Mutagenetix > Incidental Mutation

Incidental Mutation 'R5786:Or1j4'

447993

Institutional Source

Beutler Lab

Gene Symbol

Or1j4

Ensembl Gene

ENSMUSG00000050015

Gene Name

olfactory receptor family 1 subfamily J member 4

Synonyms

MOR136-13, Olfr350, GA_x6K02T2NLDC-33544602-33545540

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.275) question?

Stock #

R5786 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

36740060-36740998 bp(+) (GRCm39)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

T to C at 36740061 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 1 (M1T)

Ref Sequence

ENSEMBL: ENSMUSP00000149667 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055130] [ENSMUST00000214457] [ENSMUST00000215100]

AlphaFold

Q8VFP8

Predicted Effect

probably null
Transcript: ENSMUST00000055130
AA Change: M1T

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000053105
Gene: ENSMUSG00000050015
AA Change: M1T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	6.3e-61	PFAM
Pfam:7TM_GPCR_Srsx	35	305	1.3e-7	PFAM
Pfam:7tm_1	41	290	3e-24	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000121879

Predicted Effect

probably null
Transcript: ENSMUST00000214457
AA Change: M1T

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

Predicted Effect

probably null
Transcript: ENSMUST00000215100
AA Change: M1T

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.5%
20x: 95.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810009J06Rik	T	A	6: 40,945,122 (GRCm39)	D200E	probably damaging	Het
4930578I06Rik	C	A	14: 64,210,691 (GRCm39)	R179L	probably damaging	Het
Abhd5	A	G	9: 122,192,868 (GRCm39)		probably null	Het
Ankrd60	TGGCCACGCGG	TGG	2: 173,419,882 (GRCm39)		probably null	Het
Ano5	G	A	7: 51,216,066 (GRCm39)	D348N	possibly damaging	Het
Apob	C	T	12: 8,065,304 (GRCm39)	T4091I	possibly damaging	Het
Avil	G	A	10: 126,852,368 (GRCm39)		probably null	Het
Cacna1a	T	C	8: 85,142,350 (GRCm39)		probably benign	Het
Capn7	T	C	14: 31,082,102 (GRCm39)	L436P	probably damaging	Het
Ccdc33	A	G	9: 57,937,235 (GRCm39)	S655P	possibly damaging	Het
Ccr6	T	C	17: 8,475,244 (GRCm39)	S150P	probably damaging	Het
Cd1d1	T	C	3: 86,906,095 (GRCm39)	N60S	probably benign	Het
Ckap5	A	G	2: 91,446,641 (GRCm39)		probably null	Het
Col15a1	A	G	4: 47,280,865 (GRCm39)	E753G	possibly damaging	Het
Col1a2	C	T	6: 4,530,223 (GRCm39)	R699W	unknown	Het
Cracd	T	C	5: 77,014,043 (GRCm39)		probably null	Het
Csf2rb	T	C	15: 78,233,155 (GRCm39)	Y821H	probably damaging	Het
Cyp3a11	A	G	5: 145,799,284 (GRCm39)	I301T	possibly damaging	Het
Dpp3	C	T	19: 4,968,350 (GRCm39)	G241R	possibly damaging	Het
