Incidental Mutation 'R7399:Hnmt'
ID 574001
Institutional Source Beutler Lab
Gene Symbol Hnmt
Ensembl Gene ENSMUSG00000026986
Gene Name histamine N-methyltransferase
Synonyms 1500031F01Rik
MMRRC Submission 045481-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.093) question?
Stock # R7399 (G1)
Quality Score 225.009
Status Validated
Chromosome 2
Chromosomal Location 24002910-24049394 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to A at 24003880 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Threonine to Serine at position 201 (T201S)
Ref Sequence ENSEMBL: ENSMUSP00000062747 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000051416] [ENSMUST00000114497] [ENSMUST00000114498]
AlphaFold Q91VF2
Predicted Effect probably benign
Transcript: ENSMUST00000051416
AA Change: T201S

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)
SMART Domains Protein: ENSMUSP00000062747
Gene: ENSMUSG00000026986
AA Change: T201S

DomainStartEndE-ValueType
Pfam:Methyltransf_23 30 218 3.6e-16 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000114497
AA Change: T201S

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)
SMART Domains Protein: ENSMUSP00000110141
Gene: ENSMUSG00000026986
AA Change: T201S

DomainStartEndE-ValueType
Pfam:Methyltransf_23 29 218 4.3e-18 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000114498
AA Change: T201S

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)
SMART Domains Protein: ENSMUSP00000110142
Gene: ENSMUSG00000026986
AA Change: T201S

