Incidental Mutation 'R9397:Zfp777'
| ID |
710880 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp777
|
| Ensembl Gene |
ENSMUSG00000071477 |
| Gene Name |
zinc finger protein 777 |
| Synonyms |
2500002G23Rik |
| MMRRC Submission |
|
| Accession Numbers |
|
| Essential gene? |
Probably essential
(E-score: 0.837)
|
| Stock # |
R9397 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
6 |
| Chromosomal Location |
48001122-48025845 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
G to T
at 48021190 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Lysine
at position 144
(T144K)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000093637
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000095944]
[ENSMUST00000114583]
[ENSMUST00000125385]
[ENSMUST00000147281]
|
| AlphaFold |
B9EKF4 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000095944
AA Change: T144K
PolyPhen 2
Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
|
| SMART Domains |
Protein: ENSMUSP00000093637
Gene: ENSMUSG00000071477
AA Change: T144K
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:DUF3669
|
177 |
256 |
4.3e-12 |
PFAM |
|
KRAB
|
284 |
344 |
1.6e-29 |
SMART |
|
low complexity region
|
422 |
433 |
N/A |
INTRINSIC |
|
coiled coil region
|
454 |
477 |
N/A |
INTRINSIC |
|
low complexity region
|
489 |
505 |
N/A |
INTRINSIC |
|
low complexity region
|
520 |
532 |
N/A |
INTRINSIC |
|
low complexity region
|
539 |
555 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
557 |
579 |
1.2e-5 |
SMART |
|
ZnF_C2H2
|
585 |
607 |
3.8e-5 |
SMART |
|
ZnF_C2H2
|
646 |
668 |
1.1e-6 |
SMART |
|
ZnF_C2H2
|
674 |
696 |
4.6e-6 |
SMART |
|
ZnF_C2H2
|
704 |
726 |
3.2e-7 |
SMART |
|
ZnF_C2H2
|
732 |
754 |
3.3e-6 |
SMART |
|
ZnF_C2H2
|
760 |
782 |
8.4e-6 |
SMART |
|
ZnF_C2H2
|
788 |
810 |
4.9e-5 |
SMART |
|
ZnF_C2H2
|
816 |
838 |
1.1e-4 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000114583
AA Change: T188K
PolyPhen 2
Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
|
| SMART Domains |
Protein: ENSMUSP00000110230
Gene: ENSMUSG00000071477
AA Change: T188K
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
2 |
24 |
N/A |
INTRINSIC |
|
Pfam:DUF3669
|
231 |
298 |
4.7e-12 |
PFAM |
|
KRAB
|
328 |
388 |
3.96e-27 |
SMART |
|
low complexity region
|
466 |
477 |
N/A |
INTRINSIC |
|
coiled coil region
|
498 |
521 |
N/A |
INTRINSIC |
|
low complexity region
|
533 |
549 |
N/A |
INTRINSIC |
|
low complexity region
|
564 |
576 |
N/A |
INTRINSIC |
|
low complexity region
|
583 |
599 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
601 |
623 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
629 |
651 |
8.94e-3 |
SMART |
|
ZnF_C2H2
|
690 |
712 |
2.43e-4 |
SMART |
|
ZnF_C2H2
|
718 |
740 |
1.12e-3 |
SMART |
|
ZnF_C2H2
|
748 |
770 |
7.49e-5 |
SMART |
|
ZnF_C2H2
|
776 |
798 |
7.9e-4 |
SMART |
|
ZnF_C2H2
|
804 |
826 |
1.95e-3 |
SMART |
|
ZnF_C2H2
|
832 |
854 |
1.18e-2 |
SMART |
|
ZnF_C2H2
|
860 |
882 |
2.53e-2 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000125385
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000147281
AA Change: T144K
PolyPhen 2
Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000148362
|
| SMART Domains |
Protein: ENSMUSP00000116303
Gene: ENSMUSG00000071477
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:DUF3669
|
40 |
107 |
6.1e-13 |
PFAM |
|
KRAB
|
137 |
197 |
3.96e-27 |
SMART |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 100.0%
- 10x: 99.8%
- 20x: 99.4%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 49 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930555G01Rik |
T |
G |
14: 5,051,639 (GRCm38) |
V66G |
possibly damaging |
Het |
| A630001G21Rik |
G |
T |
1: 85,653,002 (GRCm39) |
Y55* |
probably null |
Het |
| Abca8a |
G |
A |
11: 109,921,173 (GRCm39) |
T1474I |
