Incidental Mutation 'R4744:Tex10'
ID 356561
Institutional Source Beutler Lab
Gene Symbol Tex10
Ensembl Gene ENSMUSG00000028345
Gene Name testis expressed gene 10
Synonyms clone 18330, 2810462N03Rik, 2610206N19Rik
MMRRC Submission 042027-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.952) question?
Stock # R4744 (G1)
Quality Score 225
Status Validated
Chromosome 4
Chromosomal Location 48430858-48473459 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 48469990 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Leucine to Serine at position 25 (L25S)
Ref Sequence ENSEMBL: ENSMUSP00000132498 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000030030] [ENSMUST00000155905] [ENSMUST00000164866]
AlphaFold Q3URQ0
Predicted Effect probably benign
Transcript: ENSMUST00000030030
AA Change: L25S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000030030
Gene: ENSMUSG00000028345
AA Change: L25S

DomainStartEndE-ValueType
low complexity region 13 25 N/A INTRINSIC
Pfam:Ipi1_N 130 235 9.7e-24 PFAM
low complexity region 832 846 N/A INTRINSIC
low complexity region 856 873 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000132911
Predicted Effect noncoding transcript
Transcript: ENSMUST00000155750
Predicted Effect probably benign
Transcript: ENSMUST00000155905
SMART Domains Protein: ENSMUSP00000114669
Gene: ENSMUSG00000028345

DomainStartEndE-ValueType
Pfam:Ipi1_N 47 152 3.4e-24 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000164866
AA Change: L25S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000132498
Gene: ENSMUSG00000028345
AA Change: L25S

DomainStartEndE-ValueType
low complexity region 13 25 N/A INTRINSIC
Pfam:Ipi1_N 132 235 4.1e-25 PFAM
low complexity region 832 846 N/A INTRINSIC
low complexity region 856 873 N/A INTRINSIC
Meta Mutation Damage Score 0.0591 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 94.3%
Validation Efficiency 99% (82/83)
MGI Phenotype PHENOTYPE: Mice homozygous for a knock-out allele exhibit embryonic lethality prior to E7.5 with impaired inner cell mass proliferation in culture. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 70 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930519G04Rik T C 5: 115,017,617 (GRCm39) S143P possibly damaging Het
4933427D14Rik T C 11: 72,066,365 (GRCm39) K614E probably damaging Het
Aatk A G 11: 119,906,948 (GRCm39) M155T possibly damaging Het
Acvr2b T C 9: 119,260,328 (GRCm39) L333P probably damaging Het
Adam22 T C 5: 8,128,699 (GRCm39) E865G probably damaging Het
Add2 A G 6: 86,087,870 (GRCm39) S358G probably damaging Het
Agap2 T C 10: 126,926,072 (GRCm39) probably null Het
Alkbh8 G A 9: 3,344,604 (GRCm39) W49* probably null Het
AU015228 A T 2: 129,942,549 (GRCm39) noncoding transcript Het
Bank1 G T 3: 135,953,450 (GRCm39) R102S probably benign Het
Brap T A 5: 121,800,193 (GRCm39) D27E probably damaging Het
Cyb5r2 G T 7: 107,349,484 (GRCm39) H276N possibly damaging Het
Dhh A G 15: 98,792,139 (GRCm39) F290L possibly damaging Het
Dhrs7 T A 12: 72,699,025 (GRCm39) N319I possibly damaging Het
Ebpl A G 14: 61,597,682 (GRCm39) V53A probably damaging Het
Eif3j2 TGCCGCCGCCGCCGCCGCCGCCGCCGCC TGCCGCCGCCGCCGCCGCCGCCGCC 18: 43,610,782 (GRCm39) probably benign Het
Etnk1 A C 6: 143,132,319 (GRCm39) N220T probably damaging Het
F11r T A 1: 171,288,166 (GRCm39) V64D probably benign Het
Fam171a1 T C 2: 3,225,946 (GRCm39) S360P probably damaging Het
Fignl1 A G 11: 11,751,585 (GRCm39) M490T probably damaging Het
Fpr-rs7 A T 17: 20,334,265 (GRCm39) M75K probably benign Het
