Incidental Mutation 'R8151:Zfp777'
| ID |
632950 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp777
|
| Ensembl Gene |
ENSMUSG00000071477 |
| Gene Name |
zinc finger protein 777 |
| Synonyms |
2500002G23Rik |
| MMRRC Submission |
067577-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably essential
(E-score: 0.837)
|
| Stock # |
R8151 (G1)
|
| Quality Score |
122.008 |
|
Status
|
Not validated
|
| Chromosome |
6 |
| Chromosomal Location |
48001122-48025845 bp(-) (GRCm39) |
| Type of Mutation |
nonsense |
| DNA Base Change (assembly) |
G to A
at 48006075 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Glutamine to Stop codon
at position 440
(Q440*)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000093637
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000095944]
[ENSMUST00000114583]
|
| AlphaFold |
B9EKF4 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000095944
AA Change: Q440*
|
| SMART Domains |
Protein: ENSMUSP00000093637
Gene: ENSMUSG00000071477
AA Change: Q440*
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:DUF3669
|
177 |
256 |
4.3e-12 |
PFAM |
|
KRAB
|
284 |
344 |
1.6e-29 |
SMART |
|
low complexity region
|
422 |
433 |
N/A |
INTRINSIC |
|
coiled coil region
|
454 |
477 |
N/A |
INTRINSIC |
|
low complexity region
|
489 |
505 |
N/A |
INTRINSIC |
|
low complexity region
|
520 |
532 |
N/A |
INTRINSIC |
|
low complexity region
|
539 |
555 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
557 |
579 |
1.2e-5 |
SMART |
|
ZnF_C2H2
|
585 |
607 |
3.8e-5 |
SMART |
|
ZnF_C2H2
|
646 |
668 |
1.1e-6 |
SMART |
|
ZnF_C2H2
|
674 |
696 |
4.6e-6 |
SMART |
|
ZnF_C2H2
|
704 |
726 |
3.2e-7 |
SMART |
|
ZnF_C2H2
|
732 |
754 |
3.3e-6 |
SMART |
|
ZnF_C2H2
|
760 |
782 |
8.4e-6 |
SMART |
|
ZnF_C2H2
|
788 |
810 |
4.9e-5 |
SMART |
|
ZnF_C2H2
|
816 |
838 |
1.1e-4 |
SMART |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000114583
AA Change: Q484*
|
| SMART Domains |
Protein: ENSMUSP00000110230
Gene: ENSMUSG00000071477
AA Change: Q484*
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
2 |
24 |
N/A |
INTRINSIC |
|
Pfam:DUF3669
|
231 |
298 |
4.7e-12 |
PFAM |
|
KRAB
|
328 |
388 |
3.96e-27 |
SMART |
|
low complexity region
|
466 |
477 |
N/A |
INTRINSIC |
|
coiled coil region
|
498 |
521 |
N/A |
INTRINSIC |
|
low complexity region
|
533 |
549 |
N/A |
INTRINSIC |
|
low complexity region
|
564 |
576 |
N/A |
INTRINSIC |
|
low complexity region
|
583 |
599 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
601 |
623 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
629 |
651 |
8.94e-3 |
SMART |
|
ZnF_C2H2
|
690 |
712 |
2.43e-4 |
SMART |
|
ZnF_C2H2
|
718 |
740 |
1.12e-3 |
SMART |
|
ZnF_C2H2
|
748 |
770 |
7.49e-5 |
SMART |
|
ZnF_C2H2
|
776 |
798 |
7.9e-4 |
SMART |
|
ZnF_C2H2
|
804 |
826 |
1.95e-3 |
SMART |
|
ZnF_C2H2
|
832 |
854 |
1.18e-2 |
SMART |
|
ZnF_C2H2
|
860 |
882 |
2.53e-2 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000148362
|
| SMART Domains |
Protein: ENSMUSP00000116303
Gene: ENSMUSG00000071477
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:DUF3669
|
40 |
107 |
6.1e-13 |
PFAM |
|
KRAB
|
137 |
197 |
3.96e-27 |
SMART |
|
| Coding Region Coverage |
- 1x: 99.8%
- 3x: 99.6%
- 10x: 98.5%
- 20x: 93.6%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 46 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Ahnak |
T |
C |
19: 8,982,043 (GRCm39) |
I1109T |
possibly damaging |
Het |
| Aipl1 |
C |
A |
11: 71,927,584 (GRCm39) |
D44Y |
probably benign |
Het |
| Aldh8a1 |
C |
T |
10: 21,271,465 (GRCm39) |
T397M |
probably damaging |
Het |
| Btbd16 |
T |
C |
7: 130,398,825 (GRCm39) |
S278P |
probably damaging |
Het |
| Ccdc110 |
G |
A |
