Mutagenetix > Incidental Mutation

Incidental Mutation 'R8464:Muc21'

656727

Institutional Source

Beutler Lab

Gene Symbol

Muc21

Ensembl Gene

ENSMUSG00000090588

Gene Name

mucin 21

Synonyms

epiglycanin, Gm9573

MMRRC Submission

067908-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R8464 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

35928815-35937529 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

T to G at 35933098 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000134221 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164502] [ENSMUST00000174521]

AlphaFold

F7C950

Predicted Effect

unknown
Transcript: ENSMUST00000164502
AA Change: S363R

SMART Domains

Protein: ENSMUSP00000130987
Gene: ENSMUSG00000090588
AA Change: S363R

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
low complexity region	26	39	N/A	INTRINSIC
low complexity region	42	59	N/A	INTRINSIC
low complexity region	76	144	N/A	INTRINSIC
low complexity region	149	578	N/A	INTRINSIC
low complexity region	580	653	N/A	INTRINSIC
low complexity region	655	1179	N/A	INTRINSIC
low complexity region	1183	1373	N/A	INTRINSIC
low complexity region	1383	1436	N/A	INTRINSIC
low complexity region	1438	1479	N/A	INTRINSIC
Pfam:Epiglycanin_C	1518	1605	3.8e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000174521

SMART Domains

Protein: ENSMUSP00000134221
Gene: ENSMUSG00000090509

Domain	Start	End	E-Value	Type
Pfam:SFTA2	80	117	9.2e-18	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	T	C	14: 32,381,934 (GRCm39)	R1344G	possibly damaging	Het
4933402J07Rik	C	A	8: 88,315,649 (GRCm39)	D246E	probably damaging	Het
Appl1	T	A	14: 26,674,985 (GRCm39)	K191*	probably null	Het
Bptf	T	C	11: 107,022,168 (GRCm39)	D194G	probably benign	Het
Cacna2d2	T	C	9: 107,389,206 (GRCm39)	V223A	probably damaging	Het
Ccdc13	T	C	9: 121,649,824 (GRCm39)	K208E	probably damaging	Het
Chd7	T	C	4: 8,811,465 (GRCm39)	V813A	probably benign	Het
Chst14	T	C	2: 118,757,524 (GRCm39)	V131A	probably benign	Het
Ctla4	A	G	1: 60,951,686 (GRCm39)	T72A	probably damaging	Het
Dnmt3a	T	C	12: 3,949,635 (GRCm39)	S531P	probably benign	Het
Erbb4	T	C	1: 68,348,785 (GRCm39)	I531V	probably benign	Het
Fam151a	T	A	4: 106,605,102 (GRCm39)	M488K	probably benign	Het
Foxa1	C	A	12: 57,589,246 (GRCm39)	A325S	probably benign	Het
Ighv8-5	C	T	12: 115,031,309 (GRCm39)	D77N	probably benign	Het
Itga3	C	A	11: 94,953,566 (GRCm39)	A259S	probably benign	Het
Kcnh4	T	C	11: 100,648,010 (GRCm39)	N118D	probably damaging	Het
Kif5b	A	T	18: 6,225,381 (GRCm39)	N216K	probably damaging	Het
Lipo2	C	T	19: 33,726,023 (GRCm39)	M76I	probably benign	Het
Man2b1	G	A	8: 85,820,772 (GRCm39)	A657T	possibly damaging	Het
Mms19	A	C	19: 41,935,522 (GRCm39)	D831E	probably damaging	Het
Myo1a	G	T	10: 127,554,453 (GRCm39)	A808S	probably benign	Het
Myo7b	A	T	18: 32,095,757 (GRCm39)	W1834R	probably benign	Het
Mypn	T	A	10: 62,966,977 (GRCm39)	K900*	probably null	Het
Ogfr	AGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCAAAAGGCCAGGTGGAGCCAGAGGACCCAAAAGGCCAGGTGGGGCCAGAAGACCCAAAAGGCCAGGTGGGGCCAGAGGACCCAAAAGGCCAGGTGGGGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCCAAAGCCAGGTGGGGCCAGAG	AGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCAAAAGGCCAGGTGGAGCCAGAGGACCCAAAAGGCCAGGTGGGGCCAGAAGACCCAAAAGGCCAGGTGGGGCCAGAGGACCCAAAAGGCCAGGTGGGGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCCAAAGCCAGGTGGGGCCAGAG	2: 180,236,850 (GRCm39)		probably benign	Het
Or4b1d	G	T	2: 89,968,947 (GRCm39)	H179N	possibly damaging	Het
Or4k40	A	G	2: 111,251,192 (GRCm39)	Y35H	probably damaging	Het
Or6c212	A	T	10: 129,558,783 (GRCm39)	I210N	possibly damaging	Het
Osgepl1	A	G	1: 53,357,299 (GRCm39)	T154A	probably damaging	Het
Pacsin2	A	T	15: 83,263,384 (GRCm39)	Y82*	probably null	Het
Pomgnt1	T	C	4: 116,009,348 (GRCm39)	Y104H	probably damaging	Het
Robo3	T	C	9: 37,332,726 (GRCm39)	S857G	probably damaging	Het
Scn9a	T	C	2: 66,396,625 (GRCm39)	I89M	probably damaging	Het
Sdhd	T	C	9: 50,508,431 (GRCm39)	H145R	probably benign	Het
Selenov	C	T	7: 27,987,897 (GRCm39)	R260Q	probably benign	Het
Spag6l	T	C	16: 16,580,898 (GRCm39)	E483G	probably damaging	Het
Ssh2	T	A	11: 77,345,079 (GRCm39)	Y1021*	probably null	Het
Syne2	C	T	12: 76,012,546 (GRCm39)	A2580V	probably benign	Het
Taf6	T	C	5: 138,180,924 (GRCm39)	E219G	probably damaging	Het
Ttc27	A	G	17: 75,024,925 (GRCm39)	T7A	probably benign	Het
Unc80	A	G	1: 66,512,423 (GRCm39)	K111R	probably damaging	Het

