Mutagenetix > Incidental Mutation

Incidental Mutation 'R7455:Oprm1'

578082

Institutional Source

Beutler Lab

Gene Symbol

Oprm1

Ensembl Gene

ENSMUSG00000000766

Gene Name

opioid receptor, mu 1

Synonyms

MOP-R, MOR-1, MOP receptor, mor, Oprm, muOR

MMRRC Submission

045529-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.110) question?

Stock #

R7455 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

6708593-6988209 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 6780204 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 289 (F289L)

Ref Sequence

ENSEMBL: ENSMUSP00000077704 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000000783] [ENSMUST00000052751] [ENSMUST00000056385] [ENSMUST00000063036] [ENSMUST00000078634] [ENSMUST00000092729] [ENSMUST00000092731] [ENSMUST00000092734] [ENSMUST00000105597] [ENSMUST00000105601] [ENSMUST00000105602] [ENSMUST00000105603] [ENSMUST00000105604] [ENSMUST00000105605] [ENSMUST00000105607] [ENSMUST00000105611] [ENSMUST00000105615] [ENSMUST00000123861] [ENSMUST00000129221] [ENSMUST00000129954] [ENSMUST00000135502] [ENSMUST00000143875] [ENSMUST00000144264] [ENSMUST00000147171] [ENSMUST00000150374] [ENSMUST00000152674] [ENSMUST00000154906] [ENSMUST00000154941]

AlphaFold

P42866

Predicted Effect

probably damaging
Transcript: ENSMUST00000000783
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000000783
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	339	1.6e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.3e-10	PFAM
Pfam:7tm_1	85	336	4e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000052751
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000060329
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.1e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.2e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000056385
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000060590
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.3e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000063036
AA Change: F221L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000053498
Gene: ENSMUSG00000000766
AA Change: F221L

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	268	8.7e-64	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000078634
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000077704
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	339	2.1e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	2.4e-10	PFAM
Pfam:7tm_1	85	336	9e-60	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000092729
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000090405
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.6e-11	PFAM
Pfam:7tm_1	85	336	3.1e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000092731
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000090407
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.9e-11	PFAM
Pfam:7tm_1	85	336	3.2e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000092734
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000090410
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.3e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105597

Predicted Effect

probably benign
Transcript: ENSMUST00000105601
AA Change: I171T

PolyPhen 2 Score 0.400 (Sensitivity: 0.89; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000101226
Gene: ENSMUSG00000000766
AA Change: I171T

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	46	100	3e-9	SMART
PDB:4DKL\|A	52	100	3e-21	PDB
low complexity region	119	131	N/A	INTRINSIC
low complexity region	173	184	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000105602
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000101227
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.8e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.8e-11	PFAM
Pfam:7tm_1	85	336	3.1e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000105603
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000101228
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	6.7e-8	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.6e-11	PFAM
Pfam:7tm_1	85	336	3.6e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000105604
AA Change: F289L

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000101229
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	332	5.1e-8	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.9e-11	PFAM
Pfam:7tm_1	85	336	3.8e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000105605
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000101230
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.8e-11	PFAM
Pfam:7tm_1	85	336	3.2e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000105607
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000101232
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.3e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000105611
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000101236
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	338	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.4e-10	PFAM
Pfam:7tm_1	85	336	4.4e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000105615
AA Change: F221L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000101240
Gene: ENSMUSG00000000766
AA Change: F221L

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	268	1.3e-63	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000123861
AA Change: I171T

PolyPhen 2 Score 0.400 (Sensitivity: 0.89; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000120187
Gene: ENSMUSG00000000766
AA Change: I171T

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	46	100	3e-9	SMART
PDB:4DKL\|A	52	100	3e-21	PDB
low complexity region	119	131	N/A	INTRINSIC
low complexity region	173	184	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000129221
AA Change: F225L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000123117
Gene: ENSMUSG00000000766
AA Change: F225L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	12	261	1.1e-7	PFAM
Pfam:7TM_GPCR_Srsx	15	287	7.3e-11	PFAM
Pfam:7tm_1	21	272	2.4e-67	PFAM
low complexity region	340	351	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000129954
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000122385
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	338	6.9e-8	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.5e-10	PFAM
Pfam:7tm_1	85	336	5.4e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000135502
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000135143
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	339	2.2e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.9e-10	PFAM
Pfam:7tm_1	85	336	7.5e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000143875

