Mutagenetix > Incidental Mutation

Incidental Mutation 'R9311:Lama5'

705537

Institutional Source

Beutler Lab

Gene Symbol

Lama5

Ensembl Gene

ENSMUSG00000015647

Gene Name

laminin, alpha 5

Synonyms

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9311 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

179818166-179867652 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to G at 179838275 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000015791 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000015791] [ENSMUST00000015791] [ENSMUST00000015791]

AlphaFold

no structure available at present

PDB Structure

LAMININ ALPHA5 CHAIN N-TERMINAL FRAGMENT [X-RAY DIFFRACTION]

Predicted Effect

probably null
Transcript: ENSMUST00000015791

SMART Domains

Protein: ENSMUSP00000015791
Gene: ENSMUSG00000015647

Domain	Start	End	E-Value	Type
signal peptide	1	40	N/A	INTRINSIC
LamNT	44	303	1.06e-132	SMART
EGF_Lam	305	361	4.35e-6	SMART
EGF_Lam	364	431	5.78e-11	SMART
EGF_Lam	434	476	1.32e-5	SMART
EGF_Lam	500	544	8.63e-10	SMART
EGF_Lam	547	590	1.16e-10	SMART
EGF_Lam	593	635	4.63e-10	SMART
EGF_Lam	638	680	6.25e-7	SMART
EGF_Lam	683	726	3.1e-11	SMART
EGF_Lam	730	779	2.99e-4	SMART
EGF_Lam	782	831	4.66e-6	SMART
EGF_Lam	834	878	3.48e-5	SMART
low complexity region	1261	1273	N/A	INTRINSIC
EGF_Lam	1443	1486	7.01e-10	SMART
EGF_like	1489	1530	3.64e-1	SMART
EGF_Lam	1533	1579	8.56e-14	SMART
EGF_Lam	1582	1630	1.86e-14	SMART
LamB	1689	1819	5.86e-61	SMART
EGF_like	1818	1862	2.74e0	SMART
EGF_Lam	1865	1912	3.32e-11	SMART
EGF_Lam	1915	1968	1.61e-9	SMART
EGF_Lam	1971	2022	6.39e-13	SMART
EGF_Lam	2025	2069	1.94e-12	SMART
EGF_Lam	2072	2116	1.35e-11	SMART
EGF_like	2103	2145	3.1e1	SMART
EGF_Lam	2119	2166	1.18e-2	SMART
Pfam:Laminin_I	2189	2453	1.7e-65	PFAM
low complexity region	2532	2548	N/A	INTRINSIC
low complexity region	2557	2569	N/A	INTRINSIC
low complexity region	2632	2641	N/A	INTRINSIC
low complexity region	2663	2676	N/A	INTRINSIC
LamG	2760	2912	3.97e-8	SMART
LamG	2966	3103	1.78e-10	SMART
LamG	3149	3274	1.11e-20	SMART
LamG	3359	3497	4.05e-23	SMART
LamG	3539	3670	3e-26	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000015791

SMART Domains

Protein: ENSMUSP00000015791
Gene: ENSMUSG00000015647

Domain	Start	End	E-Value	Type
signal peptide	1	40	N/A	INTRINSIC
LamNT	44	303	1.06e-132	SMART
EGF_Lam	305	361	4.35e-6	SMART
EGF_Lam	364	431	5.78e-11	SMART
EGF_Lam	434	476	1.32e-5	SMART
EGF_Lam	500	544	8.63e-10	SMART
EGF_Lam	547	590	1.16e-10	SMART
EGF_Lam	593	635	4.63e-10	SMART
EGF_Lam	638	680	6.25e-7	SMART
EGF_Lam	683	726	3.1e-11	SMART
EGF_Lam	730	779	2.99e-4	SMART
EGF_Lam	782	831	4.66e-6	SMART
EGF_Lam	834	878	3.48e-5	SMART
low complexity region	1261	1273	N/A	INTRINSIC
EGF_Lam	1443	1486	7.01e-10	SMART
EGF_like	1489	1530	3.64e-1	SMART
EGF_Lam	1533	1579	8.56e-14	SMART
EGF_Lam	1582	1630	1.86e-14	SMART
LamB	1689	1819	5.86e-61	SMART
EGF_like	1818	1862	2.74e0	SMART
EGF_Lam	1865	1912	3.32e-11	SMART
EGF_Lam	1915	1968	1.61e-9	SMART
EGF_Lam	1971	2022	6.39e-13	SMART
EGF_Lam	2025	2069	1.94e-12	SMART
EGF_Lam	2072	2116	1.35e-11	SMART
EGF_like	2103	2145	3.1e1	SMART
EGF_Lam	2119	2166	1.18e-2	SMART
Pfam:Laminin_I	2189	2453	1.7e-65	PFAM
low complexity region	2532	2548	N/A	INTRINSIC
low complexity region	2557	2569	N/A	INTRINSIC
low complexity region	2632	2641	N/A	INTRINSIC
low complexity region	2663	2676	N/A	INTRINSIC
LamG	2760	2912	3.97e-8	SMART
LamG	2966	3103	1.78e-10	SMART
LamG	3149	3274	1.11e-20	SMART
LamG	3359	3497	4.05e-23	SMART
LamG	3539	3670	3e-26	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000015791