8: 46,395,830 (GRCm39) |
E574K |
probably damaging |
Het |
| Cd19 |
T |
A |
7: 126,013,478 (GRCm39) |
K104* |
probably null |
Het |
| Cenpe |
A |
G |
3: 134,952,783 (GRCm39) |
E1491G |
probably benign |
Het |
| Col12a1 |
A |
T |
9: 79,537,831 (GRCm39) |
S2546T |
possibly damaging |
Het |
| Col18a1 |
T |
C |
10: 76,948,418 (GRCm39) |
T365A |
unknown |
Het |
| Ctif |
G |
T |
18: 75,653,176 (GRCm39) |
D360E |
probably benign |
Het |
| Fat4 |
G |
A |
3: 38,946,203 (GRCm39) |
E1699K |
probably damaging |
Het |
| Fbxo39 |
G |
A |
11: 72,208,526 (GRCm39) |
V293M |
probably damaging |
Het |
| Fcgbpl1 |
T |
C |
7: 27,852,766 (GRCm39) |
I1351T |
possibly damaging |
Het |
| Fhip1a |
A |
T |
3: 85,595,847 (GRCm39) |
I346N |
probably damaging |
Het |
| Havcr2 |
A |
G |
11: 46,366,722 (GRCm39) |
K221E |
possibly damaging |
Het |
| Hdac5 |
T |
C |
11: 102,097,294 (GRCm39) |
T209A |
probably benign |
Het |
| Herc1 |
A |
G |
9: 66,341,073 (GRCm39) |
Q1730R |
probably damaging |
Het |
| Ifi202b |
T |
C |
1: 173,804,923 (GRCm39) |
T10A |
probably benign |
Het |
| Il18rap |
T |
C |
1: 40,564,428 (GRCm39) |
S153P |
probably benign |
Het |
| Klk1b21 |
C |
T |
7: 43,753,787 (GRCm39) |
R24* |
probably null |
Het |
| Kras |
ACTTCTTCTTCTTCTTCTTC |
ACTTCTTCTTCTTCTTC |
6: 145,166,360 (GRCm39) |
|
probably benign |
Het |
| Ldlrad4 |
A |
G |
18: 68,383,643 (GRCm39) |
E113G |
possibly damaging |
Het |
| Lhcgr |
AT |
ATT |
17: 89,049,677 (GRCm39) |
615 |
probably null |
Het |
| Macf1 |
T |
C |
4: 123,291,206 (GRCm39) |
E3895G |
possibly damaging |
Het |
| Mug1 |
T |
C |
6: 121,818,117 (GRCm39) |
S143P |
probably benign |
Het |
| Nudcd2 |
A |
T |
11: 40,624,529 (GRCm39) |
|
probably benign |
Het |
| Nup85 |
A |
G |
11: 115,468,759 (GRCm39) |
T201A |
probably benign |
Het |
| Odad2 |
A |
G |
18: 7,127,358 (GRCm39) |
F952L |
probably damaging |
Het |
| Plppr2 |
A |
G |
9: 21,852,105 (GRCm39) |
E64G |
probably damaging |
Het |
| Plvap |
A |
G |
8: 71,960,625 (GRCm39) |
S264P |
probably benign |
Het |
| Polm |
T |
C |
11: 5,787,906 (GRCm39) |
|
probably benign |
Het |
| Polr2d |
T |
A |
18: 31,928,365 (GRCm39) |
H93Q |
probably damaging |
Het |
| Ptprt |
T |
C |
2: 162,120,005 (GRCm39) |
E154G |
probably damaging |
Het |
| Rasal2 |
C |
A |
1: 157,071,154 (GRCm39) |
G67C |
probably damaging |
Het |
| Sdk2 |
A |
G |
11: 113,763,683 (GRCm39) |
V329A |
possibly damaging |
Het |
| Sorl1 |
A |
G |
9: 41,979,229 (GRCm39) |
V423A |
probably damaging |
Het |
| Spef2 |
A |
G |
15: 9,601,598 (GRCm39) |
S1555P |
unknown |
Het |
| Srgap3 |
A |
C |
6: 112,793,628 (GRCm39) |
L116R |
probably damaging |
Het |
| St6galnac3 |
C |
T |
3: 153,117,217 (GRCm39) |
V169M |
probably damaging |
Het |
| Stx16 |
T |
A |
2: 173,935,284 (GRCm39) |
M206K |
possibly damaging |
Het |
| Txnip |
A |
G |
3: 96,466,929 (GRCm39) |
D201G |
possibly damaging |
Het |
| Ubr4 |
T |
C |
4: 139,130,112 (GRCm39) |
V718A |
probably damaging |
Het |
| Vav3 |
A |
C |
3: 109,416,164 (GRCm39) |
D261A |
probably benign |
Het |
| Vcam1 |
A |
C |
3: 115,918,128 (GRCm39) |
L278V |
possibly damaging |
Het |
| Vta1 |
C |
A |
10: 14,543,697 (GRCm39) |
A226S |
probably damaging |
Het |
| Zfp217 |
G |
A |
2: 169,961,571 (GRCm39) |
S252F |
possibly damaging |
Het |
|
| Other mutations in Zfp777 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01530:Zfp777
|
APN |
6 |
48,020,918 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01916:Zfp777
|
APN |
6 |
48,002,276 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01959:Zfp777
|
APN |
6 |
48,021,275 (GRCm39) |
missense |
probably benign |
|
|
IGL02167:Zfp777
|
APN |
6 |
48,021,460 (GRCm39) |
missense |
probably damaging |
