Mutagenetix > Incidental Mutation

Incidental Mutation 'R5855:Lrrc27'

454870

Institutional Source

Beutler Lab

Gene Symbol

Lrrc27

Ensembl Gene

ENSMUSG00000015980

Gene Name

leucine rich repeat containing 27

Synonyms

1700071K18Rik, 2310044E02Rik

MMRRC Submission

043229-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R5855 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

139212988-139242979 bp(+) (GRCm38)

Type of Mutation

unclassified

DNA Base Change (assembly)

T to C at 139218335 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000116624 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000016124] [ENSMUST00000106104] [ENSMUST00000135509] [ENSMUST00000156768]

AlphaFold

Q80YS5

Predicted Effect

silent
Transcript: ENSMUST00000016124

SMART Domains

Protein: ENSMUSP00000016124
Gene: ENSMUSG00000015980

Domain	Start	End	E-Value	Type
low complexity region	8	32	N/A	INTRINSIC
LRR_TYP	75	98	1.03e-2	SMART
LRR_TYP	99	122	3.69e-4	SMART
LRR	123	145	7.38e1	SMART
low complexity region	271	283	N/A	INTRINSIC
coiled coil region	336	370	N/A	INTRINSIC
coiled coil region	463	491	N/A	INTRINSIC

Predicted Effect

silent
Transcript: ENSMUST00000106104

SMART Domains

Protein: ENSMUSP00000101710
Gene: ENSMUSG00000015980

Domain	Start	End	E-Value	Type
low complexity region	8	32	N/A	INTRINSIC
LRR_TYP	75	98	1.03e-2	SMART
LRR_TYP	99	122	3.69e-4	SMART
LRR	123	145	7.38e1	SMART
low complexity region	271	283	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126199

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130248

Predicted Effect

unknown
Transcript: ENSMUST00000135509
AA Change: S163P

SMART Domains

Protein: ENSMUSP00000116827
Gene: ENSMUSG00000015980
AA Change: S163P

Domain	Start	End	E-Value	Type
low complexity region	8	32	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000156768

SMART Domains

Protein: ENSMUSP00000116624
Gene: ENSMUSG00000015980

Domain	Start	End	E-Value	Type
low complexity region	8	32	N/A	INTRINSIC
LRR_TYP	75	98	1.03e-2	SMART

Coding Region Coverage

1x: 99.8%
3x: 99.4%
10x: 96.8%
20x: 89.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acadsb	T	G	7: 131,424,599	L57R	probably damaging	Het
Bmpr1b	G	A	3: 141,871,385	T55M	possibly damaging	Het
Cep350	G	A	1: 155,953,762	T132I	probably benign	Het
Cops4	A	T	5: 100,547,414	M400L	probably benign	Het
Cul1	G	A	6: 47,523,213	D653N	probably benign	Het
Cyp3a13	C	G	5: 137,919,056	L36F	probably damaging	Het
Dcaf10	T	C	4: 45,342,558	F131L	probably benign	Het
Dgkh	T	C	14: 78,624,504		probably null	Het
Igll1	C	T	16: 16,861,057	V130M	probably damaging	Het
Kif2c	C	T	4: 117,182,542		probably benign	Het
Klra7	T	C	6: 130,218,958	D262G	possibly damaging	Het
Maf	A	G	8: 115,705,792	S358P	probably benign	Het
Map1a	A	G	2: 121,303,674	D1419G	possibly damaging	Het
Map3k1	G	A	13: 111,755,979	A914V	probably benign	Het
Naa25	T	G	5: 121,423,692	L436R	possibly damaging	Het
Ndc1	T	C	4: 107,383,707	I294T	probably damaging	Het
Nek1	A	C	8: 61,016,272	D121A	probably damaging	Het
Nfil3	C	T	13: 52,968,710	G53R	probably benign	Het
Olfr63	T	C	17: 33,269,336	V204A	possibly damaging	Het
Parp14	A	T	16: 35,840,927	Y1550*	probably null	Het
Patl1	A	G	19: 11,921,516	I192V	probably damaging	Het
Pax3	G	A	1: 78,121,651	T367I	probably damaging	Het
Pla2g4a	C	T	1: 149,880,063	V208M	probably damaging	Het
Prdm10	A	T	9: 31,337,323	K347M	probably damaging	Het
Prkd1	A	T	12: 50,392,916	M376K	probably benign	Het
Prkg1	C	T	19: 30,894,694	V219I	possibly damaging	Het
Rictor	G	A	15: 6,794,006	E1555K	probably benign	Het
Scn3a	T	C	2: 65,464,730	I1550V	possibly damaging	Het
Skint8	C	A	4: 111,950,193	L359M	probably damaging	Het
Sox4	T	C	13: 28,952,996	E9G	probably damaging	Het
Spon1	T	A	7: 114,029,072	D354E	probably damaging	Het
Stat2	T	A	10: 128,283,494	L450H	probably damaging	Het
Tek	T	A	4: 94,853,553	M849K	probably damaging	Het
Tmem63c	G	A	12: 87,075,726	D433N	probably damaging	Het
Tnpo3	G	A	6: 29,589,033	T106I	probably damaging	Het
Tns1	G	T	1: 73,918,033	A1674D	possibly damaging	Het
Trim8	T	C	19: 46,515,410	V467A	possibly damaging	Het
Trmo	T	A	4: 46,382,568	H183L	probably benign	Het
Trpm1	C	A	7: 64,268,962	C683*	probably null	Het
Vsig10	T	G	5: 117,338,270	L263R	probably damaging	Het
Zfp874a	A	T	13: 67,442,693	Y291N	probably benign	Het

