Mutagenetix > Incidental Mutation

Incidental Mutation 'R3155:Vmn2r76'

263461

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r76

Ensembl Gene

ENSMUSG00000091239

Gene Name

vomeronasal 2, receptor 76

Synonyms

MMRRC Submission

040606-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.166) question?

Stock #

R3155 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85874414-85895409 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 85874959 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 673 (T673A)

Ref Sequence

ENSEMBL: ENSMUSP00000127309 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165771]

AlphaFold

E9Q3F5

Predicted Effect

probably damaging
Transcript: ENSMUST00000165771
AA Change: T673A

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000127309
Gene: ENSMUSG00000091239
AA Change: T673A

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	470	1.2e-29	PFAM
low complexity region	476	489	N/A	INTRINSIC
Pfam:NCD3G	513	565	3.7e-22	PFAM
Pfam:7tm_3	598	833	1.4e-52	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.5%

Validation Efficiency

100% (39/39)

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933407L21Rik	T	C	1: 85,859,104 (GRCm39)		probably benign	Het
Aco1	T	C	4: 40,182,915 (GRCm39)	V487A	probably damaging	Het
Adh1	A	G	3: 137,986,250 (GRCm39)	E79G	probably damaging	Het
Aebp1	A	G	11: 5,821,425 (GRCm39)	N608S	probably benign	Het
Ahctf1	G	A	1: 179,583,148 (GRCm39)	R43C	probably damaging	Het
Ahnak	A	T	19: 8,987,541 (GRCm39)	I2942L	possibly damaging	Het
Anxa9	A	T	3: 95,209,716 (GRCm39)	D134E	probably benign	Het
Ccdc14	A	G	16: 34,544,222 (GRCm39)	D860G	probably damaging	Het
Cdhr3	T	A	12: 33,099,152 (GRCm39)	I480F	possibly damaging	Het
Cldn34a	C	T	X: 151,346,840 (GRCm39)	H171Y	probably benign	Het
Cyp2d9	T	A	15: 82,336,843 (GRCm39)		probably null	Het
Dmbt1	T	A	7: 130,651,887 (GRCm39)	Y376*	probably null	Het
Fancm	T	C	12: 65,163,195 (GRCm39)	I1453T	probably benign	Het
Fbxw11	A	G	11: 32,689,244 (GRCm39)	I456V	possibly damaging	Het
Fut2	A	T	7: 45,300,091 (GRCm39)	L227Q	probably damaging	Het
Gbp5	T	C	3: 142,208,888 (GRCm39)		probably null	Het
Glrp1	C	A	1: 88,430,976 (GRCm39)	Q131H	unknown	Het
Gm6871	T	C	7: 41,223,079 (GRCm39)	N3S	probably benign	Het
H2-Eb1	C	A	17: 34,533,348 (GRCm39)	T190K	probably damaging	Het
Kdr	T	A	5: 76,129,065 (GRCm39)	I194F	probably benign	Het
Klhdc7a	A	G	4: 139,694,500 (GRCm39)	V149A	probably benign	Het
Lrit3	A	G	3: 129,585,044 (GRCm39)	F238S	probably benign	Het
Map10	A	T	8: 126,398,313 (GRCm39)	I569F	possibly damaging	Het
Myh6	A	G	14: 55,182,125 (GRCm39)	I1761T	probably damaging	Het
Npc1l1	A	C	11: 6,171,840 (GRCm39)	D874E	probably benign	Het
Or4k15c	T	C	14: 50,321,982 (GRCm39)	D52G	probably benign	Het
Or8c17	T	A	9: 38,179,836 (GRCm39)	M1K	probably null	Het
Pawr	A	G	10: 108,245,370 (GRCm39)	T193A	probably benign	Het
Ppp2r3d	T	C	9: 101,089,559 (GRCm39)	K255E	possibly damaging	Het
Rbp3	A	T	14: 33,679,071 (GRCm39)	K1006N	probably damaging	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Rin2	A	G	2: 145,702,771 (GRCm39)	K489R	probably benign	Het
Rlf	A	G	4: 121,006,529 (GRCm39)	V817A	probably damaging	Het
Rusc1	T	C	3: 88,999,038 (GRCm39)	D248G	probably benign	Het
Slc15a4	A	G	5: 127,681,600 (GRCm39)		probably null	Het
Stk4	T	A	2: 163,993,663 (GRCm39)	M98K	probably benign	Het
Taf15	T	A	11: 83,393,599 (GRCm39)	H307Q	probably benign	Het
Urgcp	A	T	11: 5,666,327 (GRCm39)	F670L	probably damaging	Het
Zfp324	G	A	7: 12,702,817 (GRCm39)	M60I	probably damaging	Het

