Mutagenetix > Incidental Mutation

Incidental Mutation 'R1756:Ccnb2'

194867

Institutional Source

Beutler Lab

Gene Symbol

Ccnb2

Ensembl Gene

ENSMUSG00000032218

Gene Name

cyclin B2

Synonyms

CycB2

MMRRC Submission

039788-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.722) question?

Stock #

R1756 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

70407692-70421547 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 70410788 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 234 (V234A)

Ref Sequence

ENSEMBL: ENSMUSP00000034742 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034742]

AlphaFold

P30276

Predicted Effect

probably benign
Transcript: ENSMUST00000034742
AA Change: V234A

PolyPhen 2 Score 0.406 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000034742
Gene: ENSMUSG00000032218
AA Change: V234A

Domain	Start	End	E-Value	Type
CYCLIN	171	255	8.58e-28	SMART
Cyclin_C	264	382	9.83e-34	SMART
CYCLIN	268	349	2.73e-21	SMART

Meta Mutation Damage Score

0.0671

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.5%
20x: 93.0%

Validation Efficiency

100% (96/96)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutation of this gene results in reduced body weight and reduced litter size. Homozygous pups are underrepresented in litters from a heterozygous intercross. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 94 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010111I01Rik	T	A	13: 63,068,061 (GRCm38)	H382Q	possibly damaging	Het
A330008L17Rik	C	A	8: 99,421,882 (GRCm38)		noncoding transcript	Het
Acin1	A	G	14: 54,665,204 (GRCm38)	V377A	probably benign	Het
Adam39	A	T	8: 40,825,324 (GRCm38)	I251F	probably damaging	Het
Adnp2	A	T	18: 80,127,697 (GRCm38)	*1166K	probably null	Het
Akap12	T	A	10: 4,357,574 (GRCm38)	D1461E	probably benign	Het
Apba1	A	G	19: 23,893,692 (GRCm38)	D296G	possibly damaging	Het
Apol7a	G	T	15: 77,393,471 (GRCm38)	L26M	possibly damaging	Het
Bcl2	C	T	1: 106,712,392 (GRCm38)	M163I	probably damaging	Het
Cap2	T	G	13: 46,531,013 (GRCm38)	I53R	probably benign	Het
Ccdc105	T	A	10: 78,747,197 (GRCm38)	K451M	probably damaging	Het
Ccdc74a	T	C	16: 17,650,468 (GRCm38)	V318A	possibly damaging	Het
Cd207	C	T	6: 83,675,597 (GRCm38)	V184I	probably benign	Het
Cdk12	C	A	11: 98,241,761 (GRCm38)	C1005*	probably null	Het
Cep83	T	C	10: 94,750,267 (GRCm38)	S344P	probably damaging	Het
Ces1g	A	T	8: 93,306,954 (GRCm38)	Y447N	probably benign	Het
Cfap54	A	T	10: 93,048,061 (GRCm38)	L277Q	probably damaging	Het
Cfh	A	G	1: 140,100,877 (GRCm38)	Y1027H	probably damaging	Het
Clcnkb	T	A	4: 141,415,214 (GRCm38)	I28F	possibly damaging	Het
Clec4d	A	G	6: 123,267,109 (GRCm38)	D59G	probably damaging	Het
Colq	G	A	14: 31,547,452 (GRCm38)	P153S	probably damaging	Het
Crybg1	T	A	10: 43,986,279 (GRCm38)	T1500S	probably damaging	Het
Cyp2d34	T	A	15: 82,617,524 (GRCm38)	R262W	probably damaging	Het
Dennd4b	C	G	3: 90,271,605 (GRCm38)	L559V	probably damaging	Het
Dhrs1	A	G	14: 55,739,309 (GRCm38)	V306A	probably benign	Het
Diaph1	A	T	18: 37,854,573 (GRCm38)	D1043E	possibly damaging	Het
Dis3	G	T	14: 99,086,103 (GRCm38)	D538E	probably damaging	Het
Dnaic2	T	G	11: 114,750,380 (GRCm38)	S344A	probably benign	Het
Dner	C	T	1: 84,445,590 (GRCm38)	V431M	probably damaging	Het
Dnm1l	A	G	16: 16,342,695 (GRCm38)		probably null	Het
Eps15	T	G	4: 109,312,918 (GRCm38)	L139*	probably null	Het
Fam193a	T	A	5: 34,466,292 (GRCm38)	I55N	possibly damaging	Het
Gm10308	T	A	17: 91,088,957 (GRCm38)	Y102*	probably null	Het
Gm10509	A	G	17: 21,690,855 (GRCm38)	K30E	possibly damaging	Het
Gm4778	A	T	3: 94,266,218 (GRCm38)	M174L	probably benign	Het
