Mutagenetix > Incidental Mutation

Incidental Mutation 'R8120:Zfp267'

631438

Institutional Source

Beutler Lab

Gene Symbol

Zfp267

Ensembl Gene

ENSMUSG00000033883

Gene Name

zinc finger protein 267

Synonyms

D3Ertd254e

MMRRC Submission

067549-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.128) question?

Stock #

R8120 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

36205233-36224491 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 36218640 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Isoleucine at position 221 (N221I)

Ref Sequence

ENSEMBL: ENSMUSP00000142829 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165956] [ENSMUST00000197653] [ENSMUST00000205077]

AlphaFold

A0A0G2JEM5

Predicted Effect

possibly damaging
Transcript: ENSMUST00000165956
AA Change: N220I

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000131779
Gene: ENSMUSG00000033883
AA Change: N220I

Domain	Start	End	E-Value	Type
KRAB	3	63	2.91e-34	SMART
ZnF_C2H2	342	364	1.08e-1	SMART
ZnF_C2H2	395	417	1.56e-2	SMART
ZnF_C2H2	423	445	3.11e-2	SMART
ZnF_C2H2	451	473	5.9e-3	SMART
ZnF_C2H2	479	501	1.82e-3	SMART
ZnF_C2H2	507	529	5.21e-4	SMART
ZnF_C2H2	535	557	1.84e-4	SMART
ZnF_C2H2	563	585	1.95e-3	SMART
ZnF_C2H2	591	613	2.05e-2	SMART
ZnF_C2H2	619	641	1.6e-4	SMART
ZnF_C2H2	647	669	5.21e-4	SMART
ZnF_C2H2	675	697	1.69e-3	SMART
ZnF_C2H2	703	725	2.61e-4	SMART
ZnF_C2H2	731	753	1.12e-3	SMART
ZnF_C2H2	759	779	3.85e1	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000197653
AA Change: N221I

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000142829
Gene: ENSMUSG00000033883
AA Change: N221I

Domain	Start	End	E-Value	Type
KRAB	4	64	1.2e-36	SMART
ZnF_C2H2	343	365	4.4e-4	SMART
ZnF_C2H2	396	418	6.7e-5	SMART
ZnF_C2H2	424	446	1.3e-4	SMART
ZnF_C2H2	452	474	2.5e-5	SMART
ZnF_C2H2	480	502	7.9e-6	SMART
ZnF_C2H2	508	530	2.2e-6	SMART
ZnF_C2H2	536	558	7.7e-7	SMART
ZnF_C2H2	564	586	8e-6	SMART
ZnF_C2H2	592	614	8.9e-5	SMART
ZnF_C2H2	620	642	6.6e-7	SMART
ZnF_C2H2	648	670	2.2e-6	SMART
ZnF_C2H2	676	698	7.1e-6	SMART
ZnF_C2H2	704	726	1.1e-6	SMART
ZnF_C2H2	732	754	4.8e-6	SMART
ZnF_C2H2	760	780	1.6e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000205077

