Mutagenetix > Incidental Mutation

Incidental Mutation 'R7482:Vmn2r27'

579853

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r27

Ensembl Gene

ENSMUSG00000072778

Gene Name

vomeronasal 2, receptor27

Synonyms

EG232367

MMRRC Submission

045556-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R7482 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

124168555-124208743 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 124201220 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 246 (F246L)

Ref Sequence

ENSEMBL: ENSMUSP00000098528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100968]

AlphaFold

D3YUK6

Predicted Effect

probably damaging
Transcript: ENSMUST00000100968
AA Change: F246L

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000098528
Gene: ENSMUSG00000072778
AA Change: F246L

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	81	475	1.1e-27	PFAM
Pfam:NCD3G	519	570	1.3e-18	PFAM
Pfam:7tm_3	603	838	2.6e-50	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (37/37)

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actbl2	G	A	13: 111,392,673 (GRCm39)	R336H	probably damaging	Het
Akap9	C	G	5: 4,018,745 (GRCm39)	H1109D	probably benign	Het
Ap2a2	C	T	7: 141,182,210 (GRCm39)	P180S	possibly damaging	Het
Arfgef2	T	C	2: 166,693,199 (GRCm39)		probably null	Het
Arhgef25	A	T	10: 127,021,540 (GRCm39)	M226K	probably damaging	Het
Brd7	T	C	8: 89,088,254 (GRCm39)	D45G	probably damaging	Het
Bsn	C	A	9: 107,990,728 (GRCm39)	V1675F	probably damaging	Het
Chtf18	C	A	17: 25,938,963 (GRCm39)	R820L	possibly damaging	Het
Cldn7	A	G	11: 69,856,865 (GRCm39)	D38G	possibly damaging	Het
Clpb	T	C	7: 101,435,926 (GRCm39)	V615A	possibly damaging	Het
Cntnap4	T	C	8: 113,460,194 (GRCm39)		probably null	Het
Dchs2	T	A	3: 83,156,032 (GRCm39)	S798T	possibly damaging	Het
Ect2l	A	T	10: 18,044,202 (GRCm39)	M311K	probably benign	Het
Hectd4	T	A	5: 121,501,941 (GRCm39)	C4225S	possibly damaging	Het
Hecw2	T	C	1: 54,079,629 (GRCm39)	H8R	probably damaging	Het
Hif3a	G	A	7: 16,776,560 (GRCm39)	T462I	possibly damaging	Het
Itgb2l	T	C	16: 96,228,033 (GRCm39)	E490G	probably benign	Het
Jakmip3	T	A	7: 138,627,228 (GRCm39)	C411S	possibly damaging	Het
Klhl24	A	G	16: 19,933,405 (GRCm39)	T339A	possibly damaging	Het
Mctp1	A	T	13: 76,889,579 (GRCm39)		probably null	Het
Mlf1	T	A	3: 67,300,227 (GRCm39)	H81Q	probably benign	Het
Muc4	T	A	16: 32,587,324 (GRCm39)	Y652N		Het
Myo9b	A	G	8: 71,795,442 (GRCm39)	S804G	probably benign	Het
Or8b1	T	G	9: 38,399,747 (GRCm39)	C141G	probably damaging	Het
Pramel58	T	C	5: 94,830,739 (GRCm39)	I79T	possibly damaging	Het
Rab11fip5	T	C	6: 85,317,760 (GRCm39)	E1043G	probably benign	Het
Radil	A	G	5: 142,472,518 (GRCm39)	V941A	probably benign	Het
Senp8	A	G	9: 59,644,943 (GRCm39)	V71A	probably damaging	Het
Sh2d4a	G	A	8: 68,749,328 (GRCm39)	A121T	probably benign	Het
Stx17	A	G	4: 48,181,722 (GRCm39)	D297G	possibly damaging	Het
Tas2r105	A	T	6: 131,663,972 (GRCm39)	M152K	probably benign	Het
Tlr11	G	T	14: 50,600,456 (GRCm39)	C814F	probably damaging	Het
Tsc22d1	T	C	14: 76,655,927 (GRCm39)	V802A	probably benign	Het
Vmn1r234	A	G	17: 21,449,637 (GRCm39)	N184D	probably benign	Het
Vmn2r114	T	C	17: 23,510,468 (GRCm39)	K671E	probably damaging	Het
Xpo1	T	G	11: 23,232,544 (GRCm39)	L355V	probably benign	Het