Other mutations in Lrrc27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01668:Lrrc27	APN	7	139227911	intron	probably benign
IGL02095:Lrrc27	APN	7	139230253	missense	probably benign	0.04
IGL02489:Lrrc27	APN	7	139226061	missense	probably benign	0.01
IGL03080:Lrrc27	APN	7	139230237	missense	probably benign	0.03
R0372:Lrrc27	UTSW	7	139226187	missense	probably benign	0.17
R1466:Lrrc27	UTSW	7	139230308	unclassified	probably benign
R2401:Lrrc27	UTSW	7	139223613	missense	probably damaging	1.00
R2876:Lrrc27	UTSW	7	139228684	intron	probably benign
R3113:Lrrc27	UTSW	7	139218307	missense	probably damaging	1.00
R4214:Lrrc27	UTSW	7	139223693	missense	probably damaging	1.00
R4707:Lrrc27	UTSW	7	139242698	missense	probably benign	0.02
R4784:Lrrc27	UTSW	7	139242698	missense	probably benign	0.02
R5070:Lrrc27	UTSW	7	139214799	missense	probably damaging	0.99
R6408:Lrrc27	UTSW	7	139218268	missense	probably benign	0.14
R6993:Lrrc27	UTSW	7	139242624	missense	probably damaging	0.99
R7332:Lrrc27	UTSW	7	139242745	missense	probably damaging	1.00
R7350:Lrrc27	UTSW	7	139226106	missense	probably benign	0.01
R7460:Lrrc27	UTSW	7	139223658	missense	probably damaging	1.00
R7502:Lrrc27	UTSW	7	139214832	missense	probably benign
R8020:Lrrc27	UTSW	7	139236877	missense	probably damaging	1.00
R8071:Lrrc27	UTSW	7	139236986	missense	probably benign	0.01
R8518:Lrrc27	UTSW	7	139228774	missense	probably benign	0.01
R8728:Lrrc27	UTSW	7	139242639	missense	probably damaging	1.00
R8734:Lrrc27	UTSW	7	139216599	unclassified	probably benign
R9141:Lrrc27	UTSW	7	139227945	missense	probably benign	0.03
R9355:Lrrc27	UTSW	7	139242732	missense	probably damaging	0.98
R9387:Lrrc27	UTSW	7	139227921	nonsense	probably null
RF018:Lrrc27	UTSW	7	139226100	missense	probably benign	0.03
X0065:Lrrc27	UTSW	7	139230245	missense	probably benign	0.00
X0065:Lrrc27	UTSW	7	139230246	missense	probably benign	0.00
Z1176:Lrrc27	UTSW	7	139242720	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGCTGAGTGTAGCCAGAGAG -3'
(R):5'- CTGCACTTTGAACCATCTGGAC -3'

Sequencing Primer
(F):5'- AATCTCTCTGGTTCTTGAGTCCG -3'
(R):5'- GGACTTTTTAATTCTGAGACGGCAAC -3'

Posted On

2017-02-10