Mutagenetix > Incidental Mutations

Incidental Mutation 'R5649:Wdr86'

441313

Institutional Source

Beutler Lab

Gene Symbol

Wdr86

Ensembl Gene

ENSMUSG00000055235

Gene Name

WD repeat domain 86

Synonyms

MMRRC Submission

043170-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.085) question?

Stock #

R5649 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

24711738-24730727 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 24718087 bp

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 202 (H202Q)

Ref Sequence

ENSEMBL: ENSMUSP00000064785 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068693]

Predicted Effect

probably benign
Transcript: ENSMUST00000068693
AA Change: H202Q

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000064785
Gene: ENSMUSG00000055235
AA Change: H202Q

Domain	Start	End	E-Value	Type
WD40	4	43	1.62e-8	SMART
WD40	46	83	3.17e-2	SMART
WD40	86	123	1.9e-5	SMART
WD40	126	179	3e-3	SMART
WD40	182	221	2.78e-7	SMART
WD40	224	261	9.9e-4	SMART
WD40	264	301	1.29e-2	SMART
WD40	304	341	6.28e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000127320

SMART Domains

Protein: ENSMUSP00000119649
Gene: ENSMUSG00000055235

Domain	Start	End	E-Value	Type
WD40	29	63	1.27e2	SMART
WD40	66	103	6.28e-6	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142607

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196443

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.5%
20x: 96.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610009B22Rik	T	C	11: 51,685,972	E33G	probably benign	Het
Abca12	T	C	1: 71,291,342	T1385A	probably damaging	Het
Apc2	T	A	10: 80,314,138	D1646E	probably damaging	Het
Aspm	G	A	1: 139,479,669	R2098H	probably benign	Het
Atl3	C	A	19: 7,532,227	T435N	possibly damaging	Het
Cdh22	G	T	2: 165,116,280	T589K	probably damaging	Het
Cnot3	T	C	7: 3,658,083	L561S	probably benign	Het
Col5a1	A	G	2: 27,951,456	D363G	unknown	Het
Cyp24a1	T	C	2: 170,496,309	D105G	possibly damaging	Het
Dennd4a	C	A	9: 64,851,209		probably null	Het
Dnah8	A	G	17: 30,800,587	K3878R	probably benign	Het
Dock4	T	C	12: 40,844,540	S1900P	probably benign	Het
Fancg	A	G	4: 43,008,736	L167P	probably damaging	Het
Ighd2-8	A	G	12: 113,450,867	S1P	possibly damaging	Het
Kif28	A	G	1: 179,697,771		probably null	Het
Mrpl55	T	A	11: 59,204,571	C20*	probably null	Het
Myo5a	A	G	9: 75,171,719	K920E	possibly damaging	Het
Naa35	G	A	13: 59,622,866		probably benign	Het
Olfm3	A	G	3: 115,096,924	R76G	probably damaging	Het
Olfr304	T	C	7: 86,386,313	M116V	probably damaging	Het
Olfr705	T	C	7: 106,714,166	R172G	possibly damaging	Het
Pcdha12	A	T	18: 37,022,415	D729V	probably benign	Het
Phf11c	T	C	14: 59,385,532		probably null	Het
Phf20	T	A	2: 156,251,768		probably null	Het
Plbd1	T	A	6: 136,616,989	Y376F	probably benign	Het
Poglut1	A	G	16: 38,531,811	V257A	probably damaging	Het
Reln	A	G	5: 21,901,625	I3249T	probably benign	Het
Rgsl1	G	A	1: 153,825,893	P272S	possibly damaging	Het
Slc15a2	A	T	16: 36,772,110	Y197*	probably null	Het
Slc45a2	C	T	15: 11,012,607	T232I	probably benign	Het
Ssc5d	T	A	7: 4,926,518		probably null	Het
Thbs2	T	C	17: 14,689,953	Y128C	probably damaging	Het
Them4	A	T	3: 94,331,544	L219F	possibly damaging	Het
Tmem30b	G	T	12: 73,546,166	N58K	probably benign	Het
Ttc29	G	A	8: 78,246,313	E131K	possibly damaging	Het
Vmn1r29	C	G	6: 58,307,691	S132C	probably benign	Het
Vmn1r53	G	A	6: 90,223,760	A194V	probably benign	Het
Xirp2	A	G	2: 67,516,895	D3160G	probably benign	Het
Xkr5	T	C	8: 18,933,966	D520G	probably benign	Het
Zfp607b	T	G	7: 27,703,981	C621G	probably damaging	Het

Other mutations in Wdr86

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02419:Wdr86	APN	5	24722704	missense	probably damaging	1.00
R0412:Wdr86	UTSW	5	24718234	missense	probably benign	0.01
R0899:Wdr86	UTSW	5	24718007	missense	probably benign	0.15
R2520:Wdr86	UTSW	5	24712575	makesense	probably null
R3522:Wdr86	UTSW	5	24718307	missense	probably benign	0.00
R3686:Wdr86	UTSW	5	24718341	missense	probably damaging	1.00
R4439:Wdr86	UTSW	5	24730237	missense	probably damaging	1.00
R4657:Wdr86	UTSW	5	24718231	missense	probably benign	0.00
R4989:Wdr86	UTSW	5	24712845	splice site	probably null
R5940:Wdr86	UTSW	5	24722662	missense	probably damaging	1.00
R6254:Wdr86	UTSW	5	24718283	missense	probably benign	0.04
R8012:Wdr86	UTSW	5	24730179	critical splice donor site	probably null
R8395:Wdr86	UTSW	5	24730189	missense	probably damaging	1.00
Z1177:Wdr86	UTSW	5	24712748	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAATGGCCTTGAGTTCCCTC -3'
(R):5'- CAAGTGTCCCAAGAGTTCCG -3'

Sequencing Primer
(F):5'- AATGGCCTTGAGTTCCCTCCATTC -3'
(R):5'- GCCACCGTAACTGTGTACTGAC -3'

Posted On

2016-11-08