Mutagenetix > Incidental Mutation

Incidental Mutation 'R0961:Gstm7'

81867

Institutional Source

Beutler Lab

Gene Symbol

Gstm7

Ensembl Gene

ENSMUSG00000004035

Gene Name

glutathione S-transferase, mu 7

Synonyms

Cd203c, 0610005A07Rik

MMRRC Submission

039090-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.194) question?

Stock #

R0961 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

107833650-107839133 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

T to A at 107834302 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000122567 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000004137] [ENSMUST00000106687] [ENSMUST00000106688] [ENSMUST00000124215] [ENSMUST00000133947]

AlphaFold

Q80W21

Predicted Effect

probably benign
Transcript: ENSMUST00000004137

SMART Domains

Protein: ENSMUSP00000004137
Gene: ENSMUSG00000004035

Domain	Start	End	E-Value	Type
Pfam:GST_N	3	82	2.2e-23	PFAM
Pfam:GST_C_3	42	190	1.2e-9	PFAM
Pfam:GST_C	104	191	5.3e-15	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000106687

SMART Domains

Protein: ENSMUSP00000102298
Gene: ENSMUSG00000004035

Domain	Start	End	E-Value	Type
Pfam:GST_N	3	82	6.8e-24	PFAM
Pfam:GST_N_3	11	93	1.1e-6	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000106688

SMART Domains

Protein: ENSMUSP00000102299
Gene: ENSMUSG00000004035

Domain	Start	End	E-Value	Type
Pfam:GST_N_3	4	89	8.8e-7	PFAM
Pfam:GST_N	5	78	4.2e-19	PFAM
Pfam:GST_C	100	188	5.6e-16	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000124215

SMART Domains

Protein: ENSMUSP00000118707
Gene: ENSMUSG00000004035

Domain	Start	End	E-Value	Type
Pfam:GST_N	1	72	8.6e-20	PFAM
Pfam:GST_N_3	3	83	4e-7	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000133947

SMART Domains

Protein: ENSMUSP00000122567
Gene: ENSMUSG00000004035

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:GST_N	45	123	2.6e-19	PFAM
Pfam:GST_N_3	54	134	1.4e-6	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.3%
10x: 95.6%
20x: 89.7%

