Mutagenetix > Incidental Mutation

Incidental Mutation 'R8942:Zfp777'

681039

Institutional Source

Beutler Lab

Gene Symbol

Zfp777

Ensembl Gene

ENSMUSG00000071477

Gene Name

zinc finger protein 777

Synonyms

2500002G23Rik

Accession Numbers

Essential gene?

Probably essential (E-score: 0.837) question?

Stock #

R8942 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

48001122-48025845 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 48006125 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 467 (E467G)

Ref Sequence

ENSEMBL: ENSMUSP00000110230 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095944] [ENSMUST00000114583]

AlphaFold

B9EKF4

Predicted Effect

probably benign
Transcript: ENSMUST00000095944
AA Change: E423G

PolyPhen 2 Score 0.248 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000093637
Gene: ENSMUSG00000071477
AA Change: E423G

Domain	Start	End	E-Value	Type
Pfam:DUF3669	177	256	4.3e-12	PFAM
KRAB	284	344	1.6e-29	SMART
low complexity region	422	433	N/A	INTRINSIC
coiled coil region	454	477	N/A	INTRINSIC
low complexity region	489	505	N/A	INTRINSIC
low complexity region	520	532	N/A	INTRINSIC
low complexity region	539	555	N/A	INTRINSIC
ZnF_C2H2	557	579	1.2e-5	SMART
ZnF_C2H2	585	607	3.8e-5	SMART
ZnF_C2H2	646	668	1.1e-6	SMART
ZnF_C2H2	674	696	4.6e-6	SMART
ZnF_C2H2	704	726	3.2e-7	SMART
ZnF_C2H2	732	754	3.3e-6	SMART
ZnF_C2H2	760	782	8.4e-6	SMART
ZnF_C2H2	788	810	4.9e-5	SMART
ZnF_C2H2	816	838	1.1e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000114583
AA Change: E467G

PolyPhen 2 Score 0.248 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000110230
Gene: ENSMUSG00000071477
AA Change: E467G

Domain	Start	End	E-Value	Type
low complexity region	2	24	N/A	INTRINSIC
Pfam:DUF3669	231	298	4.7e-12	PFAM
KRAB	328	388	3.96e-27	SMART
low complexity region	466	477	N/A	INTRINSIC
coiled coil region	498	521	N/A	INTRINSIC
low complexity region	533	549	N/A	INTRINSIC
low complexity region	564	576	N/A	INTRINSIC
low complexity region	583	599	N/A	INTRINSIC
ZnF_C2H2	601	623	2.95e-3	SMART
ZnF_C2H2	629	651	8.94e-3	SMART
ZnF_C2H2	690	712	2.43e-4	SMART
ZnF_C2H2	718	740	1.12e-3	SMART
ZnF_C2H2	748	770	7.49e-5	SMART
ZnF_C2H2	776	798	7.9e-4	SMART
ZnF_C2H2	804	826	1.95e-3	SMART
ZnF_C2H2	832	854	1.18e-2	SMART
ZnF_C2H2	860	882	2.53e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000148362

