Mutagenetix > Incidental Mutation

Incidental Mutation 'R1731:Zfp267'

199290

Institutional Source

Beutler Lab

Gene Symbol

Zfp267

Ensembl Gene

ENSMUSG00000033883

Gene Name

zinc finger protein 267

Synonyms

D3Ertd254e

MMRRC Submission

039763-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.128) question?

Stock #

R1731 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

36205233-36224491 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 36218620 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 214 (F214L)

Ref Sequence

ENSEMBL: ENSMUSP00000142829 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165956] [ENSMUST00000197653] [ENSMUST00000205077]

AlphaFold

A0A0G2JEM5

Predicted Effect

probably benign
Transcript: ENSMUST00000165956
AA Change: F213L

PolyPhen 2 Score 0.177 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000131779
Gene: ENSMUSG00000033883
AA Change: F213L

Domain	Start	End	E-Value	Type
KRAB	3	63	2.91e-34	SMART
ZnF_C2H2	342	364	1.08e-1	SMART
ZnF_C2H2	395	417	1.56e-2	SMART
ZnF_C2H2	423	445	3.11e-2	SMART
ZnF_C2H2	451	473	5.9e-3	SMART
ZnF_C2H2	479	501	1.82e-3	SMART
ZnF_C2H2	507	529	5.21e-4	SMART
ZnF_C2H2	535	557	1.84e-4	SMART
ZnF_C2H2	563	585	1.95e-3	SMART
ZnF_C2H2	591	613	2.05e-2	SMART
ZnF_C2H2	619	641	1.6e-4	SMART
ZnF_C2H2	647	669	5.21e-4	SMART
ZnF_C2H2	675	697	1.69e-3	SMART
ZnF_C2H2	703	725	2.61e-4	SMART
ZnF_C2H2	731	753	1.12e-3	SMART
ZnF_C2H2	759	779	3.85e1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000197653
AA Change: F214L

PolyPhen 2 Score 0.177 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000142829
Gene: ENSMUSG00000033883
AA Change: F214L

Domain	Start	End	E-Value	Type
KRAB	4	64	1.2e-36	SMART
ZnF_C2H2	343	365	4.4e-4	SMART
ZnF_C2H2	396	418	6.7e-5	SMART
ZnF_C2H2	424	446	1.3e-4	SMART
ZnF_C2H2	452	474	2.5e-5	SMART
ZnF_C2H2	480	502	7.9e-6	SMART
ZnF_C2H2	508	530	2.2e-6	SMART
ZnF_C2H2	536	558	7.7e-7	SMART
ZnF_C2H2	564	586	8e-6	SMART
ZnF_C2H2	592	614	8.9e-5	SMART
ZnF_C2H2	620	642	6.6e-7	SMART
ZnF_C2H2	648	670	2.2e-6	SMART
ZnF_C2H2	676	698	7.1e-6	SMART
ZnF_C2H2	704	726	1.1e-6	SMART
ZnF_C2H2	732	754	4.8e-6	SMART
ZnF_C2H2	760	780	1.6e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000205077

