	0.00
IGL01395:Tas2r135	APN	6	42,382,846 (GRCm39)	nonsense	probably null
IGL02479:Tas2r135	APN	6	42,382,685 (GRCm39)	nonsense	probably null
IGL02526:Tas2r135	APN	6	42,383,214 (GRCm39)	missense	probably damaging	1.00
IGL02806:Tas2r135	APN	6	42,383,382 (GRCm39)	missense	probably benign	0.00
IGL02982:Tas2r135	APN	6	42,383,187 (GRCm39)	missense	probably benign
IGL03057:Tas2r135	APN	6	42,378,061 (GRCm39)	unclassified	probably benign
R0057:Tas2r135	UTSW	6	42,383,354 (GRCm39)	missense	probably benign	0.07
R0104:Tas2r135	UTSW	6	42,383,258 (GRCm39)	missense	possibly damaging	0.79
R1412:Tas2r135	UTSW	6	42,382,768 (GRCm39)	missense	probably benign	0.00
R4517:Tas2r135	UTSW	6	42,383,013 (GRCm39)	missense	probably benign
R4629:Tas2r135	UTSW	6	42,383,160 (GRCm39)	missense	probably benign	0.03
R5788:Tas2r135	UTSW	6	42,382,531 (GRCm39)	missense	probably damaging	1.00
R6021:Tas2r135	UTSW	6	42,383,321 (GRCm39)	missense	probably damaging	1.00
R6586:Tas2r135	UTSW	6	42,382,952 (GRCm39)	missense	probably benign	0.18
R7180:Tas2r135	UTSW	6	42,382,685 (GRCm39)	nonsense	probably null
R7458:Tas2r135	UTSW	6	42,382,881 (GRCm39)	missense	possibly damaging	0.95
R9113:Tas2r135	UTSW	6	42,383,315 (GRCm39)	missense	probably benign	0.00
Z1176:Tas2r135	UTSW	6	42,383,168 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- TCTGGCTAAAGTACAGGTTGCC -3'
(R):5'- TACCAGCGTCAGAAAACAGG -3'

Sequencing Primer
(F):5'- AGGTTGCCTGGGTGGGTAC -3'
(R):5'- TACCAGCGTCAGAAAACAGGAGATG -3'

Posted On

2019-12-20