Incidental Mutation 'R4755:Olfr653'
ID 357919
Institutional Source Beutler Lab
Gene Symbol Olfr653
Ensembl Gene ENSMUSG00000073926
Gene Name olfactory receptor 653
Synonyms MOR33-1, GA_x6K02T2PBJ9-7206970-7207923
MMRRC Submission 042033-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.063) question?
Stock # R4755 (G1)
Quality Score 225
Status Validated
Chromosome 7
Chromosomal Location 104577314-104584117 bp(+) (GRCm38)
Type of Mutation nonsense
DNA Base Change (assembly) T to A at 104580061 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Stop codon at position 138 (Y138*)
Ref Sequence ENSEMBL: ENSMUSP00000149192 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000098174] [ENSMUST00000215585] [ENSMUST00000217466]
AlphaFold E9PVA0
Predicted Effect probably null
Transcript: ENSMUST00000098174
AA Change: Y138*
SMART Domains Protein: ENSMUSP00000095776
Gene: ENSMUSG00000073926
AA Change: Y138*

Pfam:7tm_4 37 316 2.2e-111 PFAM
Pfam:7TM_GPCR_Srsx 41 179 4.6e-10 PFAM
Pfam:7tm_1 47 298 5.9e-22 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000215585
AA Change: Y138*
Predicted Effect probably null
Transcript: ENSMUST00000217466
AA Change: Y138*
Meta Mutation Damage Score 0.9755 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.7%
  • 10x: 97.3%
  • 20x: 95.3%
Validation Efficiency 96% (112/117)
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 113 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930449A18Rik T A 3: 59,825,859 (GRCm38) noncoding transcript Het
Accs T C 2: 93,841,337 (GRCm38) E236G probably damaging Het
Agrn C T 4: 156,173,522 (GRCm38) probably benign Het
Ahi1 A G 10: 21,055,047 (GRCm38) I929V possibly damaging Het
Akap5 C A 12: 76,327,807 (GRCm38) C4* probably null Het
Amotl2 A T 9: 102,720,480 (GRCm38) H146L probably damaging Het
Ank1 A G 8: 23,104,974 (GRCm38) N666S probably damaging Het
Atp10d A T 5: 72,246,166 (GRCm38) T373S probably benign Het
Bpifb9b T A 2: 154,319,694 (GRCm38) M582K probably benign Het
Brca2 T A 5: 150,559,987 (GRCm38) probably null Het
C130079G13Rik C T 3: 59,936,314 (GRCm38) A143V probably benign Het
C330021F23Rik A T 8: 3,583,922 (GRCm38) S8C probably damaging Het
Ccdc149 A G 5: 52,404,151 (GRCm38) V229A probably damaging Het
Cdk5rap2 A G 4: 70,238,425 (GRCm38) S1617P probably damaging Het
Cenpk A T 13: 104,230,871 (GRCm38) M37L probably benign Het
Cenpk A T 13: 104,249,512 (GRCm38) H305L probably benign Het
Ces5a C A 8: 93,535,677 (GRCm38) A11S probably benign Het
Cfap65 T C 1: 74,928,361 (GRCm38) E186G probably damaging Het
Cfh T A 1: 140,088,808 (GRCm38) I593F probably damaging Het
Clstn2 C T 9: 97,445,673 (GRCm38) V961I probably benign Het
Cog5 T A 12: 31,869,406 (GRCm38) probably null Het
Col4a4 T C 1: 82,541,174 (GRCm38) D100G unknown Het
Cyp3a41a T C 5: 145,715,506 (GRCm38) D61G probably damaging Het
Dnah10 A G 5: 124,747,745 (GRCm38) N655S probably benign Het
Dnaic1 C G 4: 41,610,269 (GRCm38) T295R probably damaging Het
Dnajc6 C T 4: 101,550,799 (GRCm38) A44V probably damaging Het
Eif4enif1 A T 11: 3,244,016 (GRCm38) D960V probably damaging Het
Fam167b C T 4: 129,578,342 (GRCm38) G12R probably damaging Het
Fam20b A T 1: 156,687,496 (GRCm38) Y266* probably null Het
Fer1l6 T A 15: 58,640,211 (GRCm38) V1509D probably benign Het
Fhad1 A T 4: 141,928,483 (GRCm38) I105N probably damaging Het
Fmo2 T C 1: 162,888,805 (GRCm38) D71G probably damaging Het
Folr2 T C 7: 101,843,799 (GRCm38) T6A possibly damaging Het
