Mutagenetix > Incidental Mutation

Incidental Mutation 'R9563:Robo3'

721232

Institutional Source

Beutler Lab

Gene Symbol

Robo3

Ensembl Gene

ENSMUSG00000032128

Gene Name

roundabout guidance receptor 3

Synonyms

Robo3a, Rbig1, Rig1, Rig-1, Robo3b

MMRRC Submission

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9563 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

37327341-37344730 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 37340900 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 124 (F124S)

Ref Sequence

ENSEMBL: ENSMUSP00000110690 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034643] [ENSMUST00000115038] [ENSMUST00000170512]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000034643
AA Change: F102S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000034643
Gene: ENSMUSG00000032128
AA Change: F102S

Domain	Start	End	E-Value	Type
IGc2	54	128	9.7e-11	SMART
IGc2	156	221	1.44e-4	SMART
IGc2	248	311	1.89e-13	SMART
IGc2	337	409	9.84e-12	SMART
IGc2	441	506	2.09e-15	SMART
FN3	534	616	4.24e-14	SMART
FN3	648	731	3.06e0	SMART
FN3	747	832	1.97e-9	SMART
low complexity region	870	890	N/A	INTRINSIC
low complexity region	1055	1082	N/A	INTRINSIC
low complexity region	1131	1149	N/A	INTRINSIC
low complexity region	1155	1169	N/A	INTRINSIC
low complexity region	1193	1206	N/A	INTRINSIC
low complexity region	1245	1256	N/A	INTRINSIC
low complexity region	1268	1281	N/A	INTRINSIC
low complexity region	1336	1376	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000115038
AA Change: F124S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110690
Gene: ENSMUSG00000032128
AA Change: F124S

