Mutagenetix > Incidental Mutation

Incidental Mutation 'R9353:Muc21'

708237

Institutional Source

Beutler Lab

Gene Symbol

Muc21

Ensembl Gene

ENSMUSG00000090588

Gene Name

mucin 21

Synonyms

epiglycanin, Gm9573

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R9353 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

35928815-35937529 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 35930545 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 1214 (T1214A)

Ref Sequence

ENSEMBL: ENSMUSP00000130987 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164502] [ENSMUST00000174521]

AlphaFold

F7C950

Predicted Effect

unknown
Transcript: ENSMUST00000164502
AA Change: T1214A

SMART Domains

Protein: ENSMUSP00000130987
Gene: ENSMUSG00000090588
AA Change: T1214A

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
low complexity region	26	39	N/A	INTRINSIC
low complexity region	42	59	N/A	INTRINSIC
low complexity region	76	144	N/A	INTRINSIC
low complexity region	149	578	N/A	INTRINSIC
low complexity region	580	653	N/A	INTRINSIC
low complexity region	655	1179	N/A	INTRINSIC
low complexity region	1183	1373	N/A	INTRINSIC
low complexity region	1383	1436	N/A	INTRINSIC
low complexity region	1438	1479	N/A	INTRINSIC
Pfam:Epiglycanin_C	1518	1605	3.8e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000174521

SMART Domains

Protein: ENSMUSP00000134221
Gene: ENSMUSG00000090509

Domain	Start	End	E-Value	Type
Pfam:SFTA2	80	117	9.2e-18	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

98% (44/45)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atp7b	A	T	8: 22,517,890 (GRCm39)	V316D	possibly damaging	Het
Bhmt1b	A	G	18: 87,774,954 (GRCm39)	E159G	probably damaging	Het
Cc2d2a	T	C	5: 43,860,691 (GRCm39)		probably null	Het
Ccdc88a	A	G	11: 29,427,433 (GRCm39)	D1046G	probably damaging	Het
Ccl12	A	T	11: 81,993,437 (GRCm39)	D25V	possibly damaging	Het
Cdh23	G	A	10: 60,143,306 (GRCm39)	A3005V	possibly damaging	Het
Ces3a	G	T	8: 105,776,547 (GRCm39)	R45L	probably benign	Het
Cntln	G	T	4: 84,802,597 (GRCm39)		probably benign	Het
Crybg3	C	A	16: 59,421,107 (GRCm39)		probably null	Het
Cxxc4	G	T	3: 133,945,913 (GRCm39)	G165C	unknown	Het
Dab2ip	G	A	2: 35,598,851 (GRCm39)	C181Y	probably damaging	Het
Dnah11	A	G	12: 118,143,434 (GRCm39)	V403A	probably benign	Het
Dnah12	T	C	14: 26,578,507 (GRCm39)	S3089P	probably damaging	Het
Dnajc13	A	T	9: 104,067,571 (GRCm39)	I1196N	probably benign	Het
Faiml	T	C	9: 99,116,462 (GRCm39)	Y76C	probably damaging	Het
Fam171b	T	A	2: 83,707,028 (GRCm39)	H299Q	probably benign	Het
Fbxo36	A	G	1: 84,874,259 (GRCm39)	N85S	probably benign	Het
Filip1	A	G	9: 79,725,623 (GRCm39)	F999L	possibly damaging	Het
Gtf3c3	A	T	1: 54,445,211 (GRCm39)	S614R	possibly damaging	Het
Il18rap	A	G	1: 40,587,088 (GRCm39)	T457A	probably benign	Het
Inmt	C	T	6: 55,151,984 (GRCm39)		probably benign	Het
Kcnb1	C	T	2: 166,947,007 (GRCm39)	G614S	probably benign	Het
Kdm2a	C	T	19: 4,393,141 (GRCm39)	D405N		Het
Kdm5a	T	A	6: 120,404,730 (GRCm39)	V1324E	probably benign	Het
Lyn	A	T	4: 3,746,804 (GRCm39)	Y194F	possibly damaging	Het
Mdn1	A	G	4: 32,693,504 (GRCm39)	D1043G	probably damaging	Het
Mier1	T	A	4: 103,012,800 (GRCm39)	H397Q	probably damaging	Het
Mov10l1	T	A	15: 88,872,622 (GRCm39)	D105E	possibly damaging	Het
Nav3	A	G	10: 109,554,065 (GRCm39)	S1766P	probably damaging	Het
Ncdn	T	A	4: 126,644,464 (GRCm39)	E119D	probably benign	Het
Nckipsd	C	A	9: 108,691,471 (GRCm39)	A416E	probably damaging	Het
Nek5	A	G	8: 22,563,961 (GRCm39)	V623A	probably benign	Het
Oas1g	A	G	5: 121,023,986 (GRCm39)	Y108H	possibly damaging	Het
Or13n4	T	C	7: 106,423,062 (GRCm39)	T224A	probably benign	Het
P2ry1	T	C	3: 60,911,916 (GRCm39)	S352P	probably damaging	Het
Pde6a	T	C	18: 61,390,382 (GRCm39)	F535S	probably damaging	Het
Pitpnb	T	C	5: 111,530,891 (GRCm39)	L228P	probably damaging	Het
Rbck1	G	T	2: 152,161,145 (GRCm39)	H368N	probably damaging	Het
Snrnp35	T	A	5: 124,628,559 (GRCm39)	V124E	probably damaging	Het
Spdye4a	C	T	5: 143,204,793 (GRCm39)	M224I	probably benign	Het
Stau1	A	G	2: 166,792,267 (GRCm39)	Y424H	probably damaging	Het
Sult1c2	A	T	17: 54,271,060 (GRCm39)	D190E	probably benign	Het
Thbs1	A	G	2: 117,953,051 (GRCm39)	D887G	probably damaging	Het
Tiam2	C	T	17: 3,558,074 (GRCm39)	Q1233*	probably null	Het
Tmem88	A	G	11: 69,288,939 (GRCm39)	V38A	probably damaging	Het
Vmn1r219	G	A	13: 23,346,902 (GRCm39)	M30I	probably benign	Het
Zswim3	A	G	2: 164,662,261 (GRCm39)	H247R	probably damaging	Het

