Mutagenetix > Incidental Mutation

Incidental Mutation 'R0961:Or2g7'

81904

Institutional Source

Beutler Lab

Gene Symbol

Or2g7

Ensembl Gene

ENSMUSG00000094878

Gene Name

olfactory receptor family 2 subfamily G member 7

Synonyms

GA_x6K02T2PSCP-2515350-2516303, MOR256-19, Olfr130

MMRRC Submission

039090-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.119) question?

Stock #

R0961 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

38378064-38379017 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 38378814 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Asparagine at position 251 (Y251N)

Ref Sequence

ENSEMBL: ENSMUSP00000149601 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087129] [ENSMUST00000215726] [ENSMUST00000217390]

AlphaFold

Q8VFC1

Predicted Effect

probably damaging
Transcript: ENSMUST00000087129
AA Change: Y251N

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000084369
Gene: ENSMUSG00000094878
AA Change: Y251N

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	307	1.8e-44	PFAM
Pfam:7TM_GPCR_Srsx	34	223	1.9e-5	PFAM
Pfam:7tm_1	40	289	1.9e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215726
AA Change: Y251N

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

Predicted Effect

probably damaging
Transcript: ENSMUST00000217390
AA Change: Y251N

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.3%
10x: 95.6%
20x: 89.7%

Validation Efficiency

100% (60/60)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921509C19Rik	A	C	2: 151,314,686 (GRCm39)	S331A	probably benign	Het
4930433I11Rik	A	T	7: 40,642,480 (GRCm39)	T141S	probably benign	Het
4933412E24Rik	T	C	15: 59,887,160 (GRCm39)	I427V	probably benign	Het
Abca15	A	T	7: 119,960,208 (GRCm39)	K664*	probably null	Het
Adcy8	A	G	15: 64,626,711 (GRCm39)	V709A	possibly damaging	Het
Aox1	T	A	1: 58,349,230 (GRCm39)	D665E	probably benign	Het
Arhgap22	C	T	14: 33,089,070 (GRCm39)	T352M	probably damaging	Het
Atg9a	G	A	1: 75,163,390 (GRCm39)	L237F	probably damaging	Het
Ccdc178	T	G	18: 22,152,098 (GRCm39)	K672T	possibly damaging	Het
Ccdc63	T	G	5: 122,249,009 (GRCm39)	K440T	possibly damaging	Het
Cd55b	A	T	1: 130,341,813 (GRCm39)	W275R	probably damaging	Het
Col4a3	T	C	1: 82,686,297 (GRCm39)		probably benign	Het
Dmpk	C	G	7: 18,821,195 (GRCm39)	D204E	probably damaging	Het
Egfr	T	C	11: 16,812,964 (GRCm39)	V148A	probably damaging	Het
F11	A	T	8: 45,694,531 (GRCm39)	V610E	probably damaging	Het
Fam83b	A	T	9: 76,398,577 (GRCm39)	I842N	probably damaging	Het
Fbxw9	T	C	8: 85,788,658 (GRCm39)	Y165H	probably benign	Het
Fzd6	C	T	15: 38,889,073 (GRCm39)	L64F	probably damaging	Het
Galntl6	A	T	8: 59,364,374 (GRCm39)	H45Q	probably benign	Het
Gbp7	C	A	3: 142,247,318 (GRCm39)	S276*	probably null	Het
Gnb5	A	T	9: 75,242,933 (GRCm39)	I168F	probably damaging	Het
Gon4l	T	C	3: 88,805,403 (GRCm39)		probably benign	Het
Gpat4	C	T	8: 23,670,927 (GRCm39)	C95Y	probably damaging	Het
Gstm7	T	A	3: 107,834,302 (GRCm39)		probably benign	Het
Hyal4	A	G	6: 24,755,745 (GRCm39)		probably benign	Het
Iqca1	C	A	1: 90,070,453 (GRCm39)	G133V	probably null	Het
Kank4	G	A	4: 98,644,756 (GRCm39)	R999W	probably benign	Het
Kdm2a	A	G	19: 4,379,219 (GRCm39)	V92A	probably benign	Het
Klhl9	T	C	4: 88,639,974 (GRCm39)	D89G	probably benign	Het
Klre1	A	G	6: 129,559,378 (GRCm39)	T103A	probably benign	Het
Lamc1	CGCTGGC	CGC	1: 153,097,392 (GRCm39)		probably null	Het
Lamc1	G	T	1: 153,097,446 (GRCm39)	L1533I	probably benign	Het
Lca5l	T	C	16: 95,962,560 (GRCm39)	H455R	possibly damaging	Het
Lmo7	C	A	14: 102,031,705 (GRCm39)	T33K	probably benign	Het
Lrig1	A	G	6: 94,640,895 (GRCm39)		probably benign	Het
Mep1b	T	A	18: 21,221,786 (GRCm39)	Y245*	probably null	Het
Mettl24	A	G	10: 40,686,615 (GRCm39)	T331A	possibly damaging	Het
Mycbp2	A	C	14: 103,422,271 (GRCm39)	D2467E	probably damaging	Het
Myo15b	T	C	11: 115,773,280 (GRCm39)	S1871P	probably benign	Het
Ncbp1	T	C	4: 46,165,193 (GRCm39)	L502P	possibly damaging	Het
Npr1	T	C	3: 90,366,028 (GRCm39)	N588D	possibly damaging	Het
Or8k16	A	T	2: 85,519,790 (GRCm39)	T6S	probably benign	Het
Oxtr	C	T	6: 112,454,138 (GRCm39)	R42Q	probably benign	Het
Phactr4	A	G	4: 132,105,731 (GRCm39)	S112P	probably benign	Het
R3hdm1	C	T	1: 128,121,333 (GRCm39)	T279I	probably benign	Het
Rere	A	G	4: 150,699,829 (GRCm39)		probably benign	Het
Ryr1	T	A	7: 28,709,122 (GRCm39)	E4779V	unknown	Het
Sh2d4b	A	G	14: 40,596,139 (GRCm39)	V81A	probably benign	Het
Slc10a5	T	C	3: 10,399,484 (GRCm39)	H392R	probably benign	Het
Slc26a4	T	C	12: 31,585,618 (GRCm39)	T477A	probably benign	Het
Spata31d1b	T	C	13: 59,865,618 (GRCm39)	V922A	possibly damaging	Het
Sptan1	T	A	2: 29,870,075 (GRCm39)		probably null	Het
Stard9	A	G	2: 120,523,920 (GRCm39)	D705G	probably benign	Het
Tdpoz3	T	A	3: 93,734,188 (GRCm39)	S288T	probably benign	Het
Tsga10	A	G	1: 37,800,509 (GRCm39)		probably null	Het
Usp18	G	A	6: 121,238,452 (GRCm39)	A200T	probably benign	Het
Zfp759	A	T	13: 67,287,927 (GRCm39)	T493S	probably benign	Het

