Incidental Mutation 'R0653:Dagla'
ID62414
Institutional Source Beutler Lab
Gene Symbol Dagla
Ensembl Gene ENSMUSG00000035735
Gene Namediacylglycerol lipase, alpha
SynonymsNsddr
MMRRC Submission 038838-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R0653 (G1)
Quality Score96
Status Not validated
Chromosome19
Chromosomal Location10245265-10304877 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 10248425 bp
ZygosityHeterozygous
Amino Acid Change Tyrosine to Histidine at position 792 (Y792H)
Ref Sequence ENSEMBL: ENSMUSP00000046358 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000039327] [ENSMUST00000125567]
Predicted Effect probably damaging
Transcript: ENSMUST00000039327
AA Change: Y792H

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000046358
Gene: ENSMUSG00000035735
AA Change: Y792H

DomainStartEndE-ValueType
transmembrane domain 21 43 N/A INTRINSIC
transmembrane domain 63 85 N/A INTRINSIC
transmembrane domain 97 119 N/A INTRINSIC
transmembrane domain 134 156 N/A INTRINSIC
Pfam:Lipase_3 394 533 1.3e-12 PFAM
low complexity region 616 625 N/A INTRINSIC
low complexity region 699 717 N/A INTRINSIC
low complexity region 793 810 N/A INTRINSIC
low complexity region 878 896 N/A INTRINSIC
low complexity region 980 1002 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000125567
SMART Domains Protein: ENSMUSP00000138702
Gene: ENSMUSG00000035735