Other mutations in Muc21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
BB009:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
FR4304:Muc21	UTSW	17	35,933,013 (GRCm39)	intron	probably benign
R0334:Muc21	UTSW	17	35,933,614 (GRCm39)	intron	probably benign
R0946:Muc21	UTSW	17	35,929,105 (GRCm39)	missense	probably benign	0.32
R1117:Muc21	UTSW	17	35,930,920 (GRCm39)	intron	probably benign
R1345:Muc21	UTSW	17	35,932,489 (GRCm39)	intron	probably benign
R1697:Muc21	UTSW	17	35,931,540 (GRCm39)	intron	probably benign
R1750:Muc21	UTSW	17	35,931,940 (GRCm39)	intron	probably benign
R1756:Muc21	UTSW	17	35,930,131 (GRCm39)	intron	probably benign
R1946:Muc21	UTSW	17	35,933,416 (GRCm39)	intron	probably benign
R1978:Muc21	UTSW	17	35,933,857 (GRCm39)	intron	probably benign
R1991:Muc21	UTSW	17	35,929,600 (GRCm39)	missense	probably benign	0.32
R1992:Muc21	UTSW	17	35,929,600 (GRCm39)	missense	probably benign	0.32
R2063:Muc21	UTSW	17	35,932,297 (GRCm39)	intron	probably benign
R2356:Muc21	UTSW	17	35,932,563 (GRCm39)	intron	probably benign
R2866:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R3826:Muc21	UTSW	17	35,932,504 (GRCm39)	intron	probably benign
R4020:Muc21	UTSW	17	35,930,953 (GRCm39)	intron	probably benign
R4474:Muc21	UTSW	17	35,931,496 (GRCm39)	intron	probably benign
R4677:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R4786:Muc21	UTSW	17	35,930,221 (GRCm39)	intron	probably benign
R5071:Muc21	UTSW	17	35,931,444 (GRCm39)	intron	probably benign
R5173:Muc21	UTSW	17	35,931,633 (GRCm39)	intron	probably benign
R5283:Muc21	UTSW	17	35,932,224 (GRCm39)	intron	probably benign
R5446:Muc21	UTSW	17	35,933,395 (GRCm39)	intron	probably benign
R5542:Muc21	UTSW	17	35,933,395 (GRCm39)	intron	probably benign
R5716:Muc21	UTSW	17	35,931,675 (GRCm39)	intron	probably benign
R5913:Muc21	UTSW	17	35,934,123 (GRCm39)	intron	probably benign
R6011:Muc21	UTSW	17	35,933,074 (GRCm39)	intron	probably benign
R6198:Muc21	UTSW	17	35,931,808 (GRCm39)	intron	probably benign
R6394:Muc21	UTSW	17	35,931,058 (GRCm39)	intron	probably benign
R6786:Muc21	UTSW	17	35,934,057 (GRCm39)	intron	probably benign
R6940:Muc21	UTSW	17	35,934,118 (GRCm39)	intron	probably benign
R7082:Muc21	UTSW	17	35,932,093 (GRCm39)	missense	unknown
R7103:Muc21	UTSW	17	35,932,432 (GRCm39)	missense	unknown
R7110:Muc21	UTSW	17	35,933,510 (GRCm39)	intron	probably benign
