Mutagenetix > Incidental Mutation

Incidental Mutation 'R2111:Myh1'

232573

Institutional Source

Beutler Lab

Gene Symbol

Myh1

Ensembl Gene

ENSMUSG00000056328

Gene Name

myosin, heavy polypeptide 1, skeletal muscle, adult

Synonyms

A530084A17Rik, Myhsf2, Myhs-f2, Myhs-f, IId/x, IId, myosin heavy chain 2X, MYHC-IIX, MyHC-IId/x

MMRRC Submission

040115-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2111 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

67200052-67224575 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 67214620 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Asparagine at position 1079 (D1079N)

Ref Sequence

ENSEMBL: ENSMUSP00000117569 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000018637] [ENSMUST00000075734] [ENSMUST00000124516]

AlphaFold

Q5SX40

Predicted Effect

possibly damaging
Transcript: ENSMUST00000018637
AA Change: D1079N

PolyPhen 2 Score 0.832 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000018637
Gene: ENSMUSG00000056328
AA Change: D1079N

Domain	Start	End	E-Value	Type
Pfam:Myosin_N	35	76	1.8e-15	PFAM
MYSc	80	786	N/A	SMART
IQ	787	809	1.18e-3	SMART
low complexity region	860	883	N/A	INTRINSIC
low complexity region	931	945	N/A	INTRINSIC
Pfam:Myosin_tail_1	1075	1933	N/A	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000075734
AA Change: D1079N

PolyPhen 2 Score 0.832 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000075147
Gene: ENSMUSG00000056328
AA Change: D1079N

Domain	Start	End	E-Value	Type
Pfam:Myosin_N	35	74	7.2e-14	PFAM
MYSc	80	786	N/A	SMART
IQ	787	809	1.18e-3	SMART
Pfam:Myosin_tail_1	850	1931	1.9e-165	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000124516
AA Change: D1079N

PolyPhen 2 Score 0.832 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000117569
Gene: ENSMUSG00000056328
AA Change: D1079N

Domain	Start	End	E-Value	Type
Pfam:Myosin_N	35	76	1.8e-15	PFAM
MYSc	80	786	N/A	SMART
IQ	787	809	1.18e-3	SMART
low complexity region	860	883	N/A	INTRINSIC
low complexity region	931	945	N/A	INTRINSIC
Pfam:Myosin_tail_1	1075	1933	N/A	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145021