DomainStartEndE-ValueType
transmembrane domain 20 42 N/A INTRINSIC
transmembrane domain 63 85 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000156361
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.9%
  • 10x: 97.6%
  • 20x: 95.4%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a diacylglycerol lipase. The encoded enzyme is involved in the biosynthesis of the endocannabinoid 2-arachidonoyl-glycerol.[provided by RefSeq, Nov 2010]
PHENOTYPE: Mice homozygous for null mutations have decreased body weight, adult neuronal proliferation, and nervous system endocannaboid levels and abnormal inhibitory postsynaptic currents. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 55 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4933427D14Rik T A 11: 72,175,545 R612* probably null Het
Adgra1 A G 7: 139,876,147 T564A probably damaging Het
Akr1c18 A T 13: 4,145,308 D50E probably damaging Het
Atg9a C A 1: 75,190,328 L26F probably damaging Het
Atp11b A G 3: 35,839,194 T899A probably damaging Het
Atxn2 A G 5: 121,772,778 E358G probably damaging Het
Bmp3 A G 5: 98,872,111 Y131C probably damaging Het
Brip1 T C 11: 86,152,658 E360G possibly damaging Het
Casd1 A T 6: 4,608,075 I76L probably benign Het
Casq2 G A 3: 102,113,166 probably null Het
Ccdc185 T A 1: 182,747,564 Q520L possibly damaging Het
Cenpf T C 1: 189,659,986 K550E probably damaging Het
Ciz1 T C 2: 32,372,406 S521P probably damaging Het
Clstn2 A T 9: 97,458,204 V705E probably damaging Het
Dgke A T 11: 89,060,169 C73S probably benign Het
Egflam A G 15: 7,250,028 probably null Het
Farp1 T C 14: 121,233,846 probably null Het
Fos A G 12: 85,476,016 E234G probably benign Het
Gtf3c5 A T 2: 28,577,996 M151K probably benign Het
Hgs G A 11: 120,469,078 R36H probably damaging Het
Lats2 G A 14: 57,700,196 Q279* probably null Het
Lysmd4 T A 7: 67,226,040 D150E probably benign Het
Mex3b A G 7: 82,869,034 K186E probably damaging Het
Myo9a A C 9: 59,924,991 Q2601P probably damaging Het
Nckap5l C A 15: 99,423,246 V1218F probably damaging Het
Nr5a2 A G 1: 136,948,805 V40A probably benign Het
Obscn C A 11: 59,007,708 probably benign Het
Olfr1219 A G 2: 89,074,464 I209T possibly damaging Het
Olfr1389 C A 11: 49,431,251 Y258* probably null Het
Olfr967 A G 9: 39,750,638 N84S probably benign Het
Pclo T A 5: 14,682,255 probably benign Het
Reln T C 5: 21,913,230 I2939V probably benign Het
Rtn4 A T 11: 29,707,256 K470I probably damaging Het
Sbno1 A G 5: 124,386,892 I1050T possibly damaging Het
Scaf11 G T 15: 96,418,641 S17* probably null Het
Scn9a A T 2: 66,533,377 N841K probably damaging Het
Slc35e3 C A 10: 117,740,806 E207* probably null Het
Slc6a20b A G 9: 123,597,312 F503L probably damaging Het
Spag16 G T 1: 69,870,345 K200N probably damaging Het
Supt6 G T 11: 78,226,015 Q604K probably benign Het
Taok1 A G 11: 77,578,724 probably null Het
Tjp1 A T 7: 65,314,755 H889Q probably damaging Het
Tmed6 A T 8: 107,065,651 probably null Het
Tsen54 A T 11: 115,815,061 E68V probably damaging Het
Ttn A G 2: 76,729,534 S21181P probably damaging Het
Ube2cbp A T 9: 86,451,990 M33K possibly damaging Het
Umodl1 C A 17: 30,984,028 Q452K probably benign Het
Wif1 G T 10: 121,099,799 A354S probably benign Het
Wnk2 A G 13: 49,057,016 F1788L possibly damaging Het
Zfhx3 A T 8: 108,946,808 I1497F possibly damaging Het
Zfp217 C T 2: 170,115,462 A539T probably benign Het
Zfp30 G A 7: 29,792,753 R225Q probably damaging Het
Zfp72 T C 13: 74,372,071 E296G probably damaging Het
Zfp874b A G 13: 67,474,933 F86S possibly damaging Het
Zfyve19 A G 2: 119,211,215 S88G probably benign Het
Other mutations in Dagla
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01484:Dagla APN 19 10248520 missense possibly damaging 0.51
IGL01625:Dagla APN 19 10251202 splice site probably benign
IGL01697:Dagla APN 19 10271198 missense probably benign 0.01
IGL01940:Dagla APN 19 10252171 missense probably benign
IGL02330:Dagla APN 19 10248022 missense probably damaging 1.00
PIT4480001:Dagla UTSW 19 10260658 missense probably benign 0.02
R0541:Dagla UTSW 19 10254806 critical splice donor site probably null
R0610:Dagla UTSW 19 10271558 missense probably damaging 1.00
R0638:Dagla UTSW 19 10254883 missense probably damaging 0.97
R1675:Dagla UTSW 19 10269323 missense probably benign 0.00
R1822:Dagla UTSW 19 10263186 missense possibly damaging 0.94
R1830:Dagla UTSW 19 10271014 missense probably benign 0.44
R2303:Dagla UTSW 19 10252103 missense probably damaging 1.00
R2568:Dagla UTSW 19 10248152 missense probably benign
R2879:Dagla UTSW 19 10271084 missense possibly damaging 0.93
R2902:Dagla UTSW 19 10248103 missense probably damaging 0.99
R2939:Dagla UTSW 19 10256364 missense probably damaging 1.00
R3771:Dagla UTSW 19 10248467 missense possibly damaging 0.89
R4176:Dagla UTSW 19 10263097 missense probably damaging 1.00
R4255:Dagla UTSW 19 10256952 nonsense probably null
R4519:Dagla UTSW 19 10269732 missense probably damaging 1.00
R4584:Dagla UTSW 19 10271009 missense probably damaging 1.00
R4586:Dagla UTSW 19 10271009 missense probably damaging 1.00
R4614:Dagla UTSW 19 10248277 missense probably damaging 1.00
R4751:Dagla UTSW 19 10250394 missense probably benign 0.00
R4933:Dagla UTSW 19 10269715 critical splice donor site probably null
R5844:Dagla UTSW 19 10271125 missense probably damaging 1.00
R5858:Dagla UTSW 19 10254968 intron probably benign
R5958:Dagla UTSW 19 10248424 missense probably damaging 1.00
R6628:Dagla UTSW 19 10263227 missense probably damaging 1.00
R6799:Dagla UTSW 19 10256850 missense probably damaging 1.00
R7072:Dagla UTSW 19 10256295 critical splice donor site probably null
R7253:Dagla UTSW 19 10262581 splice site probably null
R7451:Dagla UTSW 19 10253355 missense probably damaging 1.00
R7654:Dagla UTSW 19 10248206 missense probably benign 0.01
X0021:Dagla UTSW 19 10271164 missense probably benign
Predicted Primers PCR Primer
(F):5'- TCAATGAACTCGGCGAATTCTAGCAC -3'
(R):5'- TTCCTTCTAGCCAGGGAAGTAGGGAC -3'

Sequencing Primer
(F):5'- GGCGAATTCTAGCACCTGAG -3'
(R):5'- CTGTAGGAGCAAGTCTCAGTC -3'
Posted On2013-07-30