Mutagenetix > Incidental Mutation

Incidental Mutation 'R5125:Zfp976'

393499

Institutional Source

Beutler Lab

Gene Symbol

Zfp976

Ensembl Gene

ENSMUSG00000074158

Gene Name

zinc finger protein 976

Synonyms

9830147E19Rik

MMRRC Submission

042713-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.102) question?

Stock #

R5125 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

42609526-42642588 bp(-) (GRCm38)

Type of Mutation

splice site (3 bp from exon)

DNA Base Change (assembly)

A to G at 42612501 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000141023 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098503] [ENSMUST00000187616]

AlphaFold

E9Q981

Predicted Effect

probably null
Transcript: ENSMUST00000098503

SMART Domains

Protein: ENSMUSP00000096105
Gene: ENSMUSG00000074158

Domain	Start	End	E-Value	Type
KRAB	4	66	1.73e-18	SMART
ZnF_C2H2	131	153	7.78e-3	SMART
ZnF_C2H2	159	181	1.67e-2	SMART
ZnF_C2H2	187	209	5.9e-3	SMART
ZnF_C2H2	215	237	1.38e-3	SMART
ZnF_C2H2	243	265	8.94e-3	SMART
ZnF_C2H2	271	293	2.24e-3	SMART
ZnF_C2H2	299	321	1.03e-2	SMART
ZnF_C2H2	327	349	1.58e-3	SMART
ZnF_C2H2	355	377	7.9e-4	SMART
ZnF_C2H2	383	405	5.9e-3	SMART
ZnF_C2H2	411	433	2.57e-3	SMART
ZnF_C2H2	439	461	3.16e-3	SMART
ZnF_C2H2	467	489	1.26e-2	SMART
ZnF_C2H2	495	517	8.34e-3	SMART
ZnF_C2H2	523	545	3.63e-3	SMART
ZnF_C2H2	551	573	4.79e-3	SMART
ZnF_C2H2	579	601	9.73e-4	SMART
ZnF_C2H2	607	629	3.63e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000107994

Predicted Effect

probably null
Transcript: ENSMUST00000187616

SMART Domains

Protein: ENSMUSP00000141023
Gene: ENSMUSG00000074158

Domain	Start	End	E-Value	Type
KRAB	3	65	7.4e-21	SMART
ZnF_C2H2	130	152	3.2e-5	SMART
ZnF_C2H2	158	180	7.1e-5	SMART
ZnF_C2H2	186	208	2.4e-5	SMART
ZnF_C2H2	214	236	5.7e-6	SMART
ZnF_C2H2	242	264	3.8e-5	SMART
ZnF_C2H2	270	292	9.7e-6	SMART
ZnF_C2H2	298	320	4.2e-5	SMART
ZnF_C2H2	326	348	6.6e-6	SMART
ZnF_C2H2	354	376	3.3e-6	SMART
ZnF_C2H2	382	404	2.5e-5	SMART
ZnF_C2H2	410	432	1e-5	SMART
ZnF_C2H2	438	460	1.3e-5	SMART
ZnF_C2H2	466	488	5.2e-5	SMART
ZnF_C2H2	494	516	3.6e-5	SMART
ZnF_C2H2	522	544	1.6e-5	SMART
ZnF_C2H2	550	572	2e-5	SMART
ZnF_C2H2	578	600	4e-6	SMART
ZnF_C2H2	606	628	1.5e-5	SMART

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.1%
20x: 91.9%

Validation Efficiency

96% (49/51)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abt1	G	T	13: 23,422,649	A94E	possibly damaging	Het
Carmil2	G	T	8: 105,696,889	G1207V	probably damaging	Het
Cyp2c66	A	G	19: 39,171,029	Y308C	probably damaging	Het
Dcc	A	G	18: 71,456,877	F683L	probably benign	Het
Denr	A	T	5: 123,927,081	I166F	probably damaging	Het
Dhx29	T	G	13: 112,932,600	S155A	possibly damaging	Het
Dsg1b	G	A	18: 20,397,503	G405E	probably damaging	Het
Ei24	T	A	9: 36,782,446		probably benign	Het
Exo5	A	T	4: 120,921,537		probably null	Het
Gm15922	T	A	7: 3,739,397	K44*	probably null	Het
Gm7356	T	C	17: 14,001,314	D151G	probably damaging	Het
Grin1	T	C	2: 25,296,827		probably benign	Het
Grin2b	C	T	6: 135,923,299	V195M	possibly damaging	Het
Hephl1	T	A	9: 15,086,172	K399N	probably damaging	Het
Itgb4	C	T	11: 115,984,157	R447W	probably benign	Het
Kmt2c	A	G	5: 25,284,381	V4520A	probably damaging	Het
Lair1	G	A	7: 4,010,489	T82I	possibly damaging	Het
Lmx1a	C	T	1: 167,830,687	S213L	possibly damaging	Het
Ly6g6d	A	G	17: 35,074,442	I8T	possibly damaging	Het
Mcm4	T	A	16: 15,635,303	D174V	probably benign	Het
Mcph1	T	A	8: 18,607,326	D60E	probably damaging	Het
Med12l	G	A	3: 59,267,214	G1851D	possibly damaging	Het
Olfr156	T	A	4: 43,820,480	I294F	probably benign	Het
Olfr868	T	A	9: 20,101,192	C144*	probably null	Het
P2rx2	G	A	5: 110,342,651	T66I	possibly damaging	Het
Pcdh15	A	G	10: 74,584,080	E1197G	probably damaging	Het
Ppwd1	A	T	13: 104,220,435	S191T	probably benign	Het
Rbm39	A	T	2: 156,162,865	M184K	probably damaging	Het
Reln	A	G	5: 21,913,241	V2935A	possibly damaging	Het
Robo4	T	C	9: 37,407,960	W535R	probably damaging	Het
Rsf1	A	G	7: 97,661,872	D603G	possibly damaging	Het
Sh3rf3	C	G	10: 59,131,190	P785A	probably benign	Het
Slc22a26	T	C	19: 7,790,175	T289A	possibly damaging	Het
Slc24a3	T	C	2: 145,518,847	V120A	possibly damaging	Het
Sost	C	T	11: 101,963,941	G181R	probably damaging	Het
Sp2	A	G	11: 96,955,838	F554L	probably benign	Het
Stim2	T	C	5: 54,110,597	S87P	probably damaging	Het
Tcrg-C4	A	G	13: 19,344,762		probably benign	Het
Tecta	T	C	9: 42,375,185	D725G	probably damaging	Het
Ube4b	T	C	4: 149,342,992	M900V	probably damaging	Het
Ugt2b38	A	G	5: 87,411,812	M407T	probably damaging	Het
Vmn1r87	G	T	7: 13,131,865	A165E	possibly damaging	Het
Zc3h6	A	G	2: 129,014,479	H493R	possibly damaging	Het
Zfhx2	A	T	14: 55,074,775	F154Y	probably benign	Het
Zfp729a	A	G	13: 67,637,645		probably null	Het
Zhx1	C	G	15: 58,054,074	G259R	probably damaging	Het
Znfx1	A	T	2: 167,046,939	V783E	possibly damaging	Het

