Mutagenetix > Incidental Mutation

Incidental Mutation 'R8758:Vmn2r57'

671739

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r57

Ensembl Gene

ENSMUSG00000066537

Gene Name

vomeronasal 2, receptor 57

Synonyms

EG269902

MMRRC Submission

068598-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.106) question?

Stock #

R8758 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

41049156-41098065 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 41078163 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 98 (N98K)

Ref Sequence

ENSEMBL: ENSMUSP00000125817 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094532] [ENSMUST00000165029]

AlphaFold

L7N269

Predicted Effect

probably benign
Transcript: ENSMUST00000094532

Predicted Effect

probably damaging
Transcript: ENSMUST00000165029
AA Change: N98K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000125817
Gene: ENSMUSG00000066537
AA Change: N98K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	1.4e-44	PFAM
Pfam:NCD3G	514	567	2.7e-23	PFAM
Pfam:7tm_3	600	835	1.8e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700009N14Rik	G	A	4: 39,450,811 (GRCm39)	D6N	probably benign	Het
AC157566.4	T	C	15: 76,418,382 (GRCm39)	I42M	probably benign	Het
Amer2	C	T	14: 60,616,326 (GRCm39)	R174C	probably damaging	Het
Arl8a	A	T	1: 135,082,189 (GRCm39)	D139V	possibly damaging	Het
Csmd3	A	G	15: 47,469,593 (GRCm39)	Y2525H		Het
Cwc22	T	C	2: 77,747,441 (GRCm39)	E424G	possibly damaging	Het
D930048N14Rik	A	G	11: 51,544,568 (GRCm39)	T80A	unknown	Het
Ddx25	T	A	9: 35,453,300 (GRCm39)	E483D	probably benign	Het
Dock4	A	T	12: 40,838,231 (GRCm39)	E1057D	probably benign	Het
Dock7	C	T	4: 98,949,555 (GRCm39)	R429Q		Het
Dzip3	T	C	16: 48,798,300 (GRCm39)	Y135C	probably damaging	Het
Evi5l	G	T	8: 4,255,860 (GRCm39)	A618S	probably benign	Het
Fibp	A	G	19: 5,514,970 (GRCm39)	D348G	possibly damaging	Het
Gimd1	T	A	3: 132,340,799 (GRCm39)	L105H	probably damaging	Het
Glra1	T	A	11: 55,418,191 (GRCm39)	I268F	possibly damaging	Het
Gm30191	A	G	4: 133,977,099 (GRCm39)	T104A	probably benign	Het
Gm49359	A	T	13: 62,602,150 (GRCm39)	I350K	possibly damaging	Het
Gpm6a	T	C	8: 55,511,833 (GRCm39)	S236P	probably damaging	Het
Igkv4-74	AG	A	6: 69,162,311 (GRCm39)		probably null	Het
Ikzf1	A	G	11: 11,711,359 (GRCm39)	S198G	probably benign	Het
Kcna4	T	A	2: 107,126,494 (GRCm39)	D409E	probably damaging	Het
Lipt2	GGCGCCCGCGCGCG	GGCG	7: 99,808,888 (GRCm39)		probably null	Het
Mgam	T	A	6: 40,705,977 (GRCm39)	D15E	probably benign	Het
Mmrn1	A	G	6: 60,964,193 (GRCm39)	D1065G	possibly damaging	Het
Plxnc1	A	C	10: 94,758,607 (GRCm39)	V363G	possibly damaging	Het
Ppp1r16b	G	A	2: 158,593,098 (GRCm39)	D226N	probably damaging	Het
Psg21	T	C	7: 18,384,678 (GRCm39)	Y356C	probably damaging	Het
Ranbp1	T	C	16: 18,059,659 (GRCm39)	D130G	probably damaging	Het
Rb1cc1	A	T	1: 6,310,451 (GRCm39)	Q283L	probably benign	Het
Rph3a	A	T	5: 121,097,365 (GRCm39)	H250Q	probably benign	Het
Secisbp2	G	A	13: 51,833,869 (GRCm39)	V670I	possibly damaging	Het
Skil	C	T	3: 31,172,686 (GRCm39)	A605V	probably damaging	Het
Slc28a3	T	C	13: 58,720,424 (GRCm39)	T312A	probably benign	Het
Ssh2	T	A	11: 77,344,843 (GRCm39)	C943S	probably benign	Het
Tgfbi	C	T	13: 56,779,894 (GRCm39)	A481V	probably damaging	Het
Tgfbr3	G	A	5: 107,297,750 (GRCm39)	P217S	probably damaging	Het
Tmem135	A	T	7: 88,954,721 (GRCm39)	W99R	probably benign	Het
Tmx1	A	G	12: 70,502,788 (GRCm39)	Y52C	possibly damaging	Het
Ube3b	A	T	5: 114,553,261 (GRCm39)		probably benign	Het
Vwa3b	A	G	1: 37,176,873 (GRCm39)	D40G		Het
Zfyve26	A	G	12: 79,311,083 (GRCm39)		probably benign	Het