Domain	Start	End	E-Value	Type
low complexity region	34	47	N/A	INTRINSIC
IGc2	76	150	9.7e-11	SMART
IGc2	178	243	1.44e-4	SMART
IGc2	270	333	1.89e-13	SMART
IGc2	359	431	9.84e-12	SMART
IGc2	463	528	2.09e-15	SMART
FN3	556	638	4.24e-14	SMART
FN3	670	753	3.06e0	SMART
FN3	769	854	1.97e-9	SMART
low complexity region	892	912	N/A	INTRINSIC
low complexity region	1077	1104	N/A	INTRINSIC
low complexity region	1153	1171	N/A	INTRINSIC
low complexity region	1177	1191	N/A	INTRINSIC
low complexity region	1215	1228	N/A	INTRINSIC
low complexity region	1267	1278	N/A	INTRINSIC
low complexity region	1290	1303	N/A	INTRINSIC
low complexity region	1358	1398	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000170512
AA Change: F102S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. [provided by RefSeq, Dec 2009]
PHENOTYPE: Homozygous mutants display perinatal lethality, abnormal commissural axon growth, and fragile floor plates. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 127 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	T	A	6: 121,645,009 (GRCm39)	D953E	probably damaging	Het
Acod1	A	C	14: 103,287,109 (GRCm39)	Y80S	probably damaging	Het
Actr5	A	G	2: 158,470,135 (GRCm39)	D255G	probably damaging	Het
Akna	A	G	4: 63,312,944 (GRCm39)	V393A	probably damaging	Het
Amotl1	T	C	9: 14,473,513 (GRCm39)	K562R	possibly damaging	Het
Amz1	T	A	5: 140,738,133 (GRCm39)	D464E	probably damaging	Het
Anapc1	G	A	2: 128,505,980 (GRCm39)	Q619*	probably null	Het
Arfgef3	C	T	10: 18,522,275 (GRCm39)	G584R	probably damaging	Het
Armt1	AC	A	10: 4,400,848 (GRCm39)		probably null	Het
Atr	G	C	9: 95,802,833 (GRCm39)	V1832L	probably damaging	Het
Bsn	T	C	9: 107,984,616 (GRCm39)	D374G		Het
Cap1	A	G	4: 122,758,505 (GRCm39)	V225A	probably benign	Het
Cc2d1a	A	G	8: 84,863,758 (GRCm39)	S562P	probably benign	Het
Ccdc30	T	C	4: 119,250,821 (GRCm39)	N141S	possibly damaging	Het
Cdc42bpb	A	G	12: 111,265,762 (GRCm39)	V1390A	possibly damaging	Het
Cdc42ep1	A	T	15: 78,733,782 (GRCm39)	H294L	probably benign	Het
Col6a6	C	A	9: 105,572,952 (GRCm39)	G2185C	probably benign	Het
Col7a1	T	C	9: 108,791,809 (GRCm39)	V1232A	unknown	Het
Copb1	A	G	7: 113,836,034 (GRCm39)	I449T	possibly damaging	Het
Cplane1	T	G	15: 8,216,563 (GRCm39)	L594V	probably benign	Het
Ctdsp2	A	G	10: 126,832,040 (GRCm39)	D216G	probably damaging	Het
Cttnbp2	T	A	6: 18,367,382 (GRCm39)	K1645N	probably benign	Het
Cttnbp2	A	T	6: 18,427,467 (GRCm39)	L738*	probably null	Het
Cul9	T	A	17: 46,820,897 (GRCm39)	T1927S	probably benign	Het
Cyfip2	A	G	11: 46,151,707 (GRCm39)	C531R	probably benign	Het
Cyp2j6	A	G	4: 96,414,245 (GRCm39)	I340T	probably damaging	Het
Daam1	A	G	12: 71,992,251 (GRCm39)	E322G	unknown	Het
Dab2ip	T	A	2: 35,609,915 (GRCm39)	L710*	probably null	Het
Degs1l	G	T	1: 180,882,810 (GRCm39)	V191F	possibly damaging	Het
Dph1	T	C	11: 75,076,825 (GRCm39)	T60A	possibly damaging	Het