Meta Mutation Damage Score

0.1357

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

100% (86/86)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit reduced growth, muscular weakness, kyphosis, and abnormal kinetics of muscle contraction and relaxation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9330161L09Rik	T	C	12: 103,407,589 (GRCm38)		probably benign	Het
Abca2	A	T	2: 25,437,505 (GRCm38)	I669F	possibly damaging	Het
Adam9	C	T	8: 24,982,126 (GRCm38)		probably benign	Het
Adprhl1	A	G	8: 13,248,694 (GRCm38)	Y79H	probably damaging	Het
Akp3	A	T	1: 87,126,885 (GRCm38)		probably null	Het
Alpk2	T	C	18: 65,349,774 (GRCm38)	S388G	probably benign	Het
Amph	G	A	13: 19,116,266 (GRCm38)		probably benign	Het
Arr3	T	A	X: 100,614,641 (GRCm38)	F269L	possibly damaging	Het
Atp2a2	A	G	5: 122,459,546 (GRCm38)	F808S	probably damaging	Het
Baz1a	T	A	12: 54,911,385 (GRCm38)	N1027I	probably damaging	Het
C2cd4c	G	T	10: 79,612,421 (GRCm38)	H297Q	probably damaging	Het
Calr3	T	A	8: 72,427,268 (GRCm38)	D160V	probably damaging	Het
Cdc42ep1	C	T	15: 78,847,492 (GRCm38)	R46C	probably damaging	Het
Cdh23	G	T	10: 60,305,583 (GRCm38)	F3127L	probably damaging	Het
Cps1	T	A	1: 67,176,980 (GRCm38)	D821E	probably benign	Het
Cyp2e1	C	A	7: 140,773,634 (GRCm38)	T328K	probably damaging	Het
Dcp1a	T	C	14: 30,519,370 (GRCm38)	V379A	probably benign	Het
Dst	T	C	1: 34,169,178 (GRCm38)	S737P	probably damaging	Het
Dtx2	C	T	5: 136,030,577 (GRCm38)	S493F	probably damaging	Het
Eci2	A	G	13: 34,990,716 (GRCm38)		probably null	Het
Ercc6l2	A	G	13: 63,834,749 (GRCm38)	T126A	probably damaging	Het
Fgf6	T	C	6: 127,015,760 (GRCm38)	S59P	probably damaging	Het
Fmnl2	A	G	2: 53,105,537 (GRCm38)	E424G	probably damaging	Het
Gapvd1	G	T	2: 34,684,317 (GRCm38)	A1256D	probably benign	Het
Gcfc2	C	A	6: 81,923,778 (GRCm38)	D24E	probably benign	Het
Gigyf2	A	G	1: 87,440,730 (GRCm38)	H1044R	probably damaging	Het
Gk2	T	C	5: 97,456,305 (GRCm38)	I225V	probably benign	Het
Gm3944	C	A	12: 18,853,894 (GRCm38)	S8*	probably null	Het
Gm4788	T	A	1: 139,774,679 (GRCm38)		probably benign	Het
Gnl3l	A	G	X: 150,997,294 (GRCm38)	S217P	probably damaging	Het
Gpr156	A	G	16: 37,978,751 (GRCm38)	D109G	probably benign	Het
Hoxd1	A	T	2: 74,763,366 (GRCm38)	T89S	probably benign	Het
Ift172	G	A	5: 31,286,079 (GRCm38)	T112M	probably benign	Het
Igkv1-115	C	A	6: 68,161,629 (GRCm38)	S72*	probably null	Het
Ildr1	A	G	16: 36,721,979 (GRCm38)	E247G	probably damaging	Het
Insr	A	G	8: 3,169,748 (GRCm38)	S925P	probably benign	Het
Itpr1	T	G	6: 108,378,309 (GRCm38)		probably benign	Het
Khdrbs3	C	T	15: 69,024,824 (GRCm38)	T111I	probably benign	Het
Mageb3	A	G	2: 121,954,825 (GRCm38)		probably null	Het
Mcf2l	A	T	8: 13,001,867 (GRCm38)	K433N	probably damaging	Het
Mdn1	A	G	4: 32,700,409 (GRCm38)	E1456G	probably damaging	Het
Mta1	C	T	12: 113,131,628 (GRCm38)	T467I	probably damaging	Het