Meta Mutation Damage Score

0.1712

Coding Region Coverage

1x: 99.8%
3x: 99.6%
10x: 98.6%
20x: 95.1%

Validation Efficiency

100% (57/57)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl4	A	T	4: 144,349,460 (GRCm39)	Q239L	probably benign	Het
Abca12	T	C	1: 71,298,540 (GRCm39)	I2361V	possibly damaging	Het
Abcc8	T	C	7: 45,786,108 (GRCm39)	E655G	probably benign	Het
Ablim1	C	T	19: 57,035,360 (GRCm39)	V662I	probably benign	Het
Acte1	A	G	7: 143,425,524 (GRCm39)	T58A	probably benign	Het
Als2cl	A	C	9: 110,714,460 (GRCm39)	I103L	possibly damaging	Het
Arhgef26	A	T	3: 62,248,796 (GRCm39)	D370V	probably damaging	Het
Brwd1	A	C	16: 95,820,649 (GRCm39)	D1292E	probably benign	Het
Cdc5l	T	C	17: 45,718,796 (GRCm39)	T607A	probably benign	Het
Cfhr1	T	C	1: 139,475,583 (GRCm39)	Y296C	unknown	Het
Cubn	A	T	2: 13,336,471 (GRCm39)	C2452S	probably damaging	Het
Dgcr2	A	T	16: 17,675,183 (GRCm39)	Y187*	probably null	Het
Dhodh	G	A	8: 110,328,057 (GRCm39)	T172I	probably benign	Het
Dnah6	G	A	6: 73,002,769 (GRCm39)	R3876C	probably damaging	Het
Dnai4	G	A	4: 102,923,531 (GRCm39)	R433W	probably damaging	Het
Farsb	A	T	1: 78,439,475 (GRCm39)	N389K	probably benign	Het
Fryl	T	C	5: 73,228,527 (GRCm39)	T1735A	probably benign	Het
Garin4	T	G	1: 190,895,022 (GRCm39)	Q540H	probably damaging	Het
Gpcpd1	C	T	2: 132,395,943 (GRCm39)	R136H	probably damaging	Het
Gpr171	T	A	3: 59,005,406 (GRCm39)	Y123F	probably damaging	Het
Hcar1	C	A	5: 124,017,068 (GRCm39)	V208F	probably damaging	Het
Hgf	T	A	5: 16,818,779 (GRCm39)	L524M	probably damaging	Het
Itga2b	A	T	11: 102,360,368 (GRCm39)	H57Q	probably damaging	Het
Itpripl2	G	T	7: 118,089,508 (GRCm39)	N350K	probably damaging	Het
Limk2	C	T	11: 3,298,589 (GRCm39)		probably null	Het
Nae1	A	T	8: 105,246,267 (GRCm39)	V315E	probably damaging	Het
Nars1	T	C	18: 64,637,422 (GRCm39)	Y386C	probably benign	Het
Odr4	T	C	1: 150,260,177 (GRCm39)		probably null	Het
Or10j3	C	T	1: 173,031,502 (GRCm39)	T193I	probably benign	Het
Or6z5	A	T	7: 6,477,119 (GRCm39)	R3S	probably benign	Het
Pcnx3	C	T	19: 5,717,574 (GRCm39)	A1512T	probably benign	Het
Pde1b	A	G	15: 103,430,524 (GRCm39)	D176G	possibly damaging	Het
Pde4dip	T	A	3: 97,614,254 (GRCm39)	T1855S	probably null	Het
Prr12	A	G	7: 44,684,166 (GRCm39)	Y1625H	probably damaging	Het
Psmc2	T	C	5: 22,005,566 (GRCm39)	Y216H	probably damaging	Het
Ptchd3	A	T	11: 121,733,034 (GRCm39)	E641D	probably benign	Het
Rtf1	A	G	2: 119,531,602 (GRCm39)	T110A	probably damaging	Het
Rusc1	C	A	3: 88,996,513 (GRCm39)	W690L	probably damaging	Het
Sardh	A	G	2: 27,108,863 (GRCm39)	V624A	possibly damaging	Het
Slc17a9	G	A	2: 180,374,308 (GRCm39)	G125S	probably benign	Het
Smpdl3a	A	T	10: 57,683,547 (GRCm39)	N219I	probably damaging	Het
Spo11	G	T	2: 172,827,251 (GRCm39)	D155Y	probably damaging	Het
Stk38l	A	G	6: 146,660,099 (GRCm39)	T44A	probably benign	Het
Tceanc2	A	T	4: 107,034,829 (GRCm39)	I11N	probably benign	Het
Tead2	A	T	7: 44,865,752 (GRCm39)		probably benign	Het
Tpk1	A	G	6: 43,445,930 (GRCm39)		probably null	Het
Ttn	T	C	2: 76,593,835 (GRCm39)	N20602D	probably damaging	Het
Tub	T	A	7: 108,624,803 (GRCm39)		probably null	Het
Uba2	A	T	7: 33,867,812 (GRCm39)	S42T	probably benign	Het
Vangl1	T	A	3: 102,070,758 (GRCm39)	R393*	probably null	Het
Washc3	G	A	10: 88,037,159 (GRCm39)		probably null	Het
Wipf1	G	A	2: 73,267,879 (GRCm39)	P173L	possibly damaging	Het
Zfp217	G	A	2: 169,961,571 (GRCm39)	S252F	possibly damaging	Het
Zfp386	C	T	12: 116,018,573 (GRCm39)	P81S	unknown	Het
Zw10	A	T	9: 48,985,413 (GRCm39)	E616D	probably benign	Het