SMART Domains

Protein: ENSMUSP00000015791
Gene: ENSMUSG00000015647

Domain	Start	End	E-Value	Type
signal peptide	1	40	N/A	INTRINSIC
LamNT	44	303	1.06e-132	SMART
EGF_Lam	305	361	4.35e-6	SMART
EGF_Lam	364	431	5.78e-11	SMART
EGF_Lam	434	476	1.32e-5	SMART
EGF_Lam	500	544	8.63e-10	SMART
EGF_Lam	547	590	1.16e-10	SMART
EGF_Lam	593	635	4.63e-10	SMART
EGF_Lam	638	680	6.25e-7	SMART
EGF_Lam	683	726	3.1e-11	SMART
EGF_Lam	730	779	2.99e-4	SMART
EGF_Lam	782	831	4.66e-6	SMART
EGF_Lam	834	878	3.48e-5	SMART
low complexity region	1261	1273	N/A	INTRINSIC
EGF_Lam	1443	1486	7.01e-10	SMART
EGF_like	1489	1530	3.64e-1	SMART
EGF_Lam	1533	1579	8.56e-14	SMART
EGF_Lam	1582	1630	1.86e-14	SMART
LamB	1689	1819	5.86e-61	SMART
EGF_like	1818	1862	2.74e0	SMART
EGF_Lam	1865	1912	3.32e-11	SMART
EGF_Lam	1915	1968	1.61e-9	SMART
EGF_Lam	1971	2022	6.39e-13	SMART
EGF_Lam	2025	2069	1.94e-12	SMART
EGF_Lam	2072	2116	1.35e-11	SMART
EGF_like	2103	2145	3.1e1	SMART
EGF_Lam	2119	2166	1.18e-2	SMART
Pfam:Laminin_I	2189	2453	1.7e-65	PFAM
low complexity region	2532	2548	N/A	INTRINSIC
low complexity region	2557	2569	N/A	INTRINSIC
low complexity region	2632	2641	N/A	INTRINSIC
low complexity region	2663	2676	N/A	INTRINSIC
LamG	2760	2912	3.97e-8	SMART
LamG	2966	3103	1.78e-10	SMART
LamG	3149	3274	1.11e-20	SMART
LamG	3359	3497	4.05e-23	SMART
LamG	3539	3670	3e-26	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (61/61)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit disrupted basal laminae leading to exencephaly, syndactyly, placentopathy, kidney defects, abnormal lobar septation with absence of a visceral pleural membrane, and lethality in late gestation. [provided by MGI curators]

Allele List at MGI

All alleles(49) : Targeted(5) Gene trapped(44)

