Mutagenetix > Incidental Mutation

Incidental Mutation 'R9358:Vmn2r32'

708523

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r32

Ensembl Gene

ENSMUSG00000096743

Gene Name

vomeronasal 2, receptor 32

Synonyms

V2r5

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.249) question?

Stock #

R9358 (G1)

Quality Score

218.009

Status

Not validated

Chromosome

Chromosomal Location

7466968-7482972 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 7477197 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Lysine at position 398 (I398K)

Ref Sequence

ENSEMBL: ENSMUSP00000092462 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094866]

AlphaFold

K7N686

Predicted Effect

probably damaging
Transcript: ENSMUST00000094866
AA Change: I398K

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000092462
Gene: ENSMUSG00000096743
AA Change: I398K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	470	3.6e-33	PFAM
Pfam:NCD3G	512	565	8.1e-21	PFAM
Pfam:7tm_3	598	833	9.5e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930432M17Rik	A	T	3: 121,474,976 (GRCm39)	K149*	probably null	Het
Ap3m2	G	A	8: 23,280,959 (GRCm39)	S365F	possibly damaging	Het
Apob	T	A	12: 8,060,833 (GRCm39)	I3105K	probably benign	Het
Arfgef3	A	G	10: 18,492,628 (GRCm39)	L1261P	probably damaging	Het
Armc8	A	G	9: 99,450,653 (GRCm39)		probably null	Het
Axin2	A	G	11: 108,814,873 (GRCm39)	T254A	probably benign	Het
Cachd1	G	A	4: 100,833,622 (GRCm39)	V800M	probably damaging	Het
Camta1	A	G	4: 151,222,881 (GRCm39)	L892P	probably damaging	Het
Cfap58	C	T	19: 47,962,987 (GRCm39)	R466*	probably null	Het
Cilp	A	G	9: 65,183,269 (GRCm39)	Q391R	probably benign	Het
Cracd	A	T	5: 77,002,836 (GRCm39)	I137F	probably damaging	Het
Ctcfl	T	A	2: 172,960,581 (GRCm39)	M1L	possibly damaging	Het
Dnah14	G	A	1: 181,536,598 (GRCm39)	A2414T	probably benign	Het
Dnah2	A	G	11: 69,406,592 (GRCm39)	L550P	probably damaging	Het
Erich6b	T	A	14: 75,902,668 (GRCm39)	S162T	probably benign	Het
Fbxw7	C	A	3: 84,883,561 (GRCm39)	H578Q		Het
Fer	T	C	17: 64,280,076 (GRCm39)	L418P	possibly damaging	Het
Fndc3b	A	C	3: 27,505,556 (GRCm39)	L904R	possibly damaging	Het
Gabrg1	A	T	5: 70,935,422 (GRCm39)	I249N	possibly damaging	Het
Gbe1	T	A	16: 70,238,127 (GRCm39)	D304E	probably benign	Het
Gm10549	C	T	18: 33,597,375 (GRCm39)	P54S	unknown	Het
Gpatch4	G	T	3: 87,962,452 (GRCm39)	E222*	probably null	Het
Heatr1	A	T	13: 12,433,087 (GRCm39)	T1146S	probably benign	Het
Hrc	A	C	7: 44,985,984 (GRCm39)	E378D	probably benign	Het
Hspg2	G	T	4: 137,244,909 (GRCm39)	R878L	probably damaging	Het
Igkv1-122	A	G	6: 67,993,756 (GRCm39)	M11V	probably benign	Het
Itih3	T	A	14: 30,643,885 (GRCm39)	K75*	probably null	Het
Jag1	A	G	2: 136,924,948 (GRCm39)	V1218A	probably benign	Het
