Incidental Mutation 'R7710:Lrrn4'
ID594515
Institutional Source Beutler Lab
Gene Symbol Lrrn4
Ensembl Gene ENSMUSG00000043110
Gene Nameleucine rich repeat neuronal 4
SynonymsB430119L13Rik
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.080) question?
Stock #R7710 (G1)
Quality Score209.009
Status Not validated
Chromosome2
Chromosomal Location132868305-132880891 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 132879531 bp
ZygosityHeterozygous
Amino Acid Change Histidine to Arginine at position 122 (H122R)
Ref Sequence ENSEMBL: ENSMUSP00000057005 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000049787]
Predicted Effect probably benign
Transcript: ENSMUST00000049787
AA Change: H122R

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000057005
Gene: ENSMUSG00000043110
AA Change: H122R

DomainStartEndE-ValueType
signal peptide 1 21 N/A INTRINSIC
LRR 105 126 7.36e0 SMART
LRR_TYP 128 151 3.44e-4 SMART
LRR 153 175 1.19e1 SMART
LRR 176 199 1.53e1 SMART
LRR 205 228 2.03e1 SMART
LRR 229 253 3.36e2 SMART
LRRCT 311 363 6.92e-2 SMART
low complexity region 520 530 N/A INTRINSIC
FN3 577 656 3.73e-10 SMART
transmembrane domain 679 701 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.2%
Validation Efficiency
MGI Phenotype PHENOTYPE: Homozygous null mutant mice exhibit impaired memory retention in hippocampus- dependent learning tasks such as contextual conditioning and spatial learning. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 65 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adamtsl1 G A 4: 86,232,573 E151K Het
Ap3b1 T C 13: 94,451,073 S452P probably damaging Het
Cacna2d4 A G 6: 119,274,239 I463V probably benign Het
Ccdc142 T C 6: 83,101,696 S5P probably benign Het
Cd300ld C T 11: 114,984,212 V199M probably damaging Het
Cep162 T C 9: 87,232,119 Y300C probably damaging Het
Ces2h C T 8: 105,000,865 Q5* probably null Het
Col9a3 C T 2: 180,609,365 L310F probably damaging Het
Copa A G 1: 172,109,844 D454G possibly damaging Het
Ctnnbip1 A C 4: 149,545,820 M29L probably benign Het
Ctnnbl1 A G 2: 157,774,571 D64G probably benign Het
Dcxr T A 11: 120,727,082 T23S probably benign Het
Dennd1b T A 1: 139,062,932 H211Q probably damaging Het
Ercc8 G A 13: 108,183,863 A328T probably benign Het
Fam217a T C 13: 34,911,128 D216G possibly damaging Het
Fam26d A T 10: 34,044,049 M74K possibly damaging Het
Fat2 T A 11: 55,310,763 Y495F probably benign Het
Fbxw13 G A 9: 109,195,900 S15F probably damaging Het
Fras1 T A 5: 96,645,103 C964* probably null Het
Fshr T A 17: 88,985,255 H665L probably benign Het
Gm10447 A T 11: 53,456,610 C31S unknown Het
Gm13272 A T 4: 88,780,349 Q167L probably benign Het
Gtpbp2 T C 17: 46,167,787 I510T possibly damaging Het
Hps6 G A 19: 46,004,568 A315T probably benign Het
Igfbp6 A C 15: 102,147,850 T115P probably damaging Het
Ikzf4 A G 10: 128,632,741 V623A possibly damaging Het
Il11ra1 T C 4: 41,764,846 V100A probably benign Het
Ints1 T C 5: 139,771,085 T493A probably benign Het
Itgax C T 7: 128,135,856 T453I probably benign Het
Kras T C 6: 145,220,628 T183A probably benign Het
Lhcgr T C 17: 88,742,782 T439A probably damaging Het
Muc2 A T 7: 141,700,883 R210S possibly damaging Het
Myh7 C G 14: 54,988,801 D461H probably damaging Het
Myo18b T C 5: 112,875,025 D167G unknown Het
Nedd8 G A 14: 55,671,989 probably benign Het
Olfr1442 A C 19: 12,674,976 Y257S probably damaging Het
