Mutagenetix > Incidental Mutation

Incidental Mutation 'R7937:Syt10'

648829

Institutional Source

Beutler Lab

Gene Symbol

Syt10

Ensembl Gene

ENSMUSG00000063260

Gene Name

synaptotagmin X

Synonyms

MMRRC Submission

045983-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.081) question?

Stock #

R7937 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

89666596-89726063 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 89666820 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Arginine at position 510 (S510R)

Ref Sequence

ENSEMBL: ENSMUSP00000029441 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029441]

AlphaFold

Q9R0N4

Predicted Effect

probably damaging
Transcript: ENSMUST00000029441
AA Change: S510R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000029441
Gene: ENSMUSG00000063260
AA Change: S510R

Domain	Start	End	E-Value	Type
transmembrane domain	55	77	N/A	INTRINSIC
low complexity region	105	126	N/A	INTRINSIC
C2	247	350	1.22e-19	SMART
C2	379	493	7.73e-22	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-in allele exhibit minor circadian rhythm impairments. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb11	GTTGATCCATACA	G	2: 69,154,217 (GRCm39)		probably benign	Het
Ano6	C	A	15: 95,870,470 (GRCm39)	T875K	probably damaging	Het
Armc8	G	A	9: 99,418,272 (GRCm39)	T94I	probably damaging	Het
Ash1l	C	T	3: 88,977,624 (GRCm39)	R2685*	probably null	Het
Bri3bp	A	T	5: 125,531,395 (GRCm39)	N114Y	probably damaging	Het
Camkk2	G	T	5: 122,902,097 (GRCm39)	Q71K	probably benign	Het
Cc2d2b	T	C	19: 40,765,736 (GRCm39)	F49S		Het
Cdh10	A	G	15: 18,964,335 (GRCm39)	T166A	probably benign	Het
Chd8	A	G	14: 52,464,963 (GRCm39)	F633L	probably benign	Het
Clec2m	A	G	6: 129,307,974 (GRCm39)	W32R	possibly damaging	Het
Cntn5	G	A	9: 9,748,450 (GRCm39)	T477I	probably damaging	Het
Cyp2c37	T	A	19: 39,982,202 (GRCm39)	Y68N	probably damaging	Het
Dnah2	A	T	11: 69,408,511 (GRCm39)	L53*	probably null	Het
Dnajc11	A	G	4: 152,034,909 (GRCm39)	D44G	probably damaging	Het
Elovl3	T	C	19: 46,123,168 (GRCm39)	F248S	probably damaging	Het
Etfdh	A	G	3: 79,517,123 (GRCm39)	L422P	probably benign	Het
Fam184a	C	A	10: 53,509,802 (GRCm39)	E126*	probably null	Het
Fgd4	A	G	16: 16,287,637 (GRCm39)	Y355H	probably damaging	Het
Fgfr2	A	G	7: 129,820,823 (GRCm39)	M237T	probably damaging	Het
Gdap1	A	T	1: 17,230,177 (GRCm39)	K203N	probably benign	Het
Glis1	A	G	4: 107,484,723 (GRCm39)	D594G	possibly damaging	Het
Gm4787	T	A	12: 81,424,679 (GRCm39)	H493L	probably benign	Het
Gpatch3	G	T	4: 133,310,308 (GRCm39)	V418L	probably damaging	Het
Greb1	T	C	12: 16,766,670 (GRCm39)	N376S	probably damaging	Het
Hspg2	A	G	4: 137,278,243 (GRCm39)	Q3010R	probably benign	Het
Ice2	T	A	9: 69,318,067 (GRCm39)	F223I	possibly damaging	Het
Marchf11	A	G	15: 26,409,323 (GRCm39)	T341A	probably damaging	Het
Mllt10	A	G	2: 18,210,895 (GRCm39)	K770E	probably damaging	Het
Mug1	A	G	6: 121,838,128 (GRCm39)	T453A	probably benign	Het
Myh15	C	T	16: 48,976,009 (GRCm39)	T1359I	probably benign	Het
Nbn	A	G	4: 15,958,080 (GRCm39)	K3E	probably damaging	Het
Nlrp4a	T	C	7: 26,163,571 (GRCm39)	F913L	probably benign	Het
Nlrx1	G	T	9: 44,176,086 (GRCm39)	A48D	probably damaging	Het
Noc3l	C	G	19: 38,783,447 (GRCm39)	G643A	possibly damaging	Het
Or4f14b	T	C	2: 111,774,875 (GRCm39)	R309G	probably benign	Het
Or8c17	T	A	9: 38,180,344 (GRCm39)	N170K	probably benign	Het
Pde6b	G	T	5: 108,567,639 (GRCm39)		probably null	Het
Phactr1	A	G	13: 43,231,205 (GRCm39)	I247V	unknown	Het
Pid1	G	T	1: 84,093,745 (GRCm39)	Q48K	probably benign	Het
Ppfia2	A	G	10: 106,699,233 (GRCm39)	N794S	probably benign	Het
Ppox	G	A	1: 171,107,546 (GRCm39)	S123L	possibly damaging	Het
Ptprd	A	C	4: 76,013,772 (GRCm39)	D778E	probably benign	Het
Rgs13	A	G	1: 144,016,600 (GRCm39)	Y48H	probably damaging	Het
Rgs17	T	A	10: 5,783,078 (GRCm39)	M190L	probably benign	Het
Rictor	C	T	15: 6,801,635 (GRCm39)	S441L	probably benign	Het
Scaf8	C	T	17: 3,247,482 (GRCm39)	P935L	probably damaging	Het
Slc22a6	T	C	19: 8,601,253 (GRCm39)	I435T	probably benign	Het
Slc34a1	A	G	13: 23,996,648 (GRCm39)	H51R	probably benign	Het
Snx4	T	C	16: 33,112,199 (GRCm39)	L378P	probably damaging	Het
Spns1	A	G	7: 125,973,226 (GRCm39)	L155P	probably damaging	Het
St8sia4	T	C	1: 95,581,320 (GRCm39)	T141A	possibly damaging	Het
Tmprss11d	A	T	5: 86,457,349 (GRCm39)	F241L	probably benign	Het
Tnfrsf21	A	T	17: 43,348,816 (GRCm39)	T143S	probably benign	Het
Trim72	A	G	7: 127,609,491 (GRCm39)	N431S	probably benign	Het
Usp43	A	G	11: 67,746,615 (GRCm39)	S1031P	probably damaging	Het
Wdr75	A	G	1: 45,858,799 (GRCm39)	Y656C	probably benign	Het
Zfp28	T	C	7: 6,396,785 (GRCm39)	C407R	probably damaging	Het
Zfp804b	C	T	5: 6,821,866 (GRCm39)	R399Q	possibly damaging	Het
Zfp949	T	A	9: 88,451,323 (GRCm39)	C298S	probably damaging	Het

