|Institutional Source||Beutler Lab|
|Gene Name||Kruppel-like factor 5|
|Synonyms||IKLF, Bteb2, 4930520J07Rik, CKLF|
|Is this an essential gene?||Essential (E-score: 1.000)|
|Stock #||R5437 (G1)|
|Chromosomal Location||99298691-99315036 bp(+) (GRCm38)|
|Type of Mutation||nonsense|
|DNA Base Change (assembly)||C to T at 99301459 bp (GRCm38)|
|Amino Acid Change||Arginine to Stop codon at position 23 (R23*)|
|Ref Sequence||ENSEMBL: ENSMUSP00000154786 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000005279] [ENSMUST00000226784]|
AA Change: R103*
AA Change: R103*
AA Change: R23*
|Meta Mutation Damage Score||0.9755|
|Coding Region Coverage||
|Validation Efficiency||97% (63/65)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
PHENOTYPE: Homozygous null mice die during gestation, while heterozygotes exhibit abnormal cardiovascular remodeling after external stress. Mice homozygous for a floxed allele activated in the prostate exhibit increased cell proliferation and hyperplasia in the prostate without neoplasia. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Klf5||
(F):5'- GTCGTAGAGTCTTGGTTAACCG -3'
(R):5'- CTGGTGGCTGAAAATGGTAAC -3'
(F):5'- TAAGCAACACTTAGGAGTTGGCCC -3'
(R):5'- AACAGGTTCTGTCTTGATCTGTG -3'