SMART Domains

Protein: ENSMUSP00000116303
Gene: ENSMUSG00000071477

Domain	Start	End	E-Value	Type
Pfam:DUF3669	40	107	6.1e-13	PFAM
KRAB	137	197	3.96e-27	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc4	A	T	14: 118,790,732 (GRCm39)	D833E	probably damaging	Het
Adgrl3	A	T	5: 81,796,568 (GRCm39)	I626L	probably benign	Het
AI182371	A	C	2: 34,990,622 (GRCm39)	L25R	probably damaging	Het
Atp2b2	G	T	6: 113,790,991 (GRCm39)	Q138K	probably benign	Het
Atp8b5	T	C	4: 43,353,658 (GRCm39)	F491L	probably damaging	Het
Bcap29	T	A	12: 31,684,353 (GRCm39)	M1L	probably damaging	Het
C1qtnf7	A	G	5: 43,773,583 (GRCm39)	D294G	probably benign	Het
Ccdc170	C	T	10: 4,484,044 (GRCm39)	A290V	probably benign	Het
Cfhr2	A	T	1: 139,741,292 (GRCm39)	L228I	probably benign	Het
Clip4	T	A	17: 72,170,768 (GRCm39)	V645D	probably benign	Het
Cyp4a31	C	T	4: 115,426,918 (GRCm39)	R173W	possibly damaging	Het
Dis3l	T	C	9: 64,214,875 (GRCm39)	K898R	probably benign	Het
Efr3b	G	T	12: 4,032,091 (GRCm39)	H172Q	possibly damaging	Het
Eipr1	A	T	12: 28,917,053 (GRCm39)	N374Y	probably damaging	Het
Exoc3l4	A	T	12: 111,392,002 (GRCm39)	E414D	possibly damaging	Het
Exoc3l4	G	T	12: 111,392,003 (GRCm39)	V415L	probably benign	Het
Faap100	G	T	11: 120,267,856 (GRCm39)	H306N	possibly damaging	Het
Gapvd1	T	C	2: 34,619,134 (GRCm39)	T80A	probably benign	Het
Gen1	A	G	12: 11,292,287 (GRCm39)	S501P	probably benign	Het
Gm10643	A	G	8: 84,790,799 (GRCm39)		probably benign	Het
Hecw1	A	T	13: 14,481,395 (GRCm39)	V445D	probably benign	Het
Hells	CT	C	19: 38,942,045 (GRCm39)		probably null	Het
Hnrnpr	T	G	4: 136,059,791 (GRCm39)	D197E	possibly damaging	Het
Hspb1	G	A	5: 135,916,928 (GRCm39)	V6M	probably damaging	Het
Iglon5	A	G	7: 43,126,315 (GRCm39)	V196A	probably benign	Het
Ints2	T	C	11: 86,103,720 (GRCm39)	T1142A	probably benign	Het
Katnip	T	G	7: 125,449,975 (GRCm39)	L1013R	probably damaging	Het
Kcnq2	T	A	2: 180,724,244 (GRCm39)	D587V	probably damaging	Het
Lmln	C	T	16: 32,901,330 (GRCm39)	P242L	probably damaging	Het
Mapt	G	A	11: 104,173,307 (GRCm39)		probably null	Het
Mrgpre	T	C	7: 143,335,002 (GRCm39)	Y167C		Het
Mtx2	A	T	2: 74,699,696 (GRCm39)	I156F	probably benign	Het
Nipbl	A	T	15: 8,381,104 (GRCm39)	S563T	probably benign	Het
Nmbr	C	T	10: 14,646,197 (GRCm39)	S357L	probably benign	Het
Or10ak14	A	T	4: 118,611,594 (GRCm39)	I49N	possibly damaging	Het
Or4e1	A	C	14: 52,700,692 (GRCm39)	I258S	probably damaging	Het
Or4f14	T	A	2: 111,743,207 (GRCm39)	I23F	probably benign	Het
Or4f60	T	C	2: 111,902,802 (GRCm39)	N42S	probably damaging	Het
Or4k15b	A	C	14: 50,272,602 (GRCm39)	L86R	probably damaging	Het
Or4p4	C	T	2: 88,483,308 (GRCm39)	L271F	possibly damaging	Het
Pctp	A	G	11: 89,875,554 (GRCm39)	M203T	possibly damaging	Het
Pip4k2c	A	G	10: 127,036,084 (GRCm39)	V261A	probably benign	Het
Pm20d1	G	T	1: 131,739,785 (GRCm39)	V378F	possibly damaging	Het
Ppcdc	C	T	9: 57,342,265 (GRCm39)	R19H	probably benign	Het
Pramel26	T	C	4: 143,536,861 (GRCm39)	N490S	probably benign	Het
Sdk1	A	G	5: 142,082,598 (GRCm39)	E1332G	probably damaging	Het
Sgce	G	T	6: 4,730,027 (GRCm39)	L66M	probably benign	Het
Sycp2l	G	T	13: 41,277,522 (GRCm39)		probably null	Het
Tars1	G	T	15: 11,384,183 (GRCm39)	H695N	probably benign	Het
Tdo2	A	G	3: 81,876,851 (GRCm39)	V107A	probably benign	Het
Tmem131l	A	T	3: 83,805,793 (GRCm39)	M1550K	possibly damaging	Het
Ttf2	A	C	3: 100,869,042 (GRCm39)	S431R	probably benign	Het
Unc80	A	G	1: 66,512,468 (GRCm39)	D126G	possibly damaging	Het
Usp17lb	C	T	7: 104,490,583 (GRCm39)	V115I	possibly damaging	Het
Vezf1	A	G	11: 87,972,553 (GRCm39)	T305A	probably benign	Het
Vmn1r45	A	T	6: 89,910,876 (GRCm39)	N31K	probably benign	Het
Wdr59	T	C	8: 112,211,808 (GRCm39)	K380E	probably benign	Het
Xylt1	T	A	7: 117,233,971 (GRCm39)	Y499*	probably null	Het
Zkscan4	C	A	13: 21,668,680 (GRCm39)	P406Q	probably benign	Het

