Mutagenetix > Incidental Mutations

Incidental Mutation 'IGL02432:Cry2'

293169

Institutional Source

Australian Phenomics Network (link to record)

Gene Symbol

Cry2

Ensembl Gene

ENSMUSG00000068742

Gene Name

cryptochrome 2 (photolyase-like)

Synonyms

Accession Numbers

Is this an essential gene?

Possibly essential (E-score: 0.658) question?

Stock #

IGL02432

Quality Score

Status

Chromosome

Chromosomal Location

92403646-92434043 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 92413667 bp

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 387 (D387G)

Ref Sequence

ENSEMBL: ENSMUSP00000106909 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090559] [ENSMUST00000111278]

Predicted Effect

probably damaging
Transcript: ENSMUST00000090559
AA Change: D387G

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000088047
Gene: ENSMUSG00000068742
AA Change: D387G

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	187	2.4e-50	PFAM
Pfam:FAD_binding_7	231	504	4.4e-89	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000111278
AA Change: D387G

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000106909
Gene: ENSMUSG00000068742
AA Change: D387G

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	189	3.6e-50	PFAM
Pfam:FAD_binding_7	230	506	1.4e-105	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126002

Coding Region Coverage

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour longer circadian period under constant darkness, and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 22 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921509C19Rik	G	A	2: 151,472,561	T399M	probably benign	Het
A830018L16Rik	A	T	1: 11,748,079	N321I	probably damaging	Het
Aimp1	T	C	3: 132,673,977	T135A	probably benign	Het
Ascc3	T	C	10: 50,700,493	M848T	probably damaging	Het
Daxx	G	A	17: 33,912,337	D413N	probably benign	Het
Gbp6	A	T	5: 105,274,362	V492E	probably benign	Het
Itpr2	T	A	6: 146,325,173	M1358L	probably benign	Het
Med13l	T	A	5: 118,738,400	D880E	possibly damaging	Het
Mmd2	A	T	5: 142,575,339	I47N	probably damaging	Het
Mroh2b	G	A	15: 4,914,186	M401I	probably benign	Het
Nrip1	T	C	16: 76,291,780	K963R	probably benign	Het
Otop2	A	G	11: 115,329,162	H276R	probably damaging	Het
Pxn	T	A	5: 115,545,746	D79E	probably damaging	Het
Rho	G	A	6: 115,932,185	V61I	probably damaging	Het
Rtp1	A	G	16: 23,431,404	Y173C	probably damaging	Het
Slc6a17	T	C	3: 107,493,177	D212G	possibly damaging	Het
Stpg1	A	G	4: 135,508,010	E54G	probably damaging	Het
Tas2r119	A	G	15: 32,177,707	T140A	probably benign	Het
Traf3ip3	C	T	1: 193,184,576	C316Y	probably damaging	Het
Ttc28	G	A	5: 111,223,235	A517T	probably damaging	Het
Vmn1r30	T	C	6: 58,435,670	N59S	probably benign	Het
Vmn2r4	C	T	3: 64,406,400	V387M	probably benign	Het

Other mutations in Cry2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01995:Cry2	APN	2	92424632	missense	probably benign	0.15
IGL02167:Cry2	APN	2	92433821	missense	possibly damaging	0.93
IGL02183:Cry2	APN	2	92413039	missense	probably damaging	0.99
IGL02343:Cry2	APN	2	92426921	missense	possibly damaging	0.90
IGL02725:Cry2	APN	2	92413260	splice site	probably benign
IGL02932:Cry2	APN	2	92413117	nonsense	probably null
IGL03122:Cry2	APN	2	92413295	missense	probably damaging	1.00
IGL03366:Cry2	APN	2	92413715	missense	probably damaging	1.00
R0679:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R1325:Cry2	UTSW	2	92413770	missense	probably damaging	1.00
R1862:Cry2	UTSW	2	92424566	missense	probably damaging	1.00
R1891:Cry2	UTSW	2	92413640	missense	possibly damaging	0.93
R2189:Cry2	UTSW	2	92411692	missense	possibly damaging	0.84
R4032:Cry2	UTSW	2	92413827	missense	probably benign	0.00
R4689:Cry2	UTSW	2	92424554	missense	probably benign	0.38
R5130:Cry2	UTSW	2	92424599	missense	probably benign	0.28
R5145:Cry2	UTSW	2	92413060	missense	probably benign
R5970:Cry2	UTSW	2	92412967	missense	probably benign	0.08
R6179:Cry2	UTSW	2	92413842	missense	probably damaging	0.98
R7102:Cry2	UTSW	2	92413093	missense	probably damaging	0.99
R7158:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R7213:Cry2	UTSW	2	92413659	missense	probably benign	0.00
R7257:Cry2	UTSW	2	92412981	missense	possibly damaging	0.67
R7378:Cry2	UTSW	2	92413664	missense	probably damaging	1.00
R7427:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7428:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7440:Cry2	UTSW	2	92413638	missense	probably damaging	1.00
R7531:Cry2	UTSW	2	92413005	missense	probably damaging	0.98
R8234:Cry2	UTSW	2	92412629	missense	probably benign
R8350:Cry2	UTSW	2	92413941	missense	probably benign	0.00
R8450:Cry2	UTSW	2	92413941	missense	probably benign	0.00

Posted On

2015-04-16