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A4gnt	T	C	9: 99,495,816 (GRCm39)	I84T	possibly damaging	Het
Adad2	G	T	8: 120,341,986 (GRCm39)	R268L	probably damaging	Het
Agxt2	A	T	15: 10,380,733 (GRCm39)	N208I	probably damaging	Het
Brsk1	G	T	7: 4,709,722 (GRCm39)		probably null	Het
Cd40	A	T	2: 164,912,667 (GRCm39)	Q235L	possibly damaging	Het
Cdcp3	A	T	7: 130,859,490 (GRCm39)	D1137V	unknown	Het
Cln6	T	C	9: 62,757,900 (GRCm39)	Y220H	probably damaging	Het
Cngb1	A	T	8: 96,010,794 (GRCm39)		probably null	Het
Cpne1	A	T	2: 155,919,723 (GRCm39)	V277E	probably damaging	Het
Csf2rb2	C	T	15: 78,176,735 (GRCm39)		probably null	Het
Cyp4f15	A	G	17: 32,905,139 (GRCm39)	T41A	probably benign	Het
Dab1	T	C	4: 104,369,463 (GRCm39)		probably null	Het
Eif4e1b	C	A	13: 54,932,332 (GRCm39)	H56N	probably benign	Het
Elp3	A	T	14: 65,823,788 (GRCm39)	D78E	probably benign	Het
Ephx3	C	G	17: 32,408,290 (GRCm39)	D45H	probably benign	Het
Gabarap	T	A	11: 69,882,549 (GRCm39)	V4E	probably benign	Het
Gm5105	T	A	3: 137,755,418 (GRCm39)	D56V	unknown	Het
Gosr2	G	T	11: 103,574,693 (GRCm39)	H134Q	probably damaging	Het
Ifi204	A	G	1: 173,589,215 (GRCm39)	V72A	possibly damaging	Het
Irx1	A	G	13: 72,107,416 (GRCm39)	V422A	probably benign	Het
Kcne4	C	A	1: 78,795,824 (GRCm39)	D157E	probably benign	Het
Kctd13	A	G	7: 126,541,345 (GRCm39)	N195S	probably damaging	Het
Kirrel1	A	G	3: 87,005,123 (GRCm39)	V75A	probably benign	Het
Klkb1	T	C	8: 45,722,983 (GRCm39)	T625A	probably benign	Het
Lifr	A	G	15: 7,208,418 (GRCm39)	I599V	possibly damaging	Het
Lin52	A	T	12: 84,576,470 (GRCm39)	E101V	probably damaging	Het
Liph	T	C	16: 21,802,680 (GRCm39)	I130V	probably benign	Het
Liph	C	T	16: 21,774,913 (GRCm39)	R428Q	probably damaging	Het
Lrrc4c	A	C	2: 97,461,080 (GRCm39)	I569L	possibly damaging	Het
Mnat1	G	A	12: 73,214,916 (GRCm39)	V78I	probably benign	Het
Msln	C	T	17: 25,971,990 (GRCm39)	D76N	probably benign	Het
Myh7b	A	G	2: 155,463,253 (GRCm39)	H495R	probably damaging	Het
Ndst4	C	T	3: 125,518,385 (GRCm39)	S354L	probably benign	Het
Nfu1	T	C	6: 86,986,926 (GRCm39)	V15A	probably benign	Het
Nphp4	T	C	4: 152,608,714 (GRCm39)	S441P	probably damaging	Het
Nuak1	T	C	10: 84,214,090 (GRCm39)		probably null	Het
Or1j20	A	T	2: 36,760,405 (GRCm39)	I276F	probably damaging	Het
Or5h27	A	T	16: 59,006,106 (GRCm39)	C247S	unknown	Het
Or5w22	A	G	2: 87,362,358 (GRCm39)		probably benign	Het
Or8b3b	A	G	9: 38,583,925 (GRCm39)	S272P	probably damaging	Het
Palld	A	G	8: 61,978,189 (GRCm39)	V1109A	unknown	Het
Pik3c3	G	A	18: 30,445,666 (GRCm39)	R551H	probably benign	Het
Plcb1	G	A	2: 135,189,385 (GRCm39)	V838I	probably benign	Het
Plppr4	G	T	3: 117,119,518 (GRCm39)	T297K	probably damaging	Het
Ptprd	A	G	4: 76,051,320 (GRCm39)	I67T	probably benign	Het
Rb1cc1	T	A	1: 6,310,539 (GRCm39)	N312K	probably damaging	Het
Sh3glb1	A	C	3: 144,397,659 (GRCm39)		probably null	Het
Siglec1	C	T	2: 130,916,013 (GRCm39)	C1283Y	probably damaging	Het
Spns1	T	C	7: 125,972,995 (GRCm39)	I204V	probably damaging	Het
Sprr5	T	G	3: 92,440,397 (GRCm39)	Q14P	unknown	Het
Supt6	T	C	11: 78,116,284 (GRCm39)	Y693C	probably damaging	Het
Taar9	A	G	10: 23,985,152 (GRCm39)	V94A	probably damaging	Het
Tchp	T	C	5: 114,846,877 (GRCm39)	S55P	probably benign	Het
Tmem132b	A	G	5: 125,863,029 (GRCm39)	H678R	possibly damaging	Het
Tnr	G	C	1: 159,677,663 (GRCm39)	G16A	probably benign	Het
Top3b	T	C	16: 16,700,563 (GRCm39)		probably null	Het
Tpmt	A	G	13: 47,185,892 (GRCm39)		probably null	Het
Treh	G	A	9: 44,592,655 (GRCm39)	V87I	probably benign	Het
Ttc4	T	C	4: 106,535,963 (GRCm39)	D33G	probably benign	Het
Usp47	A	G	7: 111,703,257 (GRCm39)	D1171G	probably benign	Het
Vmn2r61	G	T	7: 41,950,092 (GRCm39)	L837F	possibly damaging	Het
Vmn2r86	T	A	10: 130,288,440 (GRCm39)	N354Y	probably damaging	Het
Vmn2r88	C	G	14: 51,650,503 (GRCm39)	A72G	probably benign	Het
Zscan4b	C	A	7: 10,635,950 (GRCm39)	V126F	probably damaging	Het