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.3%
20x: 92.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam7	T	A	14: 68,762,805 (GRCm39)	Y140F	probably damaging	Het
Adgrl4	T	G	3: 151,246,623 (GRCm39)	I641S	possibly damaging	Het
Aqp9	A	G	9: 71,030,250 (GRCm39)	I205T	possibly damaging	Het
Arap3	C	T	18: 38,122,965 (GRCm39)	V512I	probably benign	Het
Atf2	C	T	2: 73,675,853 (GRCm39)	G123E	probably damaging	Het
Baz1a	T	C	12: 54,965,330 (GRCm39)	D708G	possibly damaging	Het
Calcrl	A	G	2: 84,175,512 (GRCm39)		probably null	Het
Capzb	T	A	4: 139,007,341 (GRCm39)	W110R	probably damaging	Het
Casp8ap2	A	T	4: 32,641,442 (GRCm39)	N832I	possibly damaging	Het
Cecr2	T	A	6: 120,735,141 (GRCm39)	H764Q	possibly damaging	Het
Cep131	T	C	11: 119,967,742 (GRCm39)		probably null	Het
Ces2e	T	C	8: 105,656,208 (GRCm39)	V173A	probably damaging	Het
Clstn3	T	C	6: 124,408,591 (GRCm39)	D944G	probably benign	Het
Cyb5rl	C	A	4: 106,938,110 (GRCm39)	A189E	probably damaging	Het
Cyp2c40	A	G	19: 39,801,133 (GRCm39)	S41P	probably damaging	Het
Dscam	A	G	16: 96,621,076 (GRCm39)	L544P	probably damaging	Het
Epha2	A	G	4: 141,049,063 (GRCm39)	K640E	possibly damaging	Het
Erap1	T	A	13: 74,814,241 (GRCm39)	C8*	probably null	Het
Fat3	A	G	9: 15,907,233 (GRCm39)	V2923A	probably benign	Het
Fat4	A	T	3: 38,945,459 (GRCm39)	I1451F	probably damaging	Het
Fcsk	A	T	8: 111,621,455 (GRCm39)	I163N	probably damaging	Het
Fzd6	G	A	15: 38,894,722 (GRCm39)	G296D	probably damaging	Het
Gm3486	T	A	14: 41,206,492 (GRCm39)	M194L	probably benign	Het
Gm5592	C	T	7: 40,937,837 (GRCm39)	A373V	probably damaging	Het
Hectd3	T	A	4: 116,853,652 (GRCm39)		probably null	Het
Hira	T	A	16: 18,751,764 (GRCm39)	V521E	probably benign	Het
Hsd17b6	A	G	10: 127,830,348 (GRCm39)	L141S	possibly damaging	Het
Idua	A	G	5: 108,829,538 (GRCm39)	D467G	probably benign	Het
Ikzf4	G	A	10: 128,470,401 (GRCm39)	P373L	probably benign	Het
Kcng1	T	C	2: 168,110,609 (GRCm39)	E185G	probably benign	Het
Krt84	G	A	15: 101,434,398 (GRCm39)	S523F	possibly damaging	Het
Lpcat4	T	C	2: 112,074,188 (GRCm39)	L250P	probably damaging	Het
Lrrc49	A	G	9: 60,528,914 (GRCm39)	Y281H	probably damaging	Het
Mta2	C	A	19: 8,925,088 (GRCm39)		probably null	Het
Myo15b	T	C	11: 115,782,386 (GRCm39)	I372T	possibly damaging	Het
Myocd	T	A	11: 65,091,714 (GRCm39)	N76I	probably benign	Het
Nav2	T	A	7: 49,197,922 (GRCm39)	Y1123N	probably damaging	Het
Otogl	A	T	10: 107,652,972 (GRCm39)	C1127S	probably damaging	Het
Pcdhb8	A	G	18: 37,488,891 (GRCm39)	K190E	probably damaging	Het
Pcnx1	A	G	12: 82,037,478 (GRCm39)	H1918R	probably damaging	Het
Pde2a	T	A	7: 101,150,867 (GRCm39)	Y272N	probably damaging	Het
Phldb3	T	C	7: 24,318,660 (GRCm39)	V313A	probably benign	Het
Plch2	C	T	4: 155,091,451 (GRCm39)	V116I	possibly damaging	Het
Plod2	G	A	9: 92,466,657 (GRCm39)		probably null	Het
Ppfibp2	T	C	7: 107,339,796 (GRCm39)	Y730H	probably damaging	Het
Ptprh	T	C	7: 4,604,912 (GRCm39)	E44G	probably benign	Het
Rab11fip1	T	C	8: 27,642,438 (GRCm39)	E787G	probably damaging	Het
Rabep2	T	A	7: 126,043,444 (GRCm39)	L448Q	probably damaging	Het
Rbfox3	A	G	11: 118,387,762 (GRCm39)		probably null	Het
Rgma	T	C	7: 73,059,160 (GRCm39)	V88A	probably damaging	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Rnf125	A	G	18: 21,110,873 (GRCm39)	T44A	probably benign	Het
Rusc2	G	T	4: 43,426,046 (GRCm39)	A1384S	probably benign	Het
Selp	T	A	1: 163,969,009 (GRCm39)	C536*	probably null	Het
Serpinb3c	G	A	1: 107,199,504 (GRCm39)	T339I	probably damaging	Het
Slc4a5	C	T	6: 83,273,617 (GRCm39)	R986C	probably damaging	Het
Slc8b1	T	C	5: 120,659,180 (GRCm39)	I208T	probably benign	Het
Sp3	G	A	2: 72,776,999 (GRCm39)	H533Y	probably damaging	Het
Speer1e	T	A	5: 11,233,080 (GRCm39)	N14K	probably damaging	Het
Tiam2	A	G	17: 3,568,698 (GRCm39)	R1615G	probably damaging	Het
Tie1	T	C	4: 118,333,460 (GRCm39)	E802G	probably damaging	Het
Tinagl1	A	G	4: 130,061,842 (GRCm39)	V164A	probably benign	Het
Vmn1r68	A	G	7: 10,261,802 (GRCm39)	Y99H	probably damaging	Het
Vmn1r87	T	A	7: 12,865,703 (GRCm39)	T195S	possibly damaging	Het
Vmn2r56	T	C	7: 12,466,972 (GRCm39)	T21A	probably benign	Het
Zfp108	C	A	7: 23,957,964 (GRCm39)	H34Q	possibly damaging	Het
Zfp456	C	T	13: 67,514,674 (GRCm39)	S344N	probably benign	Het
Zscan22	T	C	7: 12,640,907 (GRCm39)	C384R	probably damaging	Het