Other mutations in Or2g7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01527:Or2g7	APN	17	38,378,986 (GRCm39)	missense	probably benign
IGL02158:Or2g7	APN	17	38,378,158 (GRCm39)	missense	probably damaging	0.97
IGL03172:Or2g7	APN	17	38,378,275 (GRCm39)	missense	probably damaging	0.99
R0389:Or2g7	UTSW	17	38,378,562 (GRCm39)	missense	possibly damaging	0.58
R0448:Or2g7	UTSW	17	38,378,563 (GRCm39)	missense	probably benign	0.00
R0616:Or2g7	UTSW	17	38,378,131 (GRCm39)	missense	probably damaging	1.00
R1789:Or2g7	UTSW	17	38,378,839 (GRCm39)	missense	probably damaging	1.00
R2108:Or2g7	UTSW	17	38,378,746 (GRCm39)	missense	possibly damaging	0.82
R4600:Or2g7	UTSW	17	38,378,853 (GRCm39)	missense	probably damaging	0.99
R4977:Or2g7	UTSW	17	38,378,638 (GRCm39)	missense	possibly damaging	0.67
R5120:Or2g7	UTSW	17	38,378,157 (GRCm39)	missense	probably damaging	0.97
R5930:Or2g7	UTSW	17	38,378,641 (GRCm39)	missense	probably benign	0.11
R6273:Or2g7	UTSW	17	38,378,686 (GRCm39)	missense	probably damaging	1.00
R6636:Or2g7	UTSW	17	38,378,115 (GRCm39)	missense	probably damaging	0.98
R6637:Or2g7	UTSW	17	38,378,115 (GRCm39)	missense	probably damaging	0.98
R7030:Or2g7	UTSW	17	38,378,948 (GRCm39)	missense	probably benign	0.13
R7045:Or2g7	UTSW	17	38,378,862 (GRCm39)	missense	probably benign	0.01
R7175:Or2g7	UTSW	17	38,378,370 (GRCm39)	missense	probably damaging	0.96
R7359:Or2g7	UTSW	17	38,378,506 (GRCm39)	nonsense	probably null
R7762:Or2g7	UTSW	17	38,378,566 (GRCm39)	missense	probably damaging	1.00
R7980:Or2g7	UTSW	17	38,378,412 (GRCm39)	missense	possibly damaging	0.64
R8050:Or2g7	UTSW	17	38,378,370 (GRCm39)	missense	probably damaging	1.00
R9035:Or2g7	UTSW	17	38,378,179 (GRCm39)	missense	probably benign	0.00
X0019:Or2g7	UTSW	17	38,378,613 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGCAGCTATTCATGGCTTCCTTGG -3'
(R):5'- AGTGATTTCCTGGCTTGTAGCACTC -3'

Sequencing Primer
(F):5'- GGCTTCCTTGGTGCTGC -3'
(R):5'- GAGCATCTATCTCATGGTGAGCC -3'

Posted On

2013-11-08