Other mutations in Vmn2r27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01285:Vmn2r27	APN	6	124,169,370 (GRCm39)	missense	possibly damaging	0.86
IGL01388:Vmn2r27	APN	6	124,200,791 (GRCm39)	missense	possibly damaging	0.55
IGL01923:Vmn2r27	APN	6	124,177,484 (GRCm39)	missense	probably benign	0.20
IGL01954:Vmn2r27	APN	6	124,169,207 (GRCm39)	missense	probably damaging	1.00
IGL02105:Vmn2r27	APN	6	124,174,308 (GRCm39)	splice site	probably benign
IGL02586:Vmn2r27	APN	6	124,201,434 (GRCm39)	nonsense	probably null
IGL03130:Vmn2r27	APN	6	124,169,276 (GRCm39)	missense	possibly damaging	0.82
IGL03330:Vmn2r27	APN	6	124,207,139 (GRCm39)	nonsense	probably null
R0124:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0234:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0234:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0384:Vmn2r27	UTSW	6	124,200,871 (GRCm39)	missense	probably benign	0.01
R0582:Vmn2r27	UTSW	6	124,201,249 (GRCm39)	missense	probably benign	0.02
R0733:Vmn2r27	UTSW	6	124,169,147 (GRCm39)	missense	probably benign	0.18
R0738:Vmn2r27	UTSW	6	124,200,661 (GRCm39)	missense	possibly damaging	0.48
R0835:Vmn2r27	UTSW	6	124,177,583 (GRCm39)	missense	probably damaging	0.99
R1183:Vmn2r27	UTSW	6	124,177,491 (GRCm39)	missense	probably benign
R1401:Vmn2r27	UTSW	6	124,168,591 (GRCm39)	nonsense	probably null
R1484:Vmn2r27	UTSW	6	124,177,474 (GRCm39)	missense	probably damaging	0.96
R1536:Vmn2r27	UTSW	6	124,177,649 (GRCm39)	missense	probably damaging	1.00
R1539:Vmn2r27	UTSW	6	124,168,730 (GRCm39)	missense	probably damaging	1.00
R1565:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1595:Vmn2r27	UTSW	6	124,208,574 (GRCm39)	missense	probably benign	0.00
R1614:Vmn2r27	UTSW	6	124,200,893 (GRCm39)	missense	probably benign	0.01
R1742:Vmn2r27	UTSW	6	124,177,636 (GRCm39)	missense	possibly damaging	0.48
R1816:Vmn2r27	UTSW	6	124,207,330 (GRCm39)	nonsense	probably null
R1822:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1824:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1870:Vmn2r27	UTSW	6	124,201,170 (GRCm39)	missense	probably benign	0.11
R1942:Vmn2r27	UTSW	6	124,200,722 (GRCm39)	missense	probably damaging	1.00
R1962:Vmn2r27	UTSW	6	124,200,793 (GRCm39)	missense	possibly damaging	0.70
R2069:Vmn2r27	UTSW	6	124,201,442 (GRCm39)	missense	probably damaging	1.00
R2075:Vmn2r27	UTSW	6	124,177,510 (GRCm39)	missense	possibly damaging	0.85
R2379:Vmn2r27	UTSW	6	124,201,342 (GRCm39)	missense	possibly damaging	0.89
R3748:Vmn2r27	UTSW	6	124,207,351 (GRCm39)	missense	probably benign	0.35
R4384:Vmn2r27	UTSW	6	124,201,115 (GRCm39)	missense	probably benign	0.05