Other mutations in Lama5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01067:Lama5	APN	2	179,818,336 (GRCm39)	unclassified	probably benign
IGL01370:Lama5	APN	2	179,839,193 (GRCm39)	missense	possibly damaging	0.87
IGL01474:Lama5	APN	2	179,838,363 (GRCm39)	missense	probably damaging	1.00
IGL01614:Lama5	APN	2	179,822,657 (GRCm39)	missense	probably damaging	1.00
IGL01941:Lama5	APN	2	179,834,185 (GRCm39)	missense	possibly damaging	0.71
IGL01953:Lama5	APN	2	179,832,497 (GRCm39)	missense	probably damaging	0.97
IGL02093:Lama5	APN	2	179,830,380 (GRCm39)	missense	probably damaging	1.00
IGL02197:Lama5	APN	2	179,849,012 (GRCm39)	missense	possibly damaging	0.82
IGL02308:Lama5	APN	2	179,832,120 (GRCm39)	splice site	probably benign
IGL02314:Lama5	APN	2	179,836,275 (GRCm39)	splice site	probably benign
IGL02317:Lama5	APN	2	179,833,112 (GRCm39)	missense	probably damaging	1.00
IGL02354:Lama5	APN	2	179,835,677 (GRCm39)	nonsense	probably null
IGL02361:Lama5	APN	2	179,835,677 (GRCm39)	nonsense	probably null
IGL02557:Lama5	APN	2	179,832,725 (GRCm39)	nonsense	probably null
IGL03026:Lama5	APN	2	179,837,760 (GRCm39)	missense	probably benign	0.34
IGL03160:Lama5	APN	2	179,822,128 (GRCm39)	missense	probably damaging	1.00
IGL03238:Lama5	APN	2	179,830,367 (GRCm39)	missense	probably benign
IGL03390:Lama5	APN	2	179,849,011 (GRCm39)	missense	probably damaging	1.00
blancmange	UTSW	2	179,822,404 (GRCm39)	missense	probably damaging	0.98
cupcake	UTSW	2	179,827,752 (GRCm39)	missense	probably damaging	1.00
layercake	UTSW	2	179,822,511 (GRCm39)	missense	possibly damaging	0.83
poundcake	UTSW	2	179,837,401 (GRCm39)	missense	probably damaging	1.00
Salty	UTSW	2	179,823,444 (GRCm39)	missense	possibly damaging	0.84
PIT4378001:Lama5	UTSW	2	179,831,238 (GRCm39)	missense	possibly damaging	0.89
R0003:Lama5	UTSW	2	179,819,872 (GRCm39)	splice site	probably null
R0056:Lama5	UTSW	2	179,828,899 (GRCm39)	intron	probably benign
R0147:Lama5	UTSW	2	179,832,199 (GRCm39)	missense	probably benign
R0148:Lama5	UTSW	2	179,832,199 (GRCm39)	missense	probably benign
R0310:Lama5	UTSW	2	179,823,359 (GRCm39)	splice site	probably benign
R0326:Lama5	UTSW	2	179,824,219 (GRCm39)	missense	possibly damaging	0.90
R0368:Lama5	UTSW	2	179,823,023 (GRCm39)	nonsense	probably null
R0479:Lama5	UTSW	2	179,826,250 (GRCm39)	missense	probably benign	0.03
R0490:Lama5	UTSW	2	179,821,962 (GRCm39)	missense	possibly damaging	0.90
R0636:Lama5	UTSW	2	179,831,124 (GRCm39)	critical splice donor site	probably null
R0704:Lama5	UTSW	2	179,821,277 (GRCm39)	missense	possibly damaging	0.84
R0733:Lama5	UTSW	2	179,822,511 (GRCm39)	missense	possibly damaging	0.83
R1017:Lama5	UTSW	2	179,837,213 (GRCm39)	missense	probably damaging	1.00
R1078:Lama5	UTSW	2	179,821,557 (GRCm39)	unclassified	probably benign
R1294:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1423:Lama5	UTSW	2	179,837,434 (GRCm39)	missense	probably damaging	1.00