Predicted Effect

probably damaging
Transcript: ENSMUST00000144264
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000115836
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.4e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000147171
AA Change: F221L

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000117950
Gene: ENSMUSG00000000766
AA Change: F221L

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	268	9.2e-64	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000150374

Predicted Effect

probably benign
Transcript: ENSMUST00000152674

SMART Domains

Protein: ENSMUSP00000115552
Gene: ENSMUSG00000000766

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	46	94	8e-8	SMART
PDB:4DKL\|A	52	94	7e-23	PDB

Predicted Effect

probably damaging
Transcript: ENSMUST00000154906
AA Change: F289L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000114342
Gene: ENSMUSG00000000766
AA Change: F289L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	332	1.5e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.1e-10	PFAM
Pfam:7tm_1	85	336	3.6e-67	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000154941
AA Change: F225L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000115413
Gene: ENSMUSG00000000766
AA Change: F225L

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	12	261	9.6e-8	PFAM
Pfam:7TM_GPCR_Srsx	15	287	6.1e-11	PFAM
Pfam:7tm_1	21	272	2e-67	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

98% (62/63)

MGI Phenotype

FUNCTION: This gene encodes the mu opioid receptor which is where drugs such as morphine and other opioids have pharmacological effects. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
PHENOTYPE: Homozygotes for null mutations exhibit isoform dependent loss of behavioral and gastrointestinal opioid responses and may also show impaired spatial memory, heightened nociception, reduced locomotor activity, increased hematopoietic proliferation, and decreased male fertility. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agbl1	C	T	7: 76,074,503 (GRCm39)	H646Y	unknown	Het
Ak5	C	T	3: 152,187,209 (GRCm39)	R426H	probably damaging	Het
Akap9	T	C	5: 4,022,792 (GRCm39)	V1207A	probably benign	Het
Asph	A	T	4: 9,531,732 (GRCm39)		probably null	Het
Avpr1a	T	G	10: 122,285,169 (GRCm39)	C154G	probably damaging	Het
Boll	T	C	1: 55,339,262 (GRCm39)	Q283R	probably benign	Het
Cad	C	T	5: 31,231,506 (GRCm39)	P1743S	probably damaging	Het
Ccdc137	T	G	11: 120,350,985 (GRCm39)	M129R	probably damaging	Het
Cdh23	A	G	10: 60,142,003 (GRCm39)	I3028T	possibly damaging	Het
Cep68	T	A	11: 20,180,571 (GRCm39)	I687F	probably damaging	Het
Ces2a	T	A	8: 105,464,154 (GRCm39)	I262N	probably damaging	Het
Cfap44	A	G	16: 44,225,147 (GRCm39)		probably benign	Het
Cfap69	T	G	5: 5,675,873 (GRCm39)	H247P	possibly damaging	Het
Cideb	T	C	14: 55,992,292 (GRCm39)	T134A	probably damaging	Het
Cilp2	A	T	8: 70,333,721 (GRCm39)	D1092E	probably damaging	Het