Other mutations in Zfp267

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01524:Zfp267	APN	3	36,218,729 (GRCm39)	missense	possibly damaging	0.86
IGL02089:Zfp267	APN	3	36,218,877 (GRCm39)	missense	possibly damaging	0.53
IGL02162:Zfp267	APN	3	36,218,210 (GRCm39)	missense	probably benign	0.18
R0243:Zfp267	UTSW	3	36,219,303 (GRCm39)	missense	possibly damaging	0.47
R0512:Zfp267	UTSW	3	36,220,262 (GRCm39)	missense	probably damaging	0.96
R0722:Zfp267	UTSW	3	36,219,218 (GRCm39)	missense	probably benign	0.35
R0762:Zfp267	UTSW	3	36,220,016 (GRCm39)	missense	possibly damaging	0.92
R0792:Zfp267	UTSW	3	36,218,711 (GRCm39)	missense	probably benign	0.01
R0894:Zfp267	UTSW	3	36,218,935 (GRCm39)	nonsense	probably null
R2098:Zfp267	UTSW	3	36,220,289 (GRCm39)	missense	probably benign
R2099:Zfp267	UTSW	3	36,218,361 (GRCm39)	missense	possibly damaging	0.86
R3709:Zfp267	UTSW	3	36,213,725 (GRCm39)	missense	possibly damaging	0.71
R3808:Zfp267	UTSW	3	36,219,792 (GRCm39)	splice site	probably null
R4035:Zfp267	UTSW	3	36,218,989 (GRCm39)	missense	possibly damaging	0.53
R4288:Zfp267	UTSW	3	36,213,747 (GRCm39)	missense	possibly damaging	0.71
R4289:Zfp267	UTSW	3	36,213,747 (GRCm39)	missense	possibly damaging	0.71
R4959:Zfp267	UTSW	3	36,218,285 (GRCm39)	missense	possibly damaging	0.91
R4973:Zfp267	UTSW	3	36,218,285 (GRCm39)	missense	possibly damaging	0.91
R5102:Zfp267	UTSW	3	36,216,814 (GRCm39)	missense	possibly damaging	0.73
R5462:Zfp267	UTSW	3	36,219,969 (GRCm39)	missense	possibly damaging	0.95
R5548:Zfp267	UTSW	3	36,219,640 (GRCm39)	missense	possibly damaging	0.90
R5782:Zfp267	UTSW	3	36,219,128 (GRCm39)	missense	possibly damaging	0.73
R6153:Zfp267	UTSW	3	36,219,303 (GRCm39)	missense	possibly damaging	0.47
R6225:Zfp267	UTSW	3	36,220,352 (GRCm39)	missense	probably benign	0.18
R6602:Zfp267	UTSW	3	36,219,004 (GRCm39)	missense	possibly damaging	0.86
R6785:Zfp267	UTSW	3	36,219,601 (GRCm39)	nonsense	probably null
R7513:Zfp267	UTSW	3	36,218,792 (GRCm39)	missense	possibly damaging	0.53
R7846:Zfp267	UTSW	3	36,219,738 (GRCm39)	missense	probably benign	0.43
R8120:Zfp267	UTSW	3	36,218,640 (GRCm39)	missense	possibly damaging	0.96
R8265:Zfp267	UTSW	3	36,213,677 (GRCm39)	start gained	probably benign
R8415:Zfp267	UTSW	3	36,219,182 (GRCm39)	missense	probably damaging	0.98
R8826:Zfp267	UTSW	3	36,218,255 (GRCm39)	missense	possibly damaging	0.86
R9026:Zfp267	UTSW	3	36,219,066 (GRCm39)	missense	possibly damaging	0.96
R9159:Zfp267	UTSW	3	36,219,902 (GRCm39)	missense	possibly damaging	0.47
R9786:Zfp267	UTSW	3	36,219,853 (GRCm39)	nonsense	probably null
X0021:Zfp267	UTSW	3	36,218,340 (GRCm39)	missense	possibly damaging	0.72

Predicted Primers

PCR Primer
(F):5'- ACGGGCACAACCCATTTCTGAC -3'
(R):5'- TAAGCTGGACTCCTCAGTGAAGGC -3'

Sequencing Primer
(F):5'- CTTTACTCCCAGGAGAGATCG -3'
(R):5'- TACCGGATGTGAAAAAAATCTGCC -3'

Posted On

2014-05-23