R1438:Lama5	UTSW	2	179,824,593 (GRCm39)	missense	probably benign	0.01
R1447:Lama5	UTSW	2	179,827,671 (GRCm39)	missense	probably damaging	0.99
R1540:Lama5	UTSW	2	179,821,944 (GRCm39)	missense	probably benign
R1601:Lama5	UTSW	2	179,839,538 (GRCm39)	missense	probably damaging	1.00
R1624:Lama5	UTSW	2	179,848,551 (GRCm39)	missense	probably benign	0.02
R1674:Lama5	UTSW	2	179,843,780 (GRCm39)	missense	probably benign	0.00
R1687:Lama5	UTSW	2	179,835,859 (GRCm39)	missense	probably benign	0.00
R1696:Lama5	UTSW	2	179,844,279 (GRCm39)	missense	probably damaging	1.00
R1701:Lama5	UTSW	2	179,863,162 (GRCm39)	missense	probably damaging	1.00
R1778:Lama5	UTSW	2	179,837,274 (GRCm39)	splice site	probably benign
R1936:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1939:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1940:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1953:Lama5	UTSW	2	179,832,540 (GRCm39)	missense	possibly damaging	0.94
R1966:Lama5	UTSW	2	179,830,145 (GRCm39)	missense	probably damaging	1.00
R2024:Lama5	UTSW	2	179,820,923 (GRCm39)	missense	probably benign	0.00
R2079:Lama5	UTSW	2	179,867,301 (GRCm39)	missense	possibly damaging	0.68
R2115:Lama5	UTSW	2	179,828,678 (GRCm39)	missense	probably damaging	1.00
R2173:Lama5	UTSW	2	179,838,035 (GRCm39)	missense	probably benign	0.00
R2272:Lama5	UTSW	2	179,820,396 (GRCm39)	missense	possibly damaging	0.93
R2357:Lama5	UTSW	2	179,821,890 (GRCm39)	missense	probably benign	0.01
R2860:Lama5	UTSW	2	179,829,040 (GRCm39)	missense	probably benign	0.00
R2861:Lama5	UTSW	2	179,829,040 (GRCm39)	missense	probably benign	0.00
R2939:Lama5	UTSW	2	179,840,747 (GRCm39)	missense	probably damaging	1.00
R3053:Lama5	UTSW	2	179,824,860 (GRCm39)	missense	probably damaging	0.99
R3430:Lama5	UTSW	2	179,838,110 (GRCm39)	missense	probably benign	0.00
R3752:Lama5	UTSW	2	179,829,015 (GRCm39)	missense	probably damaging	1.00
R3782:Lama5	UTSW	2	179,836,356 (GRCm39)	missense	possibly damaging	0.57
R3901:Lama5	UTSW	2	179,824,144 (GRCm39)	splice site	probably benign
R4248:Lama5	UTSW	2	179,822,220 (GRCm39)	missense	possibly damaging	0.84
R4626:Lama5	UTSW	2	179,826,253 (GRCm39)	missense	probably damaging	0.98
R4638:Lama5	UTSW	2	179,832,206 (GRCm39)	missense	possibly damaging	0.89
R4669:Lama5	UTSW	2	179,822,430 (GRCm39)	missense	probably damaging	1.00
R4673:Lama5	UTSW	2	179,841,059 (GRCm39)	missense	probably damaging	1.00
R4677:Lama5	UTSW	2	179,821,159 (GRCm39)	missense	possibly damaging	0.69
R4701:Lama5	UTSW	2	179,833,489 (GRCm39)	missense	probably damaging	1.00
R4774:Lama5	UTSW	2	179,827,734 (GRCm39)	missense	probably damaging	1.00
R4880:Lama5	UTSW	2	179,818,861 (GRCm39)	unclassified	probably benign
R4923:Lama5	UTSW	2	179,825,942 (GRCm39)	missense	probably benign	0.18
R4960:Lama5	UTSW	2	179,850,045 (GRCm39)	critical splice donor site	probably null
R4983:Lama5	UTSW	2	179,835,242 (GRCm39)	missense	probably benign	0.13