R7139:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R7165:Muc21	UTSW	17	35,932,870 (GRCm39)	missense	unknown
R7200:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R7204:Muc21	UTSW	17	35,932,105 (GRCm39)	intron	probably benign
R7289:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7290:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7295:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7319:Muc21	UTSW	17	35,932,935 (GRCm39)	intron	probably benign
R7462:Muc21	UTSW	17	35,931,568 (GRCm39)	missense	unknown
R7529:Muc21	UTSW	17	35,930,123 (GRCm39)	missense	unknown
R7718:Muc21	UTSW	17	35,933,728 (GRCm39)	missense	unknown
R7762:Muc21	UTSW	17	35,932,977 (GRCm39)	missense	unknown
R7788:Muc21	UTSW	17	35,929,798 (GRCm39)	missense	unknown
R7798:Muc21	UTSW	17	35,932,146 (GRCm39)	missense	unknown
R7831:Muc21	UTSW	17	35,929,651 (GRCm39)	missense	unknown
R7896:Muc21	UTSW	17	35,930,917 (GRCm39)	missense	unknown
R7899:Muc21	UTSW	17	35,931,493 (GRCm39)	intron	probably benign
R7932:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R8025:Muc21	UTSW	17	35,931,879 (GRCm39)	intron	probably benign
R8077:Muc21	UTSW	17	35,930,628 (GRCm39)	intron	probably benign
R8090:Muc21	UTSW	17	35,932,617 (GRCm39)	missense	unknown
R8169:Muc21	UTSW	17	35,932,072 (GRCm39)	missense	unknown
R8184:Muc21	UTSW	17	35,933,722 (GRCm39)	missense	unknown
R8209:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R8226:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R8464:Muc21	UTSW	17	35,933,098 (GRCm39)	intron	probably benign
R8670:Muc21	UTSW	17	35,932,540 (GRCm39)	missense	unknown
R8783:Muc21	UTSW	17	35,930,875 (GRCm39)	missense	unknown
R8856:Muc21	UTSW	17	35,931,865 (GRCm39)	missense	unknown
R9155:Muc21	UTSW	17	35,932,131 (GRCm39)	missense	unknown
R9214:Muc21	UTSW	17	35,931,838 (GRCm39)	missense	unknown
R9618:Muc21	UTSW	17	35,932,935 (GRCm39)	intron	probably benign
R9621:Muc21	UTSW	17	35,932,720 (GRCm39)	missense	unknown
R9679:Muc21	UTSW	17	35,930,491 (GRCm39)	missense	unknown
RF025:Muc21	UTSW	17	35,933,771 (GRCm39)	intron	probably benign
Z1176:Muc21	UTSW	17	35,932,137 (GRCm39)	missense	unknown
Z1177:Muc21	UTSW	17	35,931,951 (GRCm39)	missense	unknown
Z1177:Muc21	UTSW	17	35,931,817 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- ACCTGAGGCAATGCTTGATGC -3'
(R):5'- TGTATCCAGCACTGCCTCAG -3'

Sequencing Primer
(F):5'- CTGAGGCAGTGCTAGATGC -3'
(R):5'- AGGCTCTACACCTACCCTG -3'

Posted On

2022-04-18