Mutagenetix > Incidental Mutation

Incidental Mutation 'R8249:Mroh7'

640318

Institutional Source

Beutler Lab

Gene Symbol

Mroh7

Ensembl Gene

ENSMUSG00000047502

Gene Name

maestro heat-like repeat family member 7

Synonyms

Heatr8, Gm1027, LOC381538

MMRRC Submission

067649-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8249 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

106537614-106588122 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 106578409 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 90 (T90A)

Ref Sequence

ENSEMBL: ENSMUSP00000102382 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000106770] [ENSMUST00000145044] [ENSMUST00000148281]

AlphaFold

A2AVR2

Predicted Effect

probably benign
Transcript: ENSMUST00000106770
AA Change: T90A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000102382
Gene: ENSMUSG00000047502
AA Change: T90A

Domain	Start	End	E-Value	Type
low complexity region	39	61	N/A	INTRINSIC
low complexity region	318	332	N/A	INTRINSIC
low complexity region	563	573	N/A	INTRINSIC
SCOP:d1b3ua_	634	1218	6e-9	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000145044

Predicted Effect

probably benign
Transcript: ENSMUST00000148281

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	A	G	1: 71,360,971 (GRCm39)	S441P	probably benign	Het
Adck2	C	T	6: 39,562,667 (GRCm39)	R513*	probably null	Het
Agbl2	G	A	2: 90,627,908 (GRCm39)	G238R	probably damaging	Het
Arhgap12	A	T	18: 6,027,635 (GRCm39)	I736N	probably damaging	Het
AW554918	C	T	18: 25,472,775 (GRCm39)	T193I	probably benign	Het
Bscl2	T	C	19: 8,823,884 (GRCm39)	Y270H	probably damaging	Het
Btd	T	C	14: 31,387,905 (GRCm39)	Y112H	probably damaging	Het
Camk2d	C	T	3: 126,591,378 (GRCm39)	H283Y	probably damaging	Het
Cnga1	T	C	5: 72,762,737 (GRCm39)	Y259C	probably benign	Het
Dnm3	A	T	1: 162,305,312 (GRCm39)	C27*	probably null	Het
Fbxo48	T	A	11: 16,903,433 (GRCm39)	S20T	possibly damaging	Het
Fkbp6	A	G	5: 135,378,806 (GRCm39)	S14P	possibly damaging	Het
Gcfc2	T	C	6: 81,933,932 (GRCm39)	F730L	probably benign	Het
Gm136	C	A	4: 34,750,955 (GRCm39)	R106L	probably benign	Het
Gpr165	C	A	X: 95,757,623 (GRCm39)	D7E	probably benign	Het
Grin2c	C	T	11: 115,144,663 (GRCm39)	R621Q	probably damaging	Het
H2-M11	T	A	17: 36,859,900 (GRCm39)	W298R	probably damaging	Het
Hars2	A	T	18: 36,921,054 (GRCm39)	I209F	probably damaging	Het
Hmcn1	A	T	1: 150,695,117 (GRCm39)	N262K	probably benign	Het
Hycc2	G	A	1: 58,573,796 (GRCm39)	S336L	probably benign	Het
Ifit1	T	A	19: 34,618,389 (GRCm39)		probably null	Het
Ighv1-85	T	C	12: 115,963,844 (GRCm39)	D52G	probably benign	Het
Kit	T	C	5: 75,802,068 (GRCm39)	V657A	probably damaging	Het
Krt32	T	C	11: 99,977,548 (GRCm39)	I173V	probably benign	Het
Lrfn3	GC	GCCAGGGGAGGTGAGCGGTGGGTCACCTCCC	7: 30,059,298 (GRCm39)		probably null	Het
Lrp1	C	A	10: 127,441,412 (GRCm39)	S237I	probably benign	Het
Map3k9	T	C	12: 81,827,551 (GRCm39)	D33G	unknown	Het
Mterf1a	A	C	5: 3,941,550 (GRCm39)	I106S	probably damaging	Het
Mtrf1	C	A	14: 79,638,919 (GRCm39)	A17E	probably benign	Het
Musk	T	G	4: 58,368,926 (GRCm39)	L635R	probably damaging	Het
Naxe	A	T	3: 87,965,695 (GRCm39)	W35R	possibly damaging	Het
Nlrp9c	T	A	7: 26,074,778 (GRCm39)	K806*	probably null	Het
Pi4k2a	A	G	19: 42,103,501 (GRCm39)	E337G	probably benign	Het
Pwwp2b	G	T	7: 138,834,759 (GRCm39)	G67C	probably damaging	Het
Rftn1	G	T	17: 50,354,408 (GRCm39)	A318D	probably damaging	Het
Scn10a	T	A	9: 119,446,840 (GRCm39)	Y1396F	probably damaging	Het
Sdk1	A	T	5: 142,173,770 (GRCm39)		probably null	Het
Serpina1f	C	T	12: 103,660,027 (GRCm39)	G85E	probably damaging	Het
Tceanc2	G	A	4: 107,036,190 (GRCm39)		probably benign	Het
Tmem156	T	A	5: 65,232,969 (GRCm39)	R186*	probably null	Het
Trbv16	A	G	6: 41,128,932 (GRCm39)	T39A	possibly damaging	Het
Tti1	C	G	2: 157,842,635 (GRCm39)	S798T	probably benign	Het
Unc80	A	T	1: 66,658,650 (GRCm39)	M1656L	probably benign	Het
Wnt2	C	A	6: 18,030,284 (GRCm39)	M1I	probably null	Het