Cptp	G	A	4: 155,950,957 (GRCm39)	R170C	probably damaging	Het
Cysrt1	A	G	2: 25,129,422 (GRCm39)	L30S	probably benign	Het
Dcc	A	G	18: 71,553,394 (GRCm39)	V754A	probably benign	Het
Entrep2	A	G	7: 64,409,161 (GRCm39)	V411A	probably benign	Het
Fer1l5	A	T	1: 36,428,064 (GRCm39)	T453S	probably benign	Het
Fnbp4	ACCACCTCCACCTCCACCTCC	ACCACCTCCACCTCCACCTCCACCTCC	2: 90,608,159 (GRCm39)		probably benign	Het
Frem2	C	T	3: 53,479,701 (GRCm39)		probably null	Het
Gm13941	A	G	2: 110,925,085 (GRCm39)	F173L	unknown	Het
Greb1l	G	A	18: 10,554,915 (GRCm39)	A1654T	probably damaging	Het
Inpp4b	T	A	8: 82,798,332 (GRCm39)	I840N	probably damaging	Het
Kdm4b	T	A	17: 56,703,657 (GRCm39)	I746N	probably damaging	Het
Lins1	C	A	7: 66,361,692 (GRCm39)	H448N	probably benign	Het
Ly9	A	T	1: 171,421,507 (GRCm39)	Y581*	probably null	Het
Mark1	T	A	1: 184,651,947 (GRCm39)	E186V	probably damaging	Het
Mcph1	T	A	8: 18,681,775 (GRCm39)	V304E	probably benign	Het
Mical3	T	C	6: 120,935,705 (GRCm39)	D1607G	probably damaging	Het
Mup18	A	G	4: 61,592,171 (GRCm39)	V31A	probably benign	Het
Nectin3	A	T	16: 46,317,105 (GRCm39)	C11*	probably null	Het
Nedd1	A	T	10: 92,536,787 (GRCm39)	L172M	probably benign	Het
Nim1k	G	A	13: 120,173,995 (GRCm39)	R300W	probably damaging	Het
Or4c115	G	T	2: 88,927,434 (GRCm39)	P279Q	probably damaging	Het
Or7g12	A	G	9: 18,900,150 (GRCm39)	I289V	possibly damaging	Het
Or8c8	T	C	9: 38,165,550 (GRCm39)	V276A	probably damaging	Het
Parg	T	C	14: 31,931,432 (GRCm39)	Y351H	probably benign	Het
Phf3	A	C	1: 30,876,239 (GRCm39)	V118G	probably damaging	Het
Pik3c2g	T	C	6: 139,913,643 (GRCm39)	Y1014H	unknown	Het
Pmp22	A	G	11: 63,025,339 (GRCm39)		probably null	Het
Ppfibp1	T	A	6: 146,917,848 (GRCm39)	F501L	probably damaging	Het
Rgl2	G	A	17: 34,151,657 (GRCm39)	A234T	probably benign	Het
Rhpn2	G	A	7: 35,070,669 (GRCm39)		probably null	Het
Ryr3	T	C	2: 112,559,211 (GRCm39)	K3058E	probably damaging	Het
Sbsn	A	T	7: 30,452,602 (GRCm39)	Q539L	possibly damaging	Het
Scgn	G	A	13: 24,150,848 (GRCm39)	R168C	probably benign	Het
Serpinb10	A	G	1: 107,463,832 (GRCm39)	Y38C	probably damaging	Het
Sfmbt2	G	A	2: 10,582,766 (GRCm39)	V709I	probably benign	Het
Slc1a2	A	T	2: 102,566,299 (GRCm39)	M10L	probably benign	Het
Sox6	T	G	7: 115,088,904 (GRCm39)	I653L	probably benign	Het
Tespa1	A	G	10: 130,196,559 (GRCm39)	I166V	probably benign	Het
Tex15	T	C	8: 34,067,025 (GRCm39)	Y2152H	possibly damaging	Het
Tlr9	A	G	9: 106,101,729 (GRCm39)	N340S	probably benign	Het
Tmem198	G	A	1: 75,456,430 (GRCm39)	R28Q	unknown	Het
Tut7	T	C	13: 59,969,871 (GRCm39)	Y8C	probably benign	Het
Vmn2r7	T	C	3: 64,624,014 (GRCm39)	D102G	probably benign	Het
Wdr93	A	G	7: 79,425,267 (GRCm39)	M529V	probably benign	Het
Zfp110	A	G	7: 12,581,984 (GRCm39)	H310R	probably benign	Het
Zfp36l2	A	G	17: 84,494,575 (GRCm39)	S21P	probably damaging	Het
Zfp534	G	A	4: 147,759,212 (GRCm39)	H486Y	probably damaging	Het
Zfp638	G	A	6: 83,907,127 (GRCm39)	V431I	probably damaging	Het

