Mutagenetix > Incidental Mutation

Incidental Mutation 'R5731:Vmn2r28'

452757

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r28

Ensembl Gene

ENSMUSG00000066820

Gene Name

vomeronasal 2, receptor 28

Synonyms

EG665255

MMRRC Submission

043192-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.124) question?

Stock #

R5731 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

5483455-5496850 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 5491668 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Lysine at position 193 (T193K)

Ref Sequence

ENSEMBL: ENSMUSP00000083477 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086297]

AlphaFold

L7N203

Predicted Effect

probably benign
Transcript: ENSMUST00000086297
AA Change: T193K

PolyPhen 2 Score 0.409 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000083477
Gene: ENSMUSG00000066820
AA Change: T193K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	470	1.7e-26	PFAM
Pfam:NCD3G	512	565	9.8e-21	PFAM
Pfam:7tm_3	598	833	2.8e-56	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5930422O12Rik	A	G	8: 33,919,353 (GRCm39)	I58V	unknown	Het
Abca4	G	T	3: 121,926,242 (GRCm39)	G18V	probably damaging	Het
Arl9	T	C	5: 77,154,374 (GRCm39)	V34A	possibly damaging	Het
Bpifb9a	A	T	2: 154,104,163 (GRCm39)	N202I	possibly damaging	Het
C1qtnf6	A	T	15: 78,411,514 (GRCm39)	M54K	probably benign	Het
Ccdc62	T	C	5: 124,089,352 (GRCm39)		probably null	Het
Cd177	A	G	7: 24,443,846 (GRCm39)	C751R	probably damaging	Het
Clcn3	T	C	8: 61,375,923 (GRCm39)	I657V	possibly damaging	Het
Cmtm1	CGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGT	CGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGT	8: 105,036,102 (GRCm39)		probably benign	Het
Dag1	T	C	9: 108,095,310 (GRCm39)	T61A	probably benign	Het
Fam184b	C	T	5: 45,710,471 (GRCm39)	G553E	probably benign	Het
Fkbp15	G	C	4: 62,225,166 (GRCm39)	A831G	probably benign	Het
Flt1	T	C	5: 147,614,962 (GRCm39)	H328R	probably benign	Het
Fmnl2	T	A	2: 53,008,149 (GRCm39)		probably null	Het
Gm5431	A	G	11: 48,785,275 (GRCm39)	Y89H	probably damaging	Het
Gprin2	C	A	14: 33,917,397 (GRCm39)	L124F	probably damaging	Het
Hacd2	T	A	16: 34,922,374 (GRCm39)	Y188N	probably damaging	Het
Itgad	A	G	7: 127,797,726 (GRCm39)	T950A	probably benign	Het
Kit	T	A	5: 75,815,075 (GRCm39)	I933N	possibly damaging	Het
Klhl11	A	T	11: 100,354,589 (GRCm39)	Y411N	probably damaging	Het
N4bp2	T	C	5: 65,966,500 (GRCm39)	S1313P	probably damaging	Het
Neurog1	T	C	13: 56,399,354 (GRCm39)	K131R	probably damaging	Het
Or11h6	T	C	14: 50,880,248 (GRCm39)	L170P	probably damaging	Het
Or5d16	T	A	2: 87,773,771 (GRCm39)	H67L	possibly damaging	Het
Otogl	T	C	10: 107,717,325 (GRCm39)	D382G	probably damaging	Het
Pcdh1	A	C	18: 38,331,651 (GRCm39)	F590V	probably damaging	Het
Pcdha5	G	A	18: 37,093,820 (GRCm39)	V110M	probably damaging	Het
Pdlim3	A	G	8: 46,368,284 (GRCm39)	N261D	probably benign	Het
Pou4f1	T	C	14: 104,703,347 (GRCm39)	T362A	unknown	Het
Prlr	A	G	15: 10,314,221 (GRCm39)	T9A	probably benign	Het
Psmb7	T	C	2: 38,478,289 (GRCm39)	Y245C	probably damaging	Het
Ryr3	T	A	2: 112,471,917 (GRCm39)	D4515V	probably damaging	Het
Svop	T	C	5: 114,198,124 (GRCm39)	K149E	probably damaging	Het
Tlr3	A	G	8: 45,851,157 (GRCm39)	V56A	probably benign	Het
Tm4sf20	T	G	1: 82,738,013 (GRCm39)	I93L	probably benign	Het
Ugt2b5	T	A	5: 87,288,111 (GRCm39)	R19*	probably null	Het
Vps13c	A	G	9: 67,802,661 (GRCm39)	D654G	probably damaging	Het
Zfyve16	T	A	13: 92,644,701 (GRCm39)	Q1167L	probably benign	Het

