Mutagenetix > Incidental Mutation

Incidental Mutation 'R8232:Vmn2r58'

637176

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r58

Ensembl Gene

ENSMUSG00000090383

Gene Name

vomeronasal 2, receptor 58

Synonyms

EG628422

MMRRC Submission

067664-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.377) question?

Stock #

R8232 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

41486305-41522094 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 41514076 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 189 (M189K)

Ref Sequence

ENSEMBL: ENSMUSP00000126966 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171671]

AlphaFold

K7N6V2

Predicted Effect

probably damaging
Transcript: ENSMUST00000171671
AA Change: M189K

PolyPhen 2 Score 0.967 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000126966
Gene: ENSMUSG00000090383
AA Change: M189K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	8e-43	PFAM
Pfam:NCD3G	514	567	1.8e-23	PFAM
Pfam:7tm_3	597	835	2.9e-52	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.4%
20x: 98.5%

Validation Efficiency

100% (34/34)

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acnat1	A	T	4: 49,450,817 (GRCm39)	M98K	probably damaging	Het
Actn3	A	T	19: 4,921,683 (GRCm39)	W166R	probably damaging	Het
Adamts16	C	A	13: 70,941,217 (GRCm39)	C390F	probably damaging	Het
Arhgap32	A	T	9: 32,168,198 (GRCm39)	D727V	probably damaging	Het
Bcr	A	G	10: 75,001,883 (GRCm39)	Y929C	probably damaging	Het
Bmp1	T	A	14: 70,757,329 (GRCm39)	Y50F	probably damaging	Het
Camta2	G	A	11: 70,573,841 (GRCm39)	R171C	unknown	Het
Ccnd2	A	G	6: 127,127,549 (GRCm39)	L63P	probably damaging	Het
Clip1	G	C	5: 123,785,981 (GRCm39)	D234E	probably benign	Het
Dnah9	A	T	11: 65,746,149 (GRCm39)	I3988N	possibly damaging	Het
Frmpd2	T	G	14: 33,261,724 (GRCm39)	I843S	probably damaging	Het
Gcgr	A	G	11: 120,427,328 (GRCm39)	Y146C	probably damaging	Het
Gm5930	T	G	14: 44,573,181 (GRCm39)	K158T	probably damaging	Het
Gnl1	T	A	17: 36,298,487 (GRCm39)	C410*	probably null	Het
Gtf2h1	T	A	7: 46,451,103 (GRCm39)	I4K	probably benign	Het
Il12b	A	G	11: 44,299,401 (GRCm39)	S157G	possibly damaging	Het
Jcad	G	T	18: 4,674,862 (GRCm39)	A875S	probably benign	Het
Kcnrg	T	A	14: 61,845,386 (GRCm39)	M142K	probably benign	Het
Krtap4-6	A	C	11: 99,556,568 (GRCm39)	L53R	unknown	Het
Myo10	G	A	15: 25,804,400 (GRCm39)	G1565D	possibly damaging	Het
Nf1	A	G	11: 79,469,157 (GRCm39)	S806G	probably damaging	Het
Nlrp5	G	T	7: 23,116,770 (GRCm39)	V165L	probably benign	Het
Nppa	T	C	4: 148,085,795 (GRCm39)	I134T	possibly damaging	Het
Or4a72	A	T	2: 89,405,938 (GRCm39)	V44E	noncoding transcript	Het
Or51ai2	G	A	7: 103,586,980 (GRCm39)	R131Q	possibly damaging	Het
Or5b107	A	G	19: 13,142,683 (GRCm39)	M102V	probably benign	Het
Or5p68	A	T	7: 107,945,495 (GRCm39)	M231K	probably damaging	Het
Or6c202	C	A	10: 128,996,097 (GRCm39)	C252F	probably damaging	Het
Scyl2	A	T	10: 89,498,309 (GRCm39)	F167I	probably damaging	Het
Smarcal1	T	C	1: 72,665,722 (GRCm39)	L740S	probably damaging	Het
Tmem183a	T	A	1: 134,277,918 (GRCm39)	D282V	probably damaging	Het
Trhde	A	T	10: 114,636,442 (GRCm39)	V255E	possibly damaging	Het
Vmn1r229	T	A	17: 21,035,309 (GRCm39)	C185S	probably damaging	Het
Vps50	T	G	6: 3,600,139 (GRCm39)	V866G	probably damaging	Het

