Mutagenetix > Incidental Mutation

Incidental Mutation 'R7920:Zfp260'

648290

Institutional Source

Beutler Lab

Gene Symbol

Zfp260

Ensembl Gene

ENSMUSG00000049421

Gene Name

zinc finger protein 260

Synonyms

Zfp63, PEX1, Ozrf1

MMRRC Submission

045967-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.122) question?

Stock #

R7920 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

30094777-30107622 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 30105592 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 306 (K306*)

Ref Sequence

ENSEMBL: ENSMUSP00000052200 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000050735] [ENSMUST00000108200] [ENSMUST00000130526]

AlphaFold

Q62513

Predicted Effect

probably null
Transcript: ENSMUST00000050735
AA Change: K306*

SMART Domains

Protein: ENSMUSP00000052200
Gene: ENSMUSG00000049421
AA Change: K306*

Domain	Start	End	E-Value	Type
ZnF_C2H2	23	45	6.78e-3	SMART
ZnF_C2H2	51	71	2.44e2	SMART
ZnF_C2H2	79	101	3.21e-4	SMART
ZnF_C2H2	131	153	5.59e-4	SMART
ZnF_C2H2	159	181	1.38e-3	SMART
ZnF_C2H2	187	209	9.88e-5	SMART
ZnF_C2H2	215	237	8.47e-4	SMART
ZnF_C2H2	243	265	1.6e-4	SMART
ZnF_C2H2	271	293	8.94e-3	SMART
ZnF_C2H2	299	321	3.21e-4	SMART
ZnF_C2H2	327	349	9.88e-5	SMART
ZnF_C2H2	355	377	1.38e-3	SMART
ZnF_C2H2	383	405	1.38e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000108200
AA Change: K306*

SMART Domains

Protein: ENSMUSP00000103835
Gene: ENSMUSG00000049421
AA Change: K306*

Domain	Start	End	E-Value	Type
ZnF_C2H2	23	45	6.78e-3	SMART
ZnF_C2H2	51	71	2.44e2	SMART
ZnF_C2H2	79	101	3.21e-4	SMART
ZnF_C2H2	131	153	5.59e-4	SMART
ZnF_C2H2	159	181	1.38e-3	SMART
ZnF_C2H2	187	209	9.88e-5	SMART
ZnF_C2H2	215	237	8.47e-4	SMART
ZnF_C2H2	243	265	1.6e-4	SMART
ZnF_C2H2	271	293	8.94e-3	SMART
ZnF_C2H2	299	321	3.21e-4	SMART
ZnF_C2H2	327	349	9.88e-5	SMART
ZnF_C2H2	355	377	1.38e-3	SMART
ZnF_C2H2	383	405	1.38e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000130526

