Incidental Mutation 'R9495:Ptgr2'
ID 717178
Institutional Source Beutler Lab
Gene Symbol Ptgr2
Ensembl Gene ENSMUSG00000072946
Gene Name prostaglandin reductase 2
Synonyms Zadh1, 9630002F03Rik, B830026H24Rik, 1810016I24Rik, PGR-2
MMRRC Submission
Accession Numbers
Essential gene? Probably non essential (E-score: 0.166) question?
Stock # R9495 (G1)
Quality Score 225.009
Status Not validated
Chromosome 12
Chromosomal Location 84332011-84362606 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 84354647 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Threonine at position 276 (I276T)
Ref Sequence ENSEMBL: ENSMUSP00000115704 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000123614] [ENSMUST00000123693] [ENSMUST00000135001] [ENSMUST00000146377] [ENSMUST00000147363]
AlphaFold Q8VDQ1
PDB Structure Crystal structure of putative NADPH-dependent oxidoreductase from Mus musculus at 2.10 A resolution [X-RAY DIFFRACTION]
Crystal structure of mouse 15-ketoprostaglandin delta-13-reductase in complex with NADPH [X-RAY DIFFRACTION]
Predicted Effect probably benign
Transcript: ENSMUST00000123614
AA Change: I276T

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)
SMART Domains Protein: ENSMUSP00000115704
Gene: ENSMUSG00000072946
AA Change: I276T

Pfam:ADH_N_2 4 165 5.4e-40 PFAM
Pfam:ADH_zinc_N 166 287 1.3e-15 PFAM
Pfam:ADH_zinc_N_2 200 343 4.5e-11 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000123693
SMART Domains Protein: ENSMUSP00000115506
Gene: ENSMUSG00000072946

Pfam:ADH_N_2 4 56 1.3e-12 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000135001
AA Change: I276T

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)
SMART Domains Protein: ENSMUSP00000114559
Gene: ENSMUSG00000072946
AA Change: I276T

Pfam:ADH_zinc_N 166 283 7.4e-19 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000146377
AA Change: I276T

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)
SMART Domains Protein: ENSMUSP00000119981
Gene: ENSMUSG00000072946
AA Change: I276T

Pfam:ADH_zinc_N 166 283 1.4e-16 PFAM
Pfam:ADH_zinc_N_2 200 343 6.9e-9 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000147363
AA Change: I276T

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)
SMART Domains Protein: ENSMUSP00000114766
Gene: ENSMUSG00000072946
AA Change: I276T