R5061:Lama5	UTSW	2	179,840,579 (GRCm39)	nonsense	probably null
R5080:Lama5	UTSW	2	179,848,993 (GRCm39)	nonsense	probably null
R5135:Lama5	UTSW	2	179,844,013 (GRCm39)	missense	possibly damaging	0.89
R5206:Lama5	UTSW	2	179,833,097 (GRCm39)	missense	probably damaging	1.00
R5296:Lama5	UTSW	2	179,835,594 (GRCm39)	missense	probably damaging	1.00
R5319:Lama5	UTSW	2	179,822,911 (GRCm39)	missense	probably damaging	1.00
R5355:Lama5	UTSW	2	179,823,444 (GRCm39)	missense	possibly damaging	0.84
R5388:Lama5	UTSW	2	179,832,539 (GRCm39)	missense	possibly damaging	0.83
R5528:Lama5	UTSW	2	179,836,356 (GRCm39)	missense	probably benign	0.21
R5536:Lama5	UTSW	2	179,831,142 (GRCm39)	missense	probably damaging	0.99
R5658:Lama5	UTSW	2	179,850,069 (GRCm39)	nonsense	probably null
R5823:Lama5	UTSW	2	179,834,285 (GRCm39)	missense	probably benign	0.04
R5885:Lama5	UTSW	2	179,843,624 (GRCm39)	missense	probably damaging	1.00
R5889:Lama5	UTSW	2	179,835,467 (GRCm39)	intron	probably benign
R5912:Lama5	UTSW	2	179,837,268 (GRCm39)	missense	probably damaging	1.00
R5955:Lama5	UTSW	2	179,839,267 (GRCm39)	missense	probably damaging	1.00
R6015:Lama5	UTSW	2	179,827,185 (GRCm39)	missense	probably benign	0.36
R6037:Lama5	UTSW	2	179,848,806 (GRCm39)	missense	probably damaging	1.00
R6037:Lama5	UTSW	2	179,848,806 (GRCm39)	missense	probably damaging	1.00
R6191:Lama5	UTSW	2	179,827,752 (GRCm39)	missense	probably damaging	1.00
R6191:Lama5	UTSW	2	179,822,404 (GRCm39)	missense	probably damaging	0.98
R6359:Lama5	UTSW	2	179,837,775 (GRCm39)	missense	probably benign	0.01
R6385:Lama5	UTSW	2	179,838,326 (GRCm39)	missense	probably damaging	1.00
R6406:Lama5	UTSW	2	179,839,257 (GRCm39)	nonsense	probably null
R6552:Lama5	UTSW	2	179,822,947 (GRCm39)	missense	probably damaging	0.98
R6632:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6633:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6645:Lama5	UTSW	2	179,821,463 (GRCm39)	missense	probably damaging	1.00
R6731:Lama5	UTSW	2	179,830,367 (GRCm39)	missense	probably benign	0.09
R6744:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6798:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6799:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6801:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6851:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6869:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6881:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6882:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6884:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R7022:Lama5	UTSW	2	179,822,524 (GRCm39)	missense	probably damaging	1.00
R7204:Lama5	UTSW	2	179,843,970 (GRCm39)	missense	probably damaging	1.00
R7207:Lama5	UTSW	2	179,848,877 (GRCm39)	missense	probably damaging	0.98
R7282:Lama5	UTSW	2	179,843,588 (GRCm39)	missense	probably damaging	1.00
R7367:Lama5	UTSW	2	179,834,751 (GRCm39)	missense	probably benign	0.01