Kif28	C	T	1: 179,563,695 (GRCm39)	D94N	probably benign	Het
Lama2	G	A	10: 27,064,378 (GRCm39)	T1201M	possibly damaging	Het
Lama2	A	G	10: 27,492,761 (GRCm39)	L11P	unknown	Het
Lrfn2	T	C	17: 49,403,530 (GRCm39)	V551A	probably damaging	Het
Lrrc8d	A	G	5: 105,960,358 (GRCm39)	N256S	probably benign	Het
Mindy1	C	T	3: 95,202,590 (GRCm39)	L394F	probably benign	Het
Mon2	A	G	10: 122,868,452 (GRCm39)	S534P	probably benign	Het
Mrps5	T	A	2: 127,437,734 (GRCm39)	I187N	probably benign	Het
Mto1	A	G	9: 78,364,840 (GRCm39)	M360V	probably benign	Het
Muc16	T	G	9: 18,553,520 (GRCm39)	I4258L	probably benign	Het
Myo10	A	G	15: 25,781,520 (GRCm39)	D1005G	possibly damaging	Het
Myo18b	T	A	5: 112,943,269 (GRCm39)	R1645S	possibly damaging	Het
Naip2	A	T	13: 100,298,080 (GRCm39)	V652D	probably damaging	Het
Naip5	T	A	13: 100,356,338 (GRCm39)	E1092D	probably benign	Het
Ncapd2	A	T	6: 125,163,106 (GRCm39)	M134K	probably benign	Het
Nup107	A	G	10: 117,586,868 (GRCm39)	F893S	probably damaging	Het
Or10j3	A	T	1: 173,031,741 (GRCm39)	R273W	probably damaging	Het
Or11h23	A	G	14: 50,947,802 (GRCm39)	E5G	probably benign	Het
Or1e35	A	T	11: 73,797,451 (GRCm39)	I289N	probably damaging	Het
Or4f14b	A	G	2: 111,775,429 (GRCm39)	V124A	probably benign	Het
Or5p64	C	T	7: 107,854,799 (GRCm39)	C182Y	probably damaging	Het
Pcgf1	G	T	6: 83,056,433 (GRCm39)	R124L	probably benign	Het
Pdcd10	A	T	3: 75,448,533 (GRCm39)	N10K	probably damaging	Het
Pdx1	C	T	5: 147,207,064 (GRCm39)	Q6*	probably null	Het
Plcl1	T	C	1: 55,735,810 (GRCm39)	S384P	probably damaging	Het
Serpina1b	T	A	12: 103,694,653 (GRCm39)	T364S	possibly damaging	Het
Slc5a11	C	T	7: 122,857,775 (GRCm39)	T288I	probably damaging	Het
Slc6a1	A	T	6: 114,291,271 (GRCm39)	S515C	probably damaging	Het
Snca	A	T	6: 60,710,121 (GRCm39)	D119E	probably benign	Het
Sorcs2	T	C	5: 36,200,814 (GRCm39)	D578G	probably damaging	Het
Sulf1	T	A	1: 12,890,729 (GRCm39)	L389Q	probably damaging	Het
Thoc2l	T	C	5: 104,667,826 (GRCm39)	F783L	possibly damaging	Het
Tln1	A	C	4: 43,532,084 (GRCm39)	I2501S	possibly damaging	Het
Tmem191	A	G	16: 17,094,257 (GRCm39)	E27G	probably damaging	Het
Tmem200a	G	T	10: 25,869,677 (GRCm39)	N197K	probably benign	Het
Tmem207	A	T	16: 26,345,434 (GRCm39)	S11T	probably benign	Het
Ttn	T	C	2: 76,564,953 (GRCm39)	T28425A	probably damaging	Het
Ush2a	C	T	1: 188,607,147 (GRCm39)	T4014M	probably benign	Het
Vac14	G	A	8: 111,439,379 (GRCm39)		probably null	Het
Vmn2r77	A	T	7: 86,452,236 (GRCm39)	H472L	probably benign	Het
Vps45	C	T	3: 95,940,976 (GRCm39)		probably null	Het
Wdr26	T	C	1: 181,019,423 (GRCm39)	H334R	probably damaging	Het
Zfp268	A	T	4: 145,349,613 (GRCm39)	Y350F	possibly damaging	Het
Zmynd10	A	G	9: 107,426,249 (GRCm39)	D132G	possibly damaging	Het
Zpbp2	A	G	11: 98,444,774 (GRCm39)	T123A	probably benign	Het