Fry A C 5: 150,398,254 (GRCm38) E1018A probably damaging Het
Gas2l2 A G 11: 83,429,367 (GRCm38) I21T probably damaging Het
Gfra1 T C 19: 58,453,244 (GRCm38) Y85C probably damaging Het
Gm9117 T C 3: 93,938,786 (GRCm38) probably null Het
Gpld1 A T 13: 24,979,688 (GRCm38) Y43F probably benign Het
Gpld1 T A 13: 24,979,692 (GRCm38) Y44* probably null Het
Grid2 A G 6: 63,908,988 (GRCm38) T123A probably benign Het
Grina T C 15: 76,249,242 (GRCm38) L305P probably damaging Het
Gucy1a1 G A 3: 82,094,795 (GRCm38) A659V probably benign Het
H2-T10 T A 17: 36,118,945 (GRCm38) K319* probably null Het
Hey2 A T 10: 30,834,304 (GRCm38) V151E probably benign Het
Ighv1-69 G A 12: 115,623,558 (GRCm38) T13I probably benign Het
Il1rap C A 16: 26,722,782 (GRCm38) A591E probably benign Het
Ildr1 T C 16: 36,722,021 (GRCm38) L261P probably benign Het
Jak1 A G 4: 101,174,157 (GRCm38) Y463H probably damaging Het
Lrp1b T A 2: 41,471,016 (GRCm38) T592S probably benign Het
Lrp1b T C 2: 41,269,273 (GRCm38) I1666V probably benign Het
Lrrc36 A G 8: 105,452,144 (GRCm38) T445A possibly damaging Het
Ly9 G A 1: 171,607,238 (GRCm38) S29F probably damaging Het
Mapk7 A C 11: 61,490,843 (GRCm38) C32W probably damaging Het
March10 C T 11: 105,364,476 (GRCm38) probably benign Het
Mier2 A T 10: 79,549,197 (GRCm38) M119K probably damaging Het
Mpv17 A T 5: 31,145,982 (GRCm38) C59* probably null Het
Mrpl27 G A 11: 94,653,833 (GRCm38) probably benign Het
Myo18b G A 5: 112,874,474 (GRCm38) Q351* probably null Het
Myo1a A G 10: 127,715,688 (GRCm38) I704M probably damaging Het
Nadsyn1 A G 7: 143,806,913 (GRCm38) C373R probably damaging Het
Nckipsd C A 9: 108,814,739 (GRCm38) A513E probably benign Het
Neb T C 2: 52,220,209 (GRCm38) D209G probably damaging Het
Nkapl T A 13: 21,468,287 (GRCm38) Q52L unknown Het
Nptx2 G A 5: 144,546,440 (GRCm38) S126N probably benign Het
Olfr13 G A 6: 43,174,043 (GRCm38) S19N probably benign Het
Olfr829 A T 9: 18,857,180 (GRCm38) H185L probably benign Het
Olfr917 A G 9: 38,665,832 (GRCm38) V4A probably benign Het
Pclo A T 5: 14,714,348 (GRCm38) R4278S unknown Het
Pcnx T G 12: 81,950,294 (GRCm38) L988R probably damaging Het
Prl2c5 C A 13: 13,189,385 (GRCm38) N75K probably benign Het
Prpf19 T G 19: 10,897,790 (GRCm38) probably benign Het
Ralgapa1 A G 12: 55,712,748 (GRCm38) S997P probably damaging Het
Rangap1 T C 15: 81,712,917 (GRCm38) T226A probably benign Het
Rimklb G A 6: 122,456,406 (GRCm38) L262F probably damaging Het
Rnf169 A G 7: 99,925,723 (GRCm38) M555T probably benign Het
Rp1l1 A T 14: 64,030,070 (GRCm38) D1035V probably benign Het
Scd2 T A 19: 44,301,352 (GRCm38) L262Q probably damaging Het
Scgb2b12 T A 7: 32,325,531 (GRCm38) M84L probably benign Het
Shroom3 G T 5: 92,943,086 (GRCm38) V1151F probably damaging Het
Sipa1l1 G A 12: 82,372,386 (GRCm38) V613I possibly damaging Het
Slc25a23 T A 17: 57,052,794 (GRCm38) D67V possibly damaging Het
Slc25a39 C T 11: 102,406,666 (GRCm38) probably benign Het
Slc4a10 T A 2: 62,296,988 (GRCm38) F895Y probably damaging Het
Slc5a4a A C 10: 76,186,564 (GRCm38) K578Q probably benign Het
Slc6a13 G A 6: 121,325,049 (GRCm38) G197S probably damaging Het
Smarca2 A T 19: 26,654,483 (GRCm38) E566V possibly damaging Het
Sorbs3 A T 14: 70,184,099 (GRCm38) N594K probably benign Het
Spata22 A T 11: 73,345,756 (GRCm38) D296V probably damaging Het
Sphk2 G A 7: 45,713,634 (GRCm38) A11V possibly damaging Het
