Mutagenetix > Incidental Mutations

Incidental Mutation 'R4861:Tas2r117'

380248

Institutional Source

Beutler Lab

Gene Symbol

Tas2r117

Ensembl Gene

ENSMUSG00000058349

Gene Name

taste receptor, type 2, member 117

Synonyms

Tas2r17, T2R17, mt2r54, mGR17

MMRRC Submission

042472-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R4861 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

132802818-132803975 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 132803129 bp

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 77 (F77L)

Ref Sequence

ENSEMBL: ENSMUSP00000069768 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068302]

Predicted Effect

probably benign
Transcript: ENSMUST00000068302
AA Change: F77L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000069768
Gene: ENSMUSG00000058349
AA Change: F77L

Domain	Start	End	E-Value	Type
Pfam:TAS2R	8	307	1.2e-85	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 98.8%
3x: 97.8%
10x: 94.2%
20x: 84.7%

Validation Efficiency

100% (53/53)

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb11	C	T	2: 69,245,905	R1153H	probably damaging	Het
Ahcy	T	C	2: 155,060,516	E411G	probably benign	Het
Alpi	T	A	1: 87,100,469	I211F	probably damaging	Het
Arfgef3	G	A	10: 18,607,731	A1415V	probably benign	Het
Car3	T	C	3: 14,866,896	V109A	probably damaging	Het
Ccdc114	A	G	7: 45,942,873	E359G	probably damaging	Het
Cdk13	A	T	13: 17,766,586	V17D	probably damaging	Het
Cept1	A	C	3: 106,505,732	S226A	probably damaging	Het
Dbt	A	T	3: 116,548,078	I443L	probably benign	Het
Dync1h1	C	A	12: 110,658,126	T3700N	probably damaging	Het
Farp2	T	C	1: 93,605,419	L633S	probably damaging	Het
Frmd6	T	G	12: 70,893,726	S382A	probably damaging	Het
Gm26727	T	C	2: 67,432,945	I79M	probably damaging	Het
Gm5800	T	A	14: 51,716,047	N37I	probably damaging	Het
Hapln1	G	A	13: 89,601,452	G39S	possibly damaging	Het
Ice2	T	A	9: 69,415,448	S408R	probably benign	Het
Lctl	T	C	9: 64,119,763	I131T	possibly damaging	Het
Mvk	C	T	5: 114,460,197		probably benign	Het
Mzt1	T	C	14: 99,047,474		probably null	Het
Ncoa7	T	A	10: 30,704,612	M117L	probably benign	Het
Npy4r	C	T	14: 34,146,883	W149*	probably null	Het
Nr5a2	A	G	1: 136,948,720		probably null	Het
Plg	G	A	17: 12,395,735	E301K	probably benign	Het
Pnkp	C	T	7: 44,862,403	S113L	probably damaging	Het
Rapgef2	T	C	3: 79,074,436	K1084R	probably benign	Het
Slc41a2	T	C	10: 83,316,458	Q51R	probably damaging	Het
Slc47a2	A	T	11: 61,336,233	C170S	probably benign	Het
Slco1b2	A	T	6: 141,671,222	N427I	possibly damaging	Het
Smc2	G	A	4: 52,461,090	R571H	probably benign	Het
Sp4	G	T	12: 118,300,811		probably null	Het
Tbcd	C	T	11: 121,601,961	R875C	probably damaging	Het
Thumpd2	A	G	17: 81,026,801	S453P	probably benign	Het
Vars2	G	T	17: 35,661,933	Q13K	probably benign	Het

Other mutations in Tas2r117

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01611:Tas2r117	APN	6	132803484	missense	probably benign	0.00
IGL01611:Tas2r117	APN	6	132803487	missense	probably damaging	0.96
IGL02140:Tas2r117	APN	6	132803595	missense	probably benign	0.15
IGL02154:Tas2r117	APN	6	132803715	missense	probably benign	0.00
IGL02466:Tas2r117	APN	6	132803000	missense	probably benign	0.12
IGL02942:Tas2r117	APN	6	132803694	missense	probably benign	0.00
IGL03328:Tas2r117	APN	6	132803078	missense	probably benign	0.40
PIT4480001:Tas2r117	UTSW	6	132803051	missense	possibly damaging	0.91
R0380:Tas2r117	UTSW	6	132803588	nonsense	probably null
R0456:Tas2r117	UTSW	6	132803391	missense	probably benign	0.12
R0699:Tas2r117	UTSW	6	132803198	missense	probably damaging	1.00
R2118:Tas2r117	UTSW	6	132803166	missense	probably damaging	0.96
R2265:Tas2r117	UTSW	6	132803225	missense	probably benign	0.06
R4420:Tas2r117	UTSW	6	132803349	nonsense	probably null
R4861:Tas2r117	UTSW	6	132803129	missense	probably benign	0.00
R5233:Tas2r117	UTSW	6	132803622	missense	possibly damaging	0.95
R5384:Tas2r117	UTSW	6	132803154	missense	probably benign	0.04
R6750:Tas2r117	UTSW	6	132802854	start gained	probably benign
R6852:Tas2r117	UTSW	6	132802929	missense	probably benign	0.00
R6902:Tas2r117	UTSW	6	132803325	missense	probably damaging	0.98
R6946:Tas2r117	UTSW	6	132803325	missense	probably damaging	0.98
R7129:Tas2r117	UTSW	6	132803387	missense	probably benign	0.01
R7412:Tas2r117	UTSW	6	132803229	missense	probably damaging	1.00
R7733:Tas2r117	UTSW	6	132803175	missense	probably benign	0.02
R7768:Tas2r117	UTSW	6	132803522	missense	probably damaging	1.00
R7953:Tas2r117	UTSW	6	132803318	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTCCCAGAGCACACTTTCAG -3'
(R):5'- TCAGGAGGAGCAATGACACC -3'

Sequencing Primer
(F):5'- GCACACTTTCAGTCATTTTAATCGTG -3'
(R):5'- AACTGAAACTACTTTTTCAACTCTCC -3'

Posted On

2016-04-15