Gm9573	A	T	17: 35,619,239 (GRCm38)		probably benign	Het
Gpr155	T	C	2: 73,367,577 (GRCm38)	M400V	probably benign	Het
H2-M10.2	T	C	17: 36,286,123 (GRCm38)		probably benign	Het
Heatr1	G	T	13: 12,396,460 (GRCm38)	A61S	probably benign	Het
Helb	G	T	10: 120,094,242 (GRCm38)	T744K	probably damaging	Het
Hmcn1	C	A	1: 150,599,030 (GRCm38)	W4702L	probably damaging	Het
Hmcn2	C	T	2: 31,396,120 (GRCm38)	R2095W	probably damaging	Het
Igfbp3	G	C	11: 7,208,461 (GRCm38)	D267E	probably damaging	Het
Ighmbp2	T	A	19: 3,268,669 (GRCm38)	H469L	probably damaging	Het
Kcnj3	A	C	2: 55,437,220 (GRCm38)	K7T	probably damaging	Het
Krtap5-5	T	G	7: 142,229,621 (GRCm38)	K97N	unknown	Het
Lcor	T	C	19: 41,559,266 (GRCm38)	S430P	probably benign	Het
Lpin1	A	G	12: 16,538,540 (GRCm38)	V883A	probably damaging	Het
Lrp1b	T	C	2: 41,110,825 (GRCm38)	Y2243C	probably damaging	Het
Lrrc46	G	A	11: 97,034,730 (GRCm38)		probably benign	Het
Man1c1	G	C	4: 134,703,438 (GRCm38)	P11R	probably damaging	Het
Mpdz	C	T	4: 81,306,877 (GRCm38)	V1438M	possibly damaging	Het
Ncbp1	T	A	4: 46,169,131 (GRCm38)	L635*	probably null	Het
Nipbl	T	C	15: 8,338,551 (GRCm38)	N1202D	possibly damaging	Het
Nphs1	A	G	7: 30,461,534 (GRCm38)	D196G	probably benign	Het
Nupl1	A	T	14: 60,244,670 (GRCm38)		probably benign	Het
Olfr1008	A	G	2: 85,690,083 (GRCm38)	Y218C	probably damaging	Het
Olfr1360	A	G	13: 21,674,695 (GRCm38)	I83T	probably benign	Het
Olfr901	A	T	9: 38,430,995 (GRCm38)	I238F	probably benign	Het
Pax8	A	T	2: 24,435,821 (GRCm38)	N350K	probably damaging	Het
Pik3cd	T	C	4: 149,658,750 (GRCm38)	K298E	probably benign	Het
Pkd1	C	T	17: 24,594,485 (GRCm38)	R4000C	probably damaging	Het
Pkn2	A	T	3: 142,810,727 (GRCm38)	V546D	possibly damaging	Het
Plcg2	A	G	8: 117,592,708 (GRCm38)	K673E	probably benign	Het
Pld4	T	G	12: 112,763,392 (GRCm38)		probably null	Het
Plek	A	T	11: 16,992,901 (GRCm38)	N130K	probably damaging	Het
Prune2	G	A	19: 17,123,704 (GRCm38)	D2191N	probably benign	Het
Ptgis	T	C	2: 167,206,803 (GRCm38)	Y431C	probably damaging	Het
Rhbdf2	T	C	11: 116,607,266 (GRCm38)	S36G	probably benign	Het
Rtn4ip1	C	T	10: 43,910,830 (GRCm38)	A178V	probably damaging	Het
Rxfp1	A	G	3: 79,670,881 (GRCm38)	S168P	probably benign	Het
Sec24a	A	G	11: 51,733,763 (GRCm38)		probably benign	Het
Shf	G	A	2: 122,368,682 (GRCm38)	P51S	probably damaging	Het
Slitrk6	C	T	14: 110,750,552 (GRCm38)	M574I	probably benign	Het
Slk	T	C	19: 47,622,677 (GRCm38)	F861L	probably damaging	Het
Smpd3	C	A	8: 106,264,971 (GRCm38)	A317S	probably benign	Het
Spz1	T	A	13: 92,575,125 (GRCm38)	Q281L	probably damaging	Het
Syde1	T	A	10: 78,586,980 (GRCm38)	R519S	probably benign	Het
Taf4	T	C	2: 179,976,531 (GRCm38)	H39R	unknown	Het
Tbx5	A	T	5: 119,845,113 (GRCm38)		probably null	Het
Tenm2	C	T	11: 36,063,177 (GRCm38)	G1236R	possibly damaging	Het
Th	G	A	7: 142,898,166 (GRCm38)	Q19*	probably null	Het
Tmprss11a	T	A	5: 86,420,179 (GRCm38)	I230F	probably damaging	Het
Tnfrsf14	T	A	4: 154,925,322 (GRCm38)	H50L	possibly damaging	Het
Tpp2	T	A	1: 43,978,725 (GRCm38)		probably null	Het
Trappc9	G	A	15: 73,025,967 (GRCm38)	R377W	probably damaging	Het
Trim2	A	G	3: 84,190,800 (GRCm38)	I398T	possibly damaging	Het
Trpc5	A	T	X: 144,481,226 (GRCm38)	S212T	probably damaging	Het
Ttn	A	G	2: 76,787,334 (GRCm38)		probably benign	Het
Unc80	A	G	1: 66,639,248 (GRCm38)	T2063A	possibly damaging	Het
Usp37	A	T	1: 74,479,655 (GRCm38)	S260T	probably benign	Het
Vcan	A	G	13: 89,691,681 (GRCm38)	S1915P	probably benign	Het
Vmn1r33	T	A	6: 66,612,298 (GRCm38)	I91F	possibly damaging	Het
Zfp422	T	C	6: 116,626,424 (GRCm38)	T205A	probably benign	Het