probably damaging |
Het |
| Acaca |
T |
G |
11: 84,259,551 (GRCm39) |
I1962S |
probably damaging |
Het |
| Acap1 |
A |
T |
11: 69,775,672 (GRCm39) |
S455T |
probably damaging |
Het |
| Acbd7 |
G |
A |
2: 3,337,259 (GRCm39) |
|
probably null |
Het |
| Adamts9 |
A |
G |
6: 92,878,444 (GRCm39) |
L541P |
probably damaging |
Het |
| Ankrd49 |
T |
C |
9: 14,692,711 (GRCm39) |
N151S |
probably benign |
Het |
| Apcdd1 |
C |
T |
18: 63,055,731 (GRCm39) |
|
probably benign |
Het |
| Arhgap27 |
T |
C |
11: 103,231,115 (GRCm39) |
D58G |
probably damaging |
Het |
| Btbd18 |
G |
T |
2: 84,497,810 (GRCm39) |
A483S |
probably damaging |
Het |
| C2 |
A |
G |
17: 35,094,965 (GRCm39) |
S257P |
probably damaging |
Het |
| Catsper3 |
T |
A |
13: 55,946,725 (GRCm39) |
Y141N |
probably damaging |
Het |
| Cfap54 |
G |
A |
10: 92,833,147 (GRCm39) |
L1160F |
probably damaging |
Het |
| Chst13 |
G |
A |
6: 90,286,506 (GRCm39) |
P152L |
probably damaging |
Het |
| Cyp4f14 |
C |
T |
17: 33,130,516 (GRCm39) |
G141D |
probably damaging |
Het |
| Duox1 |
C |
T |
2: 122,150,783 (GRCm39) |
T286I |
possibly damaging |
Het |
| Epc2 |
G |
A |
2: 49,378,822 (GRCm39) |
G92E |
probably damaging |
Het |
| Fmnl3 |
C |
T |
15: 99,225,938 (GRCm39) |
|
probably null |
Het |
| Glyatl3 |
G |
A |
17: 41,223,565 (GRCm39) |
R59* |
probably null |
Het |
| Gm3633 |
T |
A |
14: 42,460,400 (GRCm39) |
Q181L |
|
Het |
| Guca1b |
C |
T |
17: 47,696,560 (GRCm39) |
T49M |
probably benign |
Het |
| Hdac9 |
T |
A |
12: 34,353,275 (GRCm39) |
H645L |
probably damaging |
Het |
| Hhip |
T |
C |
8: 80,719,112 (GRCm39) |
R472G |
probably benign |
Het |
| Ifi203 |
A |
G |
1: 173,765,547 (GRCm39) |
V9A |
probably damaging |
Het |
| Igkv9-123 |
A |
T |
6: 67,931,443 (GRCm39) |
V41D |
probably damaging |
Het |
| Il5ra |
T |
A |
6: 106,721,258 (GRCm39) |
T14S |
probably benign |
Het |
| Ints2 |
A |
G |
11: 86,135,311 (GRCm39) |
S368P |
probably benign |
Het |
| Kat6b |
T |
A |
14: 21,675,244 (GRCm39) |
S470T |
possibly damaging |
Het |
| Kmt2d |
A |
G |
15: 98,747,994 (GRCm39) |
V3110A |
unknown |
Het |
| Lama2 |
T |
C |
10: 26,981,117 (GRCm39) |
N1846D |
probably benign |
Het |
| Lrp1b |
T |
A |
2: 40,640,922 (GRCm39) |
E3507D |
|
Het |
| Ly6g6c |
A |
G |
17: 35,288,317 (GRCm39) |
N63S |
probably benign |
Het |
| Naprt |
C |
T |
15: 75,763,859 (GRCm39) |
V354I |
probably null |
Het |
| Nars1 |
A |
G |
18: 64,642,327 (GRCm39) |
S172P |
probably benign |
Het |
| Odr4 |
C |
T |
1: 150,238,616 (GRCm39) |
A434T |
probably damaging |
Het |
| Or6c5b |
A |
G |
10: 129,245,909 (GRCm39) |
R225G |
possibly damaging |
Het |
| Or7e177 |
T |
C |
9: 20,211,748 (GRCm39) |
I85T |
possibly damaging |
Het |
| Pcdhb6 |
A |
G |
18: 37,469,353 (GRCm39) |
D758G |
probably benign |
Het |
| Pkp4 |
G |
A |
2: 59,148,856 (GRCm39) |
W536* |
probably null |
Het |
| Polr3b |
C |
T |
10: 84,467,653 (GRCm39) |
L78F |
possibly damaging |
Het |
| Rasa4 |
T |
A |
5: 136,129,836 (GRCm39) |
V272E |
possibly damaging |
Het |
| Syne2 |
A |
G |
12: 76,040,849 (GRCm39) |
T3836A |
possibly damaging |
Het |
| Tbpl2 |
C |
T |
2: 23,966,070 (GRCm39) |
V308I |
possibly damaging |
Het |
| Tcea1 |
T |
A |
1: 4,962,141 (GRCm39) |
I176K |
probably damaging |
Het |
| Try5 |
A |
T |
6: 41,289,314 (GRCm39) |
F87L |
probably benign |
Het |
| Ttc8 |
G |
T |
12: 98,942,692 (GRCm39) |
G411* |
probably null |
Het |
| Vmn2r115 |
G |
A |
17: 23,564,152 (GRCm39) |
W108* |
probably null |
Het |
| Zdhhc13 |
C |
T |
7: 48,476,628 (GRCm39) |
T581I |
probably benign |
Het |
|
| Other mutations in Zfp777 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01530:Zfp777
|
APN |
6 |
48,020,918 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01916:Zfp777
|
APN |
6 |
48,002,276 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01959:Zfp777