R7410:Lama5	UTSW	2	179,844,183 (GRCm39)	critical splice donor site	probably null
R7699:Lama5	UTSW	2	179,822,654 (GRCm39)	missense	probably damaging	1.00
R7849:Lama5	UTSW	2	179,843,605 (GRCm39)	missense	probably damaging	1.00
R7909:Lama5	UTSW	2	179,834,069 (GRCm39)	missense	possibly damaging	0.95
R7948:Lama5	UTSW	2	179,843,994 (GRCm39)	missense	probably damaging	1.00
R8153:Lama5	UTSW	2	179,829,724 (GRCm39)	missense	probably benign	0.37
R8317:Lama5	UTSW	2	179,848,784 (GRCm39)	missense	probably damaging	1.00
R8351:Lama5	UTSW	2	179,837,401 (GRCm39)	missense	probably damaging	1.00
R8370:Lama5	UTSW	2	179,843,280 (GRCm39)	missense	possibly damaging	0.80
R8398:Lama5	UTSW	2	179,838,827 (GRCm39)	critical splice donor site	probably null
R8401:Lama5	UTSW	2	179,840,580 (GRCm39)	missense	probably damaging	1.00
R8404:Lama5	UTSW	2	179,837,015 (GRCm39)	missense	probably damaging	1.00
R8502:Lama5	UTSW	2	179,837,015 (GRCm39)	missense	probably damaging	1.00
R8694:Lama5	UTSW	2	179,822,677 (GRCm39)	missense	probably damaging	0.98
R8705:Lama5	UTSW	2	179,820,354 (GRCm39)	missense	probably damaging	1.00
R8732:Lama5	UTSW	2	179,828,481 (GRCm39)	missense	probably damaging	1.00
R8755:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R8786:Lama5	UTSW	2	179,838,100 (GRCm39)	missense	probably damaging	1.00
R8926:Lama5	UTSW	2	179,835,783 (GRCm39)	missense	probably benign	0.08
R8928:Lama5	UTSW	2	179,843,832 (GRCm39)	missense	probably damaging	1.00
R8953:Lama5	UTSW	2	179,835,313 (GRCm39)	missense	probably damaging	0.99
R8958:Lama5	UTSW	2	179,835,592 (GRCm39)	missense	probably benign
R9002:Lama5	UTSW	2	179,838,311 (GRCm39)	missense	probably damaging	1.00
R9081:Lama5	UTSW	2	179,833,930 (GRCm39)	nonsense	probably null
R9165:Lama5	UTSW	2	179,821,286 (GRCm39)	missense	probably damaging	0.99
R9233:Lama5	UTSW	2	179,840,502 (GRCm39)	nonsense	probably null
R9264:Lama5	UTSW	2	179,838,271 (GRCm39)	splice site	probably benign
R9443:Lama5	UTSW	2	179,843,522 (GRCm39)	missense	probably benign	0.00
R9488:Lama5	UTSW	2	179,823,234 (GRCm39)	missense	possibly damaging	0.95
R9674:Lama5	UTSW	2	179,840,267 (GRCm39)	critical splice donor site	probably null
R9684:Lama5	UTSW	2	179,849,038 (GRCm39)	missense	probably damaging	1.00
R9749:Lama5	UTSW	2	179,825,433 (GRCm39)	missense	probably benign	0.00
RF020:Lama5	UTSW	2	179,837,971 (GRCm39)	missense	probably benign
X0065:Lama5	UTSW	2	179,823,524 (GRCm39)	missense	probably benign	0.26
Z1177:Lama5	UTSW	2	179,832,507 (GRCm39)	missense	possibly damaging	0.95
Z1177:Lama5	UTSW	2	179,831,212 (GRCm39)	missense	probably damaging	1.00
Z1177:Lama5	UTSW	2	179,825,423 (GRCm39)	missense	probably benign	0.03
Z1177:Lama5	UTSW	2	179,840,603 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGCAAGTAGTGGTCCTTCG -3'
(R):5'- GCAGACCTGAAGAGTTGATGTTAG -3'

Sequencing Primer
(F):5'- GCTGGCCTGCCATGAAACAG -3'
(R):5'- CTATGGGGCCTAACCAGGAG -3'

Posted On

2022-03-25