Pfam:ADH_zinc_N 166 283 1.2e-18 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an enzyme involved in the metabolism of prostaglandins. The encoded protein catalyzes the NADPH-dependent conversion of 15-keto-prostaglandin E2 to 15-keto-13,14-dihydro-prostaglandin E2. This protein may also be involved in regulating activation of the peroxisome proliferator-activated receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700088E04Rik T A 15: 79,019,842 (GRCm39) D158V probably benign Het
Aadacl2fm1 T C 3: 59,840,114 (GRCm39) I62T possibly damaging Het
Acp5 G A 9: 22,038,483 (GRCm39) Q273* probably null Het
Apoa5 G C 9: 46,181,944 (GRCm39) R340P probably damaging Het
Atp2b1 T A 10: 98,835,660 (GRCm39) N468K probably damaging Het
Bltp3a A G 17: 28,112,414 (GRCm39) D1201G probably damaging Het
Bphl A T 13: 34,234,312 (GRCm39) I143L probably benign Het
Cd33 A T 7: 43,182,150 (GRCm39) H98Q probably benign Het
Celsr1 G T 15: 85,917,286 (GRCm39) S229* probably null Het
Cemip2 T A 19: 21,779,249 (GRCm39) V353D probably damaging Het
Cfap96 C T 8: 46,409,458 (GRCm39) S287N probably damaging Het
Colec10 A G 15: 54,325,761 (GRCm39) D197G probably damaging Het
Cpt1a T C 19: 3,433,795 (GRCm39) M759T probably benign Het
Ctc1 A G 11: 68,913,593 (GRCm39) Y165C probably damaging Het
Cyp2t4 G A 7: 26,854,717 (GRCm39) V66M possibly damaging Het
Dnah2 T C 11: 69,345,208 (GRCm39) D2667G possibly damaging Het
Dok1 T C 6: 83,009,972 (GRCm39) K46E probably damaging Het
Erv3 C A 2: 131,697,975 (GRCm39) W128L possibly damaging Het
Fam186a A G 15: 99,844,766 (GRCm39) S493P unknown Het
Fbn1 A T 2: 125,160,984 (GRCm39) N2185K probably damaging Het
Figla A C 6: 85,997,689 (GRCm39) H139P probably benign Het
Gemin4 A G 11: 76,101,749 (GRCm39) L1004P probably damaging Het
Gm11937 T C 11: 99,500,646 (GRCm39) T124A unknown Het
Gm17669 G T 18: 67,695,682 (GRCm39) V76L probably benign Het
H2ac21 A G 3: 96,127,401 (GRCm39) E57G probably damaging Het
Hcls1 A T 16: 36,777,702 (GRCm39) M274L probably benign Het
Il17rc T C 6: 113,449,741 (GRCm39) S116P probably damaging Het
Krt79 G A 15: 101,840,288 (GRCm39) R303C probably damaging Het
Lamb2 C T 9: 108,358,006 (GRCm39) T149I probably damaging Het
Lrrc41 T A 4: 115,932,806 (GRCm39) probably null Het
Mga C A 2: 119,781,676 (GRCm39) T2234K possibly damaging Het
Mix23 A G 16: 35,892,491 (GRCm39) E12G probably benign Het
Muc6 T A 7: 141,237,398 (GRCm39) Q205L probably damaging Het
Nlrp9b A T 7: 19,760,462 (GRCm39) K624N possibly damaging Het
Or1o4 G T 17: 37,591,386 (GRCm39) probably benign Het
Or4d2 C T 11: 87,784,082 (GRCm39) V223M probably benign Het
Or6e1 T C 14: 54,520,137 (GRCm39) T72A probably damaging Het
Otud4 T C 8: 80,400,087 (GRCm39) S934P probably damaging Het
Pcdha12 G T 18: 37,155,526 (GRCm39) W748C probably damaging Het
Pdc T C 1: 150,208,919 (GRCm39) I134T probably damaging Het
Peg10 T TCCC 6: 4,756,451 (GRCm39) probably benign Het
Pkn1 C T 8: 84,410,799 (GRCm39) R276Q possibly damaging Het
Plin3 C A 17: 56,587,824 (GRCm39) G297V probably benign Het
Podn C T 4: 107,876,106 (GRCm39) V517I probably benign Het
Pomk T C 8: 26,473,344 (GRCm39) D203G probably damaging Het
Ppil4 A G 10: 7,675,355 (GRCm39) D168G probably damaging Het
Ptk7 T A 17: 46,887,744 (GRCm39) I563F possibly damaging Het
Rbm12 G T 2: 155,939,738 (GRCm39) T178K unknown Het
Sctr T C 1: 119,959,403 (GRCm39) probably null Het
Sh2b1 GGGACC GGGACCGGCTCAGCCACGTGGACC 7: 126,066,744 (GRCm39) probably benign Het
Spata31e4 T A 13: 50,855,465 (GRCm39) S368T possibly damaging Het
Steap1 A T 5: 5,786,458 (GRCm39) D326E probably damaging Het
Stra6 G A 9: 58,059,175 (GRCm39) V513I probably benign Het
Tbx19 A G 1: 164,966,546 (GRCm39) S443P unknown Het
Tcea3 T A 4: 135,991,885 (GRCm39) C190S probably damaging Het
Tfr2 G A 5: 137,572,701 (GRCm39) V171I probably benign Het
Tm7sf3 C T 6: 146,525,179 (GRCm39) D89N possibly damaging Het
Tmem129 A G 5: 33,815,122 (GRCm39) V17A probably benign Het
Tmem87b T C 2: 128,660,353 (GRCm39) L32P probably damaging Het
Tor1aip1 T A 1: 155,906,177 (GRCm39) D205V probably damaging Het
Trim33 T C 3: 103,239,074 (GRCm39) V684A probably benign Het
Triobp C T 15: 78,877,378 (GRCm39) R1637C probably damaging Het
Ulbp1 C A 10: 7,406,371 (GRCm39) M196I probably benign Het
Vash1 G A 12: 86,738,663 (GRCm39) G370E probably damaging Het
Vmn2r23 A G 6: 123,689,672 (GRCm39) T183A probably benign Het
Vta1 A G 10: 14,531,583 (GRCm39) I264T probably benign Het
Zcchc8 A T 5: 123,838,633 (GRCm39) M635K probably benign Het
Other mutations in Ptgr2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02382:Ptgr2 APN 12 84,360,722 (GRCm39) missense probably damaging 0.99
IGL03176:Ptgr2 APN 12 84,354,668 (GRCm39) missense probably damaging 1.00
R1914:Ptgr2 UTSW 12 84,349,096 (GRCm39) missense probably benign 0.11
R1915:Ptgr2 UTSW 12 84,349,096 (GRCm39) missense probably benign 0.11
R4624:Ptgr2 UTSW 12 84,355,128 (GRCm39) missense possibly damaging 0.70
R5827:Ptgr2 UTSW 12 84,342,110 (GRCm39) critical splice donor site probably null
R5978:Ptgr2 UTSW 12 84,342,032 (GRCm39) nonsense probably null
R5986:Ptgr2 UTSW 12 84,355,120 (GRCm39) missense possibly damaging 0.85
R6019:Ptgr2 UTSW 12 84,344,920 (GRCm39) missense probably damaging 1.00
R6320:Ptgr2 UTSW 12 84,349,111 (GRCm39) missense probably benign 0.05
R6526:Ptgr2 UTSW 12 84,360,726 (GRCm39) missense probably damaging 1.00
R7369:Ptgr2 UTSW 12 84,339,080 (GRCm39) start gained probably benign
R7401:Ptgr2 UTSW 12 84,339,103 (GRCm39) start gained probably benign
R7463:Ptgr2 UTSW 12 84,339,072 (GRCm39) start gained probably benign
R7583:Ptgr2 UTSW 12 84,355,179 (GRCm39) missense probably damaging 1.00
R7793:Ptgr2 UTSW 12 84,354,575 (GRCm39) missense probably damaging 1.00
R8172:Ptgr2 UTSW 12 84,360,783 (GRCm39) missense possibly damaging 0.93
R9005:Ptgr2 UTSW 12 84,344,873 (GRCm39) missense possibly damaging 0.86
Predicted Primers
Posted On 2022-07-18