Incidental Mutation 'R3161:Zfp868'
ID258162
Institutional Source Beutler Lab
Gene Symbol Zfp868
Ensembl Gene ENSMUSG00000060427
Gene Namezinc finger protein 868
Synonyms
MMRRC Submission 040612-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.053) question?
Stock #R3161 (G1)
Quality Score225
Status Validated
Chromosome8
Chromosomal Location69610857-69625548 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 69612085 bp
ZygosityHeterozygous
Amino Acid Change Serine to Proline at position 200 (S200P)
Ref Sequence ENSEMBL: ENSMUSP00000113952 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000074982] [ENSMUST00000121886]
Predicted Effect probably benign
Transcript: ENSMUST00000074982
AA Change: S200P

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)
SMART Domains Protein: ENSMUSP00000074510
Gene: ENSMUSG00000060427
AA Change: S200P

DomainStartEndE-ValueType
KRAB 16 73 1.37e-12 SMART
low complexity region 83 94 N/A INTRINSIC
ZnF_C2H2 155 177 1.06e-4 SMART
ZnF_C2H2 183 205 1.1e-2 SMART
ZnF_C2H2 211 233 7.78e-3 SMART
ZnF_C2H2 239 261 1.36e-2 SMART
ZnF_C2H2 266 288 2.75e-3 SMART
ZnF_C2H2 294 316 4.79e-3 SMART
ZnF_C2H2 322 344 3.89e-3 SMART
ZnF_C2H2 350 372 5.5e-3 SMART
ZnF_C2H2 378 400 3.21e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000121886
AA Change: S200P

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)
SMART Domains Protein: ENSMUSP00000113952
Gene: ENSMUSG00000060427
AA Change: S200P

DomainStartEndE-ValueType
KRAB 16 73 1.37e-12 SMART
low complexity region 83 94 N/A INTRINSIC
ZnF_C2H2 155 177 1.06e-4 SMART
ZnF_C2H2 183 205 1.1e-2 SMART
ZnF_C2H2 211 233 7.78e-3 SMART
ZnF_C2H2 239 261 1.36e-2 SMART
ZnF_C2H2 266 288 2.75e-3 SMART
ZnF_C2H2 294 316 4.79e-3 SMART
ZnF_C2H2 322 344 3.89e-3 SMART
ZnF_C2H2 350 372 5.5e-3 SMART
ZnF_C2H2 378 400 3.21e-4 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000150118
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 97.1%
  • 20x: 94.3%
Validation Efficiency 98% (54/55)
Allele List at MGI
Other mutations in this stock
Total: 52 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
0610040J01Rik T C 5: 63,896,490 probably benign Het
1700066M21Rik T A 1: 57,383,075 N203K probably benign Het
1700125H20Rik A G 11: 85,173,284 S84G probably damaging Het
Adcy9 A G 16: 4,311,588 L715P probably damaging Het
Adgrl2 G A 3: 148,817,551 L1354F probably damaging Het
Amer2 A G 14: 60,378,551 D65G probably damaging Het
Aox2 G T 1: 58,304,438 V427L possibly damaging Het
Atad2b C A 12: 4,939,689 N133K possibly damaging Het
Bptf A T 11: 107,074,476 D1182E probably damaging Het
Camk1g T C 1: 193,359,807 T45A possibly damaging Het
Caps2 C A 10: 112,182,486 Y180* probably null Het
Cfap54 T A 10: 93,045,278 K349N probably damaging Het
Ciz1 A G 2: 32,370,063 D207G probably benign Het
Copa T A 1: 172,091,233 C127S probably damaging Het
Crabp2 A T 3: 87,952,177 K45* probably null Het
Daam1 C A 12: 71,947,098 T425K unknown Het
Dapk2 T G 9: 66,254,611 V267G probably damaging Het
Disp1 T C 1: 183,087,242 K1205E probably benign Het
Dlg4 G C 11: 70,017,225 R4T probably damaging Het
Fbf1 A G 11: 116,148,220 I743T probably damaging Het
Fen1 A G 19: 10,200,291 L263P probably damaging Het
G6pc2 A G 2: 69,220,112 N27S probably damaging Het
Garnl3 A T 2: 33,034,711 N246K probably damaging Het
Gm7337 A C 5: 87,851,557 noncoding transcript Het
Gpr152 A G 19: 4,142,714 T85A probably benign Het
Hnrnpu T C 1: 178,331,125 probably benign Het
Ighv1-81 C G 12: 115,920,329 E101Q probably benign Het
Ipo9 T C 1: 135,409,476 T174A probably benign Het
Myo9a C T 9: 59,832,315 probably benign Het
Nup155 G T 15: 8,148,383 R1083S possibly damaging Het
Olfr1351 C A 10: 79,017,604 T94N probably benign Het
Olfr213 C T 6: 116,540,846 A131V probably damaging Het
Olfr262 T C 19: 12,241,496 H55R probably benign Het
Olfr739 T A 14: 50,425,031 C171S probably damaging Het
Phyh T A 2: 4,937,671 probably benign Het
Pkp4 G T 2: 59,308,105 R233M probably damaging Het
Plcb1 A T 2: 135,335,482 Q578L probably benign Het
Ppil3 A T 1: 58,434,414 N92K probably benign Het
Prokr1 C T 6: 87,588,431 R144H probably damaging Het
Psap T C 10: 60,277,753 L4P possibly damaging Het
Rai14 T C 15: 10,633,164 T47A possibly damaging Het
Rps2 G T 17: 24,720,978 A129S probably benign Het
Sult2a4 T C 7: 13,989,471 T40A probably benign Het
Tacr2 A G 10: 62,265,245 D378G probably benign Het
Topaz1 T C 9: 122,749,381 I452T probably benign Het
Ttn A G 2: 76,833,237 probably benign Het
Vmn2r115 T A 17: 23,357,024 M532K possibly damaging Het
Vmn2r117 A G 17: 23,460,378 L624P probably damaging Het
Vstm5 T G 9: 15,257,298 S53A probably benign Het
Wipf1 C T 2: 73,434,949 E437K probably damaging Het
Wls G T 3: 159,897,436 C162F probably damaging Het
Yeats2 T C 16: 20,193,645 V531A probably damaging Het
Other mutations in Zfp868
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL03166:Zfp868 APN 8 69612314 missense probably damaging 0.99
R0546:Zfp868 UTSW 8 69612231 missense probably benign 0.00
R1706:Zfp868 UTSW 8 69612409 missense probably benign 0.27
R1741:Zfp868 UTSW 8 69611868 missense probably damaging 1.00
R2119:Zfp868 UTSW 8 69611995 nonsense probably null
R2336:Zfp868 UTSW 8 69613907 splice site probably null
R5847:Zfp868 UTSW 8 69611652 missense probably damaging 1.00
R6361:Zfp868 UTSW 8 69611913 missense probably damaging 1.00
R6753:Zfp868 UTSW 8 69612096 missense probably benign 0.08
R6855:Zfp868 UTSW 8 69611579 missense probably damaging 1.00
R8310:Zfp868 UTSW 8 69613795 missense probably damaging 1.00
Z1088:Zfp868 UTSW 8 69611910 frame shift probably null
Z1176:Zfp868 UTSW 8 69612324 missense possibly damaging 0.75
Predicted Primers PCR Primer
(F):5'- ATGAAGGACTTACCACACTCTTT -3'
(R):5'- GAACATCGTCACTCACTCTGAAT -3'

Sequencing Primer
(F):5'- ACAATTATAGAATGTCTCTCCATGC -3'
(R):5'- ACTCACACTGTGAATGGGC -3'
Posted On2015-01-23