Other mutations in Mroh7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01722:Mroh7	APN	4	106,560,358 (GRCm39)	missense	probably benign	0.00
IGL01729:Mroh7	APN	4	106,561,402 (GRCm39)	missense	possibly damaging	0.66
IGL01834:Mroh7	APN	4	106,538,071 (GRCm39)	missense	probably benign	0.00
IGL02003:Mroh7	APN	4	106,559,726 (GRCm39)	missense	probably damaging	0.96
IGL02135:Mroh7	APN	4	106,559,707 (GRCm39)	missense	probably damaging	1.00
IGL02335:Mroh7	APN	4	106,564,979 (GRCm39)	missense	probably damaging	1.00
IGL02532:Mroh7	APN	4	106,577,788 (GRCm39)	missense	probably benign	0.04
IGL02896:Mroh7	APN	4	106,557,013 (GRCm39)	missense	possibly damaging	0.94
IGL03066:Mroh7	APN	4	106,549,595 (GRCm39)	missense	possibly damaging	0.85
IGL03298:Mroh7	APN	4	106,571,288 (GRCm39)	nonsense	probably null
holy	UTSW	4	106,567,152 (GRCm39)	splice site	probably null
moley	UTSW	4	106,551,509 (GRCm39)	splice site	probably null
P0016:Mroh7	UTSW	4	106,565,054 (GRCm39)	critical splice acceptor site	probably null
R0019:Mroh7	UTSW	4	106,578,623 (GRCm39)	missense	probably benign	0.07
R0094:Mroh7	UTSW	4	106,560,381 (GRCm39)	missense	probably damaging	0.98
R0105:Mroh7	UTSW	4	106,568,467 (GRCm39)	missense	possibly damaging	0.49
R0105:Mroh7	UTSW	4	106,568,467 (GRCm39)	missense	possibly damaging	0.49
R0515:Mroh7	UTSW	4	106,548,861 (GRCm39)	missense	probably benign	0.01
R0828:Mroh7	UTSW	4	106,557,073 (GRCm39)	missense	probably damaging	0.99
R0831:Mroh7	UTSW	4	106,537,990 (GRCm39)	missense	possibly damaging	0.92
R1107:Mroh7	UTSW	4	106,564,791 (GRCm39)	splice site	probably null
R1301:Mroh7	UTSW	4	106,577,692 (GRCm39)	missense	probably damaging	0.99
R1456:Mroh7	UTSW	4	106,552,338 (GRCm39)	splice site	probably benign
R1491:Mroh7	UTSW	4	106,560,255 (GRCm39)	missense	probably benign	0.11
R1540:Mroh7	UTSW	4	106,560,273 (GRCm39)	missense	probably benign	0.11
R1560:Mroh7	UTSW	4	106,568,451 (GRCm39)	missense	possibly damaging	0.78
R1645:Mroh7	UTSW	4	106,577,865 (GRCm39)	missense	probably benign	0.19
R1804:Mroh7	UTSW	4	106,551,589 (GRCm39)	missense	possibly damaging	0.76
R2162:Mroh7	UTSW	4	106,557,378 (GRCm39)	missense	probably damaging	0.96
R2265:Mroh7	UTSW	4	106,578,124 (GRCm39)	missense	probably benign	0.01
R2866:Mroh7	UTSW	4	106,548,287 (GRCm39)	missense	probably damaging	1.00
R3716:Mroh7	UTSW	4	106,561,407 (GRCm39)	missense	probably benign	0.25
R3718:Mroh7	UTSW	4	106,561,407 (GRCm39)	missense	probably benign	0.25
R4530:Mroh7	UTSW	4	106,577,634 (GRCm39)	missense	possibly damaging	0.71
R4661:Mroh7	UTSW	4	106,548,710 (GRCm39)	critical splice donor site	probably null
