Mutagenetix > Incidental Mutation

Incidental Mutation 'R6287:Vmn2r87'

508274

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r87

Ensembl Gene

ENSMUSG00000091511

Gene Name

vomeronasal 2, receptor 87

Synonyms

EG625131

MMRRC Submission

044457-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R6287 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

130307690-130333248 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (1 bp from exon)

DNA Base Change (assembly)

C to T at 130314291 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000129215 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164227]

AlphaFold

E9PZX4

Predicted Effect

probably null
Transcript: ENSMUST00000164227

SMART Domains

Protein: ENSMUSP00000129215
Gene: ENSMUSG00000091511

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	422	1.8e-27	PFAM
Pfam:NCD3G	508	562	1.8e-19	PFAM
Pfam:7tm_3	595	829	8.8e-55	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 96.3%

Validation Efficiency

94% (34/36)

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy7	A	G	8: 89,037,736 (GRCm39)	N268S	possibly damaging	Het
Ahnak	T	A	19: 8,992,367 (GRCm39)	H4550Q	probably benign	Het
C330018D20Rik	A	C	18: 57,095,407 (GRCm39)		probably null	Het
Camta2	G	C	11: 70,572,295 (GRCm39)	Q310E	probably damaging	Het
Caskin1	A	T	17: 24,715,683 (GRCm39)	K149M	probably damaging	Het
Chml	T	C	1: 175,514,569 (GRCm39)	K108E	probably benign	Het
Ddx60	T	C	8: 62,403,612 (GRCm39)	L345P	probably damaging	Het
Defb6	T	A	8: 19,278,085 (GRCm39)	C52*	probably null	Het
Flii	A	T	11: 60,612,423 (GRCm39)	I288N	probably damaging	Het
Fmnl2	T	C	2: 52,904,860 (GRCm39)	Y55H	probably damaging	Het
Galk2	A	T	2: 125,712,268 (GRCm39)		probably benign	Het
Ift140	T	A	17: 25,269,408 (GRCm39)	C688S	probably benign	Het
Lmln	T	C	16: 32,894,555 (GRCm39)		probably null	Het
Muc16	T	C	9: 18,570,330 (GRCm39)	T730A	unknown	Het
Nup88	T	C	11: 70,856,581 (GRCm39)	E184G	probably benign	Het
Omt2b	T	C	9: 78,235,543 (GRCm39)	F27L	possibly damaging	Het
Or10g3	A	T	14: 52,609,748 (GRCm39)	V254E	probably damaging	Het
Or2a7	A	G	6: 43,151,369 (GRCm39)	I150V	probably benign	Het
Or2y13	A	G	11: 49,415,072 (GRCm39)	N174S	probably damaging	Het
Or4a15	G	A	2: 89,193,363 (GRCm39)	R137*	probably null	Het
Or6c69b	T	C	10: 129,627,254 (GRCm39)	E68G	probably damaging	Het
Peg10	T	TCCC	6: 4,756,451 (GRCm39)		probably benign	Het
Pigf	G	A	17: 87,304,967 (GRCm39)	A192V	probably damaging	Het
Pikfyve	T	C	1: 65,292,691 (GRCm39)		probably null	Het
Ppat	A	T	5: 77,066,061 (GRCm39)	Y352*	probably null	Het
Rapgef6	T	G	11: 54,517,164 (GRCm39)		probably null	Het
Rrp15	A	T	1: 186,481,373 (GRCm39)	S45T	probably benign	Het
Sap18	A	G	14: 58,035,771 (GRCm39)	E30G	probably damaging	Het
Snx6	G	T	12: 54,793,813 (GRCm39)	A284E	possibly damaging	Het
Tm4sf4	T	A	3: 57,333,112 (GRCm39)	I26N	probably damaging	Het
Traf5	A	G	1: 191,731,833 (GRCm39)	L336P	probably damaging	Het
Ttl	G	A	2: 128,931,041 (GRCm39)	A335T	probably damaging	Het
Zfp74	G	A	7: 29,635,201 (GRCm39)	T169I	probably benign	Het
Zfp937	A	T	2: 150,080,261 (GRCm39)	H97L	possibly damaging	Het
Zzef1	G	T	11: 72,813,938 (GRCm39)	E2842D	probably damaging	Het