Dpyd	G	T	3: 119,220,886 (GRCm39)	M952I	probably damaging	Het
Dsg3	A	T	18: 20,654,628 (GRCm39)	I111L	possibly damaging	Het
Ect2	A	G	3: 27,201,102 (GRCm39)	F123L	probably damaging	Het
Ehmt2	G	C	17: 35,129,719 (GRCm39)	D961H	probably damaging	Het
Esp1	A	G	17: 41,041,809 (GRCm39)	I34V	probably benign	Het
Fam171b	G	A	2: 83,708,580 (GRCm39)	V361I	probably benign	Het
Flnc	T	A	6: 29,459,536 (GRCm39)	Y2545*	probably null	Het
Fmo4	C	T	1: 162,631,286 (GRCm39)	G227D	probably benign	Het
Grn	C	T	11: 102,324,869 (GRCm39)	Q153*	probably null	Het
H2-DMb1	T	G	17: 34,372,408 (GRCm39)	S12R	possibly damaging	Het
Ica1	G	T	6: 8,672,391 (GRCm39)	N203K	possibly damaging	Het
Kdm4c	C	A	4: 74,277,722 (GRCm39)	T792K	probably damaging	Het
Kif19a	T	A	11: 114,670,049 (GRCm39)	Y81*	probably null	Het
Kifc2	G	T	15: 76,548,578 (GRCm39)	C440F	probably damaging	Het
Lpin2	A	G	17: 71,537,268 (GRCm39)	T234A	probably benign	Het
Lysmd2	C	A	9: 75,542,885 (GRCm39)	P164Q	probably benign	Het
Maea	T	A	5: 33,526,027 (GRCm39)	D234E	probably benign	Het
Map4k1	T	A	7: 28,699,445 (GRCm39)	V572E	probably damaging	Het
Med6	C	T	12: 81,620,733 (GRCm39)	G166R	probably null	Het
Mtmr10	T	C	7: 63,987,458 (GRCm39)	I666T	probably damaging	Het
Myh14	T	A	7: 44,262,887 (GRCm39)	K1777M	probably benign	Het
Naip6	G	T	13: 100,436,724 (GRCm39)	Q600K	probably benign	Het
Obscn	A	G	11: 58,923,517 (GRCm39)	S6461P	probably damaging	Het
Osbpl7	T	A	11: 96,956,658 (GRCm39)	V567E	probably damaging	Het
Rad51ap2	A	T	12: 11,506,921 (GRCm39)	D281V	probably damaging	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Rpl24	C	A	16: 55,787,516 (GRCm39)	H59N	possibly damaging	Het
Rtl1	G	A	12: 109,559,053 (GRCm39)	L929F	possibly damaging	Het
Runx3	C	T	4: 134,890,575 (GRCm39)	T159I	probably damaging	Het
Serpine2	T	C	1: 79,794,637 (GRCm39)	I99V	probably benign	Het
Slc12a6	C	A	2: 112,115,067 (GRCm39)	P12Q	probably benign	Het
Slc25a18	T	C	6: 120,769,035 (GRCm39)	L184P	probably damaging	Het
Smg1	T	C	7: 117,812,120 (GRCm39)	D57G	probably benign	Het
Spdye4c	T	A	2: 128,438,761 (GRCm39)	*340K	probably null	Het
Srsf5	G	A	12: 80,996,311 (GRCm39)	E162K	possibly damaging	Het
Ssc5d	T	C	7: 4,939,817 (GRCm39)	V751A	probably benign	Het
Tcf3	T	C	10: 80,255,333 (GRCm39)	N157S	probably benign	Het
Tdrd7	T	C	4: 45,989,082 (GRCm39)	V71A	probably benign	Het
Tex14	T	C	11: 87,405,121 (GRCm39)	C678R	probably damaging	Het
Tgm3	A	T	2: 129,868,704 (GRCm39)	K214*	probably null	Het
Vps53	A	G	11: 75,953,833 (GRCm39)	I659T	probably benign	Het
Zfp597	A	T	16: 3,684,023 (GRCm39)	C244*	probably null	Het
Zfp933	T	A	4: 147,912,864 (GRCm39)		probably null	Het

