Mutagenetix > Incidental Mutation

Incidental Mutation 'R0735:Gpr153'

68218

Institutional Source

Beutler Lab

Gene Symbol

Gpr153

Ensembl Gene

ENSMUSG00000042804

Gene Name

G protein-coupled receptor 153

Synonyms

1110065N12Rik, PGR1

MMRRC Submission

038916-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0735 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

152274232-152285337 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 152279373 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 83 (C83*)

Ref Sequence

ENSEMBL: ENSMUSP00000101276 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055754] [ENSMUST00000105650] [ENSMUST00000105651]

AlphaFold

Q8K0Z9

Predicted Effect

probably null
Transcript: ENSMUST00000055754
AA Change: C83*

SMART Domains

Protein: ENSMUSP00000052742
Gene: ENSMUSG00000042804
AA Change: C83*

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	298	1.2e-14	PFAM
low complexity region	501	518	N/A	INTRINSIC
low complexity region	605	617	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000105650
AA Change: C83*

SMART Domains

Protein: ENSMUSP00000101275
Gene: ENSMUSG00000042804
AA Change: C83*

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	297	5.4e-18	PFAM
low complexity region	478	495	N/A	INTRINSIC
low complexity region	582	594	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000105651
AA Change: C83*

SMART Domains

Protein: ENSMUSP00000101276
Gene: ENSMUSG00000042804
AA Change: C83*

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	297	5.3e-17	PFAM
low complexity region	501	518	N/A	INTRINSIC
low complexity region	605	617	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000144035

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.5%
3x: 98.7%
10x: 96.7%
20x: 91.8%

Validation Efficiency

95% (73/77)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an integral membrane protein that belongs to the Class A rhodopsin superfamily of G protein coupled receptors. The encoded protein is expressed primarily in the central nervous system. A knockdown of the orthologous gene in rat is associated with a significant reduction in food intake and impaired decision making ability. Mutations in this gene are associated with schizophrenia, autism, and other neuropsychiatric disorders. The expression of this gene is activated by the glioma-associated oncogene homolog 1 transcription factor which, in turn, is activated by sonic hedgehog in normal and tumorigenic cells. [provided by RefSeq, Feb 2017]

