Mutagenetix > Incidental Mutation

Incidental Mutation 'R5088:Or10j27'

387549

Institutional Source

Beutler Lab

Gene Symbol

Or10j27

Ensembl Gene

ENSMUSG00000062527

Gene Name

olfactory receptor family 10 subfamily J member 27

Synonyms

MOR267-4, GA_x6K02T2R7CC-715776-716708, Olfr1408

MMRRC Submission

042677-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R5088 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

172957767-172964901 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 172958606 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Isoleucine at position 59 (M59I)

Ref Sequence

ENSEMBL: ENSMUSP00000143864 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073663] [ENSMUST00000200689]

AlphaFold

Q8VG32

Predicted Effect

probably damaging
Transcript: ENSMUST00000073663
AA Change: M59I

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000073345
Gene: ENSMUSG00000062527
AA Change: M59I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1.5e-55	PFAM
Pfam:7tm_1	41	289	9.4e-19	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000200689
AA Change: M59I

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000143864
Gene: ENSMUSG00000062527
AA Change: M59I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1.5e-55	PFAM
Pfam:7tm_1	41	289	9.4e-19	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 93.9%

Validation Efficiency

99% (72/73)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	A	T	5: 109,883,939 (GRCm39)	C640S	probably damaging	Het
4931429L15Rik	T	C	9: 46,217,038 (GRCm39)	R246G	probably benign	Het
Aak1	T	C	6: 86,921,462 (GRCm39)		probably null	Het
Abcc5	A	G	16: 20,195,412 (GRCm39)	L695P	probably damaging	Het
Alcam	T	A	16: 52,109,290 (GRCm39)	T328S	probably damaging	Het
Alpk1	A	G	3: 127,478,969 (GRCm39)		probably benign	Het
Arel1	A	G	12: 84,970,889 (GRCm39)	L642P	probably damaging	Het
Atrip	T	C	9: 108,888,964 (GRCm39)	D741G	probably damaging	Het
Bmp1	T	C	14: 70,723,659 (GRCm39)	T726A	possibly damaging	Het
Bnip3	A	G	7: 138,496,337 (GRCm39)		probably null	Het
Cacng5	C	G	11: 107,768,215 (GRCm39)	C264S	possibly damaging	Het
Calr4	T	A	4: 109,101,859 (GRCm39)		probably benign	Het
Cdhr1	T	A	14: 36,811,422 (GRCm39)	T230S	probably benign	Het
Cdk16	T	C	X: 20,562,566 (GRCm39)		probably benign	Het
Cenpj	T	C	14: 56,791,148 (GRCm39)	E337G	probably damaging	Het
Chd9	A	G	8: 91,704,147 (GRCm39)	I694V	possibly damaging	Het
Chsy3	T	A	18: 59,312,607 (GRCm39)	V360D	probably damaging	Het
Col16a1	C	T	4: 129,972,988 (GRCm39)	T643M	probably benign	Het
Col5a1	G	A	2: 27,908,614 (GRCm39)	W67*	probably null	Het
Cyp20a1	A	T	1: 60,402,509 (GRCm39)	H198L	probably damaging	Het
Dnai1	T	A	4: 41,597,630 (GRCm39)	S38T	probably benign	Het
Dnai1	T	G	4: 41,632,251 (GRCm39)	S562A	probably benign	Het
Dpy19l4	G	T	4: 11,303,357 (GRCm39)	T188K	probably damaging	Het
Ergic2	A	G	6: 148,084,621 (GRCm39)	V304A	probably damaging	Het
Fam83a	A	G	15: 57,873,196 (GRCm39)	T342A	probably benign	Het
Fbxo7	A	G	10: 85,857,784 (GRCm39)		probably benign	Het
Gins4	T	C	8: 23,727,084 (GRCm39)	E3G	possibly damaging	Het
Glis3	G	A	19: 28,508,979 (GRCm39)	P335L	probably benign	Het
Golph3l	A	T	3: 95,524,501 (GRCm39)	T251S	possibly damaging	Het
Hnf4g	C	T	3: 3,722,181 (GRCm39)	A445V	probably benign	Het
Hykk	A	G	9: 54,853,763 (GRCm39)	I362V	probably benign	Het
Il6st	G	A	13: 112,627,089 (GRCm39)	V294M	probably damaging	Het
Itpr1	A	G	6: 108,366,348 (GRCm39)	I92V	probably damaging	Het
Kdm4c	T	C	4: 74,252,936 (GRCm39)	L501P	probably benign	Het
Mpi	G	A	9: 57,457,887 (GRCm39)	T105M	probably damaging	Het
Mrpl37	A	T	4: 106,921,919 (GRCm39)	L132H	probably damaging	Het
Muc5ac	A	T	7: 141,350,056 (GRCm39)	T600S	possibly damaging	Het
Npl	A	T	1: 153,384,890 (GRCm39)	I249N	possibly damaging	Het
Or7g30	A	G	9: 19,353,067 (GRCm39)	N286S	probably damaging	Het
Pkd1	A	G	17: 24,809,812 (GRCm39)	K3497E	possibly damaging	Het
Ptprt	T	C	2: 162,080,095 (GRCm39)	N235D	probably benign	Het
Rangap1	A	C	15: 81,594,664 (GRCm39)	D388E	probably benign	Het
Rcvrn	A	T	11: 67,586,340 (GRCm39)	Q33L	probably benign	Het
Rif1	A	T	2: 51,982,307 (GRCm39)	K524I	possibly damaging	Het
Rnf139	A	T	15: 58,771,790 (GRCm39)	N605I	possibly damaging	Het
Ryr2	C	A	13: 11,727,129 (GRCm39)	G2411*	probably null	Het
Scn3a	A	T	2: 65,302,643 (GRCm39)	S1268T	probably damaging	Het
Sema6a	T	A	18: 47,382,196 (GRCm39)	I784F	probably damaging	Het
Sgms2	C	T	3: 131,124,005 (GRCm39)	V232M	probably benign	Het
Skint7	T	C	4: 111,837,627 (GRCm39)	I135T	possibly damaging	Het
Smchd1	A	T	17: 71,738,343 (GRCm39)	N507K	possibly damaging	Het
Spata31d1a	A	C	13: 59,848,966 (GRCm39)		probably null	Het
Spata7	A	T	12: 98,635,761 (GRCm39)	D527V	probably benign	Het
Srcap	A	G	7: 127,140,833 (GRCm39)	T1477A	probably benign	Het
Srl	C	A	16: 4,300,633 (GRCm39)	D375Y	probably damaging	Het
Stk4	A	T	2: 163,925,608 (GRCm39)	K58*	probably null	Het
Svep1	T	G	4: 58,120,648 (GRCm39)	H795P	possibly damaging	Het
Trak2	T	A	1: 58,974,967 (GRCm39)	Q75L	probably benign	Het
Ubxn2a	T	A	12: 4,933,904 (GRCm39)	N169I	probably damaging	Het
Vmn1r216	T	A	13: 23,283,473 (GRCm39)	L52*	probably null	Het
Vmn2r108	A	T	17: 20,690,454 (GRCm39)	N459K	possibly damaging	Het
Vstm5	A	G	9: 15,168,601 (GRCm39)	D55G	possibly damaging	Het
Wdr24	T	A	17: 26,047,181 (GRCm39)		probably null	Het
Zfp13	C	A	17: 23,796,035 (GRCm39)	E179*	probably null	Het
Zfp839	T	C	12: 110,834,610 (GRCm39)	S546P	probably damaging	Het