Other mutations in Vmn2r32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02140:Vmn2r32	APN	7	7,479,696 (GRCm39)	missense	probably damaging	0.97
IGL02333:Vmn2r32	APN	7	7,467,143 (GRCm39)	missense	probably damaging	1.00
IGL02406:Vmn2r32	APN	7	7,479,709 (GRCm39)	missense	probably benign
IGL02428:Vmn2r32	APN	7	7,477,283 (GRCm39)	missense	probably benign	0.02
IGL02484:Vmn2r32	APN	7	7,467,116 (GRCm39)	missense	probably damaging	1.00
IGL03277:Vmn2r32	APN	7	7,477,251 (GRCm39)	missense	probably benign	0.23
IGL03366:Vmn2r32	APN	7	7,467,029 (GRCm39)	missense	probably damaging	0.99
R1055:Vmn2r32	UTSW	7	7,477,326 (GRCm39)	nonsense	probably null
R1695:Vmn2r32	UTSW	7	7,466,991 (GRCm39)	missense	probably benign	0.01
R2172:Vmn2r32	UTSW	7	7,477,614 (GRCm39)	missense	probably damaging	0.99
R2262:Vmn2r32	UTSW	7	7,477,618 (GRCm39)	missense	probably benign
R3150:Vmn2r32	UTSW	7	7,475,554 (GRCm39)	missense	probably benign
R4362:Vmn2r32	UTSW	7	7,482,857 (GRCm39)	nonsense	probably null
R4432:Vmn2r32	UTSW	7	7,482,918 (GRCm39)	missense	probably damaging	0.98
R4851:Vmn2r32	UTSW	7	7,482,953 (GRCm39)	missense	possibly damaging	0.59
R4949:Vmn2r32	UTSW	7	7,467,083 (GRCm39)	missense	probably benign	0.22
R5990:Vmn2r32	UTSW	7	7,482,809 (GRCm39)	missense	probably damaging	0.97
R6083:Vmn2r32	UTSW	7	7,467,209 (GRCm39)	missense	probably benign	0.15
R6084:Vmn2r32	UTSW	7	7,467,209 (GRCm39)	missense	probably benign	0.15
R6116:Vmn2r32	UTSW	7	7,467,092 (GRCm39)	missense	probably damaging	1.00
R6263:Vmn2r32	UTSW	7	7,479,691 (GRCm39)	missense	possibly damaging	0.90
R6889:Vmn2r32	UTSW	7	7,475,573 (GRCm39)	missense	possibly damaging	0.82
R7286:Vmn2r32	UTSW	7	7,482,807 (GRCm39)	missense	probably benign	0.21
R7390:Vmn2r32	UTSW	7	7,482,851 (GRCm39)	missense	probably benign	0.00
R7412:Vmn2r32	UTSW	7	7,477,212 (GRCm39)	missense	possibly damaging	0.53
R7508:Vmn2r32	UTSW	7	7,470,373 (GRCm39)	missense	possibly damaging	0.87
R8812:Vmn2r32	UTSW	7	7,477,669 (GRCm39)	missense	probably damaging	1.00
R8968:Vmn2r32	UTSW	7	7,477,204 (GRCm39)	missense	probably benign	0.00
R9331:Vmn2r32	UTSW	7	7,467,402 (GRCm39)	nonsense	probably null
Z1177:Vmn2r32	UTSW	7	7,477,160 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- AGTCTGGGCATCTGAAAGACTC -3'
(R):5'- AGTCATGACACATTCTATGGATCAC -3'

Sequencing Primer
(F):5'- GTCTGGGCATCTGAAAGACTCTAAAC -3'
(R):5'- GTACAGACATGGTTCCATCTCAG -3'

Posted On

2022-04-18