Incidental Mutation 'R7858:Zfp961'
| ID |
607336 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp961
|
| Ensembl Gene |
ENSMUSG00000052446 |
| Gene Name |
zinc finger protein 961 |
| Synonyms |
A230105L22Rik, BC049349 |
| MMRRC Submission |
045911-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R7858 (G1)
|
| Quality Score |
165.009 |
|
Status
|
Validated
|
| Chromosome |
8 |
| Chromosomal Location |
72704910-72724177 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 72704949 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Valine to Alanine
at position 4
(V4A)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000105623
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000109997]
[ENSMUST00000131237]
[ENSMUST00000132848]
[ENSMUST00000136516]
[ENSMUST00000138304]
[ENSMUST00000145256]
|
| AlphaFold |
E9Q4R5 |
| Predicted Effect |
unknown
Transcript: ENSMUST00000109997
AA Change: V4A
|
| SMART Domains |
Protein: ENSMUSP00000105623
Gene: ENSMUSG00000052446
AA Change: V4A
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
25 |
82 |
1.14e-18 |
SMART |
|
ZnF_C2H2
|
144 |
166 |
1.69e-3 |
SMART |
|
ZnF_C2H2
|
172 |
194 |
3.07e-1 |
SMART |
|
ZnF_C2H2
|
199 |
221 |
1.36e-2 |
SMART |
|
ZnF_C2H2
|
226 |
248 |
3.29e-1 |
SMART |
|
ZnF_C2H2
|
254 |
276 |
3.34e-2 |
SMART |
|
ZnF_C2H2
|
282 |
304 |
9.58e-3 |
SMART |
|
ZnF_C2H2
|
310 |
332 |
2.17e-1 |
SMART |
|
ZnF_C2H2
|
338 |
360 |
1.04e-3 |
SMART |
|
ZnF_C2H2
|
366 |
388 |
2.24e-3 |
SMART |
|
ZnF_C2H2
|
394 |
416 |
2.24e-3 |
SMART |
|
ZnF_C2H2
|
421 |
443 |
4.61e-5 |
SMART |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000131237
|
| SMART Domains |
Protein: ENSMUSP00000122942
Gene: ENSMUSG00000052446
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
6 |
63 |
1.14e-18 |
SMART |
|
ZnF_C2H2
|
125 |
147 |
1.69e-3 |
SMART |
|
ZnF_C2H2
|
153 |
175 |
3.07e-1 |
SMART |
|
ZnF_C2H2
|
180 |
202 |
1.36e-2 |
SMART |
|
ZnF_C2H2
|
207 |
229 |
3.29e-1 |
SMART |
|
ZnF_C2H2
|
235 |
257 |
3.34e-2 |
SMART |
|
ZnF_C2H2
|
263 |
285 |
9.58e-3 |
SMART |
|
ZnF_C2H2
|
291 |
313 |
2.17e-1 |
SMART |
|
ZnF_C2H2
|
319 |
341 |
1.04e-3 |
SMART |
|
ZnF_C2H2
|
347 |
369 |
2.24e-3 |
SMART |
|
ZnF_C2H2
|
375 |
397 |
2.24e-3 |
SMART |
|
ZnF_C2H2
|
402 |
424 |
4.61e-5 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000132848
AA Change: V4A
PolyPhen 2
Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)
|
| SMART Domains |
Protein: ENSMUSP00000114730
Gene: ENSMUSG00000052446
AA Change: V4A
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
25 |
82 |
1.14e-18 |
SMART |
|
ZnF_C2H2
|
144 |
166 |
1.69e-3 |
SMART |
|
| Predicted Effect |
|
| SMART Domains |
Protein: ENSMUSP00000122589
Gene: ENSMUSG00000052446
AA Change: V4A
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
25 |
82 |
1.14e-18 |
SMART |
|
ZnF_C2H2
|
144 |
166 |
1.69e-3 |
SMART |
|
ZnF_C2H2
|
172 |
194 |
3.07e-1 |
SMART |
|
ZnF_C2H2
|
199 |
221 |
1.36e-2 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000138304
AA Change: V4A
PolyPhen 2
Score 0.032 (Sensitivity: 0.95; Specificity: 0.82)
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000145256
AA Change: V4A
PolyPhen 2
Score 0.032 (Sensitivity: 0.95; Specificity: 0.82)
|
| Meta Mutation Damage Score |
0.0852 |
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 100.0%
- 10x: 99.8%
- 20x: 99.2%
|
| Validation Efficiency |
100% (32/32) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 31 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Acadl |
T |
C |
1: 66,877,483 (GRCm39) |
E356G |
probably benign |
Het |
| Adamtsl3 |
T |
C |
7: 82,099,371 (GRCm39) |
L175P |
probably damaging |
Het |
| Apon |
T |
C |
10: 128,090,328 (GRCm39) |
I2T |