Other mutations in Oprm1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01018:Oprm1	APN	10	6,987,170 (GRCm39)	utr 3 prime	probably benign
IGL01768:Oprm1	APN	10	6,779,186 (GRCm39)	missense	probably damaging	1.00
IGL02455:Oprm1	APN	10	6,780,219 (GRCm39)	missense	probably damaging	1.00
IGL03391:Oprm1	APN	10	6,964,077 (GRCm39)	intron	probably benign
IGL03410:Oprm1	APN	10	6,780,051 (GRCm39)	missense	probably damaging	1.00
IGL03048:Oprm1	UTSW	10	6,779,064 (GRCm39)	missense	probably damaging	1.00
R0189:Oprm1	UTSW	10	6,739,071 (GRCm39)	missense	possibly damaging	0.94
R0321:Oprm1	UTSW	10	6,779,183 (GRCm39)	missense	probably damaging	1.00
R0629:Oprm1	UTSW	10	6,782,604 (GRCm39)	splice site	probably null
R0730:Oprm1	UTSW	10	6,782,652 (GRCm39)	intron	probably benign
R1542:Oprm1	UTSW	10	6,738,960 (GRCm39)	missense	probably damaging	1.00
R1743:Oprm1	UTSW	10	6,780,105 (GRCm39)	missense	probably damaging	0.99
R1874:Oprm1	UTSW	10	6,739,035 (GRCm39)	missense	probably benign	0.17
R2864:Oprm1	UTSW	10	6,744,226 (GRCm39)	splice site	probably null
R2964:Oprm1	UTSW	10	6,738,914 (GRCm39)	missense	probably damaging	0.98
R3792:Oprm1	UTSW	10	6,789,544 (GRCm39)	missense	probably benign	0.00
R4008:Oprm1	UTSW	10	6,782,520 (GRCm39)	missense	probably benign
R4049:Oprm1	UTSW	10	6,779,087 (GRCm39)	missense	probably benign	0.36
R4088:Oprm1	UTSW	10	6,780,234 (GRCm39)	missense	probably damaging	1.00
R4724:Oprm1	UTSW	10	6,708,656 (GRCm39)	nonsense	probably null
R4812:Oprm1	UTSW	10	6,782,698 (GRCm39)	intron	probably benign
R4822:Oprm1	UTSW	10	6,779,036 (GRCm39)	missense	probably damaging	0.99
R4855:Oprm1	UTSW	10	6,788,468 (GRCm39)	missense	probably benign	0.01
R5072:Oprm1	UTSW	10	6,782,550 (GRCm39)	missense	probably benign	0.15
R5768:Oprm1	UTSW	10	6,739,026 (GRCm39)	missense	probably damaging	1.00
R5770:Oprm1	UTSW	10	6,739,026 (GRCm39)	missense	probably damaging	1.00
R5995:Oprm1	UTSW	10	6,782,520 (GRCm39)	missense	probably benign
R6327:Oprm1	UTSW	10	6,780,063 (GRCm39)	missense	probably damaging	0.99
R7135:Oprm1	UTSW	10	6,780,203 (GRCm39)	missense	possibly damaging	0.77
R7413:Oprm1	UTSW	10	6,778,919 (GRCm39)	missense	probably damaging	1.00
R8192:Oprm1	UTSW	10	6,788,417 (GRCm39)	missense	probably benign	0.04
R8210:Oprm1	UTSW	10	6,780,442 (GRCm39)	missense	probably benign	0.02
R8945:Oprm1	UTSW	10	6,782,644 (GRCm39)	intron	probably benign
R9054:Oprm1	UTSW	10	6,773,914 (GRCm39)	intron	probably benign
R9723:Oprm1	UTSW	10	6,788,514 (GRCm39)	missense	possibly damaging	0.66
R9726:Oprm1	UTSW	10	6,929,694 (GRCm39)	missense	probably benign	0.00
X0066:Oprm1	UTSW	10	6,780,462 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TCTCATCCCACATGGTACTGG -3'
(R):5'- AAGTTGGGATGCAGAACTCTC -3'

Sequencing Primer
(F):5'- GGAGAACCTGCTCAAAATCTGTGTC -3'
(R):5'- GTTGGGATGCAGAACTCTCTAAAAC -3'

Posted On

2019-10-07