Other mutations in Vmn2r28

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Vmn2r28	APN	7	5,491,068 (GRCm39)	missense	probably benign	0.12
IGL01061:Vmn2r28	APN	7	5,491,183 (GRCm39)	missense	probably damaging	1.00
IGL01160:Vmn2r28	APN	7	5,489,477 (GRCm39)	missense	probably damaging	0.99
IGL01335:Vmn2r28	APN	7	5,484,087 (GRCm39)	missense	possibly damaging	0.67
IGL01532:Vmn2r28	APN	7	5,489,463 (GRCm39)	missense	probably benign	0.16
IGL01791:Vmn2r28	APN	7	5,491,161 (GRCm39)	missense	probably benign	0.00
IGL01875:Vmn2r28	APN	7	5,484,302 (GRCm39)	missense	probably benign	0.30
IGL02161:Vmn2r28	APN	7	5,491,123 (GRCm39)	missense	possibly damaging	0.88
IGL02499:Vmn2r28	APN	7	5,493,568 (GRCm39)	missense	probably damaging	0.98
IGL02858:Vmn2r28	APN	7	5,484,003 (GRCm39)	missense	probably damaging	0.99
IGL03061:Vmn2r28	APN	7	5,487,015 (GRCm39)	missense	probably damaging	0.98
G1patch:Vmn2r28	UTSW	7	5,491,408 (GRCm39)	missense	probably benign	0.13
R0288:Vmn2r28	UTSW	7	5,491,020 (GRCm39)	missense	probably damaging	1.00
R0361:Vmn2r28	UTSW	7	5,496,715 (GRCm39)	missense	probably benign	0.00
R0396:Vmn2r28	UTSW	7	5,491,513 (GRCm39)	missense	probably benign	0.05
R0480:Vmn2r28	UTSW	7	5,493,456 (GRCm39)	missense	probably benign	0.00
R0485:Vmn2r28	UTSW	7	5,491,689 (GRCm39)	missense	probably damaging	1.00
R0837:Vmn2r28	UTSW	7	5,491,026 (GRCm39)	missense	probably damaging	0.99
R1282:Vmn2r28	UTSW	7	5,484,301 (GRCm39)	missense	probably damaging	0.99
R1296:Vmn2r28	UTSW	7	5,484,544 (GRCm39)	missense	possibly damaging	0.81
R1829:Vmn2r28	UTSW	7	5,496,810 (GRCm39)	missense	probably benign
R1853:Vmn2r28	UTSW	7	5,484,246 (GRCm39)	nonsense	probably null
R1869:Vmn2r28	UTSW	7	5,489,345 (GRCm39)	missense	probably benign	0.00
R1887:Vmn2r28	UTSW	7	5,491,288 (GRCm39)	missense	possibly damaging	0.90
R1961:Vmn2r28	UTSW	7	5,484,070 (GRCm39)	missense	possibly damaging	0.85
R1998:Vmn2r28	UTSW	7	5,491,313 (GRCm39)	missense	possibly damaging	0.87
R2392:Vmn2r28	UTSW	7	5,487,130 (GRCm39)	missense	probably damaging	0.98
R2432:Vmn2r28	UTSW	7	5,491,701 (GRCm39)	missense	probably damaging	0.99
R3055:Vmn2r28	UTSW	7	5,484,391 (GRCm39)	missense	probably damaging	0.98
R3753:Vmn2r28	UTSW	7	5,491,026 (GRCm39)	missense	probably damaging	0.99
R3877:Vmn2r28	UTSW	7	5,491,357 (GRCm39)	missense	probably damaging	1.00
R4307:Vmn2r28	UTSW	7	5,493,707 (GRCm39)	missense	probably damaging	0.99