Fzd7 T A 1: 59,523,595 (GRCm39) F493I possibly damaging Het
Galnt14 G T 17: 73,814,828 (GRCm39) P412T probably damaging Het
Gcg T A 2: 62,308,975 (GRCm39) S60C probably damaging Het
Ggt1 A G 10: 75,421,733 (GRCm39) K527E probably benign Het
Gm16551 T A 9: 74,758,153 (GRCm39) noncoding transcript Het
Gm9972 A G 11: 42,927,517 (GRCm39) K55E unknown Het
Gpr141 T A 13: 19,935,884 (GRCm39) D297V probably benign Het
Grin2b G A 6: 135,755,697 (GRCm39) S539L probably damaging Het
Hhipl1 A T 12: 108,286,238 (GRCm39) N515I possibly damaging Het
Hmcn1 T G 1: 150,453,363 (GRCm39) E5317D probably damaging Het
Hsf5 T A 11: 87,513,617 (GRCm39) N227K probably benign Het
Igfn1 A T 1: 135,910,196 (GRCm39) D129E probably benign Het
Invs T C 4: 48,397,609 (GRCm39) F339L probably damaging Het
Jak2 T A 19: 29,239,656 (GRCm39) S17T probably benign Het
Mdga2 T A 12: 66,844,501 (GRCm39) I166F probably benign Het
Nck1 T C 9: 100,388,797 (GRCm39) I6V probably benign Het
Neb T C 2: 52,040,589 (GRCm39) D6624G probably benign Het
Nmur2 A G 11: 55,931,661 (GRCm39) Y17H probably benign Het
Nwd2 T C 5: 63,964,310 (GRCm39) L1298P probably damaging Het
Ocel1 A G 8: 71,825,397 (GRCm39) E161G probably damaging Het
Or1j16 A T 2: 36,530,991 (GRCm39) probably null Het
Pabpc2 A G 18: 39,907,881 (GRCm39) Y382C probably benign Het
Panx1 A G 9: 14,921,594 (GRCm39) probably benign Het
Pdhx A T 2: 102,872,641 (GRCm39) V147D probably benign Het
Pigz A T 16: 31,764,151 (GRCm39) H403L probably damaging Het
Pilra T C 5: 137,833,769 (GRCm39) probably null Het
Rbm47 T C 5: 66,184,036 (GRCm39) D189G probably damaging Het
Rhobtb2 A G 14: 70,031,451 (GRCm39) L558P probably damaging Het
Scarf2 G A 16: 17,621,380 (GRCm39) R322H probably damaging Het
Septin3 T C 15: 82,174,658 (GRCm39) probably null Het
Sirt2 T C 7: 28,476,438 (GRCm39) F26L probably damaging Het
Slc1a1 A T 19: 28,871,925 (GRCm39) T133S probably benign Het
Slc1a2 A G 2: 102,568,214 (GRCm39) I84V probably benign Het
Slc28a2b A T 2: 122,353,286 (GRCm39) K489* probably null Het
Slc6a9 A T 4: 117,725,092 (GRCm39) Q562L probably benign Het
Snx29 C T 16: 11,167,773 (GRCm39) Q25* probably null Het
St6galnac6 A G 2: 32,508,555 (GRCm39) I231V probably damaging Het
Stard9 A G 2: 120,526,604 (GRCm39) T954A probably benign Het
Sufu A G 19: 46,472,069 (GRCm39) M443V possibly damaging Het
Sv2b T C 7: 74,856,266 (GRCm39) D8G probably benign Het
Tapbpl G A 6: 125,205,248 (GRCm39) R233W probably damaging Het
Trp53bp1 A T 2: 121,041,794 (GRCm39) V1254D probably damaging Het
Ugt3a1 T A 15: 9,310,639 (GRCm39) I307N probably benign Het
Unc13c T C 9: 73,839,126 (GRCm39) D575G probably damaging Het
Usf2 A T 7: 30,654,197 (GRCm39) D166E probably damaging Het
Usp25 T A 16: 76,911,877 (GRCm39) L969M probably damaging Het
Usp32 G A 11: 84,885,219 (GRCm39) P1276L probably damaging Het
Vmn2r8 T A 5: 108,956,447 (GRCm39) E58D probably benign Het
Zfp462 G T 4: 55,011,598 (GRCm39) C40F probably damaging Het
Other mutations in Tex10
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00574:Tex10 APN 4 48,469,937 (GRCm39) nonsense probably null
IGL00832:Tex10 APN 4 48,468,864 (GRCm39) missense probably benign
IGL01376:Tex10 APN 4 48,456,740 (GRCm39) missense possibly damaging 0.90
IGL01594:Tex10 APN 4 48,469,906 (GRCm39) missense possibly damaging 0.47
IGL02754:Tex10 APN 4 48,435,028 (GRCm39) missense possibly damaging 0.46
IGL03071:Tex10 APN 4 48,452,946 (GRCm39) missense probably benign 0.00
IGL03399:Tex10 APN 4 48,459,915 (GRCm39) missense probably benign 0.04