Eme1	T	C	11: 94,541,339 (GRCm39)	D161G	probably benign	Het
Epha3	C	T	16: 63,366,510 (GRCm39)	G980D	possibly damaging	Het
Eri3	T	A	4: 117,422,013 (GRCm39)	V136E	probably benign	Het
Espl1	T	A	15: 102,228,233 (GRCm39)	I1669N	possibly damaging	Het
Exoc6	T	C	19: 37,588,071 (GRCm39)	I568T	probably damaging	Het
F3	T	A	3: 121,527,822 (GRCm39)	L85H		Het
Fam186b	G	A	15: 99,177,616 (GRCm39)	A570V	probably damaging	Het
Fbxw14	T	C	9: 109,106,335 (GRCm39)	Y234C	probably benign	Het
Fbxw25	T	A	9: 109,483,676 (GRCm39)	N179Y		Het
Foxr1	T	A	9: 44,352,199 (GRCm39)		probably benign	Het
Gas8	T	A	8: 124,263,179 (GRCm39)	V452E	possibly damaging	Het
Gdpd4	T	C	7: 97,649,369 (GRCm39)	V450A	probably damaging	Het
Greb1	T	C	12: 16,774,824 (GRCm39)	Y191C	probably benign	Het
H3c11	T	C	13: 21,967,221 (GRCm39)	D107G	probably damaging	Het
Haus3	G	A	5: 34,325,300 (GRCm39)	R120W	probably benign	Het
Herc1	G	T	9: 66,294,193 (GRCm39)		probably null	Het
Hyal2	T	C	9: 107,447,844 (GRCm39)	W166R	probably damaging	Het
Inka1	T	A	9: 107,861,488 (GRCm39)	Y276F	probably benign	Het
Itga7	C	T	10: 128,789,669 (GRCm39)	A1003V	probably damaging	Het
Itga8	T	A	2: 12,165,219 (GRCm39)	I797F	possibly damaging	Het
Kank2	T	C	9: 21,705,852 (GRCm39)	T389A	possibly damaging	Het
Kcnb2	T	A	1: 15,779,737 (GRCm39)	I203N	probably damaging	Het
Kif21b	T	A	1: 136,077,166 (GRCm39)	L396Q	probably damaging	Het
Kng1	T	G	16: 22,879,170 (GRCm39)	I78S	probably damaging	Het
Ldlrad4	T	C	18: 68,387,551 (GRCm39)	S288P	probably benign	Het
Lmntd2	A	G	7: 140,790,701 (GRCm39)	S516P		Het
Lrp1b	T	A	2: 41,185,711 (GRCm39)	I1449F		Het
Lrrk2	T	A	15: 91,634,043 (GRCm39)	I1380K	possibly damaging	Het
Lvrn	T	G	18: 47,017,506 (GRCm39)	I612S	probably damaging	Het
Lyst	T	C	13: 13,812,408 (GRCm39)	L940P	probably benign	Het
Man2a2	T	A	7: 80,006,101 (GRCm39)	M996L	probably benign	Het
Map3k3	T	A	11: 106,041,860 (GRCm39)	I413N	probably damaging	Het
Map4k5	A	G	12: 69,863,167 (GRCm39)	V629A	probably benign	Het
Mapre2	A	G	18: 24,023,981 (GRCm39)	E325G	unknown	Het
Mbnl1	A	G	3: 60,520,715 (GRCm39)	N101S	probably benign	Het
Meltf	T	A	16: 31,703,869 (GRCm39)	C199S	probably damaging	Het
Mmab	T	C	5: 114,574,850 (GRCm39)	E141G	probably benign	Het
Mtcl2	G	A	2: 156,902,182 (GRCm39)	R278C	probably damaging	Het
Mtus2	T	C	5: 148,250,217 (GRCm39)	L1317P		Het
Muc6	T	A	7: 141,217,783 (GRCm39)	T2297S	possibly damaging	Het
Myom2	A	T	8: 15,158,399 (GRCm39)	K784*	probably null	Het
Nek1	A	G	8: 61,577,157 (GRCm39)	E1118G	probably benign	Het
Nrtn	A	G	17: 57,058,416 (GRCm39)	V195A	probably damaging	Het
Nsd3	G	A	8: 26,204,230 (GRCm39)	V420I		Het
Or52e4	T	C	7: 104,706,281 (GRCm39)	L276P	possibly damaging	Het
Or52z13	A	T	7: 103,247,225 (GRCm39)	Q234L	probably benign	Het
Or5ac23	T	C	16: 59,149,765 (GRCm39)	I36V	probably benign	Het
Or5b99	T	A	19: 12,976,983 (GRCm39)	I211N	probably damaging	Het
Or5t9	A	G	2: 86,659,098 (GRCm39)	M1V	probably null	Het