Other mutations in Ccnb2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00481:Ccnb2	APN	9	70,418,907 (GRCm38)	missense	probably damaging	0.96
IGL01474:Ccnb2	APN	9	70,419,023 (GRCm38)	missense	probably benign
IGL03097:Ccnb2	APN	9	70,409,396 (GRCm38)	splice site	probably benign
IGL03298:Ccnb2	APN	9	70,418,874 (GRCm38)	missense	probably benign
R0042:Ccnb2	UTSW	9	70,419,053 (GRCm38)	missense	probably benign
R0042:Ccnb2	UTSW	9	70,419,053 (GRCm38)	missense	probably benign
R1585:Ccnb2	UTSW	9	70,410,277 (GRCm38)	splice site	probably null
R2046:Ccnb2	UTSW	9	70,409,347 (GRCm38)	missense	probably benign	0.11
R6045:Ccnb2	UTSW	9	70,419,093 (GRCm38)	missense	probably benign
R7202:Ccnb2	UTSW	9	70,410,846 (GRCm38)	missense	probably damaging	1.00
R7623:Ccnb2	UTSW	9	70,418,888 (GRCm38)	missense	probably benign
R8515:Ccnb2	UTSW	9	70,413,100 (GRCm38)	critical splice donor site	probably null
R9072:Ccnb2	UTSW	9	70,410,813 (GRCm38)	missense	possibly damaging	0.65
R9073:Ccnb2	UTSW	9	70,410,813 (GRCm38)	missense	possibly damaging	0.65
R9235:Ccnb2	UTSW	9	70,410,881 (GRCm38)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GAGCACAGTCAACTTCCCAGAGATG -3'
(R):5'- TGTGATGGCTCTTACAGCCACATTC -3'

Sequencing Primer
(F):5'- TTCCCAGAGATGGTGGGACTC -3'
(R):5'- TACAGCCACATTCAGGTCTC -3'

Posted On

2014-05-23