R4392:Vmn2r27	UTSW	6	124,207,135 (GRCm39)	missense	probably benign	0.01
R4758:Vmn2r27	UTSW	6	124,208,596 (GRCm39)	missense	possibly damaging	0.87
R5018:Vmn2r27	UTSW	6	124,201,141 (GRCm39)	missense	probably benign	0.02
R5235:Vmn2r27	UTSW	6	124,169,013 (GRCm39)	missense	probably damaging	0.99
R5718:Vmn2r27	UTSW	6	124,169,103 (GRCm39)	missense	possibly damaging	0.66
R5859:Vmn2r27	UTSW	6	124,177,647 (GRCm39)	missense	probably damaging	1.00
R5958:Vmn2r27	UTSW	6	124,208,686 (GRCm39)	missense	probably benign	0.00
R6044:Vmn2r27	UTSW	6	124,208,731 (GRCm39)	missense	probably benign
R6086:Vmn2r27	UTSW	6	124,168,958 (GRCm39)	missense	probably damaging	1.00
R6396:Vmn2r27	UTSW	6	124,201,125 (GRCm39)	nonsense	probably null
R6546:Vmn2r27	UTSW	6	124,169,369 (GRCm39)	missense	possibly damaging	0.49
R6746:Vmn2r27	UTSW	6	124,177,552 (GRCm39)	missense	possibly damaging	0.47
R6976:Vmn2r27	UTSW	6	124,201,312 (GRCm39)	nonsense	probably null
R7091:Vmn2r27	UTSW	6	124,200,904 (GRCm39)	missense	possibly damaging	0.85
R7145:Vmn2r27	UTSW	6	124,168,711 (GRCm39)	missense	probably benign
R7176:Vmn2r27	UTSW	6	124,168,995 (GRCm39)	missense	probably benign	0.01
R7382:Vmn2r27	UTSW	6	124,174,276 (GRCm39)	missense	probably damaging	1.00
R7853:Vmn2r27	UTSW	6	124,168,980 (GRCm39)	missense	probably damaging	1.00
R7859:Vmn2r27	UTSW	6	124,201,201 (GRCm39)	missense	probably benign	0.00
R7959:Vmn2r27	UTSW	6	124,169,040 (GRCm39)	missense	probably benign
R8266:Vmn2r27	UTSW	6	124,168,937 (GRCm39)	missense	probably benign	0.00
R8353:Vmn2r27	UTSW	6	124,169,404 (GRCm39)	missense	probably damaging	0.99
R8394:Vmn2r27	UTSW	6	124,168,776 (GRCm39)	missense	possibly damaging	0.71
R8463:Vmn2r27	UTSW	6	124,169,168 (GRCm39)	missense	probably damaging	1.00
R8477:Vmn2r27	UTSW	6	124,201,200 (GRCm39)	missense	probably benign	0.11
R8705:Vmn2r27	UTSW	6	124,207,188 (GRCm39)	missense	probably damaging	1.00
R8752:Vmn2r27	UTSW	6	124,201,018 (GRCm39)	missense	probably benign	0.00
R9109:Vmn2r27	UTSW	6	124,174,224 (GRCm39)	missense	possibly damaging	0.95
R9140:Vmn2r27	UTSW	6	124,169,207 (GRCm39)	missense	probably damaging	1.00
R9157:Vmn2r27	UTSW	6	124,201,244 (GRCm39)	missense	probably benign	0.09
R9431:Vmn2r27	UTSW	6	124,168,856 (GRCm39)	missense	probably damaging	1.00
R9477:Vmn2r27	UTSW	6	124,168,910 (GRCm39)	missense	probably damaging	0.99
R9758:Vmn2r27	UTSW	6	124,168,637 (GRCm39)	missense	possibly damaging	0.89
Z1177:Vmn2r27	UTSW	6	124,168,860 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATCCCAGAATTTCATCCATGTGATC -3'
(R):5'- AGCTCCAGTCTCCTTACCAG -3'

Sequencing Primer
(F):5'- ACCAATCAGAATTTGTGATCAAGAC -3'
(R):5'- ACCAGTTTCCTGTGCACACTG -3'

Posted On

2019-10-07