Or6ae1	G	A	7: 139,742,233 (GRCm39)	T210I	probably damaging	Het
Or6c33	T	C	10: 129,853,287 (GRCm39)	I19T	probably benign	Het
Or8b56	A	T	9: 38,739,014 (GRCm39)	H3L	probably benign	Het
Pde10a	G	T	17: 9,020,710 (GRCm39)	A216S	unknown	Het
Phldb2	T	C	16: 45,645,247 (GRCm39)	T400A	possibly damaging	Het
Pkd1l1	T	A	11: 8,815,502 (GRCm39)	T1920S		Het
Ptgs2	T	A	1: 149,981,419 (GRCm39)	N567K	probably benign	Het
Pwp1	T	A	10: 85,712,370 (GRCm39)	D141E	probably damaging	Het
Rab3ip	T	C	10: 116,754,668 (GRCm39)	E296G	probably null	Het
Rb1cc1	A	G	1: 6,314,339 (GRCm39)	K337R	probably benign	Het
Rbp3	A	G	14: 33,677,477 (GRCm39)	D475G	probably damaging	Het
Ric3	T	C	7: 108,637,997 (GRCm39)	E253G	possibly damaging	Het
Rictor	A	T	15: 6,797,562 (GRCm39)	N306I	possibly damaging	Het
Rnf214	A	T	9: 45,811,141 (GRCm39)	V174E	possibly damaging	Het
Rrad	A	G	8: 105,355,324 (GRCm39)	F228S	probably damaging	Het
Scn3a	A	T	2: 65,291,595 (GRCm39)	M1717K	probably damaging	Het
Scn3b	A	G	9: 40,193,729 (GRCm39)	D152G	probably benign	Het
Scn5a	C	A	9: 119,315,803 (GRCm39)	R1635L	probably damaging	Het
Sec61b	A	G	4: 47,483,049 (GRCm39)	Y93C	probably damaging	Het
Shprh	T	A	10: 11,042,235 (GRCm39)	Y739*	probably null	Het
Skor2	C	T	18: 76,946,376 (GRCm39)	H33Y	unknown	Het
Slc26a7	T	C	4: 14,519,496 (GRCm39)	N508S	probably benign	Het
Slc7a12	A	G	3: 14,564,360 (GRCm39)	I349V	possibly damaging	Het
Slit1	T	C	19: 41,596,874 (GRCm39)	Y1075C	probably damaging	Het
Sltm	C	T	9: 70,480,841 (GRCm39)	A231V	unknown	Het
Smg1	A	G	7: 117,812,208 (GRCm39)	S52P	unknown	Het
Sorl1	A	G	9: 41,957,893 (GRCm39)	Y584H	probably damaging	Het
Spmip2	A	T	3: 79,356,616 (GRCm39)	M140L	probably benign	Het
Ssbp1	T	A	6: 40,454,968 (GRCm39)	S142R	probably benign	Het
Stard3	A	G	11: 98,270,797 (GRCm39)		probably null	Het
Strn3	G	T	12: 51,674,300 (GRCm39)	H531N	possibly damaging	Het
Stx17	A	G	4: 48,180,739 (GRCm39)	T195A	probably damaging	Het
Tekt2	C	T	4: 126,217,444 (GRCm39)	R207H	probably damaging	Het
Tmed5	A	C	5: 108,280,100 (GRCm39)		probably null	Het
Tmem132a	C	T	19: 10,838,960 (GRCm39)	R494H	probably benign	Het
Tom1	A	G	8: 75,787,177 (GRCm39)	K406E	probably benign	Het
Trav5d-4	A	T	14: 53,239,473 (GRCm39)	N43Y	probably damaging	Het
Trbv19	G	T	6: 41,155,945 (GRCm39)	K105N	possibly damaging	Het
Trim34a	C	T	7: 103,910,328 (GRCm39)	Q377*	probably null	Het
Trp53bp2	T	A	1: 182,276,378 (GRCm39)	N786K	probably benign	Het
Trpc3	A	G	3: 36,705,683 (GRCm39)	M504T	probably benign	Het
Ttc28	A	T	5: 111,371,092 (GRCm39)	M545L	probably benign	Het
Uggt1	A	C	1: 36,204,627 (GRCm39)	V996G	possibly damaging	Het
Vmn2r65	G	A	7: 84,589,880 (GRCm39)	Q679*	probably null	Het
Wdfy4	T	C	14: 32,692,833 (GRCm39)	I2958V		Het
Zbtb37	C	T	1: 160,847,840 (GRCm39)	S389N	possibly damaging	Het
Zfp235	T	C	7: 23,841,669 (GRCm39)	V696A	possibly damaging	Het
Zzef1	T	A	11: 72,765,732 (GRCm39)	C1420S	probably damaging	Het