Other mutations in Muc21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
BB009:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
FR4304:Muc21	UTSW	17	35,933,013 (GRCm39)	intron	probably benign
R0334:Muc21	UTSW	17	35,933,614 (GRCm39)	intron	probably benign
R0946:Muc21	UTSW	17	35,929,105 (GRCm39)	missense	probably benign	0.32
R1117:Muc21	UTSW	17	35,930,920 (GRCm39)	intron	probably benign
R1345:Muc21	UTSW	17	35,932,489 (GRCm39)	intron	probably benign
R1697:Muc21	UTSW	17	35,931,540 (GRCm39)	intron	probably benign
R1750:Muc21	UTSW	17	35,931,940 (GRCm39)	intron	probably benign
R1756:Muc21	UTSW	17	35,930,131 (GRCm39)	intron	probably benign
R1946:Muc21	UTSW	17	35,933,416 (GRCm39)	intron	probably benign
R1978:Muc21	UTSW	17	35,933,857 (GRCm39)	intron	probably benign
R1991:Muc21	UTSW	17	35,929,600 (GRCm39)	missense	probably benign	0.32
R1992:Muc21	UTSW	17	35,929,600 (GRCm39)	missense	probably benign	0.32
R2063:Muc21	UTSW	17	35,932,297 (GRCm39)	intron	probably benign
R2356:Muc21	UTSW	17	35,932,563 (GRCm39)	intron	probably benign
R2866:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R3826:Muc21	UTSW	17	35,932,504 (GRCm39)	intron	probably benign
R4020:Muc21	UTSW	17	35,930,953 (GRCm39)	intron	probably benign
R4474:Muc21	UTSW	17	35,931,496 (GRCm39)	intron	probably benign
R4677:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R4786:Muc21	UTSW	17	35,930,221 (GRCm39)	intron	probably benign
R5071:Muc21	UTSW	17	35,931,444 (GRCm39)	intron	probably benign
R5173:Muc21	UTSW	17	35,931,633 (GRCm39)	intron	probably benign
R5283:Muc21	UTSW	17	35,932,224 (GRCm39)	intron	probably benign
R5446:Muc21	UTSW	17	35,933,395 (GRCm39)	intron	probably benign
R5542:Muc21	UTSW	17	35,933,395 (GRCm39)	intron	probably benign
R5716:Muc21	UTSW	17	35,931,675 (GRCm39)	intron	probably benign
R5913:Muc21	UTSW	17	35,934,123 (GRCm39)	intron	probably benign
R6011:Muc21	UTSW	17	35,933,074 (GRCm39)	intron	probably benign
R6198:Muc21	UTSW	17	35,931,808 (GRCm39)	intron	probably benign
R6394:Muc21	UTSW	17	35,931,058 (GRCm39)	intron	probably benign
R6786:Muc21	UTSW	17	35,934,057 (GRCm39)	intron	probably benign
R6940:Muc21	UTSW	17	35,934,118 (GRCm39)	intron	probably benign
R7082:Muc21	UTSW	17	35,932,093 (GRCm39)	missense	unknown
R7103:Muc21	UTSW	17	35,932,432 (GRCm39)	missense	unknown
R7110:Muc21	UTSW	17	35,933,510 (GRCm39)	intron	probably benign