Other mutations in Syt10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02718:Syt10	APN	15	89,698,282 (GRCm39)	missense	probably damaging	1.00
IGL02976:Syt10	APN	15	89,698,682 (GRCm39)	missense	probably benign	0.26
R0200:Syt10	UTSW	15	89,711,144 (GRCm39)	missense	probably benign	0.01
R0306:Syt10	UTSW	15	89,711,191 (GRCm39)	missense	probably benign	0.02
R0580:Syt10	UTSW	15	89,711,379 (GRCm39)	missense	probably benign	0.15
R0608:Syt10	UTSW	15	89,711,144 (GRCm39)	missense	probably benign	0.01
R1705:Syt10	UTSW	15	89,674,979 (GRCm39)	missense	probably damaging	1.00
R1706:Syt10	UTSW	15	89,674,979 (GRCm39)	missense	probably damaging	1.00
R1921:Syt10	UTSW	15	89,674,979 (GRCm39)	missense	probably damaging	1.00
R1922:Syt10	UTSW	15	89,674,979 (GRCm39)	missense	probably damaging	1.00
R2072:Syt10	UTSW	15	89,674,979 (GRCm39)	missense	probably damaging	1.00
R2074:Syt10	UTSW	15	89,674,979 (GRCm39)	missense	probably damaging	1.00
R2119:Syt10	UTSW	15	89,674,979 (GRCm39)	missense	probably damaging	1.00
R2120:Syt10	UTSW	15	89,674,979 (GRCm39)	missense	probably damaging	1.00
R2121:Syt10	UTSW	15	89,674,979 (GRCm39)	missense	probably damaging	1.00
R3812:Syt10	UTSW	15	89,675,000 (GRCm39)	missense	probably benign
R4029:Syt10	UTSW	15	89,698,741 (GRCm39)	missense	probably benign
R4270:Syt10	UTSW	15	89,675,095 (GRCm39)	missense	probably benign	0.39
R4536:Syt10	UTSW	15	89,666,825 (GRCm39)	missense	probably damaging	0.99
R5333:Syt10	UTSW	15	89,725,932 (GRCm39)	missense	probably benign	0.00
R6042:Syt10	UTSW	15	89,725,824 (GRCm39)	missense	probably benign	0.13
R6104:Syt10	UTSW	15	89,711,067 (GRCm39)	missense	probably benign	0.02
R6445:Syt10	UTSW	15	89,698,471 (GRCm39)	missense	probably damaging	1.00
R6470:Syt10	UTSW	15	89,676,804 (GRCm39)	missense	probably damaging	1.00
R6472:Syt10	UTSW	15	89,698,761 (GRCm39)	missense	probably benign
R6679:Syt10	UTSW	15	89,698,574 (GRCm39)	missense	probably damaging	1.00
R7048:Syt10	UTSW	15	89,675,008 (GRCm39)	missense	probably damaging	1.00
R7128:Syt10	UTSW	15	89,698,314 (GRCm39)	missense	probably damaging	1.00
R7315:Syt10	UTSW	15	89,698,541 (GRCm39)	missense	probably damaging	0.99
R7352:Syt10	UTSW	15	89,698,659 (GRCm39)	missense	probably benign	0.42
R7686:Syt10	UTSW	15	89,698,360 (GRCm39)	missense	probably damaging	1.00
R7789:Syt10	UTSW	15	89,711,101 (GRCm39)	missense	probably damaging	1.00
R8532:Syt10	UTSW	15	89,676,889 (GRCm39)	missense	probably damaging	1.00
R9578:Syt10	UTSW	15	89,675,122 (GRCm39)	missense	possibly damaging	0.80
R9668:Syt10	UTSW	15	89,711,135 (GRCm39)	missense	probably damaging	1.00
X0057:Syt10	UTSW	15	89,711,131 (GRCm39)	missense	probably damaging	1.00
Z1088:Syt10	UTSW	15	89,725,842 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TAAGGGCCATTAGTACTCAAGTAG -3'
(R):5'- CCTTGGAAGGTAAGTTCTGATGAAC -3'

Sequencing Primer
(F):5'- TGCTCAAAGTTGAAGACAAATAAGCC -3'
(R):5'- ACATGGAGGTTACATAGATCATCAC -3'

Posted On

2020-09-15