Mtr	A	T	13: 12,244,601 (GRCm38)	I196N	possibly damaging	Het
Mtus1	G	T	8: 41,022,571 (GRCm38)	P819T	probably damaging	Het
Nav1	C	T	1: 135,449,004 (GRCm38)	R1694Q	probably damaging	Het
Neb	A	G	2: 52,284,263 (GRCm38)	I1528T	probably benign	Het
Nek1	T	A	8: 61,124,326 (GRCm38)		probably null	Het
Nes	A	G	3: 87,977,311 (GRCm38)	E915G	probably benign	Het
Nlrp4f	T	C	13: 65,199,353 (GRCm38)	I30M	probably benign	Het
Nup153	A	G	13: 46,683,928 (GRCm38)	S1273P	probably benign	Het
Nup155	A	G	15: 8,121,467 (GRCm38)	I334V	probably benign	Het
Olfr1085	G	T	2: 86,658,437 (GRCm38)	T7K	probably damaging	Het
Olfr1170	A	G	2: 88,224,474 (GRCm38)	V186A	possibly damaging	Het
Olfr1469	G	A	19: 13,410,943 (GRCm38)	A125T	probably damaging	Het
Olfr213	T	C	6: 116,540,650 (GRCm38)	Y66H	possibly damaging	Het
Olfr397	A	G	11: 73,964,914 (GRCm38)	Y102C	probably damaging	Het
Olfr418	A	T	1: 173,270,312 (GRCm38)	M46L	probably benign	Het
Olfr910	T	A	9: 38,539,280 (GRCm38)	N128K	probably benign	Het
Parn	G	A	16: 13,603,069 (GRCm38)	S473L	probably damaging	Het
Pcnt	T	C	10: 76,420,526 (GRCm38)	K627E	probably damaging	Het
Pja2	T	A	17: 64,290,036 (GRCm38)	D553V	probably damaging	Het
Prune2	T	C	19: 17,208,238 (GRCm38)	F3004L	probably damaging	Het
Rasgrp3	T	C	17: 75,500,758 (GRCm38)		probably null	Het
Rdh13	A	G	7: 4,445,483 (GRCm38)	V10A	probably benign	Het
Rimbp2	T	C	5: 128,773,501 (GRCm38)	Y906C	probably damaging	Het
Ripor1	T	C	8: 105,614,712 (GRCm38)	F59S	probably damaging	Het
Rnf185	T	C	11: 3,432,393 (GRCm38)		probably benign	Het
Runx3	T	C	4: 135,155,316 (GRCm38)	V107A	probably damaging	Het
Scn3b	T	C	9: 40,282,445 (GRCm38)	V156A	probably benign	Het
Serpina3a	G	A	12: 104,116,222 (GRCm38)	A85T	probably damaging	Het
Slc12a7	C	T	13: 73,785,155 (GRCm38)	R111*	probably null	Het
Slc5a5	A	T	8: 70,889,751 (GRCm38)		probably null	Het
Snx13	A	T	12: 35,138,085 (GRCm38)	L787F	probably damaging	Het
Spef2	A	T	15: 9,589,573 (GRCm38)	M1535K	probably damaging	Het
Sphkap	T	G	1: 83,275,881 (GRCm38)	K1382N	probably benign	Het
Tagap1	A	G	17: 6,956,860 (GRCm38)	S146P	probably benign	Het
Tagln3	A	G	16: 45,711,594 (GRCm38)	Y192H	probably damaging	Het
Tbc1d15	A	C	10: 115,240,914 (GRCm38)	S22A	possibly damaging	Het
Tbc1d31	A	G	15: 57,932,644 (GRCm38)	E211G	probably benign	Het
Tmod3	A	G	9: 75,509,363 (GRCm38)	V229A	probably damaging	Het
Ubr2	A	G	17: 46,963,145 (GRCm38)		probably null	Het
Ugt2b36	T	C	5: 87,092,241 (GRCm38)	E95G	probably benign	Het
Unc45b	G	A	11: 82,911,689 (GRCm38)	A4T	probably benign	Het
Usp40	A	G	1: 87,950,214 (GRCm38)	I1117T	probably benign	Het
Vcan	T	A	13: 89,693,303 (GRCm38)	D414V	probably damaging	Het
Xrn1	C	A	9: 96,039,832 (GRCm38)	H1433N	probably benign	Het
Zfp353-ps	T	A	8: 42,082,968 (GRCm38)		noncoding transcript	Het
Zfp74	G	A	7: 29,935,018 (GRCm38)	Q422*	probably null	Het
Zmym3	A	T	X: 101,407,387 (GRCm38)	V1208D	probably damaging	Het