R4706:Mroh7	UTSW	4	106,548,821 (GRCm39)	missense	possibly damaging	0.86
R4910:Mroh7	UTSW	4	106,567,152 (GRCm39)	splice site	probably null
R4965:Mroh7	UTSW	4	106,548,184 (GRCm39)	missense	possibly damaging	0.77
R4969:Mroh7	UTSW	4	106,538,070 (GRCm39)	missense	probably benign
R4971:Mroh7	UTSW	4	106,548,749 (GRCm39)	missense	probably benign	0.04
R5083:Mroh7	UTSW	4	106,547,515 (GRCm39)	missense	probably benign	0.03
R5207:Mroh7	UTSW	4	106,578,583 (GRCm39)	missense	probably damaging	0.97
R5364:Mroh7	UTSW	4	106,548,840 (GRCm39)	missense	probably benign	0.10
R5392:Mroh7	UTSW	4	106,568,448 (GRCm39)	critical splice donor site	probably null
R5630:Mroh7	UTSW	4	106,577,764 (GRCm39)	missense	possibly damaging	0.71
R5691:Mroh7	UTSW	4	106,559,815 (GRCm39)	missense	probably damaging	0.96
R5703:Mroh7	UTSW	4	106,565,757 (GRCm39)	missense	possibly damaging	0.77
R5707:Mroh7	UTSW	4	106,539,082 (GRCm39)	missense	possibly damaging	0.73
R5919:Mroh7	UTSW	4	106,551,509 (GRCm39)	splice site	probably null
R5979:Mroh7	UTSW	4	106,578,123 (GRCm39)	missense	probably benign	0.00
R6479:Mroh7	UTSW	4	106,560,385 (GRCm39)	missense	possibly damaging	0.75
R6520:Mroh7	UTSW	4	106,578,460 (GRCm39)	missense	probably benign	0.00
R6657:Mroh7	UTSW	4	106,559,697 (GRCm39)	nonsense	probably null
R6732:Mroh7	UTSW	4	106,537,910 (GRCm39)	frame shift	probably null
R6817:Mroh7	UTSW	4	106,571,312 (GRCm39)	missense	probably benign	0.00
R6980:Mroh7	UTSW	4	106,557,434 (GRCm39)	missense	probably benign	0.05
R7062:Mroh7	UTSW	4	106,541,177 (GRCm39)	missense	probably damaging	1.00
R7116:Mroh7	UTSW	4	106,568,517 (GRCm39)	missense	probably benign	0.07
R7134:Mroh7	UTSW	4	106,577,791 (GRCm39)	missense	probably damaging	0.99
R7169:Mroh7	UTSW	4	106,548,836 (GRCm39)	missense	probably damaging	0.99
R7419:Mroh7	UTSW	4	106,541,115 (GRCm39)	missense	probably benign
R7516:Mroh7	UTSW	4	106,548,316 (GRCm39)	missense	probably benign	0.00
R7525:Mroh7	UTSW	4	106,566,899 (GRCm39)	missense	probably benign	0.22
R7540:Mroh7	UTSW	4	106,577,595 (GRCm39)	missense	possibly damaging	0.85
R7849:Mroh7	UTSW	4	106,578,287 (GRCm39)	missense	probably benign
R7920:Mroh7	UTSW	4	106,564,773 (GRCm39)	missense	probably benign
R7998:Mroh7	UTSW	4	106,568,478 (GRCm39)	missense	probably benign	0.02
R8026:Mroh7	UTSW	4	106,578,634 (GRCm39)	missense	probably benign	0.01
R8122:Mroh7	UTSW	4	106,559,726 (GRCm39)	missense	probably damaging	0.96
R9188:Mroh7	UTSW	4	106,566,789 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CTTGCTTGAGAAGGACTTGGAG -3'
(R):5'- ACCAAGTCCTAACTCCTGTGAG -3'

Sequencing Primer
(F):5'- CTTGGAGACATCCTCAGAGCATG -3'
(R):5'- GTGAGGTTCCAGGAATTATGTCCAAC -3'

Posted On

2020-07-28