Other mutations in Vmn2r58

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Vmn2r58	APN	7	41,513,854 (GRCm39)	missense	possibly damaging	0.53
IGL00924:Vmn2r58	APN	7	41,486,891 (GRCm39)	missense	probably damaging	1.00
IGL01291:Vmn2r58	APN	7	41,513,935 (GRCm39)	missense	probably benign	0.02
IGL01480:Vmn2r58	APN	7	41,514,116 (GRCm39)	missense	probably benign	0.01
IGL01551:Vmn2r58	APN	7	41,514,703 (GRCm39)	missense	probably damaging	0.97
IGL01591:Vmn2r58	APN	7	41,514,753 (GRCm39)	missense	probably benign	0.03
IGL01940:Vmn2r58	APN	7	41,487,071 (GRCm39)	missense	probably benign	0.00
IGL01994:Vmn2r58	APN	7	41,486,394 (GRCm39)	missense	probably damaging	0.98
IGL02041:Vmn2r58	APN	7	41,514,703 (GRCm39)	missense	probably damaging	0.97
IGL02222:Vmn2r58	APN	7	41,513,449 (GRCm39)	missense	possibly damaging	0.81
IGL02317:Vmn2r58	APN	7	41,486,765 (GRCm39)	missense	possibly damaging	0.89
IGL02614:Vmn2r58	APN	7	41,486,553 (GRCm39)	missense	probably damaging	1.00
IGL02673:Vmn2r58	APN	7	41,514,082 (GRCm39)	missense	possibly damaging	0.90
IGL03323:Vmn2r58	APN	7	41,511,295 (GRCm39)	missense	probably benign	0.06
IGL03337:Vmn2r58	APN	7	41,513,810 (GRCm39)	missense	possibly damaging	0.93
IGL03380:Vmn2r58	APN	7	41,513,874 (GRCm39)	missense	probably benign	0.00
ANU05:Vmn2r58	UTSW	7	41,513,935 (GRCm39)	missense	probably benign	0.02
R0138:Vmn2r58	UTSW	7	41,487,048 (GRCm39)	missense	probably damaging	1.00
R0141:Vmn2r58	UTSW	7	41,511,309 (GRCm39)	missense	probably benign	0.11
R0421:Vmn2r58	UTSW	7	41,514,628 (GRCm39)	missense	probably benign	0.02
R0604:Vmn2r58	UTSW	7	41,510,000 (GRCm39)	missense	possibly damaging	0.78
R0854:Vmn2r58	UTSW	7	41,486,562 (GRCm39)	missense	probably damaging	1.00
R1413:Vmn2r58	UTSW	7	41,513,387 (GRCm39)	missense	probably benign	0.01
R1441:Vmn2r58	UTSW	7	41,486,864 (GRCm39)	missense	probably damaging	1.00
R1678:Vmn2r58	UTSW	7	41,513,480 (GRCm39)	missense	probably benign	0.40
R1691:Vmn2r58	UTSW	7	41,486,913 (GRCm39)	missense	possibly damaging	0.95
R1699:Vmn2r58	UTSW	7	41,509,951 (GRCm39)	missense	probably benign
R1865:Vmn2r58	UTSW	7	41,486,682 (GRCm39)	missense	possibly damaging	0.95
R2008:Vmn2r58	UTSW	7	41,509,924 (GRCm39)	missense	probably damaging	1.00
R2036:Vmn2r58	UTSW	7	41,513,417 (GRCm39)	missense	probably benign
R2202:Vmn2r58	UTSW	7	41,513,594 (GRCm39)	missense	probably benign	0.07
R3787:Vmn2r58	UTSW	7	41,513,498 (GRCm39)	missense	probably benign	0.01
R3883:Vmn2r58	UTSW	7	41,513,914 (GRCm39)	nonsense	probably null
R3944:Vmn2r58	UTSW	7	41,513,885 (GRCm39)	missense	probably benign	0.03
R3949:Vmn2r58	UTSW	7	41,513,348 (GRCm39)	missense	probably benign	0.08
R4232:Vmn2r58	UTSW	7	41,487,011 (GRCm39)	missense	possibly damaging	0.91
R4409:Vmn2r58	UTSW	7	41,522,051 (GRCm39)	missense	possibly damaging	0.69