Other mutations in Or10j27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00323:Or10j27	APN	1	172,957,978 (GRCm39)	nonsense	probably null
IGL01371:Or10j27	APN	1	172,958,098 (GRCm39)	missense	possibly damaging	0.67
IGL01816:Or10j27	APN	1	172,957,840 (GRCm39)	utr 3 prime	probably benign
IGL02543:Or10j27	APN	1	172,957,901 (GRCm39)	missense	probably damaging	0.99
IGL03104:Or10j27	APN	1	172,958,526 (GRCm39)	missense	probably benign	0.00
IGL03183:Or10j27	APN	1	172,958,425 (GRCm39)	missense	probably damaging	0.97
R0940:Or10j27	UTSW	1	172,958,020 (GRCm39)	missense	probably benign	0.42
R1835:Or10j27	UTSW	1	172,958,382 (GRCm39)	missense	probably benign	0.00
R1993:Or10j27	UTSW	1	172,958,418 (GRCm39)	missense	possibly damaging	0.65
R1994:Or10j27	UTSW	1	172,958,418 (GRCm39)	missense	possibly damaging	0.65
R4438:Or10j27	UTSW	1	172,957,869 (GRCm39)	missense	probably benign	0.02
R4948:Or10j27	UTSW	1	172,958,526 (GRCm39)	missense	probably benign	0.00
R5117:Or10j27	UTSW	1	172,958,484 (GRCm39)	missense	possibly damaging	0.91
R5118:Or10j27	UTSW	1	172,958,484 (GRCm39)	missense	possibly damaging	0.91
R5344:Or10j27	UTSW	1	172,958,673 (GRCm39)	missense	probably benign	0.16
R8176:Or10j27	UTSW	1	172,958,383 (GRCm39)	missense	probably benign	0.00
X0018:Or10j27	UTSW	1	172,958,266 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- TGGCCACATAGCGATCATAACC -3'
(R):5'- TAGATTTCAGATCGAAGGCCCC -3'

Sequencing Primer
(F):5'- GCGATCATAACCCATGACTGCTAG -3'
(R):5'- TTCAGATCGAAGGCCCCCAATG -3'

Posted On

2016-06-06