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930021J03Rik	A	T	19: 29,717,638	I1552K	possibly damaging	Het
Actr8	T	A	14: 29,989,712	M405K	probably benign	Het
Adam10	G	A	9: 70,748,251	V334I	possibly damaging	Het
Adgra2	G	T	8: 27,117,318	G686C	probably damaging	Het
Akap11	A	T	14: 78,510,078	I1623N	probably damaging	Het
Astn1	A	T	1: 158,472,389	T100S	possibly damaging	Het
B3galt1	A	C	2: 68,118,579	I213L	possibly damaging	Het
B4galnt4	A	G	7: 141,064,323	K101E	probably benign	Het
Camsap2	A	G	1: 136,292,888	S324P	probably damaging	Het
Chrnb4	A	G	9: 55,043,800	S60P	probably damaging	Het
Cpne1	A	G	2: 156,078,750		probably null	Het
Cubn	G	A	2: 13,491,689		probably benign	Het
Cul7	T	A	17: 46,663,190	L1467H	probably damaging	Het
Cxcl15	T	C	5: 90,801,294	M106T	probably benign	Het
Cyp2c23	A	T	19: 44,016,810	M140K	probably damaging	Het
Dgke	A	G	11: 89,060,075	F104S	probably benign	Het
Dhx36	T	A	3: 62,472,729	M849L	probably benign	Het
Dnah7a	C	T	1: 53,544,511	E1522K	possibly damaging	Het
Edil3	G	T	13: 89,177,178	V219F	probably damaging	Het
Egln1	A	G	8: 124,948,495	V187A	possibly damaging	Het
Fam193a	T	C	5: 34,439,378	I455T	possibly damaging	Het
Fdft1	A	T	14: 63,163,420	I88N	probably damaging	Het
Fem1c	G	A	18: 46,505,160	R592C	probably benign	Het
Frs2	T	A	10: 117,074,582	S292C	probably damaging	Het
Gm15448	T	C	7: 3,821,782	T533A	possibly damaging	Het
Gpr107	T	A	2: 31,171,994	F145I	probably benign	Het
H2-Q7	T	G	17: 35,440,186		probably null	Het
Hsp90b1	A	T	10: 86,695,748		probably benign	Het
Kcnk1	C	A	8: 126,025,289	N211K	probably damaging	Het
Klb	T	C	5: 65,379,727	V800A	probably benign	Het
Lat2	T	C	5: 134,606,783	Y59C	probably damaging	Het
Mlkl	A	T	8: 111,327,801		probably benign	Het
Mroh2a	G	A	1: 88,243,950	R770Q	probably damaging	Het
Mtbp	T	A	15: 55,562,942	C93*	probably null	Het
Myo7a	A	G	7: 98,081,180		probably benign	Het
Myt1	G	A	2: 181,807,387		probably benign	Het
Ogfrl1	T	C	1: 23,375,754	Q224R	possibly damaging	Het
Olfr418	A	G	1: 173,271,002	T276A	probably benign	Het
Olfr661	A	T	7: 104,688,819	H268L	probably damaging	Het
Osbpl2	A	G	2: 180,150,290		probably benign	Het
Plb1	C	T	5: 32,284,920	T252M	possibly damaging	Het
Ptpro	T	A	6: 137,443,594	V1007D	probably damaging	Het
Rbsn	T	C	6: 92,189,693	T657A	probably benign	Het
Rims4	C	T	2: 163,863,929	V262M	possibly damaging	Het
Rps6kb2	C	A	19: 4,157,883	S348I	probably benign	Het
Rsrp1	C	T	4: 134,924,257	R111W	unknown	Het
Ryr3	T	C	2: 112,732,982	T2933A	probably benign	Het
Scara5	A	G	14: 65,731,019	D247G	possibly damaging	Het
Slc7a11	C	T	3: 50,424,096	S231N	probably benign	Het
Sod2	A	T	17: 13,010,564	N91Y	probably damaging	Het
Spesp1	A	T	9: 62,272,685	S314T	probably benign	Het
St3gal1	C	A	15: 67,113,687	M39I	probably benign	Het
Stat6	A	T	10: 127,658,241	I646F	probably damaging	Het
Tdrd1	A	T	19: 56,865,978	K1119*	probably null	Het
Thbs2	A	G	17: 14,679,815	I600T	probably benign	Het
Tor1a	A	G	2: 30,963,838	V160A	probably damaging	Het
Trdmt1	T	G	2: 13,523,438	D104A	probably benign	Het
Trim58	T	C	11: 58,651,393	V393A	probably benign	Het
Trip4	C	T	9: 65,884,918		probably benign	Het
Trip6	T	C	5: 137,310,821	E341G	probably benign	Het
Ttn	T	A	2: 76,715,195	I32595F	probably damaging	Het
Ubr4	T	A	4: 139,428,028		probably null	Het
Ush2a	G	A	1: 188,864,693	V3877I	probably benign	Het
Vmn1r29	G	T	6: 58,307,732	G146C	probably damaging	Het
Vmn2r53	A	G	7: 12,581,780	V704A	probably benign	Het
Vmn2r7	C	T	3: 64,716,367	M268I	probably benign	Het
Wnt7b	G	A	15: 85,537,495	T248M	probably damaging	Het
Xab2	G	A	8: 3,613,649	P394S	possibly damaging	Het
Zfp663	A	G	2: 165,359,075	V13A	probably damaging	Het

Other mutations in Gpr153

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01350:Gpr153	APN	4	152281966	unclassified	probably benign
IGL01368:Gpr153	APN	4	152282994	missense	probably benign	0.40
IGL01568:Gpr153	APN	4	152282368	splice site	probably null
IGL01672:Gpr153	APN	4	152279913	nonsense	probably null
R0925:Gpr153	UTSW	4	152281874	missense	probably benign
R1302:Gpr153	UTSW	4	152279943	missense	probably damaging	1.00
R1829:Gpr153	UTSW	4	152282392	missense	possibly damaging	0.70
R2041:Gpr153	UTSW	4	152283353	missense	probably benign
R4698:Gpr153	UTSW	4	152281783	missense	probably damaging	1.00
R5069:Gpr153	UTSW	4	152279883	missense	probably damaging	0.99
R5623:Gpr153	UTSW	4	152281941	missense	possibly damaging	0.89
R5800:Gpr153	UTSW	4	152280077	nonsense	probably null
R5940:Gpr153	UTSW	4	152283375	missense	probably benign	0.12
R6773:Gpr153	UTSW	4	152279300	missense	probably damaging	1.00
R6944:Gpr153	UTSW	4	152279363	missense	probably damaging	1.00
R7486:Gpr153	UTSW	4	152282401	missense	probably benign	0.01
R8170:Gpr153	UTSW	4	152280177	missense	probably damaging	1.00
R8699:Gpr153	UTSW	4	152279101	start gained	probably benign
R8701:Gpr153	UTSW	4	152279101	start gained	probably benign
R8732:Gpr153	UTSW	4	152279101	start gained	probably benign
R9047:Gpr153	UTSW	4	152280207	missense	probably damaging	1.00
R9383:Gpr153	UTSW	4	152283059	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CAGTCTGCACCATGAGTGATGAGC -3'
(R):5'- ATGCCTTTGCCTCAAGCACAGC -3'

Sequencing Primer
(F):5'- CATGCGGAGGTCTGTCG -3'
(R):5'- CTCAAGCACAGCCCCTC -3'

Posted On

2013-09-03