Other mutations in Myh1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00427:Myh1	APN	11	67,220,865 (GRCm38)	missense	probably damaging	0.99
IGL00514:Myh1	APN	11	67,219,784 (GRCm38)	missense	probably damaging	1.00
IGL00851:Myh1	APN	11	67,217,910 (GRCm38)	missense	probably damaging	0.96
IGL01061:Myh1	APN	11	67,217,862 (GRCm38)	missense	probably benign	0.05
IGL01113:Myh1	APN	11	67,202,180 (GRCm38)	missense	probably benign	0.00
IGL01125:Myh1	APN	11	67,220,660 (GRCm38)	missense	probably benign
IGL01391:Myh1	APN	11	67,217,863 (GRCm38)	missense	probably benign	0.00
IGL01392:Myh1	APN	11	67,221,301 (GRCm38)	missense	probably benign	0.20
IGL01404:Myh1	APN	11	67,222,151 (GRCm38)	missense	possibly damaging	0.83
IGL01700:Myh1	APN	11	67,211,412 (GRCm38)	missense	probably damaging	1.00
IGL01739:Myh1	APN	11	67,214,528 (GRCm38)	missense	probably damaging	0.99
IGL01759:Myh1	APN	11	67,219,906 (GRCm38)	missense	probably damaging	1.00
IGL01922:Myh1	APN	11	67,210,466 (GRCm38)	critical splice donor site	probably null
IGL01952:Myh1	APN	11	67,220,392 (GRCm38)	splice site	probably null
IGL02007:Myh1	APN	11	67,220,556 (GRCm38)	missense	probably benign	0.03
IGL02028:Myh1	APN	11	67,210,615 (GRCm38)	missense	probably damaging	1.00
IGL02245:Myh1	APN	11	67,211,487 (GRCm38)	missense	possibly damaging	0.58
IGL02628:Myh1	APN	11	67,206,262 (GRCm38)	unclassified	probably benign
IGL02942:Myh1	APN	11	67,202,482 (GRCm38)	missense	probably damaging	1.00
IGL02967:Myh1	APN	11	67,209,070 (GRCm38)	missense	possibly damaging	0.76
IGL03031:Myh1	APN	11	67,206,387 (GRCm38)	missense	possibly damaging	0.47
IGL03187:Myh1	APN	11	67,206,525 (GRCm38)	missense	possibly damaging	0.56
IGL03302:Myh1	APN	11	67,211,502 (GRCm38)	missense	probably benign	0.01
compelling	UTSW	11	67,219,805 (GRCm38)	critical splice donor site	probably null
convincing	UTSW	11	67,202,539 (GRCm38)	missense	probably damaging	1.00
muscle	UTSW	11	67,206,048 (GRCm38)	nonsense	probably null
Persuasive	UTSW	11	67,209,064 (GRCm38)	missense	possibly damaging	0.90
G1patch:Myh1	UTSW	11	67,201,893 (GRCm38)	missense	probably damaging	1.00
R0041:Myh1	UTSW	11	67,209,078 (GRCm38)	missense	possibly damaging	0.88
R0079:Myh1	UTSW	11	67,213,411 (GRCm38)	missense	probably damaging	1.00
R0081:Myh1	UTSW	11	67,215,857 (GRCm38)	missense	probably benign
R0317:Myh1	UTSW	11	67,217,512 (GRCm38)	missense	probably damaging	1.00
R0465:Myh1	UTSW	11	67,210,417 (GRCm38)	missense	possibly damaging	0.50
R0528:Myh1	UTSW	11	67,220,619 (GRCm38)	missense	probably damaging	1.00
R0731:Myh1	UTSW	11	67,202,533 (GRCm38)	missense	probably damaging	0.98
R0964:Myh1	UTSW	11	67,205,925 (GRCm38)	missense	probably benign
R0964:Myh1	UTSW	11	67,221,604 (GRCm38)	missense	probably damaging	1.00
R1427:Myh1	UTSW	11	67,219,747 (GRCm38)	missense	probably damaging	0.99
R1429:Myh1	UTSW	11	67,217,910 (GRCm38)	missense	possibly damaging	0.78
R1481:Myh1	UTSW	11	67,205,499 (GRCm38)	unclassified	probably benign
R1562:Myh1	UTSW	11	67,211,370 (GRCm38)	missense	probably benign	0.04
R1727:Myh1	UTSW	11	67,210,466 (GRCm38)	critical splice donor site	probably benign
R1796:Myh1	UTSW	11	67,224,357 (GRCm38)	missense	probably benign	0.00
R1808:Myh1	UTSW	11	67,211,474 (GRCm38)	nonsense	probably null
R1836:Myh1	UTSW	11	67,204,822 (GRCm38)	missense	probably damaging	0.98