probably benign |
Het |
| Ccny |
T |
A |
18: 9,386,782 (GRCm39) |
D61V |
probably damaging |
Het |
| Cp |
A |
G |
3: 20,025,219 (GRCm39) |
T393A |
probably benign |
Het |
| Cspg5 |
T |
C |
9: 110,080,134 (GRCm39) |
L434P |
probably damaging |
Het |
| Cyld |
T |
A |
8: 89,436,616 (GRCm39) |
I302N |
probably damaging |
Het |
| Ercc4 |
T |
C |
16: 12,943,169 (GRCm39) |
S283P |
probably damaging |
Het |
| Frem3 |
T |
A |
8: 81,338,350 (GRCm39) |
Y214* |
probably null |
Het |
| Gm1123 |
T |
C |
9: 98,896,107 (GRCm39) |
N258D |
possibly damaging |
Het |
| Gm9821 |
A |
T |
2: 91,776,351 (GRCm39) |
H101L |
unknown |
Het |
| Il36b |
T |
A |
2: 24,044,626 (GRCm39) |
C9S |
probably benign |
Het |
| Kti12 |
A |
C |
4: 108,705,443 (GRCm39) |
E119A |
probably benign |
Het |
| Kti12 |
G |
T |
4: 108,705,444 (GRCm39) |
E119D |
probably benign |
Het |
| Mansc1 |
T |
C |
6: 134,587,377 (GRCm39) |
T267A |
probably benign |
Het |
| Mtor |
A |
G |
4: 148,539,103 (GRCm39) |
D200G |
probably damaging |
Het |
| Muc5ac |
G |
C |
7: 141,357,166 (GRCm39) |
V1148L |
possibly damaging |
Het |
| Mycbp2 |
C |
A |
14: 103,393,741 (GRCm39) |
R2940L |
probably damaging |
Het |
| Myh7 |
A |
G |
14: 55,227,500 (GRCm39) |
F312L |
probably benign |
Het |
| Or4c31 |
G |
A |
2: 88,292,056 (GRCm39) |
C143Y |
probably damaging |
Het |
| Prkdc |
T |
A |
16: 15,507,141 (GRCm39) |
S874T |
probably benign |
Het |
| Prmt7 |
T |
G |
8: 106,971,320 (GRCm39) |
I452S |
possibly damaging |
Het |
| Setx |
A |
C |
2: 29,051,562 (GRCm39) |
D2038A |
probably damaging |
Het |
| Spen |
A |
T |
4: 141,215,442 (GRCm39) |
|
probably null |
Het |
| Sycp1 |
T |
C |
3: 102,806,273 (GRCm39) |
K473E |
probably benign |
Het |
| Uggt1 |
A |
G |
1: 36,195,339 (GRCm39) |
F1290S |
probably damaging |
Het |
| Unc13b |
T |
G |
4: 43,176,285 (GRCm39) |
V2371G |
unknown |
Het |
| Vmn2r4 |
C |
T |
3: 64,317,226 (GRCm39) |
V171I |
probably benign |
Het |
| Zfp35 |
T |
C |
18: 24,136,897 (GRCm39) |
C414R |
probably damaging |
Het |
| Zfp704 |
A |
G |
3: 9,509,217 (GRCm39) |
|
probably null |
Het |
| Zfp758 |
A |
G |
17: 22,594,359 (GRCm39) |
T282A |
probably benign |
Het |
|
| Other mutations in Zfp961 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00595:Zfp961
|
APN |
8 |
72,722,272 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00801:Zfp961
|
APN |
8 |
72,719,732 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00825:Zfp961
|
APN |
8 |
72,721,888 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
IGL01405:Zfp961
|
APN |
8 |
72,721,778 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
IGL03100:Zfp961
|
APN |
8 |
72,721,754 (GRCm39) |
makesense |
probably null |
|
|
R0969:Zfp961
|
UTSW |
8 |
72,722,139 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4851:Zfp961
|
UTSW |
8 |
72,722,847 (GRCm39) |
unclassified |
probably benign |
|
|
R5502:Zfp961
|
UTSW |
8 |
72,721,903 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5813:Zfp961
|
UTSW |
8 |
72,722,070 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6762:Zfp961
|
UTSW |
8 |
72,719,958 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R6902:Zfp961
|
UTSW |
8 |
72,722,522 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8749:Zfp961
|
UTSW |
8 |
72,719,686 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9718:Zfp961
|
UTSW |
8 |
72,721,933 (GRCm39) |
missense |
possibly damaging |
0.81 |
|
X0027:Zfp961
|
UTSW |
8 |
72,721,793 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
| Predicted Primers |
PCR Primer
(F):5'- TTCCGGACTATGTTGAAGGCG -3'
(R):5'- GCAAATTAACTCCGTGGGGAC -3'
Sequencing Primer
(F):5'- ACTATGTTGAAGGCGATAGGTC -3'
(R):5'- CTGCGGGCTCTGGAAAAG -3'
|
| Posted On |
2019-12-20 |
Incidental Mutation