Spp1 A T 5: 104,435,215 (GRCm38) probably benign Het
Strn3 T A 12: 51,610,216 (GRCm38) I760L possibly damaging Het
Syk A T 13: 52,641,986 (GRCm38) Y539F probably benign Het
Thsd7b T A 1: 130,210,264 (GRCm38) Y1560N probably benign Het
Tmod2 C A 9: 75,597,212 (GRCm38) E42* probably null Het
Tom1l1 T C 11: 90,685,116 (GRCm38) E30G probably damaging Het
Trav10 G A 14: 53,506,061 (GRCm38) A40T probably benign Het
Trav14-2 G A 14: 53,640,780 (GRCm38) probably benign Het
Tril T A 6: 53,818,464 (GRCm38) E591V probably damaging Het
Trp53bp1 T A 2: 121,228,606 (GRCm38) R179* probably null Het
Tspoap1 A T 11: 87,771,663 (GRCm38) D562V possibly damaging Het
Usp44 A T 10: 93,846,906 (GRCm38) H406L probably damaging Het
Vangl1 A G 3: 102,158,292 (GRCm38) I509T probably benign Het
Vax2 T G 6: 83,711,397 (GRCm38) L34W probably damaging Het
Vmn1r55 A T 7: 5,147,026 (GRCm38) C133S probably damaging Het
Vmn2r8 T A 5: 108,801,700 (GRCm38) D427V probably benign Het
Vwde A G 6: 13,205,852 (GRCm38) I232T possibly damaging Het
Wnk1 C A 6: 119,963,470 (GRCm38) A769S probably damaging Het
Xpo4 A C 14: 57,618,181 (GRCm38) S264A probably benign Het
Zfp330 A T 8: 82,769,386 (GRCm38) C75* probably null Het
Zfp526 T A 7: 25,225,639 (GRCm38) L441Q probably benign Het
Zfp607b T G 7: 27,703,505 (GRCm38) L462R probably damaging Het
Zfp719 T A 7: 43,590,793 (GRCm38) F602I probably damaging Het
Other mutations in Olfr653
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01588:Olfr653 APN 7 104,579,943 (GRCm38) missense probably damaging 1.00
I2288:Olfr653 UTSW 7 104,580,386 (GRCm38) missense probably damaging 1.00
PIT4131001:Olfr653 UTSW 7 104,580,030 (GRCm38) missense probably damaging 0.99
R0325:Olfr653 UTSW 7 104,580,360 (GRCm38) missense probably damaging 1.00
R1579:Olfr653 UTSW 7 104,580,061 (GRCm38) nonsense probably null
R1599:Olfr653 UTSW 7 104,579,648 (GRCm38) start codon destroyed probably null
R1956:Olfr653 UTSW 7 104,579,909 (GRCm38) missense probably damaging 1.00
R2134:Olfr653 UTSW 7 104,579,641 (GRCm38) start gained probably benign
R2418:Olfr653 UTSW 7 104,579,934 (GRCm38) missense probably benign 0.00
R2483:Olfr653 UTSW 7 104,579,942 (GRCm38) missense probably damaging 1.00
R3623:Olfr653 UTSW 7 104,579,942 (GRCm38) missense probably damaging 1.00
R4049:Olfr653 UTSW 7 104,580,368 (GRCm38) missense probably benign
R4757:Olfr653 UTSW 7 104,580,197 (GRCm38) missense possibly damaging 0.95
R4828:Olfr653 UTSW 7 104,579,973 (GRCm38) missense possibly damaging 0.62
R4842:Olfr653 UTSW 7 104,580,215 (GRCm38) missense probably benign 0.45
R5496:Olfr653 UTSW 7 104,580,494 (GRCm38) missense probably damaging 1.00
R6830:Olfr653 UTSW 7 104,580,240 (GRCm38) missense probably damaging 1.00
R7320:Olfr653 UTSW 7 104,580,438 (GRCm38) nonsense probably null
R7590:Olfr653 UTSW 7 104,579,942 (GRCm38) missense probably damaging 1.00
R7968:Olfr653 UTSW 7 104,580,388 (GRCm38) nonsense probably null
R8491:Olfr653 UTSW 7 104,580,035 (GRCm38) missense probably damaging 0.99
R9306:Olfr653 UTSW 7 104,579,921 (GRCm38) missense probably damaging 1.00
R9521:Olfr653 UTSW 7 104,579,648 (GRCm38) start codon destroyed probably null
R9557:Olfr653 UTSW 7 104,580,561 (GRCm38) missense probably damaging 1.00
R9682:Olfr653 UTSW 7 104,580,227 (GRCm38) missense probably damaging 1.00
RF020:Olfr653 UTSW 7 104,580,290 (GRCm38) missense probably benign 0.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2015-11-11