Pars2 T A 4: 106,654,079 Y353N probably damaging Het
Pbxip1 T A 3: 89,448,101 D642E probably damaging Het
Pcdhb10 A T 18: 37,413,601 R577* probably null Het
Pcna-ps2 T A 19: 9,284,125 Y249* probably null Het
Peg10 GC GCTCC 6: 4,756,452 probably benign Het
Pla2g6 A T 15: 79,287,158 V744E probably damaging Het
Ppfibp1 A G 6: 146,996,405 I207V probably benign Het
Prr11 T C 11: 87,103,607 D71G probably benign Het
Rnf150 A C 8: 82,864,152 Y48S probably damaging Het
Rsf1 G A 7: 97,681,834 G1237D Het
Rusc2 C T 4: 43,416,119 T475I probably benign Het
Scgb1b20 T A 7: 33,373,442 I25N probably damaging Het
Sdr39u1 T C 14: 55,899,659 N65S probably benign Het
Sh3gl3 T C 7: 82,284,086 V219A possibly damaging Het
Shcbp1 A G 8: 4,764,965 F171S probably benign Het
Shq1 A G 6: 100,671,045 F6S probably damaging Het
Slc12a4 C T 8: 105,945,571 R868H possibly damaging Het
Smg6 T C 11: 74,930,619 V572A probably benign Het
Syn3 A G 10: 86,407,670 V135A probably damaging Het
Tarsl2 A T 7: 65,664,969 D425V probably benign Het
Tmem253 A T 14: 52,017,151 Q21L possibly damaging Het
Trpm3 G T 19: 22,918,790 R997L probably damaging Het
Ubr5 G A 15: 37,979,832 A2434V probably null Het
Ugt1a9 A G 1: 88,071,109 T94A probably benign Het
Vmn1r62 A G 7: 5,675,183 probably benign Het
Vmn2r61 T C 7: 42,267,048 C362R probably damaging Het
Zfp354c TCACACTCGGCACA TCACA 11: 50,815,240 probably benign Het
Zfp94 T C 7: 24,303,682 K112E probably benign Het
Zfp954 A T 7: 7,117,890 V47E probably damaging Het
Other mutations in Lrrn4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00333:Lrrn4 APN 2 132870817 missense probably damaging 1.00
IGL00661:Lrrn4 APN 2 132870668 missense probably benign 0.08
IGL01542:Lrrn4 APN 2 132879472 missense probably benign
IGL01584:Lrrn4 APN 2 132878076 missense probably damaging 1.00
IGL01723:Lrrn4 APN 2 132870061 missense possibly damaging 0.79
R0270:Lrrn4 UTSW 2 132870719 missense probably benign 0.01
R0348:Lrrn4 UTSW 2 132870443 missense probably benign 0.02
R0400:Lrrn4 UTSW 2 132878020 missense probably benign 0.12
R0701:Lrrn4 UTSW 2 132870160 missense probably benign 0.02
R1465:Lrrn4 UTSW 2 132872075 missense probably damaging 1.00
R1465:Lrrn4 UTSW 2 132872075 missense probably damaging 1.00
R1664:Lrrn4 UTSW 2 132869966 missense probably damaging 1.00
R1987:Lrrn4 UTSW 2 132870443 missense probably benign 0.02
R3409:Lrrn4 UTSW 2 132879861 missense unknown
R3743:Lrrn4 UTSW 2 132869866 splice site probably null
R4678:Lrrn4 UTSW 2 132879568 missense probably benign 0.16
R5770:Lrrn4 UTSW 2 132872156 missense probably damaging 1.00
R6438:Lrrn4 UTSW 2 132870142 missense probably damaging 1.00
R6511:Lrrn4 UTSW 2 132870326 missense probably benign 0.06
R6880:Lrrn4 UTSW 2 132872112 missense probably damaging 0.96
R7132:Lrrn4 UTSW 2 132879693 nonsense probably null
R7273:Lrrn4 UTSW 2 132879829 missense unknown
R7424:Lrrn4 UTSW 2 132869743 missense possibly damaging 0.90
R7980:Lrrn4 UTSW 2 132878176 missense probably damaging 1.00
R8133:Lrrn4 UTSW 2 132878014 missense probably damaging 1.00
R8229:Lrrn4 UTSW 2 132869887 missense probably damaging 1.00
R8489:Lrrn4 UTSW 2 132879444 missense probably benign 0.20
R8956:Lrrn4 UTSW 2 132872091 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TCAGATCCAAGAGCTCCAGTG -3'
(R):5'- CCACTCTGAACTTGGCGAAC -3'

Sequencing Primer
(F):5'- CTCGCTGCAGGAGAGGTTGAG -3'
(R):5'- AACCGCAGCCTGGAGAG -3'
Posted On2019-11-12