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700024B05Rik	T	C	14: 41,996,173 (GRCm38)	S9P	probably damaging	Het
AI661453	A	T	17: 47,468,406 (GRCm38)	Q1019L	unknown	Het
Aldh1a1	T	A	19: 20,617,937 (GRCm38)	W77R	probably damaging	Het
Cc2d2a	T	C	5: 43,739,309 (GRCm38)	I1516T	probably benign	Het
Ccdc149	T	A	5: 52,405,094 (GRCm38)	I197F	probably damaging	Het
Ccdc189	A	G	7: 127,587,953 (GRCm38)	M46T	probably benign	Het
Cfb	A	G	17: 34,860,891 (GRCm38)	V176A	probably benign	Het
Chordc1	A	G	9: 18,302,101 (GRCm38)	K83E	probably benign	Het
Cic	G	A	7: 25,271,959 (GRCm38)	V372M	probably benign	Het
Coq2	T	A	5: 100,663,875 (GRCm38)		probably benign	Het
Cradd	A	G	10: 95,322,711 (GRCm38)	L58P	probably damaging	Het
Crim1	A	T	17: 78,303,064 (GRCm38)	D316V	probably damaging	Het
Cyb5rl	C	A	4: 107,071,008 (GRCm38)	L114I	possibly damaging	Het
Cyp2c69	T	G	19: 39,877,803 (GRCm38)		probably null	Het
Ddhd1	A	G	14: 45,657,470 (GRCm38)	F181S	probably damaging	Het
Dennd1b	G	A	1: 139,062,873 (GRCm38)	E192K	probably damaging	Het
Dmrt1	T	C	19: 25,506,019 (GRCm38)	S56P	possibly damaging	Het
Dnah12	A	G	14: 26,856,542 (GRCm38)	E3086G	possibly damaging	Het
Dnah5	A	T	15: 28,453,222 (GRCm38)	M4380L	probably benign	Het
Dph7	A	T	2: 24,971,612 (GRCm38)	M346L	probably benign	Het
Dusp27	T	A	1: 166,099,896 (GRCm38)	N716Y	possibly damaging	Het
Egf	C	T	3: 129,735,840 (GRCm38)	R307H	probably benign	Het
Fam50b	G	A	13: 34,747,101 (GRCm38)	E187K	possibly damaging	Het
Fcna	T	C	2: 25,626,286 (GRCm38)	K112E	probably benign	Het
Fkbp5	A	T	17: 28,429,239 (GRCm38)	N125K	possibly damaging	Het
Foxo3	C	T	10: 42,197,769 (GRCm38)	V251I	possibly damaging	Het
Fryl	T	A	5: 73,101,807 (GRCm38)		probably null	Het
Fsip2	A	G	2: 82,951,021 (GRCm38)	E253G	possibly damaging	Het
Gm49333	A	G	16: 20,637,692 (GRCm38)	E428G	possibly damaging	Het
Gprc6a	T	A	10: 51,614,930 (GRCm38)	T908S	probably benign	Het
Grin2c	A	T	11: 115,254,144 (GRCm38)	F560L	probably benign	Het
Hao1	T	A	2: 134,548,252 (GRCm38)	N56Y	probably damaging	Het
Haus3	T	C	5: 34,167,702 (GRCm38)	I204M	probably benign	Het
Htr3b	C	A	9: 48,937,156 (GRCm38)	C263F	probably damaging	Het
Ica1	A	T	6: 8,742,274 (GRCm38)	C120S	probably benign	Het
Inpp4a	T	A	1: 37,367,805 (GRCm38)	S210T	probably benign	Het
Inpp5d	T	G	1: 87,705,949 (GRCm38)	I699S	probably damaging	Het
Kcng1	A	G	2: 168,262,984 (GRCm38)	V314A	probably benign	Het
Lcn11	G	A	2: 25,779,331 (GRCm38)	V167M	possibly damaging	Het
Lingo3	C	A	10: 80,834,548 (GRCm38)	R516L	probably benign	Het
Lrmda	T	C	14: 22,596,478 (GRCm38)	V151A	probably damaging	Het
Ly6g5b	A	C	17: 35,114,602 (GRCm38)	I133R	probably damaging	Het
Mcidas	G	A	13: 112,998,987 (GRCm38)	G315S	probably damaging	Het
Miip	C	T	4: 147,862,918 (GRCm38)	G236S	probably benign	Het
Mroh7	T	C	4: 106,707,576 (GRCm38)	T562A	probably benign	Het
Nlrc4	A	G	17: 74,427,119 (GRCm38)	M933T	probably benign	Het
Olfr539	A	T	7: 140,667,901 (GRCm38)	S198C	possibly damaging	Het
Pkhd1	A	T	1: 20,275,535 (GRCm38)	D2756E	probably damaging	Het
Plekhm2	T	C	4: 141,632,121 (GRCm38)	D445G	probably damaging	Het
Ppp1r12c	C	T	7: 4,483,355 (GRCm38)	G605D	probably benign	Het
Pygb	T	A	2: 150,787,002 (GRCm38)	N45K	possibly damaging	Het
Scn4b	A	G	9: 45,146,771 (GRCm38)	T54A	probably damaging	Het
Scrt1	T	A	15: 76,519,217 (GRCm38)	H191L	unknown	Het
Sdc3	A	G	4: 130,819,196 (GRCm38)	M289V	probably benign	Het
Shb	C	T	4: 45,489,054 (GRCm38)		probably null	Het
Slc1a5	A	G	7: 16,793,870 (GRCm38)	T364A	probably damaging	Het
Slc25a27	A	G	17: 43,649,673 (GRCm38)	V218A	probably benign	Het
Slc39a4	C	T	15: 76,614,085 (GRCm38)	G384D	probably damaging	Het
Sptbn2	C	G	19: 4,749,012 (GRCm38)	R2037G	probably benign	Het
Tmem67	A	T	4: 12,089,284 (GRCm38)		probably null	Het
Tonsl	C	T	15: 76,634,587 (GRCm38)	R544H	probably damaging	Het
Trex1	A	G	9: 109,058,089 (GRCm38)	V278A	unknown	Het
Ttn	A	T	2: 76,795,157 (GRCm38)	D15107E	probably damaging	Het
Tubgcp5	A	G	7: 55,816,562 (GRCm38)	T621A	probably benign	Het
Zfhx4	T	C	3: 5,400,455 (GRCm38)	V1916A	possibly damaging	Het
Zkscan7	A	T	9: 122,895,909 (GRCm38)	T648S	probably benign	Het
Zscan4c	T	A	7: 11,009,772 (GRCm38)	F433I	possibly damaging	Het