Other mutations in Or1j4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01068:Or1j4	APN	2	36,740,282 (GRCm39)	missense	probably damaging	1.00
IGL01113:Or1j4	APN	2	36,740,631 (GRCm39)	missense	probably damaging	1.00
IGL01393:Or1j4	APN	2	36,740,553 (GRCm39)	missense	probably benign	0.01
IGL02302:Or1j4	APN	2	36,740,715 (GRCm39)	missense	probably benign	0.02
IGL02316:Or1j4	APN	2	36,740,294 (GRCm39)	missense	probably damaging	1.00
BB007:Or1j4	UTSW	2	36,740,285 (GRCm39)	missense	probably damaging	1.00
BB017:Or1j4	UTSW	2	36,740,285 (GRCm39)	missense	probably damaging	1.00
F6893:Or1j4	UTSW	2	36,740,819 (GRCm39)	missense	probably benign	0.00
PIT4402001:Or1j4	UTSW	2	36,740,316 (GRCm39)	missense	probably benign
R0312:Or1j4	UTSW	2	36,740,372 (GRCm39)	missense	probably benign	0.01
R0525:Or1j4	UTSW	2	36,740,202 (GRCm39)	missense	probably damaging	1.00
R0557:Or1j4	UTSW	2	36,740,760 (GRCm39)	missense	possibly damaging	0.95
R0665:Or1j4	UTSW	2	36,740,202 (GRCm39)	missense	probably damaging	1.00
R2019:Or1j4	UTSW	2	36,740,418 (GRCm39)	missense	possibly damaging	0.95
R2107:Or1j4	UTSW	2	36,740,355 (GRCm39)	missense	possibly damaging	0.54
R2108:Or1j4	UTSW	2	36,740,355 (GRCm39)	missense	possibly damaging	0.54
R2848:Or1j4	UTSW	2	36,740,811 (GRCm39)	missense	probably damaging	1.00
R3964:Or1j4	UTSW	2	36,740,729 (GRCm39)	missense	probably benign	0.12
R4822:Or1j4	UTSW	2	36,740,888 (GRCm39)	missense	probably benign	0.10
R4907:Or1j4	UTSW	2	36,740,270 (GRCm39)	missense	probably benign	0.28
R5134:Or1j4	UTSW	2	36,740,488 (GRCm39)	missense	probably benign	0.03
R5144:Or1j4	UTSW	2	36,740,156 (GRCm39)	missense	probably benign
R5702:Or1j4	UTSW	2	36,740,946 (GRCm39)	missense	probably damaging	1.00
R5727:Or1j4	UTSW	2	36,740,544 (GRCm39)	missense	possibly damaging	0.80
R6179:Or1j4	UTSW	2	36,740,846 (GRCm39)	missense	possibly damaging	0.59
R6862:Or1j4	UTSW	2	36,740,234 (GRCm39)	missense	possibly damaging	0.95
R7258:Or1j4	UTSW	2	36,740,352 (GRCm39)	missense	probably damaging	0.99
R7307:Or1j4	UTSW	2	36,740,137 (GRCm39)	missense	probably benign	0.00
R7353:Or1j4	UTSW	2	36,740,081 (GRCm39)	missense	probably benign
R7412:Or1j4	UTSW	2	36,740,478 (GRCm39)	missense	probably benign	0.28
R7851:Or1j4	UTSW	2	36,740,428 (GRCm39)	nonsense	probably null
R7930:Or1j4	UTSW	2	36,740,285 (GRCm39)	missense	probably damaging	1.00
R8005:Or1j4	UTSW	2	36,740,156 (GRCm39)	missense	probably benign
R8346:Or1j4	UTSW	2	36,740,351 (GRCm39)	missense	probably benign	0.02
R8692:Or1j4	UTSW	2	36,740,096 (GRCm39)	missense	probably benign	0.00
R9120:Or1j4	UTSW	2	36,740,143 (GRCm39)	nonsense	probably null
R9318:Or1j4	UTSW	2	36,740,565 (GRCm39)	missense	probably benign	0.12
Z1177:Or1j4	UTSW	2	36,740,937 (GRCm39)	missense	probably damaging	1.00
Z1177:Or1j4	UTSW	2	36,740,251 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TCCCCAGATGCTGTTGAATG -3'
(R):5'- ATGTCTGTGAAGGCCAAGTG -3'

Sequencing Primer
(F):5'- CCCCAGATGCTGTTGAATGTTAATG -3'
(R):5'- CCAAGTGGCTGAGGAAGAAGTAC -3'

Posted On

2016-12-15