|Institutional Source||Beutler Lab|
|Gene Name||interleukin 10|
|Synonyms||IL-10, cytokine synthesis inhibitory factor|
|Is this an essential gene?||Probably essential (E-score: 0.762)|
|Stock #||R2034 (G1)|
|Chromosomal Location||131019845-131024974 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to A at 131024185 bp|
|Amino Acid Change||Glutamine to Lysine at position 152 (Q152K)|
|Ref Sequence||ENSEMBL: ENSMUSP00000016673 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000016673]|
|PDB Structure||X-ray structure of mouse interleukin-10 mutant - S1_E8del, C149Y [X-RAY DIFFRACTION]|
|Predicted Effect||probably benign
AA Change: Q152K
PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
AA Change: Q152K
|Meta Mutation Damage Score||0.0898|
|Coding Region Coverage||
|Validation Efficiency||100% (71/71)|
|MGI Phenotype||Strain: 1857199; 3521569
FUNCTION: This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates it's immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, Sep 2015]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit retarded growth, anemia, chronic enterocolitis, a high incidence of colorectal adenocarcinomas, and altered responses to various infectious organisms such as Mycobacterium bovis and Leishmania donovani. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Il10||
(F):5'- CTTCAAGTCAAGCGTCTTGTTG -3'
(R):5'- TCCGTTAGCTAAGATCCCTGG -3'
(F):5'- TCAAGCGTCTTGTTGTTTAAATAAAC -3'
(R):5'- CCCTGGATCAGATTTAGAGAGCTC -3'