R4411:Vmn2r58	UTSW	7	41,511,360 (GRCm39)	missense	possibly damaging	0.85
R4413:Vmn2r58	UTSW	7	41,511,360 (GRCm39)	missense	possibly damaging	0.85
R4600:Vmn2r58	UTSW	7	41,522,046 (GRCm39)	missense	probably benign	0.03
R4610:Vmn2r58	UTSW	7	41,487,117 (GRCm39)	missense	probably benign
R4646:Vmn2r58	UTSW	7	41,509,935 (GRCm39)	missense	probably damaging	0.96
R4793:Vmn2r58	UTSW	7	41,514,495 (GRCm39)	missense	probably damaging	0.99
R4870:Vmn2r58	UTSW	7	41,486,639 (GRCm39)	missense	possibly damaging	0.76
R4981:Vmn2r58	UTSW	7	41,486,885 (GRCm39)	missense	probably damaging	1.00
R4993:Vmn2r58	UTSW	7	41,487,176 (GRCm39)	missense	probably benign	0.00
R5024:Vmn2r58	UTSW	7	41,513,746 (GRCm39)	missense	probably damaging	0.99
R5064:Vmn2r58	UTSW	7	41,486,534 (GRCm39)	missense	probably damaging	0.99
R5330:Vmn2r58	UTSW	7	41,513,384 (GRCm39)	nonsense	probably null
R5526:Vmn2r58	UTSW	7	41,522,069 (GRCm39)	missense	probably benign	0.01
R5980:Vmn2r58	UTSW	7	41,514,480 (GRCm39)	missense	possibly damaging	0.81
R6163:Vmn2r58	UTSW	7	41,486,825 (GRCm39)	missense	probably benign	0.31
R6365:Vmn2r58	UTSW	7	41,513,607 (GRCm39)	missense	probably benign	0.42
R6567:Vmn2r58	UTSW	7	41,514,673 (GRCm39)	missense	probably benign	0.34
R6594:Vmn2r58	UTSW	7	41,486,535 (GRCm39)	missense	possibly damaging	0.69
R6980:Vmn2r58	UTSW	7	41,513,662 (GRCm39)	missense	possibly damaging	0.64
R7373:Vmn2r58	UTSW	7	41,487,212 (GRCm39)	missense	probably damaging	1.00
R7458:Vmn2r58	UTSW	7	41,487,123 (GRCm39)	missense	probably benign	0.06
R7630:Vmn2r58	UTSW	7	41,513,611 (GRCm39)	missense	probably damaging	0.99
R7807:Vmn2r58	UTSW	7	41,521,910 (GRCm39)	missense	probably benign	0.05
R8114:Vmn2r58	UTSW	7	41,511,392 (GRCm39)	missense	probably damaging	1.00
R8313:Vmn2r58	UTSW	7	41,521,952 (GRCm39)	missense	probably benign	0.01
R8412:Vmn2r58	UTSW	7	41,513,722 (GRCm39)	missense	probably benign	0.01
R8530:Vmn2r58	UTSW	7	41,513,576 (GRCm39)	missense	probably damaging	1.00
R8851:Vmn2r58	UTSW	7	41,487,219 (GRCm39)	missense	probably benign
R8881:Vmn2r58	UTSW	7	41,486,609 (GRCm39)	missense	probably benign	0.05
R8936:Vmn2r58	UTSW	7	41,513,981 (GRCm39)	missense
R9045:Vmn2r58	UTSW	7	41,487,087 (GRCm39)	missense	probably benign	0.00
R9166:Vmn2r58	UTSW	7	41,513,431 (GRCm39)	missense	probably damaging	1.00
R9706:Vmn2r58	UTSW	7	41,510,000 (GRCm39)	missense	probably damaging	0.99
RF006:Vmn2r58	UTSW	7	41,486,383 (GRCm39)	frame shift	probably null
RF027:Vmn2r58	UTSW	7	41,486,383 (GRCm39)	frame shift	probably null
Z1176:Vmn2r58	UTSW	7	41,513,789 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GACATTTGCTGATGATTCCTCAATC -3'
(R):5'- TTGCAATTGGAGATCTGTCAGC -3'

Sequencing Primer
(F):5'- CCATAGTTCATTGGAAAATGCATTC -3'
(R):5'- CAGGACAAGTCCATCTTCAT -3'

Posted On

2020-07-13