R1848:Myh1	UTSW	11	67,213,630 (GRCm38)	missense	probably benign	0.10
R1851:Myh1	UTSW	11	67,204,398 (GRCm38)	missense	probably damaging	1.00
R1925:Myh1	UTSW	11	67,211,170 (GRCm38)	missense	probably benign	0.01
R1967:Myh1	UTSW	11	67,213,447 (GRCm38)	missense	probably benign	0.08
R1999:Myh1	UTSW	11	67,222,408 (GRCm38)	missense	probably benign	0.04
R2067:Myh1	UTSW	11	67,214,620 (GRCm38)	missense	possibly damaging	0.83
R2150:Myh1	UTSW	11	67,222,408 (GRCm38)	missense	probably benign	0.04
R2189:Myh1	UTSW	11	67,221,604 (GRCm38)	missense	probably damaging	1.00
R2352:Myh1	UTSW	11	67,220,537 (GRCm38)	missense	probably benign	0.00
R2436:Myh1	UTSW	11	67,213,271 (GRCm38)	missense	probably benign	0.04
R2483:Myh1	UTSW	11	67,211,226 (GRCm38)	missense	probably benign
R2508:Myh1	UTSW	11	67,213,598 (GRCm38)	missense	possibly damaging	0.61
R2509:Myh1	UTSW	11	67,205,597 (GRCm38)	missense	probably benign	0.01
R2511:Myh1	UTSW	11	67,205,597 (GRCm38)	missense	probably benign	0.01
R2908:Myh1	UTSW	11	67,220,696 (GRCm38)	nonsense	probably null
R2966:Myh1	UTSW	11	67,214,584 (GRCm38)	missense	probably damaging	1.00
R3829:Myh1	UTSW	11	67,205,597 (GRCm38)	missense	probably benign	0.01
R4106:Myh1	UTSW	11	67,211,577 (GRCm38)	missense	probably benign	0.33
R4108:Myh1	UTSW	11	67,211,577 (GRCm38)	missense	probably benign	0.33
R4457:Myh1	UTSW	11	67,220,615 (GRCm38)	missense	probably benign	0.42
R4629:Myh1	UTSW	11	67,209,293 (GRCm38)	missense	probably benign	0.01
R4981:Myh1	UTSW	11	67,224,474 (GRCm38)	utr 3 prime	probably benign
R5032:Myh1	UTSW	11	67,206,048 (GRCm38)	nonsense	probably null
R5239:Myh1	UTSW	11	67,215,225 (GRCm38)	missense	probably benign	0.19
R5241:Myh1	UTSW	11	67,204,449 (GRCm38)	missense	probably benign
R5303:Myh1	UTSW	11	67,202,017 (GRCm38)	missense	probably benign	0.09
R5666:Myh1	UTSW	11	67,221,352 (GRCm38)	missense	probably benign	0.30
R5717:Myh1	UTSW	11	67,208,956 (GRCm38)	missense	probably benign
R5761:Myh1	UTSW	11	67,219,252 (GRCm38)	missense	probably damaging	0.98
R5870:Myh1	UTSW	11	67,201,979 (GRCm38)	missense	possibly damaging	0.70
R6077:Myh1	UTSW	11	67,211,447 (GRCm38)	missense	probably damaging	1.00
R6089:Myh1	UTSW	11	67,220,787 (GRCm38)	splice site	probably null
R6089:Myh1	UTSW	11	67,202,167 (GRCm38)	splice site	probably null
R6197:Myh1	UTSW	11	67,220,967 (GRCm38)	missense	probably benign	0.01
R6460:Myh1	UTSW	11	67,221,376 (GRCm38)	missense	probably benign
R6627:Myh1	UTSW	11	67,215,009 (GRCm38)	missense	probably damaging	1.00
R6634:Myh1	UTSW	11	67,209,064 (GRCm38)	missense	possibly damaging	0.90
R6725:Myh1	UTSW	11	67,201,893 (GRCm38)	missense	probably damaging	1.00
R6784:Myh1	UTSW	11	67,214,570 (GRCm38)	missense	probably damaging	0.99
R6813:Myh1	UTSW	11	67,220,460 (GRCm38)	missense	probably benign	0.34
R6866:Myh1	UTSW	11	67,224,393 (GRCm38)	missense	probably damaging	0.99
R6997:Myh1	UTSW	11	67,220,637 (GRCm38)	missense	possibly damaging	0.94
R7028:Myh1	UTSW	11	67,220,421 (GRCm38)	missense	possibly damaging	0.64
R7133:Myh1	UTSW	11	67,202,586 (GRCm38)	missense	probably benign
R7185:Myh1	UTSW	11	67,207,459 (GRCm38)	missense	probably damaging	1.00
R7194:Myh1	UTSW	11	67,211,357 (GRCm38)	missense	probably benign
R7283:Myh1	UTSW	11	67,201,844 (GRCm38)	critical splice acceptor site	probably null
R7336:Myh1	UTSW	11	67,220,609 (GRCm38)	missense	probably benign	0.00