Other mutations in Vmn2r87

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01090:Vmn2r87	APN	10	130,333,247 (GRCm39)	start codon destroyed	probably null	1.00
IGL01295:Vmn2r87	APN	10	130,307,878 (GRCm39)	missense	probably damaging	1.00
IGL01411:Vmn2r87	APN	10	130,308,429 (GRCm39)	missense	probably benign	0.03
IGL01680:Vmn2r87	APN	10	130,315,586 (GRCm39)	nonsense	probably null
IGL01822:Vmn2r87	APN	10	130,307,991 (GRCm39)	missense	probably damaging	1.00
IGL01835:Vmn2r87	APN	10	130,314,978 (GRCm39)	missense	probably damaging	1.00
IGL01965:Vmn2r87	APN	10	130,314,924 (GRCm39)	missense	possibly damaging	0.49
IGL02562:Vmn2r87	APN	10	130,314,513 (GRCm39)	missense	probably damaging	1.00
IGL02665:Vmn2r87	APN	10	130,333,049 (GRCm39)	missense	probably benign	0.16
IGL03202:Vmn2r87	APN	10	130,333,091 (GRCm39)	missense	probably benign
FR4304:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4340:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4342:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4589:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
LCD18:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
R0344:Vmn2r87	UTSW	10	130,315,806 (GRCm39)	missense	probably damaging	1.00
R0374:Vmn2r87	UTSW	10	130,307,848 (GRCm39)	missense	probably damaging	1.00
R0384:Vmn2r87	UTSW	10	130,307,712 (GRCm39)	missense	probably benign
R1144:Vmn2r87	UTSW	10	130,312,098 (GRCm39)	splice site	probably benign
R1172:Vmn2r87	UTSW	10	130,313,453 (GRCm39)	missense	probably benign	0.03
R1860:Vmn2r87	UTSW	10	130,315,755 (GRCm39)	missense	probably benign	0.00
R1866:Vmn2r87	UTSW	10	130,308,441 (GRCm39)	missense	possibly damaging	0.88
R1897:Vmn2r87	UTSW	10	130,307,829 (GRCm39)	missense	probably damaging	1.00
R2360:Vmn2r87	UTSW	10	130,315,631 (GRCm39)	missense	probably damaging	0.99
R2909:Vmn2r87	UTSW	10	130,314,865 (GRCm39)	missense	probably damaging	0.99
R3874:Vmn2r87	UTSW	10	130,315,856 (GRCm39)	missense	possibly damaging	0.62
R4113:Vmn2r87	UTSW	10	130,315,691 (GRCm39)	missense	probably benign
R4190:Vmn2r87	UTSW	10	130,308,556 (GRCm39)	missense	probably damaging	1.00
R4197:Vmn2r87	UTSW	10	130,315,779 (GRCm39)	missense	possibly damaging	0.55
R4201:Vmn2r87	UTSW	10	130,308,448 (GRCm39)	missense	probably benign	0.03
R4202:Vmn2r87	UTSW	10	130,308,448 (GRCm39)	missense	probably benign	0.03
R4368:Vmn2r87	UTSW	10	130,315,676 (GRCm39)	missense	probably benign	0.44
R4485:Vmn2r87	UTSW	10	130,315,678 (GRCm39)	nonsense	probably null
R4537:Vmn2r87	UTSW	10	130,308,054 (GRCm39)	missense	probably benign	0.12
R4590:Vmn2r87	UTSW	10	130,315,014 (GRCm39)	missense	possibly damaging	0.69
R4752:Vmn2r87	UTSW	10	130,314,336 (GRCm39)	nonsense	probably null
R4873:Vmn2r87	UTSW	10	130,308,367 (GRCm39)	missense	probably damaging	1.00