Other mutations in Vmn2r57

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00159:Vmn2r57	APN	7	41,078,209 (GRCm39)	missense	probably benign
IGL01108:Vmn2r57	APN	7	41,077,008 (GRCm39)	missense	probably benign	0.01
IGL01112:Vmn2r57	APN	7	41,074,467 (GRCm39)	missense	probably damaging	1.00
IGL01516:Vmn2r57	APN	7	41,049,370 (GRCm39)	missense	probably damaging	1.00
IGL01880:Vmn2r57	APN	7	41,049,619 (GRCm39)	missense	possibly damaging	0.73
IGL02117:Vmn2r57	APN	7	41,049,874 (GRCm39)	missense	probably benign	0.00
IGL02500:Vmn2r57	APN	7	41,077,650 (GRCm39)	missense	probably benign
IGL02801:Vmn2r57	APN	7	41,098,056 (GRCm39)	missense	probably benign	0.13
IGL02993:Vmn2r57	APN	7	41,077,498 (GRCm39)	missense	probably benign	0.04
IGL02996:Vmn2r57	APN	7	41,049,165 (GRCm39)	missense	probably benign	0.02
R0008:Vmn2r57	UTSW	7	41,050,076 (GRCm39)	missense	probably damaging	1.00
R0032:Vmn2r57	UTSW	7	41,049,157 (GRCm39)	splice site	probably null
R0305:Vmn2r57	UTSW	7	41,076,967 (GRCm39)	missense	probably benign	0.00
R0469:Vmn2r57	UTSW	7	41,077,216 (GRCm39)	missense	possibly damaging	0.58
R0510:Vmn2r57	UTSW	7	41,077,216 (GRCm39)	missense	possibly damaging	0.58
R0847:Vmn2r57	UTSW	7	41,078,225 (GRCm39)	missense	probably benign	0.00
R1025:Vmn2r57	UTSW	7	41,077,228 (GRCm39)	missense	probably benign	0.24
R1081:Vmn2r57	UTSW	7	41,077,635 (GRCm39)	missense	possibly damaging	0.47
R1479:Vmn2r57	UTSW	7	41,077,254 (GRCm39)	missense	possibly damaging	0.45
R1579:Vmn2r57	UTSW	7	41,049,548 (GRCm39)	missense	probably benign	0.38
R1764:Vmn2r57	UTSW	7	41,050,067 (GRCm39)	missense	probably damaging	1.00
R1848:Vmn2r57	UTSW	7	41,077,531 (GRCm39)	missense	probably damaging	1.00
R2006:Vmn2r57	UTSW	7	41,098,001 (GRCm39)	missense	probably benign	0.00
R2197:Vmn2r57	UTSW	7	41,078,249 (GRCm39)	critical splice acceptor site	probably null
R2242:Vmn2r57	UTSW	7	41,077,498 (GRCm39)	missense	probably benign	0.00
R2394:Vmn2r57	UTSW	7	41,049,619 (GRCm39)	missense	possibly damaging	0.73
R3937:Vmn2r57	UTSW	7	41,077,554 (GRCm39)	missense	probably damaging	0.97
R4193:Vmn2r57	UTSW	7	41,077,663 (GRCm39)	missense	probably benign
R4423:Vmn2r57	UTSW	7	41,076,064 (GRCm39)	missense	probably damaging	1.00
R4865:Vmn2r57	UTSW	7	41,049,892 (GRCm39)	missense	probably damaging	1.00
R4947:Vmn2r57	UTSW	7	41,049,919 (GRCm39)	missense	probably damaging	1.00
R5042:Vmn2r57	UTSW	7	41,078,086 (GRCm39)	missense	probably benign	0.06
R5084:Vmn2r57	UTSW	7	41,075,974 (GRCm39)	critical splice donor site	probably null