|
APN |
6 |
48,021,275 (GRCm39) |
missense |
probably benign |
|
|
IGL02167:Zfp777
|
APN |
6 |
48,021,460 (GRCm39) |
missense |
probably damaging |
0.98 |
|
IGL03150:Zfp777
|
APN |
6 |
48,021,059 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0238:Zfp777
|
UTSW |
6 |
48,001,903 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0238:Zfp777
|
UTSW |
6 |
48,001,903 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0372:Zfp777
|
UTSW |
6 |
48,021,410 (GRCm39) |
missense |
possibly damaging |
0.62 |
|
R0762:Zfp777
|
UTSW |
6 |
48,006,294 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1300:Zfp777
|
UTSW |
6 |
48,002,704 (GRCm39) |
missense |
probably benign |
0.43 |
|
R1727:Zfp777
|
UTSW |
6 |
48,020,824 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1906:Zfp777
|
UTSW |
6 |
48,018,995 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R2047:Zfp777
|
UTSW |
6 |
48,021,280 (GRCm39) |
missense |
probably benign |
|
|
R2097:Zfp777
|
UTSW |
6 |
48,021,176 (GRCm39) |
missense |
probably benign |
0.08 |
|
R2211:Zfp777
|
UTSW |
6 |
48,020,819 (GRCm39) |
missense |
possibly damaging |
0.79 |
|
R2898:Zfp777
|
UTSW |
6 |
48,002,594 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R3123:Zfp777
|
UTSW |
6 |
48,006,050 (GRCm39) |
unclassified |
probably benign |
|
|
R3832:Zfp777
|
UTSW |
6 |
48,021,149 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4019:Zfp777
|
UTSW |
6 |
48,019,046 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4077:Zfp777
|
UTSW |
6 |
48,002,456 (GRCm39) |
missense |
probably benign |
|
|
R4471:Zfp777
|
UTSW |
6 |
48,019,041 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5021:Zfp777
|
UTSW |
6 |
48,019,061 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5030:Zfp777
|
UTSW |
6 |
48,014,601 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5819:Zfp777
|
UTSW |
6 |
48,014,522 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6544:Zfp777
|
UTSW |
6 |
48,021,419 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R6736:Zfp777
|
UTSW |
6 |
48,001,790 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6971:Zfp777
|
UTSW |
6 |
48,001,625 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7240:Zfp777
|
UTSW |
6 |
48,021,383 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7258:Zfp777
|
UTSW |
6 |
48,002,731 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7586:Zfp777
|
UTSW |
6 |
48,006,152 (GRCm39) |
missense |
probably benign |
0.33 |
|
R7833:Zfp777
|
UTSW |
6 |
48,002,072 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7947:Zfp777
|
UTSW |
6 |
48,001,645 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8136:Zfp777
|
UTSW |
6 |
48,021,559 (GRCm39) |
missense |
probably benign |
0.25 |
|
R8151:Zfp777
|
UTSW |
6 |
48,006,075 (GRCm39) |
nonsense |
probably null |
|
|
R8348:Zfp777
|
UTSW |
6 |
48,006,101 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8448:Zfp777
|
UTSW |
6 |
48,006,101 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8942:Zfp777
|
UTSW |
6 |
48,006,125 (GRCm39) |
missense |
probably benign |
0.25 |
|
R8983:Zfp777
|
UTSW |
6 |
48,006,158 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9205:Zfp777
|
UTSW |
6 |
48,002,521 (GRCm39) |
missense |
probably benign |
0.07 |
|
R9562:Zfp777
|
UTSW |
6 |
48,021,580 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
R9565:Zfp777
|
UTSW |
6 |
48,021,580 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
RF008:Zfp777
|
UTSW |
6 |
48,018,982 (GRCm39) |
nonsense |
probably null |
|
|
Z1177:Zfp777
|
UTSW |
6 |
48,002,168 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- CCAGAGTCAGTAGTCGCATG -3'
(R):5'- TCAGAAAGGACCTTCACTCCTG -3'
Sequencing Primer
(F):5'- AGTCAGTAGTCGCATGGCCTG -3'
(R):5'- GTATCCTGCCACTTCTGAGCAAG -3'
|
| Posted On |
2022-04-18 |
Incidental Mutation