Other mutations in Robo3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00927:Robo3	APN	9	37,339,050 (GRCm39)	critical splice donor site	probably null
IGL01023:Robo3	APN	9	37,340,847 (GRCm39)	missense	probably damaging	1.00
IGL01431:Robo3	APN	9	37,330,407 (GRCm39)	unclassified	probably benign
IGL01993:Robo3	APN	9	37,335,949 (GRCm39)	missense	probably damaging	1.00
IGL02256:Robo3	APN	9	37,336,649 (GRCm39)	missense	probably damaging	1.00
IGL02323:Robo3	APN	9	37,333,497 (GRCm39)	missense	probably benign	0.05
IGL02561:Robo3	APN	9	37,338,387 (GRCm39)	missense	possibly damaging	0.84
IGL02866:Robo3	APN	9	37,333,602 (GRCm39)	missense	possibly damaging	0.89
IGL02897:Robo3	APN	9	37,338,798 (GRCm39)	nonsense	probably null
IGL03003:Robo3	APN	9	37,330,587 (GRCm39)	missense	probably damaging	1.00
IGL03307:Robo3	APN	9	37,333,860 (GRCm39)	missense	probably damaging	0.96
IGL03097:Robo3	UTSW	9	37,333,824 (GRCm39)	critical splice donor site	probably null
R0137:Robo3	UTSW	9	37,336,640 (GRCm39)	missense	probably benign	0.00
R0266:Robo3	UTSW	9	37,333,936 (GRCm39)	missense	probably damaging	0.96
R0390:Robo3	UTSW	9	37,333,473 (GRCm39)	missense	probably benign	0.00
R0505:Robo3	UTSW	9	37,328,055 (GRCm39)	unclassified	probably benign
R0815:Robo3	UTSW	9	37,333,479 (GRCm39)	missense	probably damaging	1.00
R0924:Robo3	UTSW	9	37,340,778 (GRCm39)	splice site	probably benign
R1167:Robo3	UTSW	9	37,335,203 (GRCm39)	nonsense	probably null
R1203:Robo3	UTSW	9	37,329,978 (GRCm39)	missense	probably damaging	1.00
R1451:Robo3	UTSW	9	37,329,007 (GRCm39)	missense	probably benign	0.01
R1575:Robo3	UTSW	9	37,340,957 (GRCm39)	missense	probably damaging	1.00
R1596:Robo3	UTSW	9	37,335,928 (GRCm39)	critical splice donor site	probably null
R1660:Robo3	UTSW	9	37,340,440 (GRCm39)	missense	probably damaging	1.00
R1677:Robo3	UTSW	9	37,329,005 (GRCm39)	missense	possibly damaging	0.75
R1839:Robo3	UTSW	9	37,333,623 (GRCm39)	missense	probably benign	0.00
R1878:Robo3	UTSW	9	37,333,461 (GRCm39)	missense	probably damaging	1.00
R1891:Robo3	UTSW	9	37,339,351 (GRCm39)	missense	probably damaging	1.00
R2040:Robo3	UTSW	9	37,338,760 (GRCm39)	missense	probably damaging	1.00
R2859:Robo3	UTSW	9	37,339,400 (GRCm39)	nonsense	probably null
R3786:Robo3	UTSW	9	37,333,521 (GRCm39)	missense	probably damaging	1.00
R3886:Robo3	UTSW	9	37,333,477 (GRCm39)	nonsense	probably null
R3888:Robo3	UTSW	9	37,333,477 (GRCm39)	nonsense	probably null
R3910:Robo3	UTSW	9	37,330,591 (GRCm39)	missense	probably damaging	1.00
R4212:Robo3	UTSW	9	37,333,194 (GRCm39)	missense	probably damaging	1.00
R4213:Robo3	UTSW	9	37,333,194 (GRCm39)	missense	probably damaging	1.00
R4691:Robo3	UTSW	9	37,336,514 (GRCm39)	missense	probably damaging	0.99