R5023:Vmn2r28	UTSW	7	5,489,463 (GRCm39)	missense	probably benign	0.16
R5057:Vmn2r28	UTSW	7	5,489,463 (GRCm39)	missense	probably benign	0.16
R5083:Vmn2r28	UTSW	7	5,483,671 (GRCm39)	missense	possibly damaging	0.77
R5427:Vmn2r28	UTSW	7	5,489,376 (GRCm39)	missense	probably damaging	0.99
R5472:Vmn2r28	UTSW	7	5,490,943 (GRCm39)	critical splice donor site	probably null
R5511:Vmn2r28	UTSW	7	5,487,011 (GRCm39)	missense	possibly damaging	0.61
R6091:Vmn2r28	UTSW	7	5,496,790 (GRCm39)	missense	possibly damaging	0.88
R6179:Vmn2r28	UTSW	7	5,491,003 (GRCm39)	nonsense	probably null
R6276:Vmn2r28	UTSW	7	5,493,730 (GRCm39)	missense	probably benign	0.01
R6441:Vmn2r28	UTSW	7	5,491,474 (GRCm39)	missense	probably benign	0.00
R6463:Vmn2r28	UTSW	7	5,489,435 (GRCm39)	missense	probably benign	0.07
R6528:Vmn2r28	UTSW	7	5,493,684 (GRCm39)	missense	probably benign	0.12
R6725:Vmn2r28	UTSW	7	5,491,408 (GRCm39)	missense	probably benign	0.13
R6760:Vmn2r28	UTSW	7	5,484,229 (GRCm39)	missense	probably damaging	0.97
R6849:Vmn2r28	UTSW	7	5,483,806 (GRCm39)	missense	probably damaging	1.00
R7110:Vmn2r28	UTSW	7	5,493,733 (GRCm39)	missense	probably benign	0.06
R7146:Vmn2r28	UTSW	7	5,484,495 (GRCm39)	missense	probably benign	0.05
R7407:Vmn2r28	UTSW	7	5,484,308 (GRCm39)	missense	probably damaging	1.00
R7563:Vmn2r28	UTSW	7	5,491,200 (GRCm39)	missense	probably benign	0.00
R7611:Vmn2r28	UTSW	7	5,484,255 (GRCm39)	missense	probably benign	0.02
R7808:Vmn2r28	UTSW	7	5,496,678 (GRCm39)	missense	probably damaging	0.98
R7862:Vmn2r28	UTSW	7	5,493,613 (GRCm39)	missense	probably benign	0.00
R7916:Vmn2r28	UTSW	7	5,483,818 (GRCm39)	missense	probably damaging	1.00
R8183:Vmn2r28	UTSW	7	5,491,147 (GRCm39)	missense	probably damaging	1.00
R8334:Vmn2r28	UTSW	7	5,487,059 (GRCm39)	missense	probably damaging	1.00
R8519:Vmn2r28	UTSW	7	5,489,347 (GRCm39)	missense	probably benign	0.31
R8989:Vmn2r28	UTSW	7	5,493,473 (GRCm39)	missense	probably benign	0.00
R9066:Vmn2r28	UTSW	7	5,491,596 (GRCm39)	missense	probably damaging	1.00
R9422:Vmn2r28	UTSW	7	5,483,747 (GRCm39)	missense	probably damaging	1.00
R9469:Vmn2r28	UTSW	7	5,487,118 (GRCm39)	missense	probably damaging	0.99
R9608:Vmn2r28	UTSW	7	5,491,220 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- TGTGGCATAGGTCAAAATATCAGAG -3'
(R):5'- TGCCAGGATTGAACATTTGCTC -3'

Sequencing Primer
(F):5'- GAGATTCCTATCATATTCACAAAGGC -3'
(R):5'- GCCAGGATTGAACATTTGCTCATTTG -3'

Posted On

2017-01-03