R4875:Vmn2r87	UTSW	10	130,308,367 (GRCm39)	missense	probably damaging	1.00
R4923:Vmn2r87	UTSW	10	130,314,435 (GRCm39)	missense	probably damaging	0.99
R4970:Vmn2r87	UTSW	10	130,314,422 (GRCm39)	missense	probably damaging	1.00
R5049:Vmn2r87	UTSW	10	130,308,298 (GRCm39)	missense	probably damaging	0.96
R5112:Vmn2r87	UTSW	10	130,314,422 (GRCm39)	missense	probably damaging	1.00
R5187:Vmn2r87	UTSW	10	130,333,208 (GRCm39)	missense	probably null	0.99
R5618:Vmn2r87	UTSW	10	130,315,817 (GRCm39)	missense	probably damaging	1.00
R6057:Vmn2r87	UTSW	10	130,308,226 (GRCm39)	missense	probably benign	0.02
R6220:Vmn2r87	UTSW	10	130,315,807 (GRCm39)	missense	probably benign	0.01
R6383:Vmn2r87	UTSW	10	130,314,869 (GRCm39)	missense	probably damaging	1.00
R6576:Vmn2r87	UTSW	10	130,314,654 (GRCm39)	missense	probably benign	0.05
R6742:Vmn2r87	UTSW	10	130,308,396 (GRCm39)	missense	probably damaging	1.00
R7086:Vmn2r87	UTSW	10	130,333,178 (GRCm39)	missense	probably benign	0.00
R7162:Vmn2r87	UTSW	10	130,313,416 (GRCm39)	missense	probably benign	0.08
R7419:Vmn2r87	UTSW	10	130,307,992 (GRCm39)	missense	probably damaging	1.00
R7425:Vmn2r87	UTSW	10	130,314,761 (GRCm39)	missense	probably damaging	1.00
R7443:Vmn2r87	UTSW	10	130,308,588 (GRCm39)	missense	probably damaging	1.00
R7571:Vmn2r87	UTSW	10	130,314,940 (GRCm39)	missense	probably damaging	0.99
R7663:Vmn2r87	UTSW	10	130,308,054 (GRCm39)	missense	probably damaging	0.97
R7716:Vmn2r87	UTSW	10	130,308,018 (GRCm39)	missense	probably benign	0.09
R7793:Vmn2r87	UTSW	10	130,313,413 (GRCm39)	missense	probably benign	0.05
R7806:Vmn2r87	UTSW	10	130,315,679 (GRCm39)	missense	probably benign
R7841:Vmn2r87	UTSW	10	130,333,095 (GRCm39)	missense	probably benign	0.31
R8326:Vmn2r87	UTSW	10	130,308,180 (GRCm39)	missense	possibly damaging	0.67
R8411:Vmn2r87	UTSW	10	130,308,126 (GRCm39)	missense	probably damaging	0.99
R8445:Vmn2r87	UTSW	10	130,313,335 (GRCm39)	missense	probably damaging	1.00
R8540:Vmn2r87	UTSW	10	130,314,762 (GRCm39)	missense	possibly damaging	0.74
R8892:Vmn2r87	UTSW	10	130,308,105 (GRCm39)	missense	probably damaging	0.99
R9093:Vmn2r87	UTSW	10	130,308,165 (GRCm39)	missense	probably benign	0.08
R9623:Vmn2r87	UTSW	10	130,315,794 (GRCm39)	missense	probably damaging	1.00
R9667:Vmn2r87	UTSW	10	130,314,776 (GRCm39)	missense	probably damaging	1.00
R9797:Vmn2r87	UTSW	10	130,312,064 (GRCm39)	missense	probably benign	0.44
R9797:Vmn2r87	UTSW	10	130,308,138 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r87	UTSW	10	130,308,183 (GRCm39)	missense	probably damaging	0.98
Z1176:Vmn2r87	UTSW	10	130,307,713 (GRCm39)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- GGGAAGTCTGAATATCTGGAAATTG -3'
(R):5'- TGGAATGGACAGCACTGCAC -3'

Sequencing Primer
(F):5'- CTCAATAAAATGTGTGGCCTTAACC -3'
(R):5'- CAACTATGACATGGCCCTGAGTG -3'

Posted On

2018-03-15