R7348:Myh1	UTSW	11	67,202,539 (GRCm38)	missense	probably damaging	1.00
R7369:Myh1	UTSW	11	67,220,698 (GRCm38)	missense	probably damaging	1.00
R7375:Myh1	UTSW	11	67,210,428 (GRCm38)	missense	probably damaging	1.00
R7384:Myh1	UTSW	11	67,224,375 (GRCm38)	missense	possibly damaging	0.46
R7387:Myh1	UTSW	11	67,208,889 (GRCm38)	missense	probably benign	0.14
R7424:Myh1	UTSW	11	67,213,663 (GRCm38)	missense	probably damaging	1.00
R7430:Myh1	UTSW	11	67,205,567 (GRCm38)	nonsense	probably null
R7443:Myh1	UTSW	11	67,220,505 (GRCm38)	missense	probably benign
R7447:Myh1	UTSW	11	67,219,180 (GRCm38)	missense	probably benign	0.01
R7509:Myh1	UTSW	11	67,210,461 (GRCm38)	missense	probably benign	0.40
R7583:Myh1	UTSW	11	67,220,913 (GRCm38)	missense	probably benign	0.00
R7611:Myh1	UTSW	11	67,210,417 (GRCm38)	missense	possibly damaging	0.50
R7617:Myh1	UTSW	11	67,215,875 (GRCm38)	missense	possibly damaging	0.94
R7727:Myh1	UTSW	11	67,215,922 (GRCm38)	missense	probably benign	0.00
R8029:Myh1	UTSW	11	67,211,240 (GRCm38)	critical splice donor site	probably null
R8042:Myh1	UTSW	11	67,206,603 (GRCm38)	missense	probably damaging	1.00
R8060:Myh1	UTSW	11	67,215,251 (GRCm38)	missense	probably benign
R8080:Myh1	UTSW	11	67,211,402 (GRCm38)	missense	probably benign	0.10
R8117:Myh1	UTSW	11	67,222,205 (GRCm38)	missense	probably damaging	1.00
R8171:Myh1	UTSW	11	67,202,572 (GRCm38)	missense	probably damaging	1.00
R8183:Myh1	UTSW	11	67,202,006 (GRCm38)	missense	possibly damaging	0.50
R8397:Myh1	UTSW	11	67,221,639 (GRCm38)	missense	probably damaging	0.97
R8545:Myh1	UTSW	11	67,202,201 (GRCm38)	missense	probably benign	0.00
R8807:Myh1	UTSW	11	67,220,528 (GRCm38)	missense	probably benign	0.02
R8812:Myh1	UTSW	11	67,209,141 (GRCm38)	missense	probably benign	0.00
R8855:Myh1	UTSW	11	67,211,421 (GRCm38)	missense	probably damaging	1.00
R8906:Myh1	UTSW	11	67,205,913 (GRCm38)	missense	probably benign	0.02
R8959:Myh1	UTSW	11	67,211,502 (GRCm38)	missense	probably benign
R8992:Myh1	UTSW	11	67,205,781 (GRCm38)	missense	probably benign
R9140:Myh1	UTSW	11	67,209,263 (GRCm38)	missense	probably benign	0.04
R9293:Myh1	UTSW	11	67,209,103 (GRCm38)	missense	probably benign	0.25
R9366:Myh1	UTSW	11	67,219,288 (GRCm38)	missense	probably damaging	1.00
R9371:Myh1	UTSW	11	67,219,805 (GRCm38)	critical splice donor site	probably null
R9378:Myh1	UTSW	11	67,202,433 (GRCm38)	missense	probably damaging	0.99
R9482:Myh1	UTSW	11	67,217,919 (GRCm38)	missense	probably damaging	1.00
R9507:Myh1	UTSW	11	67,211,223 (GRCm38)	missense	probably benign	0.00
R9558:Myh1	UTSW	11	67,217,792 (GRCm38)	missense	possibly damaging	0.90
R9561:Myh1	UTSW	11	67,217,792 (GRCm38)	missense	possibly damaging	0.90
R9587:Myh1	UTSW	11	67,211,370 (GRCm38)	missense	probably benign	0.03
X0062:Myh1	UTSW	11	67,207,541 (GRCm38)	missense	probably damaging	0.99
Z1177:Myh1	UTSW	11	67,206,318 (GRCm38)	missense	probably damaging	1.00
Z1187:Myh1	UTSW	11	67,204,446 (GRCm38)	missense	probably benign
Z1188:Myh1	UTSW	11	67,204,446 (GRCm38)	missense	probably benign
Z1190:Myh1	UTSW	11	67,204,446 (GRCm38)	missense	probably benign
Z1191:Myh1	UTSW	11	67,204,446 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GCCTGGGACAAAAGTGCTAG -3'
(R):5'- ACGGATAGAAGCCATGGTTGTAC -3'

Sequencing Primer
(F):5'- CCTGGGACAAAAGTGCTAGTCTTG -3'
(R):5'- GAAGCCATGGTTGTACCTGCAAC -3'

Posted On

2014-09-18