DomainStartEndE-ValueType
Pfam:Methyltransf_23 30 218 3.6e-16 PFAM
Meta Mutation Damage Score 0.1206 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency 99% (74/75)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit elevated histamine levels in the brain, increased aggression, hypoactivity and altered sleep-wake cycle. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 74 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acaca T G 11: 84,260,679 (GRCm38) V801G possibly damaging Het
Actbl2 T A 13: 111,255,593 (GRCm38) M154K probably benign Het
Adam7 A T 14: 68,504,466 (GRCm38) probably null Het
Arfgef1 T A 1: 10,180,897 (GRCm38) T888S probably benign Het
AW554918 C T 18: 25,169,060 (GRCm38) P10L possibly damaging Het
Bcap29 A G 12: 31,630,882 (GRCm38) I35T probably damaging Het
Bhlhe40 TG TGG 6: 108,664,857 (GRCm38) 254 probably null Het
Cby2 A G 14: 75,592,637 (GRCm38) S39P probably benign Het
Cdc25b A G 2: 131,194,654 (GRCm38) D458G probably damaging Het
Cdc42bpb T C 12: 111,305,667 (GRCm38) K1104R probably benign Het
Cep89 A G 7: 35,438,378 (GRCm38) N729S probably damaging Het
Clec4a4 T A 6: 122,991,829 (GRCm38) M51K possibly damaging Het
Dcdc2b A G 4: 129,609,629 (GRCm38) L270P probably damaging Het
Dennd5b G T 6: 149,036,483 (GRCm38) H639N probably damaging Het
Dnah11 T C 12: 118,125,785 (GRCm38) E1182G probably damaging Het
Dnah11 T G 12: 118,027,477 (GRCm38) T2385P probably benign Het
Dok7 T C 5: 35,066,471 (GRCm38) V81A probably damaging Het
Dtx1 T A 5: 120,682,393 (GRCm38) M494L possibly damaging Het
Foxj1 A T 11: 116,332,254 (GRCm38) L241Q possibly damaging Het
Gbp10 G A 5: 105,236,149 (GRCm38) probably benign Het
Jup G T 11: 100,378,351 (GRCm38) T412K possibly damaging Het
Kcnh2 G A 5: 24,322,059 (GRCm38) S954F probably damaging Het
Klhdc7b A C 15: 89,388,644 (GRCm38) K585T possibly damaging Het
Klk7 T A 7: 43,812,000 (GRCm38) S14T probably benign Het
Lama4 G A 10: 39,047,948 (GRCm38) E451K probably damaging Het
Ldhb A G 6: 142,495,673 (GRCm38) C164R probably damaging Het
Malrd1 G A 2: 15,610,090 (GRCm38) D239N Het
Mgam T A 6: 40,666,854 (GRCm38) V572E probably damaging Het
Mrgprb2 G T 7: 48,552,142 (GRCm38) N278K probably damaging Het
Msto1 A G 3: 88,911,823 (GRCm38) Y206H probably damaging Het
Myo6 A G 9: 80,262,291 (GRCm38) S467G unknown Het
Mysm1 T A 4: 94,961,727 (GRCm38) I447L probably benign Het
Nav3 T C 10: 109,852,934 (GRCm38) E494G possibly damaging Het
Nol10 T G 12: 17,402,173 (GRCm38) V376G probably damaging Het
Nup205 T A 6: 35,214,676 (GRCm38) I1032N probably damaging Het
Oog2 A G 4: 144,195,281 (GRCm38) K254E probably benign Het
Or10ac1 A G 6: 42,538,728 (GRCm38) F98S possibly damaging Het
Or52z12 A T 7: 103,584,381 (GRCm38) I120L possibly damaging Het
Or5b98 A G 19: 12,954,447 (GRCm38) N286S probably damaging Het
Or5d41 A T 2: 88,225,022 (GRCm38) Y3* probably null Het
Or5g25 T A 2: 85,647,424 (GRCm38) D299V possibly damaging Het
Or5g27 A G 2: 85,579,296 (GRCm38) D19G probably benign Het
Or6b1 T G 6: 42,838,746 (GRCm38) Y288* probably null Het
Or6c6 A T 10: 129,350,557 (GRCm38) probably benign Het
Or8h10 G A 2: 86,978,157 (GRCm38) T213I probably benign Het
Osbpl11 T A 16: 33,236,279 (GRCm38) D694E probably benign Het
Pcm1 T A 8: 41,293,510 (GRCm38) Y1210N probably benign Het
Pdxk T C 10: 78,440,863 (GRCm38) M293V probably benign Het
Plcb3 T C 19: 6,962,867 (GRCm38) I451V probably benign Het
Plekhm2 A G 4: 141,634,376 (GRCm38) F272S probably damaging Het
Pramel32 C A 4: 88,627,965 (GRCm38) R380L probably benign Het
Ptgdr A T 14: 44,858,232 (GRCm38) probably null Het
Ptprf C T 4: 118,226,523 (GRCm38) V788I probably benign Het
Ralbp1 C T 17: 65,854,148 (GRCm38) V467I probably benign Het
Ralgds A C 2: 28,543,655 (GRCm38) Q229P possibly damaging Het
Recql T C 6: 142,374,884 (GRCm38) D146G probably damaging Het
Reln T C 5: 22,051,367 (GRCm38) N493S probably damaging Het
Rfxank C T 8: 70,135,286 (GRCm38) probably null Het
Scn5a A T 9: 119,486,530 (GRCm38) M1704K probably damaging Het
Slc7a7 G T 14: 54,374,268 (GRCm38) A316E possibly damaging Het
Slco1a6 A T 6: 142,091,068 (GRCm38) C538S probably benign Het
Stard6 T C 18: 70,498,647 (GRCm38) probably null Het
Strip2 T A 6: 29,927,613 (GRCm38) M219K possibly damaging Het
Tcam1 T C 11: 106,284,085 (GRCm38) V122A probably damaging Het
Tha1 A G 11: 117,869,690 (GRCm38) V236A possibly damaging Het
Tmem201 A T 4: 149,731,097 (GRCm38) I132N possibly damaging Het
Tnc T C 4: 64,020,657 (GRCm38) probably benign Het
Vmn1r42 T C 6: 89,845,513 (GRCm38) T25A probably benign Het
Vmn2r94 T A 17: 18,244,503 (GRCm38) probably null Het
Wdfy4 A C 14: 33,068,906 (GRCm38) V2188G Het
Zfp623 T C 15: 75,947,398 (GRCm38) S68P probably damaging Het
Zfp950 A T 19: 61,119,155 (GRCm38) C497S probably damaging Het
Zkscan2 C T 7: 123,480,104 (GRCm38) E877K probably damaging Het
Zp3r C A 1: 130,577,053 (GRCm38) V536L probably damaging Het
Other mutations in Hnmt
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00479:Hnmt APN 2 24,003,884 (GRCm38) nonsense probably null
IGL00857:Hnmt APN 2 24,003,783 (GRCm38) missense probably benign 0.04
IGL01315:Hnmt APN 2 24,019,168 (GRCm38) missense probably benign 0.02
IGL02205:Hnmt APN 2 24,019,145 (GRCm38) missense probably damaging 1.00
IGL02647:Hnmt APN 2 24,014,307 (GRCm38) missense possibly damaging 0.79
IGL03123:Hnmt APN 2 24,019,159 (GRCm38) missense probably benign
IGL03137:Hnmt APN 2 24,048,739 (GRCm38) missense probably damaging 0.99
R0018:Hnmt UTSW 2 24,003,628 (GRCm38) missense possibly damaging 0.69
R1959:Hnmt UTSW 2 24,003,882 (GRCm38) missense possibly damaging 0.84
R2106:Hnmt UTSW 2 24,019,118 (GRCm38) missense probably benign 0.19
R2426:Hnmt UTSW 2 24,019,155 (GRCm38) missense probably benign 0.11
R4024:Hnmt UTSW 2 24,003,765 (GRCm38) missense probably benign
R4590:Hnmt UTSW 2 24,019,099 (GRCm38) splice site probably null
R5643:Hnmt UTSW 2 24,014,239 (GRCm38) missense probably damaging 1.00
R5644:Hnmt UTSW 2 24,014,239 (GRCm38) missense probably damaging 1.00
R6240:Hnmt UTSW 2 24,014,269 (GRCm38) missense probably benign 0.00
R7153:Hnmt UTSW 2 24,014,341 (GRCm38) missense probably damaging 0.98
R7359:Hnmt UTSW 2 24,048,719 (GRCm38) missense probably benign
R8290:Hnmt UTSW 2 24,003,884 (GRCm38) nonsense probably null
R8463:Hnmt UTSW 2 24,048,824 (GRCm38) start codon destroyed probably null 1.00
R9183:Hnmt UTSW 2 24,003,643 (GRCm38) missense probably benign 0.03
R9524:Hnmt UTSW 2 24,003,868 (GRCm38) missense possibly damaging 0.92
Predicted Primers PCR Primer
(F):5'- GCTTTGAGATCCAGTGGTGC -3'
(R):5'- AAATGTCTTTCACCACACTTCAGC -3'

Sequencing Primer
(F):5'- ATCCAGTGGTGCTGTTTTGCTAAAG -3'
(R):5'- GCTACTTGAATTAAGCTTCACAAAGG -3'
Posted On 2019-09-13