0.98 |
|
IGL03150:Zfp777
|
APN |
6 |
48,021,059 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0238:Zfp777
|
UTSW |
6 |
48,001,903 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0238:Zfp777
|
UTSW |
6 |
48,001,903 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0372:Zfp777
|
UTSW |
6 |
48,021,410 (GRCm39) |
missense |
possibly damaging |
0.62 |
|
R0762:Zfp777
|
UTSW |
6 |
48,006,294 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1300:Zfp777
|
UTSW |
6 |
48,002,704 (GRCm39) |
missense |
probably benign |
0.43 |
|
R1727:Zfp777
|
UTSW |
6 |
48,020,824 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1906:Zfp777
|
UTSW |
6 |
48,018,995 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R2047:Zfp777
|
UTSW |
6 |
48,021,280 (GRCm39) |
missense |
probably benign |
|
|
R2097:Zfp777
|
UTSW |
6 |
48,021,176 (GRCm39) |
missense |
probably benign |
0.08 |
|
R2211:Zfp777
|
UTSW |
6 |
48,020,819 (GRCm39) |
missense |
possibly damaging |
0.79 |
|
R2898:Zfp777
|
UTSW |
6 |
48,002,594 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R3123:Zfp777
|
UTSW |
6 |
48,006,050 (GRCm39) |
unclassified |
probably benign |
|
|
R3832:Zfp777
|
UTSW |
6 |
48,021,149 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4019:Zfp777
|
UTSW |
6 |
48,019,046 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4077:Zfp777
|
UTSW |
6 |
48,002,456 (GRCm39) |
missense |
probably benign |
|
|
R4471:Zfp777
|
UTSW |
6 |
48,019,041 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5021:Zfp777
|
UTSW |
6 |
48,019,061 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5030:Zfp777
|
UTSW |
6 |
48,014,601 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5819:Zfp777
|
UTSW |
6 |
48,014,522 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6544:Zfp777
|
UTSW |
6 |
48,021,419 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R6736:Zfp777
|
UTSW |
6 |
48,001,790 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6971:Zfp777
|
UTSW |
6 |
48,001,625 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7240:Zfp777
|
UTSW |
6 |
48,021,383 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7258:Zfp777
|
UTSW |
6 |
48,002,731 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7586:Zfp777
|
UTSW |
6 |
48,006,152 (GRCm39) |
missense |
probably benign |
0.33 |
|
R7833:Zfp777
|
UTSW |
6 |
48,002,072 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7947:Zfp777
|
UTSW |
6 |
48,001,645 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8136:Zfp777
|
UTSW |
6 |
48,021,559 (GRCm39) |
missense |
probably benign |
0.25 |
|
R8348:Zfp777
|
UTSW |
6 |
48,006,101 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8448:Zfp777
|
UTSW |
6 |
48,006,101 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8942:Zfp777
|
UTSW |
6 |
48,006,125 (GRCm39) |
missense |
probably benign |
0.25 |
|
R8983:Zfp777
|
UTSW |
6 |
48,006,158 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9205:Zfp777
|
UTSW |
6 |
48,002,521 (GRCm39) |
missense |
probably benign |
0.07 |
|
R9397:Zfp777
|
UTSW |
6 |
48,021,190 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9562:Zfp777
|
UTSW |
6 |
48,021,580 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
R9565:Zfp777
|
UTSW |
6 |
48,021,580 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
RF008:Zfp777
|
UTSW |
6 |
48,018,982 (GRCm39) |
nonsense |
probably null |
|
|
Z1177:Zfp777
|
UTSW |
6 |
48,002,168 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- TCTGCCACTCACTACCTGTAGG -3'
(R):5'- AGATCGAGGTGCAGACCAAC -3'
Sequencing Primer
(F):5'- GCCCTGGAAGTGCCTTTGAATTC -3'
(R):5'- CTCAGAAGGTCTGGAGACACC -3'
|
| Posted On |
2020-06-30 |
Incidental Mutation