Validation Efficiency

100% (60/60)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921509C19Rik	A	C	2: 151,314,686 (GRCm39)	S331A	probably benign	Het
4930433I11Rik	A	T	7: 40,642,480 (GRCm39)	T141S	probably benign	Het
4933412E24Rik	T	C	15: 59,887,160 (GRCm39)	I427V	probably benign	Het
Abca15	A	T	7: 119,960,208 (GRCm39)	K664*	probably null	Het
Adcy8	A	G	15: 64,626,711 (GRCm39)	V709A	possibly damaging	Het
Aox1	T	A	1: 58,349,230 (GRCm39)	D665E	probably benign	Het
Arhgap22	C	T	14: 33,089,070 (GRCm39)	T352M	probably damaging	Het
Atg9a	G	A	1: 75,163,390 (GRCm39)	L237F	probably damaging	Het
Ccdc178	T	G	18: 22,152,098 (GRCm39)	K672T	possibly damaging	Het
Ccdc63	T	G	5: 122,249,009 (GRCm39)	K440T	possibly damaging	Het
Cd55b	A	T	1: 130,341,813 (GRCm39)	W275R	probably damaging	Het
Col4a3	T	C	1: 82,686,297 (GRCm39)		probably benign	Het
Dmpk	C	G	7: 18,821,195 (GRCm39)	D204E	probably damaging	Het
Egfr	T	C	11: 16,812,964 (GRCm39)	V148A	probably damaging	Het
F11	A	T	8: 45,694,531 (GRCm39)	V610E	probably damaging	Het
Fam83b	A	T	9: 76,398,577 (GRCm39)	I842N	probably damaging	Het
Fbxw9	T	C	8: 85,788,658 (GRCm39)	Y165H	probably benign	Het
Fzd6	C	T	15: 38,889,073 (GRCm39)	L64F	probably damaging	Het
Galntl6	A	T	8: 59,364,374 (GRCm39)	H45Q	probably benign	Het
Gbp7	C	A	3: 142,247,318 (GRCm39)	S276*	probably null	Het
Gnb5	A	T	9: 75,242,933 (GRCm39)	I168F	probably damaging	Het
Gon4l	T	C	3: 88,805,403 (GRCm39)		probably benign	Het
Gpat4	C	T	8: 23,670,927 (GRCm39)	C95Y	probably damaging	Het
Hyal4	A	G	6: 24,755,745 (GRCm39)		probably benign	Het
Iqca1	C	A	1: 90,070,453 (GRCm39)	G133V	probably null	Het
Kank4	G	A	4: 98,644,756 (GRCm39)	R999W	probably benign	Het
Kdm2a	A	G	19: 4,379,219 (GRCm39)	V92A	probably benign	Het
Klhl9	T	C	4: 88,639,974 (GRCm39)	D89G	probably benign	Het
Klre1	A	G	6: 129,559,378 (GRCm39)	T103A	probably benign	Het
Lamc1	CGCTGGC	CGC	1: 153,097,392 (GRCm39)		probably null	Het
Lamc1	G	T	1: 153,097,446 (GRCm39)	L1533I	probably benign	Het
Lca5l	T	C	16: 95,962,560 (GRCm39)	H455R	possibly damaging	Het
Lmo7	C	A	14: 102,031,705 (GRCm39)	T33K	probably benign	Het
Lrig1	A	G	6: 94,640,895 (GRCm39)		probably benign	Het
Mep1b	T	A	18: 21,221,786 (GRCm39)	Y245*	probably null	Het
Mettl24	A	G	10: 40,686,615 (GRCm39)	T331A	possibly damaging	Het
Mycbp2	A	C	14: 103,422,271 (GRCm39)	D2467E	probably damaging	Het
Myo15b	T	C	11: 115,773,280 (GRCm39)	S1871P	probably benign	Het
Ncbp1	T	C	4: 46,165,193 (GRCm39)	L502P	possibly damaging	Het
Npr1	T	C	3: 90,366,028 (GRCm39)	N588D	possibly damaging	Het
Or2g7	T	A	17: 38,378,814 (GRCm39)	Y251N	probably damaging	Het
Or8k16	A	T	2: 85,519,790 (GRCm39)	T6S	probably benign	Het
Oxtr	C	T	6: 112,454,138 (GRCm39)	R42Q	probably benign	Het
Phactr4	A	G	4: 132,105,731 (GRCm39)	S112P	probably benign	Het
R3hdm1	C	T	1: 128,121,333 (GRCm39)	T279I	probably benign	Het
Rere	A	G	4: 150,699,829 (GRCm39)		probably benign	Het
Ryr1	T	A	7: 28,709,122 (GRCm39)	E4779V	unknown	Het
Sh2d4b	A	G	14: 40,596,139 (GRCm39)	V81A	probably benign	Het
Slc10a5	T	C	3: 10,399,484 (GRCm39)	H392R	probably benign	Het
Slc26a4	T	C	12: 31,585,618 (GRCm39)	T477A	probably benign	Het
Spata31d1b	T	C	13: 59,865,618 (GRCm39)	V922A	possibly damaging	Het
Sptan1	T	A	2: 29,870,075 (GRCm39)		probably null	Het
Stard9	A	G	2: 120,523,920 (GRCm39)	D705G	probably benign	Het
Tdpoz3	T	A	3: 93,734,188 (GRCm39)	S288T	probably benign	Het
Tsga10	A	G	1: 37,800,509 (GRCm39)		probably null	Het
Usp18	G	A	6: 121,238,452 (GRCm39)	A200T	probably benign	Het
Zfp759	A	T	13: 67,287,927 (GRCm39)	T493S	probably benign	Het

Other mutations in Gstm7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02215:Gstm7	APN	3	107,837,594 (GRCm39)	missense	possibly damaging	0.93
PIT4142001:Gstm7	UTSW	3	107,838,799 (GRCm39)	frame shift	probably null
R0095:Gstm7	UTSW	3	107,837,879 (GRCm39)	splice site	probably benign
R1052:Gstm7	UTSW	3	107,834,266 (GRCm39)	missense	probably benign	0.05
R2121:Gstm7	UTSW	3	107,834,230 (GRCm39)	missense	probably benign	0.00
R4610:Gstm7	UTSW	3	107,834,235 (GRCm39)	missense	possibly damaging	0.65
R5966:Gstm7	UTSW	3	107,838,747 (GRCm39)	intron	probably benign
R6393:Gstm7	UTSW	3	107,838,142 (GRCm39)	critical splice donor site	probably null
R7014:Gstm7	UTSW	3	107,834,278 (GRCm39)	missense	probably benign	0.00
R7052:Gstm7	UTSW	3	107,838,633 (GRCm39)	missense	probably damaging	0.96
R7741:Gstm7	UTSW	3	107,838,963 (GRCm39)	missense	possibly damaging	0.90
R7848:Gstm7	UTSW	3	107,835,902 (GRCm39)	splice site	probably null
R7988:Gstm7	UTSW	3	107,834,271 (GRCm39)	missense	possibly damaging	0.82
R7998:Gstm7	UTSW	3	107,837,657 (GRCm39)	missense	probably damaging	1.00
R8952:Gstm7	UTSW	3	107,838,757 (GRCm39)	intron	probably benign

Predicted Primers

PCR Primer
(F):5'- GGGGACTTTAGCTTTAGAAAGGGCTAC -3'
(R):5'- TATAATGGGCACGATCCCGATGCAC -3'

Sequencing Primer
(F):5'- AGCTTTAGAAAGGGCTACTTTGC -3'
(R):5'- ACAGGCATCTTCGCATAGG -3'

Posted On

2013-11-08