Other mutations in Zfp976

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Zfp976	APN	7	42613685	missense	unknown
IGL01102:Zfp976	APN	7	42613909	nonsense	probably null
IGL01111:Zfp976	APN	7	42616287	missense	probably damaging	0.99
IGL01628:Zfp976	APN	7	42612511	missense	unknown
IGL02008:Zfp976	APN	7	42614232	splice site	probably benign
IGL02548:Zfp976	APN	7	42612529	missense	unknown
R0190:Zfp976	UTSW	7	42642524	start gained	probably benign
R0685:Zfp976	UTSW	7	42613717	missense	probably damaging	0.98
R1310:Zfp976	UTSW	7	42613186	missense	probably damaging	1.00
R1353:Zfp976	UTSW	7	42616018	missense	probably damaging	0.99
R1447:Zfp976	UTSW	7	42612599	missense	possibly damaging	0.79
R1569:Zfp976	UTSW	7	42613382	missense	probably damaging	1.00
R1702:Zfp976	UTSW	7	42616000	missense	possibly damaging	0.86
R1829:Zfp976	UTSW	7	42616311	missense	probably damaging	1.00
R1939:Zfp976	UTSW	7	42613681	missense	unknown
R1978:Zfp976	UTSW	7	42613841	missense	probably damaging	1.00
R1981:Zfp976	UTSW	7	42613622	missense	probably damaging	0.99
R2160:Zfp976	UTSW	7	42613930	missense	probably benign
R2192:Zfp976	UTSW	7	42613271	missense	probably damaging	1.00
R3121:Zfp976	UTSW	7	42613514	missense	probably damaging	1.00
R4210:Zfp976	UTSW	7	42616325	missense	probably damaging	0.99
R4724:Zfp976	UTSW	7	42613033	missense	possibly damaging	0.91
R4943:Zfp976	UTSW	7	42612422	unclassified	probably benign
R5047:Zfp976	UTSW	7	42613419	nonsense	probably null
R5071:Zfp976	UTSW	7	42612930	nonsense	probably null
R5178:Zfp976	UTSW	7	42612501	splice site	probably null
R5305:Zfp976	UTSW	7	42613478	missense	probably benign	0.00
R5777:Zfp976	UTSW	7	42614080	missense	probably benign	0.00
R6153:Zfp976	UTSW	7	42614186	missense	probably damaging	0.99
R6694:Zfp976	UTSW	7	42614186	missense	probably damaging	0.99
R7226:Zfp976	UTSW	7	42613260	nonsense	probably null
R7479:Zfp976	UTSW	7	42613179	missense	probably benign	0.01
R7561:Zfp976	UTSW	7	42616277	missense	probably damaging	1.00
R8178:Zfp976	UTSW	7	42613535	missense	probably benign	0.03
R8261:Zfp976	UTSW	7	42612701	missense	unknown
R8715:Zfp976	UTSW	7	42613445	missense	possibly damaging	0.89
R8921:Zfp976	UTSW	7	42613151	missense	possibly damaging	0.57
R9168:Zfp976	UTSW	7	42613587	nonsense	probably null
R9575:Zfp976	UTSW	7	42612617	missense	unknown
Z1088:Zfp976	UTSW	7	42612760	missense	possibly damaging	0.71

Predicted Primers

PCR Primer
(F):5'- ATGTATTCGAAAACACCTGGGC -3'
(R):5'- CTTCGAATGCATGAAAGAACACATA -3'

Sequencing Primer
(F):5'- CCTGGGCTGTGAAAAAGCTTTATCAC -3'
(R):5'- CCAGCTATCTTCAAATGCATGAAAG -3'

Posted On

2016-06-15