R7139:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R7165:Muc21	UTSW	17	35,932,870 (GRCm39)	missense	unknown
R7200:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R7204:Muc21	UTSW	17	35,932,105 (GRCm39)	intron	probably benign
R7289:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7290:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7295:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7319:Muc21	UTSW	17	35,932,935 (GRCm39)	intron	probably benign
R7462:Muc21	UTSW	17	35,931,568 (GRCm39)	missense	unknown
R7529:Muc21	UTSW	17	35,930,123 (GRCm39)	missense	unknown
R7718:Muc21	UTSW	17	35,933,728 (GRCm39)	missense	unknown
R7762:Muc21	UTSW	17	35,932,977 (GRCm39)	missense	unknown
R7788:Muc21	UTSW	17	35,929,798 (GRCm39)	missense	unknown
R7798:Muc21	UTSW	17	35,932,146 (GRCm39)	missense	unknown
R7831:Muc21	UTSW	17	35,929,651 (GRCm39)	missense	unknown
R7896:Muc21	UTSW	17	35,930,917 (GRCm39)	missense	unknown
R7899:Muc21	UTSW	17	35,931,493 (GRCm39)	intron	probably benign
R7932:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R8025:Muc21	UTSW	17	35,931,879 (GRCm39)	intron	probably benign
R8077:Muc21	UTSW	17	35,930,628 (GRCm39)	intron	probably benign
R8090:Muc21	UTSW	17	35,932,617 (GRCm39)	missense	unknown
R8169:Muc21	UTSW	17	35,932,072 (GRCm39)	missense	unknown
R8184:Muc21	UTSW	17	35,933,722 (GRCm39)	missense	unknown
R8209:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R8226:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R8670:Muc21	UTSW	17	35,932,540 (GRCm39)	missense	unknown
R8783:Muc21	UTSW	17	35,930,875 (GRCm39)	missense	unknown
R8856:Muc21	UTSW	17	35,931,865 (GRCm39)	missense	unknown
R9155:Muc21	UTSW	17	35,932,131 (GRCm39)	missense	unknown
R9214:Muc21	UTSW	17	35,931,838 (GRCm39)	missense	unknown
R9353:Muc21	UTSW	17	35,930,545 (GRCm39)	missense	unknown
R9618:Muc21	UTSW	17	35,932,935 (GRCm39)	intron	probably benign
R9621:Muc21	UTSW	17	35,932,720 (GRCm39)	missense	unknown
R9679:Muc21	UTSW	17	35,930,491 (GRCm39)	missense	unknown
RF025:Muc21	UTSW	17	35,933,771 (GRCm39)	intron	probably benign
Z1176:Muc21	UTSW	17	35,932,137 (GRCm39)	missense	unknown
Z1177:Muc21	UTSW	17	35,931,951 (GRCm39)	missense	unknown
Z1177:Muc21	UTSW	17	35,931,817 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- ATAGATCCTGAGGCAGAGCTG -3'
(R):5'- GCACTGTCTCAGACTCTACACC -3'

Sequencing Primer
(F):5'- CCTGAGGCAGTGCTGGATG -3'
(R):5'- GCATCTAGCACTGCCTCAGGATC -3'

Posted On

2021-01-18