Other mutations in Zfp260

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1739:Zfp260	UTSW	7	30,104,806 (GRCm38)	missense	probably benign	0.04
R2143:Zfp260	UTSW	7	30,105,340 (GRCm38)	missense	probably damaging	1.00
R2144:Zfp260	UTSW	7	30,105,340 (GRCm38)	missense	probably damaging	1.00
R2145:Zfp260	UTSW	7	30,105,340 (GRCm38)	missense	probably damaging	1.00
R2400:Zfp260	UTSW	7	30,104,701 (GRCm38)	missense	possibly damaging	0.83
R3802:Zfp260	UTSW	7	30,105,079 (GRCm38)	missense	probably benign	0.01
R5250:Zfp260	UTSW	7	30,104,967 (GRCm38)	missense	probably damaging	1.00
R6313:Zfp260	UTSW	7	30,104,842 (GRCm38)	missense	possibly damaging	0.86
R6416:Zfp260	UTSW	7	30,104,810 (GRCm38)	missense	possibly damaging	0.58
R7369:Zfp260	UTSW	7	30,105,325 (GRCm38)	missense	probably damaging	1.00
R7507:Zfp260	UTSW	7	30,104,866 (GRCm38)	missense	probably damaging	0.99
R8531:Zfp260	UTSW	7	30,105,459 (GRCm38)	missense	probably damaging	1.00
R8868:Zfp260	UTSW	7	30,105,489 (GRCm38)	nonsense	probably null
R9304:Zfp260	UTSW	7	30,104,854 (GRCm38)	missense	possibly damaging	0.55
R9372:Zfp260	UTSW	7	30,104,807 (GRCm38)	missense	probably benign	0.15
R9443:Zfp260	UTSW	7	30,104,824 (GRCm38)	missense	probably benign	0.00
R9579:Zfp260	UTSW	7	30,105,683 (GRCm38)	missense
R9801:Zfp260	UTSW	7	30,105,512 (GRCm38)	missense	possibly damaging	0.49
Z1186:Zfp260	UTSW	7	30,105,038 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TCCCATGACAACCACCGTTAGAA -3'
(R):5'- ATGGGACTTCTGGCTGAAAG -3'

Sequencing Primer
(F):5'- TCCATACTGGAGAGAAGCCTTATG -3'
(R):5'- TTGAGAACTGAGAAAAGGCCTTCCC -3'

Posted On

2020-09-15