R5177:Vmn2r57	UTSW	7	41,049,664 (GRCm39)	missense	probably benign	0.31
R5192:Vmn2r57	UTSW	7	41,077,363 (GRCm39)	missense	probably damaging	0.96
R5289:Vmn2r57	UTSW	7	41,049,398 (GRCm39)	missense	probably damaging	0.99
R5745:Vmn2r57	UTSW	7	41,097,895 (GRCm39)	missense	possibly damaging	0.51
R6051:Vmn2r57	UTSW	7	41,097,896 (GRCm39)	missense	probably benign	0.00
R6155:Vmn2r57	UTSW	7	41,078,114 (GRCm39)	missense	probably benign	0.14
R6248:Vmn2r57	UTSW	7	41,049,284 (GRCm39)	missense	probably benign
R6381:Vmn2r57	UTSW	7	41,078,242 (GRCm39)	missense	probably benign	0.08
R7019:Vmn2r57	UTSW	7	41,078,089 (GRCm39)	missense	probably damaging	1.00
R7126:Vmn2r57	UTSW	7	41,049,218 (GRCm39)	missense	possibly damaging	0.93
R7146:Vmn2r57	UTSW	7	41,097,895 (GRCm39)	missense	possibly damaging	0.51
R7215:Vmn2r57	UTSW	7	41,049,710 (GRCm39)	missense	probably benign	0.00
R7432:Vmn2r57	UTSW	7	41,076,148 (GRCm39)	missense	probably benign	0.01
R7633:Vmn2r57	UTSW	7	41,074,513 (GRCm39)	missense	possibly damaging	0.76
R7811:Vmn2r57	UTSW	7	41,074,439 (GRCm39)	nonsense	probably null
R8025:Vmn2r57	UTSW	7	41,076,183 (GRCm39)	missense	probably benign	0.00
R8332:Vmn2r57	UTSW	7	41,049,677 (GRCm39)	missense	probably benign	0.01
R8345:Vmn2r57	UTSW	7	41,076,968 (GRCm39)	missense	possibly damaging	0.81
R8360:Vmn2r57	UTSW	7	41,049,640 (GRCm39)	missense	probably damaging	1.00
R8738:Vmn2r57	UTSW	7	41,077,020 (GRCm39)	missense	probably benign	0.00
R8955:Vmn2r57	UTSW	7	41,049,571 (GRCm39)	missense	possibly damaging	0.64
R8985:Vmn2r57	UTSW	7	41,049,259 (GRCm39)	missense	probably benign
R9108:Vmn2r57	UTSW	7	41,078,192 (GRCm39)	missense	possibly damaging	0.87
R9160:Vmn2r57	UTSW	7	41,076,159 (GRCm39)	missense	possibly damaging	0.48
R9354:Vmn2r57	UTSW	7	41,049,663 (GRCm39)	missense	probably benign	0.01
R9566:Vmn2r57	UTSW	7	41,077,089 (GRCm39)	missense	probably benign	0.32
R9633:Vmn2r57	UTSW	7	41,076,006 (GRCm39)	missense	probably benign	0.00
X0026:Vmn2r57	UTSW	7	41,077,985 (GRCm39)	missense	possibly damaging	0.91
X0026:Vmn2r57	UTSW	7	41,077,549 (GRCm39)	missense	probably benign	0.03
X0065:Vmn2r57	UTSW	7	41,077,395 (GRCm39)	missense	probably benign	0.09
Z1176:Vmn2r57	UTSW	7	41,049,922 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- ATCCTACTGCTCACCTGTGG -3'
(R):5'- TTCTCCACTCAATATACTAAGAGTCCC -3'

Sequencing Primer
(F):5'- AGAGTTGTAGCAGTGTCCCAATATG -3'
(R):5'- GTCCCAGAGACCATGATTAATAAATG -3'

Posted On

2021-04-30