Other mutations in Zfp267

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01524:Zfp267	APN	3	36,218,729 (GRCm39)	missense	possibly damaging	0.86
IGL02089:Zfp267	APN	3	36,218,877 (GRCm39)	missense	possibly damaging	0.53
IGL02162:Zfp267	APN	3	36,218,210 (GRCm39)	missense	probably benign	0.18
R0243:Zfp267	UTSW	3	36,219,303 (GRCm39)	missense	possibly damaging	0.47
R0512:Zfp267	UTSW	3	36,220,262 (GRCm39)	missense	probably damaging	0.96
R0722:Zfp267	UTSW	3	36,219,218 (GRCm39)	missense	probably benign	0.35
R0762:Zfp267	UTSW	3	36,220,016 (GRCm39)	missense	possibly damaging	0.92
R0792:Zfp267	UTSW	3	36,218,711 (GRCm39)	missense	probably benign	0.01
R0894:Zfp267	UTSW	3	36,218,935 (GRCm39)	nonsense	probably null
R1731:Zfp267	UTSW	3	36,218,620 (GRCm39)	missense	probably benign	0.18
R2098:Zfp267	UTSW	3	36,220,289 (GRCm39)	missense	probably benign
R2099:Zfp267	UTSW	3	36,218,361 (GRCm39)	missense	possibly damaging	0.86
R3709:Zfp267	UTSW	3	36,213,725 (GRCm39)	missense	possibly damaging	0.71
R3808:Zfp267	UTSW	3	36,219,792 (GRCm39)	splice site	probably null
R4035:Zfp267	UTSW	3	36,218,989 (GRCm39)	missense	possibly damaging	0.53
R4288:Zfp267	UTSW	3	36,213,747 (GRCm39)	missense	possibly damaging	0.71
R4289:Zfp267	UTSW	3	36,213,747 (GRCm39)	missense	possibly damaging	0.71
R4959:Zfp267	UTSW	3	36,218,285 (GRCm39)	missense	possibly damaging	0.91
R4973:Zfp267	UTSW	3	36,218,285 (GRCm39)	missense	possibly damaging	0.91
R5102:Zfp267	UTSW	3	36,216,814 (GRCm39)	missense	possibly damaging	0.73
R5462:Zfp267	UTSW	3	36,219,969 (GRCm39)	missense	possibly damaging	0.95
R5548:Zfp267	UTSW	3	36,219,640 (GRCm39)	missense	possibly damaging	0.90
R5782:Zfp267	UTSW	3	36,219,128 (GRCm39)	missense	possibly damaging	0.73
R6153:Zfp267	UTSW	3	36,219,303 (GRCm39)	missense	possibly damaging	0.47
R6225:Zfp267	UTSW	3	36,220,352 (GRCm39)	missense	probably benign	0.18
R6602:Zfp267	UTSW	3	36,219,004 (GRCm39)	missense	possibly damaging	0.86
R6785:Zfp267	UTSW	3	36,219,601 (GRCm39)	nonsense	probably null
R7513:Zfp267	UTSW	3	36,218,792 (GRCm39)	missense	possibly damaging	0.53
R7846:Zfp267	UTSW	3	36,219,738 (GRCm39)	missense	probably benign	0.43
R8265:Zfp267	UTSW	3	36,213,677 (GRCm39)	start gained	probably benign
R8415:Zfp267	UTSW	3	36,219,182 (GRCm39)	missense	probably damaging	0.98
R8826:Zfp267	UTSW	3	36,218,255 (GRCm39)	missense	possibly damaging	0.86
R9026:Zfp267	UTSW	3	36,219,066 (GRCm39)	missense	possibly damaging	0.96
R9159:Zfp267	UTSW	3	36,219,902 (GRCm39)	missense	possibly damaging	0.47
R9786:Zfp267	UTSW	3	36,219,853 (GRCm39)	nonsense	probably null
X0021:Zfp267	UTSW	3	36,218,340 (GRCm39)	missense	possibly damaging	0.72

Predicted Primers

PCR Primer
(F):5'- TTTACTCCCAGGAGAGATCGG -3'
(R):5'- TGGTTCAGTGATACCGGATG -3'

Sequencing Primer
(F):5'- TCAGGTGACACCAGTTACTTG -3'
(R):5'- TTTGGCACATGGAAGACC -3'

Posted On

2020-06-30