Other mutations in Vmn2r76

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00969:Vmn2r76	APN	7	85,877,925 (GRCm39)	missense	probably benign
IGL01374:Vmn2r76	APN	7	85,874,857 (GRCm39)	missense	probably benign	0.02
IGL01419:Vmn2r76	APN	7	85,874,910 (GRCm39)	missense	probably benign	0.32
IGL01627:Vmn2r76	APN	7	85,874,871 (GRCm39)	missense	probably damaging	1.00
IGL01730:Vmn2r76	APN	7	85,879,406 (GRCm39)	missense	probably benign	0.02
IGL01957:Vmn2r76	APN	7	85,877,925 (GRCm39)	missense	probably benign
IGL02214:Vmn2r76	APN	7	85,879,138 (GRCm39)	missense	probably benign	0.07
IGL02489:Vmn2r76	APN	7	85,878,071 (GRCm39)	missense	probably benign	0.41
IGL02543:Vmn2r76	APN	7	85,879,356 (GRCm39)	missense	probably benign	0.06
IGL02579:Vmn2r76	APN	7	85,877,961 (GRCm39)	nonsense	probably null
IGL02598:Vmn2r76	APN	7	85,877,879 (GRCm39)	missense	probably benign	0.05
IGL02720:Vmn2r76	APN	7	85,874,914 (GRCm39)	missense	probably benign	0.35
IGL02745:Vmn2r76	APN	7	85,879,495 (GRCm39)	missense	probably benign	0.06
IGL03393:Vmn2r76	APN	7	85,879,034 (GRCm39)	missense	probably benign	0.01
R0483:Vmn2r76	UTSW	7	85,874,959 (GRCm39)	missense	probably damaging	1.00
R0513:Vmn2r76	UTSW	7	85,877,987 (GRCm39)	missense	probably benign	0.01
R0528:Vmn2r76	UTSW	7	85,879,506 (GRCm39)	missense	possibly damaging	0.80
R0601:Vmn2r76	UTSW	7	85,875,323 (GRCm39)	critical splice acceptor site	probably null
R0662:Vmn2r76	UTSW	7	85,879,578 (GRCm39)	missense	probably benign	0.39
R0883:Vmn2r76	UTSW	7	85,877,904 (GRCm39)	missense	probably benign	0.00
R1532:Vmn2r76	UTSW	7	85,879,454 (GRCm39)	missense	probably benign	0.02
R1694:Vmn2r76	UTSW	7	85,879,356 (GRCm39)	missense	probably benign	0.06
R1696:Vmn2r76	UTSW	7	85,880,464 (GRCm39)	missense	possibly damaging	0.56
R2135:Vmn2r76	UTSW	7	85,880,219 (GRCm39)	missense	probably benign	0.02
R2151:Vmn2r76	UTSW	7	85,879,692 (GRCm39)	missense	probably benign
R2181:Vmn2r76	UTSW	7	85,874,743 (GRCm39)	missense	probably benign	0.00
R2268:Vmn2r76	UTSW	7	85,879,707 (GRCm39)	missense	probably benign	0.03
R2877:Vmn2r76	UTSW	7	85,875,201 (GRCm39)	missense	probably benign	0.00
R3746:Vmn2r76	UTSW	7	85,874,763 (GRCm39)	missense	probably benign	0.11
R3799:Vmn2r76	UTSW	7	85,875,244 (GRCm39)	missense	probably benign	0.00
R3825:Vmn2r76	UTSW	7	85,880,415 (GRCm39)	missense	probably benign	0.10
R4058:Vmn2r76	UTSW	7	85,879,508 (GRCm39)	missense	probably benign	0.00