R4979:Robo3	UTSW	9	37,334,640 (GRCm39)	missense	probably damaging	1.00
R5238:Robo3	UTSW	9	37,328,175 (GRCm39)	missense	probably damaging	0.99
R5570:Robo3	UTSW	9	37,336,571 (GRCm39)	missense	possibly damaging	0.81
R5629:Robo3	UTSW	9	37,330,507 (GRCm39)	nonsense	probably null
R5770:Robo3	UTSW	9	37,330,497 (GRCm39)	missense	possibly damaging	0.87
R5837:Robo3	UTSW	9	37,341,112 (GRCm39)	critical splice acceptor site	probably null
R6021:Robo3	UTSW	9	37,333,829 (GRCm39)	nonsense	probably null
R6129:Robo3	UTSW	9	37,334,589 (GRCm39)	missense	probably benign
R6232:Robo3	UTSW	9	37,332,225 (GRCm39)	missense	probably damaging	1.00
R6233:Robo3	UTSW	9	37,332,225 (GRCm39)	missense	probably damaging	1.00
R6235:Robo3	UTSW	9	37,332,225 (GRCm39)	missense	probably damaging	1.00
R6326:Robo3	UTSW	9	37,338,323 (GRCm39)	missense	probably damaging	1.00
R6354:Robo3	UTSW	9	37,328,513 (GRCm39)	unclassified	probably benign
R6355:Robo3	UTSW	9	37,330,235 (GRCm39)	missense	possibly damaging	0.71
R6475:Robo3	UTSW	9	37,334,586 (GRCm39)	missense	probably damaging	0.99
R6937:Robo3	UTSW	9	37,341,176 (GRCm39)	missense	probably benign	0.16
R7201:Robo3	UTSW	9	37,335,626 (GRCm39)	nonsense	probably null
R7208:Robo3	UTSW	9	37,336,020 (GRCm39)	missense	probably damaging	0.99
R7249:Robo3	UTSW	9	37,336,129 (GRCm39)	missense	probably benign
R7376:Robo3	UTSW	9	37,344,212 (GRCm39)	missense	probably damaging	1.00
R7380:Robo3	UTSW	9	37,329,852 (GRCm39)	missense	probably damaging	1.00
R7448:Robo3	UTSW	9	37,336,111 (GRCm39)	missense	possibly damaging	0.89
R7475:Robo3	UTSW	9	37,336,674 (GRCm39)	missense	probably benign	0.01
R7496:Robo3	UTSW	9	37,339,121 (GRCm39)	missense	probably damaging	1.00
R7587:Robo3	UTSW	9	37,340,942 (GRCm39)	missense	probably damaging	1.00
R7694:Robo3	UTSW	9	37,329,816 (GRCm39)	missense	probably benign	0.14
R8381:Robo3	UTSW	9	37,341,056 (GRCm39)	missense	probably damaging	1.00
R8464:Robo3	UTSW	9	37,332,726 (GRCm39)	missense	probably damaging	1.00
R8495:Robo3	UTSW	9	37,336,664 (GRCm39)	missense	probably damaging	1.00
R8886:Robo3	UTSW	9	37,328,768 (GRCm39)	missense	probably damaging	0.99
R9422:Robo3	UTSW	9	37,329,789 (GRCm39)	missense	probably benign	0.03
R9564:Robo3	UTSW	9	37,340,900 (GRCm39)	missense	probably damaging	1.00
R9681:Robo3	UTSW	9	37,339,087 (GRCm39)	missense	probably benign	0.45
R9681:Robo3	UTSW	9	37,334,558 (GRCm39)	missense	possibly damaging	0.75
X0024:Robo3	UTSW	9	37,339,151 (GRCm39)	missense	probably damaging	1.00
X0027:Robo3	UTSW	9	37,339,121 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGACGTTCATTGGGCTGACC -3'
(R):5'- TCTCCAGGGTCAAGAGTTGG -3'

Sequencing Primer
(F):5'- CCCCAGGCCAACTTTCTG -3'
(R):5'- TTGGGCCAGAAGATGCCATG -3'

Posted On

2022-08-09