Other mutations in Zfp777

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01530:Zfp777	APN	6	48,020,918 (GRCm39)	missense	probably damaging	1.00
IGL01916:Zfp777	APN	6	48,002,276 (GRCm39)	missense	probably damaging	1.00
IGL01959:Zfp777	APN	6	48,021,275 (GRCm39)	missense	probably benign
IGL02167:Zfp777	APN	6	48,021,460 (GRCm39)	missense	probably damaging	0.98
IGL03150:Zfp777	APN	6	48,021,059 (GRCm39)	missense	probably damaging	1.00
R0238:Zfp777	UTSW	6	48,001,903 (GRCm39)	missense	probably damaging	0.99
R0238:Zfp777	UTSW	6	48,001,903 (GRCm39)	missense	probably damaging	0.99
R0372:Zfp777	UTSW	6	48,021,410 (GRCm39)	missense	possibly damaging	0.62
R0762:Zfp777	UTSW	6	48,006,294 (GRCm39)	missense	probably damaging	1.00
R1300:Zfp777	UTSW	6	48,002,704 (GRCm39)	missense	probably benign	0.43
R1727:Zfp777	UTSW	6	48,020,824 (GRCm39)	missense	probably damaging	0.99
R1906:Zfp777	UTSW	6	48,018,995 (GRCm39)	missense	probably damaging	0.99
R2047:Zfp777	UTSW	6	48,021,280 (GRCm39)	missense	probably benign
R2097:Zfp777	UTSW	6	48,021,176 (GRCm39)	missense	probably benign	0.08
R2211:Zfp777	UTSW	6	48,020,819 (GRCm39)	missense	possibly damaging	0.79
R2898:Zfp777	UTSW	6	48,002,594 (GRCm39)	missense	probably damaging	0.97
R3123:Zfp777	UTSW	6	48,006,050 (GRCm39)	unclassified	probably benign
R3832:Zfp777	UTSW	6	48,021,149 (GRCm39)	missense	probably benign	0.00
R4019:Zfp777	UTSW	6	48,019,046 (GRCm39)	missense	probably damaging	1.00
R4077:Zfp777	UTSW	6	48,002,456 (GRCm39)	missense	probably benign
R4471:Zfp777	UTSW	6	48,019,041 (GRCm39)	missense	probably damaging	1.00
R5021:Zfp777	UTSW	6	48,019,061 (GRCm39)	missense	probably damaging	0.99
R5030:Zfp777	UTSW	6	48,014,601 (GRCm39)	missense	probably damaging	0.99
R5819:Zfp777	UTSW	6	48,014,522 (GRCm39)	missense	probably damaging	0.99
R6544:Zfp777	UTSW	6	48,021,419 (GRCm39)	missense	probably damaging	0.98
R6736:Zfp777	UTSW	6	48,001,790 (GRCm39)	missense	probably damaging	0.99
R6971:Zfp777	UTSW	6	48,001,625 (GRCm39)	missense	probably damaging	1.00
R7240:Zfp777	UTSW	6	48,021,383 (GRCm39)	missense	probably benign	0.00
R7258:Zfp777	UTSW	6	48,002,731 (GRCm39)	missense	probably damaging	0.99
R7586:Zfp777	UTSW	6	48,006,152 (GRCm39)	missense	probably benign	0.33
R7833:Zfp777	UTSW	6	48,002,072 (GRCm39)	missense	probably damaging	0.99
R7947:Zfp777	UTSW	6	48,001,645 (GRCm39)	missense	probably damaging	1.00
R8136:Zfp777	UTSW	6	48,021,559 (GRCm39)	missense	probably benign	0.25
R8151:Zfp777	UTSW	6	48,006,075 (GRCm39)	nonsense	probably null
R8348:Zfp777	UTSW	6	48,006,101 (GRCm39)	missense	probably damaging	0.99
R8448:Zfp777	UTSW	6	48,006,101 (GRCm39)	missense	probably damaging	0.99
R8983:Zfp777	UTSW	6	48,006,158 (GRCm39)	missense	probably damaging	1.00
R9205:Zfp777	UTSW	6	48,002,521 (GRCm39)	missense	probably benign	0.07
R9397:Zfp777	UTSW	6	48,021,190 (GRCm39)	missense	probably benign	0.00
R9562:Zfp777	UTSW	6	48,021,580 (GRCm39)	missense	possibly damaging	0.84
R9565:Zfp777	UTSW	6	48,021,580 (GRCm39)	missense	possibly damaging	0.84
RF008:Zfp777	UTSW	6	48,018,982 (GRCm39)	nonsense	probably null
Z1177:Zfp777	UTSW	6	48,002,168 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCCTGGAAGTGCCTTTGAATTC -3'
(R):5'- TGATGGCATCGTGATTAAGATCG -3'

Sequencing Primer
(F):5'- GGCAGAACAATTTCTTTGCTAGGC -3'
(R):5'- CATCGTGATTAAGATCGAGGTGC -3'

Posted On

2021-08-31