R4237:Vmn2r76	UTSW	7	85,879,740 (GRCm39)	missense	probably benign	0.00
R4404:Vmn2r76	UTSW	7	85,877,511 (GRCm39)	missense	probably benign	0.16
R4796:Vmn2r76	UTSW	7	85,879,652 (GRCm39)	missense	possibly damaging	0.95
R4838:Vmn2r76	UTSW	7	85,874,733 (GRCm39)	missense	probably damaging	1.00
R5175:Vmn2r76	UTSW	7	85,877,915 (GRCm39)	missense	probably benign	0.00
R5268:Vmn2r76	UTSW	7	85,875,267 (GRCm39)	missense	probably damaging	1.00
R5381:Vmn2r76	UTSW	7	85,874,496 (GRCm39)	missense	probably damaging	1.00
R5531:Vmn2r76	UTSW	7	85,874,657 (GRCm39)	missense	probably damaging	1.00
R5566:Vmn2r76	UTSW	7	85,875,286 (GRCm39)	missense	probably damaging	1.00
R5646:Vmn2r76	UTSW	7	85,875,261 (GRCm39)	missense	probably damaging	0.98
R5664:Vmn2r76	UTSW	7	85,895,202 (GRCm39)	critical splice donor site	probably null
R5818:Vmn2r76	UTSW	7	85,879,142 (GRCm39)	missense	probably benign	0.00
R6093:Vmn2r76	UTSW	7	85,877,469 (GRCm39)	nonsense	probably null
R6651:Vmn2r76	UTSW	7	85,878,059 (GRCm39)	missense	possibly damaging	0.64
R6741:Vmn2r76	UTSW	7	85,879,560 (GRCm39)	missense	probably benign
R6750:Vmn2r76	UTSW	7	85,875,114 (GRCm39)	missense	probably damaging	1.00
R7082:Vmn2r76	UTSW	7	85,874,440 (GRCm39)	missense	probably benign	0.01
R7136:Vmn2r76	UTSW	7	85,877,975 (GRCm39)	missense	probably benign	0.06
R7524:Vmn2r76	UTSW	7	85,879,374 (GRCm39)	missense	probably benign	0.00
R7524:Vmn2r76	UTSW	7	85,874,577 (GRCm39)	missense	probably benign	0.22
R7611:Vmn2r76	UTSW	7	85,879,388 (GRCm39)	missense	probably benign	0.04
R7833:Vmn2r76	UTSW	7	85,877,892 (GRCm39)	missense	probably benign
R8002:Vmn2r76	UTSW	7	85,879,271 (GRCm39)	missense	probably benign	0.05
R8021:Vmn2r76	UTSW	7	85,874,958 (GRCm39)	missense	probably damaging	1.00
R8023:Vmn2r76	UTSW	7	85,879,028 (GRCm39)	missense	probably benign	0.00
R8250:Vmn2r76	UTSW	7	85,875,231 (GRCm39)	missense	possibly damaging	0.82
R8428:Vmn2r76	UTSW	7	85,874,479 (GRCm39)	missense	possibly damaging	0.63
R8874:Vmn2r76	UTSW	7	85,877,999 (GRCm39)	missense	probably damaging	1.00
R9139:Vmn2r76	UTSW	7	85,879,170 (GRCm39)	missense	probably benign	0.02
R9357:Vmn2r76	UTSW	7	85,880,428 (GRCm39)	missense	probably benign	0.43
Z1176:Vmn2r76	UTSW	7	85,895,271 (GRCm39)	missense	probably benign	0.39

Predicted Primers

PCR Primer
(F):5'- AGGCAATAACTGAACCTTTGTTGC -3'
(R):5'- ACACTCCCATTGTCAAGGCC -3'

Sequencing Primer
(F):5'- ATGACCATGTTCAATATGCCCATC -3'
(R):5'- TTGTCAAGGCCAATAACCGAG -3'

Posted On

2015-02-05