R0105:Tex10 UTSW 4 48,468,957 (GRCm39) missense probably damaging 0.99
R0105:Tex10 UTSW 4 48,468,957 (GRCm39) missense probably damaging 0.99
R0544:Tex10 UTSW 4 48,462,766 (GRCm39) splice site probably null
R0583:Tex10 UTSW 4 48,451,952 (GRCm39) missense probably damaging 1.00
R0591:Tex10 UTSW 4 48,456,800 (GRCm39) missense probably benign 0.04
R0592:Tex10 UTSW 4 48,456,800 (GRCm39) missense probably benign 0.04
R0593:Tex10 UTSW 4 48,456,800 (GRCm39) missense probably benign 0.04
R0893:Tex10 UTSW 4 48,456,800 (GRCm39) missense probably benign 0.04
R1485:Tex10 UTSW 4 48,436,492 (GRCm39) missense possibly damaging 0.54
R1703:Tex10 UTSW 4 48,456,800 (GRCm39) missense probably benign 0.04
R1704:Tex10 UTSW 4 48,456,800 (GRCm39) missense probably benign 0.04
R1706:Tex10 UTSW 4 48,456,800 (GRCm39) missense probably benign 0.04
R1911:Tex10 UTSW 4 48,456,800 (GRCm39) missense probably benign 0.04
R1912:Tex10 UTSW 4 48,456,800 (GRCm39) missense probably benign 0.04
R1930:Tex10 UTSW 4 48,456,800 (GRCm39) missense probably benign 0.04
R1983:Tex10 UTSW 4 48,460,059 (GRCm39) missense possibly damaging 0.93
R2001:Tex10 UTSW 4 48,451,940 (GRCm39) missense probably damaging 1.00
R2074:Tex10 UTSW 4 48,456,800 (GRCm39) missense probably benign 0.04
R2075:Tex10 UTSW 4 48,456,800 (GRCm39) missense probably benign 0.04
R2157:Tex10 UTSW 4 48,436,522 (GRCm39) splice site probably benign
R3000:Tex10 UTSW 4 48,459,393 (GRCm39) splice site probably null
R4067:Tex10 UTSW 4 48,459,355 (GRCm39) nonsense probably null
R4081:Tex10 UTSW 4 48,468,873 (GRCm39) missense probably benign 0.11
R4133:Tex10 UTSW 4 48,468,968 (GRCm39) missense probably damaging 1.00
R4352:Tex10 UTSW 4 48,452,039 (GRCm39) missense possibly damaging 0.77
R4364:Tex10 UTSW 4 48,468,774 (GRCm39) missense probably benign 0.13
R4601:Tex10 UTSW 4 48,452,946 (GRCm39) missense probably benign 0.00
R4602:Tex10 UTSW 4 48,452,946 (GRCm39) missense probably benign 0.00
R4610:Tex10 UTSW 4 48,452,946 (GRCm39) missense probably benign 0.00
R4707:Tex10 UTSW 4 48,468,984 (GRCm39) missense probably benign 0.00
R4778:Tex10 UTSW 4 48,436,468 (GRCm39) missense probably damaging 1.00
R4989:Tex10 UTSW 4 48,458,525 (GRCm39) splice site probably benign
R5051:Tex10 UTSW 4 48,460,019 (GRCm39) missense possibly damaging 0.86
R5120:Tex10 UTSW 4 48,459,272 (GRCm39) missense possibly damaging 0.68
R5732:Tex10 UTSW 4 48,460,046 (GRCm39) missense probably damaging 1.00
R5799:Tex10 UTSW 4 48,433,295 (GRCm39) missense possibly damaging 0.62
R5813:Tex10 UTSW 4 48,452,928 (GRCm39) missense probably benign 0.00
R6091:Tex10 UTSW 4 48,459,891 (GRCm39) missense probably damaging 0.98
R6223:Tex10 UTSW 4 48,468,525 (GRCm39) missense probably damaging 0.98
R6493:Tex10 UTSW 4 48,436,450 (GRCm39) missense probably damaging 1.00
R7567:Tex10 UTSW 4 48,468,787 (GRCm39) missense possibly damaging 0.93
R7590:Tex10 UTSW 4 48,467,725 (GRCm39) missense probably damaging 0.99
R7808:Tex10 UTSW 4 48,459,984 (GRCm39) missense probably benign
R8004:Tex10 UTSW 4 48,452,047 (GRCm39) missense possibly damaging 0.64
R8084:Tex10 UTSW 4 48,431,066 (GRCm39) missense probably benign 0.05
R9030:Tex10 UTSW 4 48,452,056 (GRCm39) missense probably damaging 1.00
X0017:Tex10 UTSW 4 48,460,080 (GRCm39) missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- TGTATAACAATACACCATATGGCCC -3'
(R):5'- CGGCATTGTTATTGATGGCA -3'

Sequencing Primer
(F):5'- AATACACCATATGGCCCTAAGG -3'
(R):5'- CGGCATTGTTATTGATGGCATGATTC -3'
Posted On 2015-11-11